TY - JOUR A1 - Giménez, C. A1 - Climent Terol, Estela A1 - Aznar, E. A1 - Martínez-Mánez, Ramon A1 - Sancenón, F. A1 - Marcos, D. A1 - Amorós, P. A1 - Rurack, Knut T1 - Towards chemical communication between gated nanoparticles N2 - The design of comparatively simple and modularly configurable artificial systems able to communicate through the exchange of chemical messengers is, to the best of our knowledge, an unexplored field. As a proof-of-concept, we present here a family of nanoparticles that have been designed to communicate with one another in a hierarchical manner. The concept involves the use of capped mesoporous silica supports in which the messenger delivered by a first type of gated nanoparticle is used to open a second type of nanoparticle, which delivers another messenger that opens a third group of gated nanoobjects. We believe that the conceptual idea that nanodevices can be designed to communicate with one another may result in novel applications and will boost further advances towards cooperative systems with complex behavior as a result of the communication between simple abiotic individual components. KW - Chemical communication KW - Gated nanoparticles KW - Mesoporous materials KW - Nanoparticles community KW - Nanoparticles PY - 2014 DO - https://doi.org/10.1002/anie.201405580 SN - 1433-7851 SN - 1521-3773 SN - 0570-0833 VL - 53 IS - 46 SP - 12629 EP - 12633 PB - Wiley-VCH CY - Weinheim AN - OPUS4-32136 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Tobias, Charlie A1 - Climent Terol, Estela A1 - Gawlitza, Kornelia A1 - Rurack, Knut T1 - Polystyrene Microparticles with Convergently Grown Mesoporous Silica Shells as a Promising Tool for Multiplexed Bioanalytical Assays N2 - Functional core/shell particles are highly sought after in analytical chemistry, especially in methods suitable for single-particle analysis such as flow cytometry because they allow for facile multiplexed detection of several analytes in a single run. Aiming to develop a powerful bead platform of which the core particle can be doped in a straightforward manner while the shell offers the highest possible sensitivity when functionalized with (bio)chemical binders, polystyrene particles were coated with different kinds of mesoporous silica shells in a convergent growth approach. Mesoporous shells allow us to obtain distinctly higher surface areas in comparison with conventional nonporous shells. While assessing the potential of narrow- as well as wide-pore silicas such as Mobil composition of matter no. 41 (MCM-41) and Santa Barbara amorphous material no. 15 (SBA-15), especially the synthesis of the latter shells that are much more suitable for biomolecule anchoring was optimized by altering the pH and both, the amount and type of the mediator salt. Our studies showed that the best performing material resulted from a synthesis using neutral conditions and MgSO4 as an ionic mediator. The analytical potential of the particles was investigated in flow cytometric DNA assays after their respective functionalization for individual and multiplexed detection of short oligonucleotide strands. These experiments revealed that a two-step modification of the silica surface with amino silane and succinic anhydride prior to coupling of an amino-terminated capture DNA (c-DNA) strand is superior to coupling carboxylic acid-terminated c-DNA to aminated core/shell particles, yielding limits of detection (LOD) down to 5 pM for a hybridization assay, using labeled complementary single-stranded target DNA (t-DNA) 15mers. The potential of the use of the particles in multiplexed analysis was shown with the aid of dye-doped core particles carrying a respective SBA-15 shell. Characteristic genomic sequences of human papillomaviruses (HPV) were chosen as the t-DNA analytes here, since their high relevance as carcinogens and the high number of different pathogens is a relevant model case. The title particles showed a promising performance and allowed us to unequivocally detect the different high- and low-risk HPV types in a single experimental run. KW - Bead-based assay KW - Core-shell particles KW - Human papillomavirus KW - Mesoporous silica KW - Multiplexing PY - 2020 DO - https://doi.org/10.1021/acsami.0c17940 SN - 1944-8244 VL - 13 IS - 1 SP - 207 EP - 218 PB - American Chemical Society CY - Washington, DC AN - OPUS4-51955 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Climent Terol, Estela A1 - Hecht, Mandy A1 - Witthuhn, Heike A1 - Gawlitza, Kornelia A1 - Rurack, Knut T1 - Mix‐&‐read determination of Mercury(II) at trace levels with hybrid mesoporous silica materials incorporating fluorescent probes by a simple mix‐&‐load technique N2 - The synthesis, characterization, and application of mesoporous materials containing boron–dipyrromethene (BODIPY) moieties that allow the sensitive and selective detection of HgII in aqueous environments by fluorescence enhancement is reported. For this purpose, BODIPY dye I containing a thia‐aza crown ether receptor as the fluorescent probe for the detection of HgII in aqueous environments is encapsulated into mesoporous materials to avoid self‐quenching or aggregation in water. Determination of HgII is accomplished within a few seconds with high selectivity and sensitivity, reaching a limit of detection of 12 ppt. The determination of trace amounts of HgII in natural waters and in fish extracts is demonstrated by using our sensing material. The incorporation of the material into several μ‐PAD strips yields a portable, cheap, quick, and easy‐to‐handle tool for trace HgII analysis in water. KW - Dyes/pigments KW - Test strips KW - Mesoporous materials KW - Mercury KW - Fluorescence PY - 2018 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-460138 DO - https://doi.org/10.1002/open.201800277 SN - 2191-1363 VL - 7 IS - 12 SP - 957 EP - 968 PB - Wiley-VCH CY - Weinheim AN - OPUS4-46013 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Climent Terol, Estela A1 - Hecht, Mandy A1 - Witthuhn, Heike A1 - Gawlitza, Kornelia A1 - Rurack, Knut T1 - Cover profile for the article "Mix‐&‐read determination of Mercury(II) at trace levels with hybrid mesoporous silica materials incorporating fluorescent probes by a simple mix‐&‐load technique" N2 - Invited for this month’s cover picture is the group of Dr. Knut Rurack at the Department of Analytical Chemistry; Reference Materials at the Bundesanstalt fuer Materialforschung und -pruefung (BAM) in Berlin (Germany). The cover picture shows how differences in color and fluorescence on a test strip can be easily read out with a mobile device. Two reference spots Frame the sensitive spot that indicates the presence of trace amounts of HgII below the threshold in a natural water sample. This dipstick contains a hybrid material that combines boron-dipyrromethene (BODIPY) probes sterically loaded into specifically tailored mesoporous silica particles, allowing for ultrasensitive HgII detection through enhanced fluorescence in a few seconds. The applicability in real water samples and fish extracts are also studied. KW - Mercury KW - Fluorescence KW - Dip-stick assay KW - Group profile PY - 2018 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-471267 DO - https://doi.org/10.1002/open.201800231 SN - 2191-1363 VL - 7 IS - 12 SP - 932 EP - 933 PB - Wiley-VCH CY - Weinheim AN - OPUS4-47126 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Climent Terol, Estela A1 - Rurack, Knut T1 - Streifenschnelltest mit ppt-Empfindlichkeit durch Kombination von Elektrochemilumineszenz-Detektion mit Aptamer-gesteuerter Indikatorfreisetzung aus mesoporösen Nanopartikeln N2 - Die Kombination aus elektrogenerierter Chemilumineszenz (ECL) und Aptamer-gesteuerter Indikatorfreisetzung (gAID) aus magnetischen mesoporösen Silica-Nanopartikeln, die in mit Poly(ethylenglycol) und N-(3-Triethoxysilylpropyl) diethanolamin funktionalisiertes Glasfaserpapier eingebettet sind, ermöglichte die Entwicklung eines Schnelltests, mit dem sich Penicillin direkt in verdünnter Milch bis auf 50 +/- 9 ppt in <5 min nachweisen lässt. Die kovalente Bindung der Aptamer-Kappe an das Siliciumdioxid-Gerüst ermöglichte den Verschluss der Poren durch nicht-kovalente elektrostatische Wechselwirkungen mit Oberflächen-Aminogruppen. Die Bindung von Penicillin führte zu einer Faltung des Aptamers, wodurch der zuvor in das Material geladene ECL-Reporter Ru(bpy)32+ freigesetzt wurde und nach lateralem Fluss von einer Smartphone-Kamera nach elektrochemischer Anregung mit einer in einen 3D-gedruckten Halter eingesetzten Siebdruckelektrode detektiert wird. Der Ansatz ist einfach, generisch und bietet Vorteile hinsichtlich Empfindlichkeit, Messunsicherheit und Robustheit gegenüber konventioneller Fluoreszenz- oder elektrochemischer Detektion, insbesondere für Point-of-Need-Analysen anspruchsvoller Matrices und Analyten im Ultraspurenbereich. KW - Schnelltests KW - Elektrochemilumineszenz KW - Aptamere KW - Teststreifen KW - Gesteuerte Freisetzung PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-540205 DO - https://doi.org/10.1002/ange.202110744 VL - 133 IS - 50 SP - 26491 EP - 26501 PB - Wiley-VCH CY - Weinheim AN - OPUS4-54020 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Garrido, E. A1 - Climent Terol, Estela A1 - Marcos, M. D. A1 - Sancenón, F. A1 - Rurack, Knut A1 - Martínez-Máñez, R. T1 - Dualplex lateral flow assay for simultaneous scopolamine and "cannibal drug" detection based on receptor-gated mesoporous nanoparticles N2 - We report herein the design of a strip-based rapid test utilizing bioinspired hybrid nanomaterials for the in situ and at site detection of the drug scopolamine (SCP) using a smartphone for readout, allowing SCP identification in diluted saliva down to 40 nM in less than 15 min. For this purpose, we prepared a nanosensor based on mesoporous silica nanoparticles loaded with a fluorescent reporter (rhodamine B) and functionalized with bethanechol, a potent agonist of recombinant human muscarinic acetylcholine receptor M2 (M2-AChR). M2-AChR interaction with the anchored bethanechol derivative leads to capping of the pores. The sensing mechanism relies on binding of SCP to M2-AChR resulting in pore opening and delivery of the entrapped rhodamine B reporter. Moreover, the material was incorporated into strips for lateral-flow assays coupled to smartphone readout, giving fast response time, good selectivity, and exceptional sensitivity. In an attempt to a mobile analytical test system for law enforcement services, we have also developed a dualplex lateral flow assay for SCP and 3,4-methylenedioxypyrovalerone (MDPV) also known as the so-called “cannibal drug”. KW - Rapid tests KW - Vor-Ort-Analytik KW - Lateral Flow Assays KW - Scopolamine KW - Gesteuerte Freisetzung KW - Cannibal Drug PY - 2022 DO - https://doi.org/10.1039/d2nr03325a SP - 1 EP - 9 PB - Royal Society of Chemistry CY - Cambridge AN - OPUS4-55744 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Climent Terol, Estela A1 - Biyikal, Mustafa A1 - Gawlitza, Kornelia A1 - Dropa, T. A1 - Urban, M. A1 - Costero, A. M. A1 - Martínez-Mánez, Ramon A1 - Rurack, Knut T1 - A rapid and sensitive strip-based quick test for nerve agents Tabun, Sarin, and Soman using BODIPY-modified silica materials N2 - Test strips that in combination with a portable fluorescence reader or digital camera can rapidly and selectively detect chemical warfare agents (CWAs) such as Tabun (GA), Sarin (GB), and Soman (GD) and their simulants in the gas phase have been developed. The strips contain spots of a hybrid indicator material consisting of a fluorescent BODIPY indicator covalently anchored into the channels of mesoporous SBA silica microparticles. The fluorescence quenching response allows the sensitive detection of CWAs in the μg m−3 range in a few seconds. KW - Chemical warfare agents KW - Fluorescence KW - Hybrid sensor materials KW - Nerve gases KW - Test strip analysis PY - 2016 DO - https://doi.org/10.1002/chem.201601269 SN - 0947-6539 SN - 1521-3765 VL - 22 IS - 32 SP - 11138 EP - 11142 PB - Wiley CY - Online Library AN - OPUS4-37063 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Climent Terol, Estela T1 - Rapid, sensitive and selective fluorescence sensing device for the determination of the nerve agents Sarin, Soman and Tabun in the gas phase N2 - The organophosphate nerve agents Tabun (GA), Sarin (GB) and Soman (GD) are among the most toxic chemical warfare agents (CWA) known, and exert their biological effects by irreversibly inhibiting acetylcholinesterase enzymes of the human nerve system. The use of these agents in the past century has killed millions of civilians around the world during World Wars I and II, and after that, approximately 40.000 tons of chemical ammunition were dumped into the Baltic Sea, containing ca. 13.000 tons of chemical warfare agents. Since their production, almost all the nations of the world have been strictly avoiding the development and use of CWA, participating in active destruction of CWA stockpiles, especially since the Chemical Weapon Convention (CWC) of 1993. However, CWA have been used in offensive ways against civilian population by terrorists, as for instance in the fatal Tokyo subway terror incident of 1995 or, most recently, against antigovernment demonstrators in Syria in 2013. Due to their higher toxicity and continuous use, it is therefore very important to develop simple and fast detection methods relying on new nerve agent sensing modalities for use in control and inspection. A practically useful fluorescent probe must possess a rapid response and high sensitivity, and shall be implementable into easy-to-use devices for real time detection by untrained personnel. Taking into account this fact, in this work we have synthesized several mesoporous silica materials containing boron–dipyrromethene (BODIPY) moieties for the detection of nerve agents GA, GB and GD in the gas phase. Development of our system indicated that the most potent materials are able to respond to the presence of nerve agent simulants diethyl cyanophosphonate, diethyl chlorophosphate and diisopropyl fluorophosphate, which have to be used in a laboratory setting, yet also for the real nerve agents Sarin, Soman and Tabun, producing a strong quenching of the fluorescence. Furthermore, a portable device for the detection of GA, GB and GD in the gas phase has been prepared for in situ sensing and rapid screening applications, consisting of strips that are able to indicate the targets down to below 1 mgm-3 which is below the LD50 values. T2 - XIII Europtrode Conference CY - Graz, Austria DA - 20.03.2016 KW - nerve agent KW - mesoporous material KW - sensor PY - 2016 AN - OPUS4-35921 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Climent Terol, Estela T1 - Rapid, sensitive and selective determination of nerve agent simulants in the gas phase employing BODIPY-modified silica materials T2 - MAF 14, 14th Conference on methods and applications of fluorescence CY - Würzburg, Germany DA - 2015-09-13 PY - 2015 AN - OPUS4-34659 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Climent Terol, Estela T1 - Integration of novel specific gated hybrid materials into versatile platforms for advanced sensing applications T2 - Network Meeting of the Alexander von Humboldt Fundation CY - Bremen DA - 2015-04-15 PY - 2015 AN - OPUS4-33541 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -