TY - JOUR A1 - Knabe, Ch. A1 - Berger, Georg A1 - Gildenhaar, Renate A1 - Howlett, C.R. A1 - Markovic, B. A1 - Zreiqat, H. T1 - The functional expression of human bone-derived cells grown on rapidly resorbable calcium phosphate ceramics JF - Biomaterials N2 - The use of biodegradable bone substitutes is advantageous for alveolar ridge augmentation, since it avoids second-site surgery for autograft harvesting. This study examines the effect of novel, rapidly resorbable calcium phosphates on the expression of bone-related genes and proteins by human bone-derived cells (HBDC) and compares this behavior to that of tricalciumphosphate (TCP). Test materials were a-TCP, and four materials which were created from ß-Rhenanite and its derivatives: R1-ß-Rhenanite (CaNaPO4); R1/M2 composed of CaNaPO4 and MgNaPO4; R1+SiO2 composed of CaNaPO4 and 9% SiO2 (wt%); and R17-Ca2KNa(PO4)2. HBDC were grown on the substrata for 3, 5, 7, 14 and 21 days, counted and probed for various mRNAs and proteins (Type I collagen, osteocalcin, osteopontin, osteonectin, alkaline phosphatase and bone sialoprotein). All substrata supported continuous cellular growth for 21 days. At day 21, surfaces of R1+SiO2 and R17 had the highest number of HBDC. At 14 and 21 days, cells on R1 and on R1+SiO2 displayed significantly enhanced expression of all osteogenic proteins. Since all novel calcium phosphates supported cellular proliferation together with expression of bone-related proteins at least as much as TCP, these ceramics can be regarded as potential bone substitutes. R1 and R1+SiO2 had the most effect on osteoblastic differentiation, thus suggesting that these materials may possess a higher potency to enhance osteogenesis than TCP. KW - Calcium phosphate ceramics KW - Human bone-derived cells KW - Cell-biomaterial interactions KW - Bone substitutes KW - In situ hybridization PY - 2004 DO - https://doi.org/10.1016/S0142-9612(03)00525-8 SN - 0142-9612 VL - 25 IS - 2 SP - 335 EP - 344 PB - Elsevier CY - Oxford AN - OPUS4-2957 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Knabe, Ch. A1 - Berger, Georg A1 - Gildenhaar, Renate A1 - Klar, F. A1 - Zreiqat, H. T1 - The modulation of osteogenesis in vitro by calcium titanium phosphate coatings JF - Biomaterials N2 - Calcium phosphate coated titanium and titanium alloy are widely used as dental and orthopaedic implants. This study examines the effect of novel calcium titanium and calcium titanium zirconium phosphates suitable for plasma-spraying onto titanium substrata on the expression of bone-related genes and proteins by human bone-derived cells (HBDC) and compares this behavior to that on native titanium and hydroxyapatite-coated titanium. Test materials were an acid etched and sand-blasted titanium surface (Ti-DPS), a plasma-sprayed hydroxyapatite coating (HA), and five materials which were created from CaTi4(PO4)6 (CTP) and CaZr4(PO4)6 (CZP): sintered CaTi4(PO4)6 (CTP-S1), sintered 46CaO·23TiO2·31P2O5 (CTP-S2), sintered CaTiZr3(PO4)6, (CTZP-S1), sintered 46CaO·23ZrO2·31P2O5 (CTZP-S2) and sintered 55CaO·20TiO2·31P2O5 (CTP-S3). HBDC were grown on the substrata for 3, 7, 14 and 21 d, counted and probed for various mRNAs and proteins (type I collagen, osteocalcin, osteopontin, osteonectin, alkaline phosphatase and bone sialoprotein). All substrates significantly affected cellular growth and the temporal expression of an array of bone-related genes and proteins. At 14 and 21 d, cells on CTP-S3 displayed significantly enhanced expression of all osteogenic mRNAs. Surfaces of CTP-S1 and CTP-S3 had the most effect on osteoblastic differentiation inducing a greater expression of an array of osteogenic markers than recorded for cells grown on Ti-DPS and HA, suggesting that these novel materials may possess a higher potency to enhance osteogenesis. KW - Calcium phosphate coatings KW - Osteoblast KW - In vitro test KW - In situ hybridization KW - Osteogenesis PY - 2004 DO - https://doi.org/10.1016/j.biomaterials.2004.01.059 SN - 0142-9612 VL - 25 IS - 20 SP - 4911 EP - 4919 PB - Elsevier CY - Oxford AN - OPUS4-3501 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Zreiqat, H. A1 - Berger, Georg A1 - Gildenhaar, Renate A1 - Knabe, Ch. T1 - The Functional Expression of Human Bone-Derived Cells Grown on Rapidly Resorbable Calcium Phosphate Ceramics JF - Key engineering materials KW - Calcium phosphate ceramics KW - Human bone-derived cells KW - Cell-biomaterial interactions KW - Bone-substitutes KW - in situ hybridization PY - 2004 UR - http://www.scientific.net/1013-9826/ SN - 1013-9826 VL - 254-256 SP - 1059 EP - 1062 PB - Trans Tech Publ. CY - Aedermannsdorf AN - OPUS4-2750 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Österle, Werner A1 - Klaffke, Dieter A1 - Griepentrog, Michael A1 - Gross, U. A1 - Kranz, I. A1 - Knabe, Ch. T1 - Potential of wear resistant coatings on Ti-6Al-4V for artificial hip joint bearing surfaces JF - Wear N2 - Tribological screening tests (simple, reciprocating ball-on-flat tests) were performed with the objective to identify an appropriate coating for the articulating surfaces of artificial hip joints whose acetabular cups and femoral stems are made from Ti–6Al–4V alloy, which is appreciated for its light weight, good biocompatibility and elastic properties similar to those of natural bone. Standard coatings like TiN or CrN performed better than more complicated multi-layer systems, though not as good as different types of amorphous carbon coatings, generally referred to as diamond-like carbon or DLC coatings. Among the latter, hydrogenated amorphous carbon (a-C:H) displayed the best properties, especially if the hydrogen content was increased by reducing the bias voltage during PA-CVD-deposition. The optimised a-C:H coating revealed the most promising wear behaviour under the applied testing conditions, i.e. the increase of linear wear with the number of cycles was close to zero. Regarding the materials examined in this study, correlation of wear with mechanical properties obtained by nano-indentation revealed that high hardness was not an adequate criterion for selecting appropriate coatings. A high ratio of hardness and elastic modulus (H/E) proved to be more important. Microstructural and micro-analytical investigations revealed transformation of TiN and CrN to TiO2 and Cr2O3, respectively, and amorphous carbon was, at least partly, transformed to graphite. Furthermore, incorporation of Al2O3 from the ball was observed at a very fine scale. The wear debris of favourable coatings always formed agglomerates of nano-scale particles. It was shown that commercial nano-particles of Al2O3, Cr2O3 and carbon black are comparable to particles generated by the tribological tests. However, it is uncertain whether they are comparable to those formed during simulator studies or in vivo. Furthermore, the performance of the favourable coating has to be tested in a hip joint simulator before its potential for application in prostheses can be assessed. KW - Coating KW - Implant KW - Titanium alloy KW - DLC KW - Wear debris KW - Nano-particles PY - 2008 DO - https://doi.org/10.1016/j.wear.2007.04.001 SN - 0043-1648 VL - 264 IS - 7-8 SP - 505 EP - 517 PB - Elsevier CY - Amsterdam AN - OPUS4-16711 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Knabe, Ch. A1 - Howlett, CR. A1 - Markovic, B. A1 - Gildenhaar, Renate A1 - Berger, Georg A1 - Zreiqat, H. T1 - Beeinflussung der Gen- und Proteinexpression von Osteoblasten durch bioaktive resorbierbare Glaskeramiken T2 - Gemeinschaftstagung der Deutschen Ges. f. Implantologie im Zahn-, Mund-, und Kieferbereich, der Österreichischen Ges. f. Orale Chirurgie und Implantologie und der Schweizerischen Ges. f. Orale Implantologie T2 - Gemeinschaftstagung der Deutschen Ges. f. Implantologie im Zahn-, Mund-, und Kieferbereich, der Österreichischen Ges. f. Orale Chirurgie und Implantologie und der Schweizerischen Ges. f. Orale Implantologie CY - Munich, Germany DA - 2002-12-04 PY - 2002 AN - OPUS4-6201 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Knabe, Ch. A1 - Howlett, CR. A1 - Markovic, B. A1 - Gildenhaar, Renate A1 - Berger, Georg A1 - Zreiqat, H. T1 - The fuctional expression of human osteoblasts grown on resorbable calcium phosphates T2 - 15th International Symposium on Ceramics in Medicine (Bioceramics 15) T2 - 15th International Symposium on Ceramics in Medicine (Bioceramics 15) CY - Sydney, Australia DA - 2002-12-04 PY - 2002 AN - OPUS4-6202 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Knabe, Ch. A1 - Howlett, CR. A1 - Markovic, B. A1 - Gildenhaar, Renate A1 - Berger, Georg A1 - Reif, D. A1 - Zreiqat, H. T1 - The fuctional expression of human bone derived cells grown on resorbable bioactive glass ceramics T2 - 28th Annuall Meeting & Explosion of the Society for Biomaterials T2 - 28th Annuall Meeting & Explosion of the Society for Biomaterials CY - Tampa, FL, USA DA - 2002-04-23 PY - 2002 AN - OPUS4-6203 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Knabe, Ch. A1 - Berger, Georg A1 - Gildenhaar, Renate T1 - Effect of resorbable calcium-alkali-phosphate bone substitute materials one bone formation and osteoblastic phenotype expression in vivo T2 - 8th World Biomaterials Congress T2 - 8th World Biomaterials Congress CY - Amsterdam, Netherlands DA - 2008-05-28 PY - 2008 AN - OPUS4-16198 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Knabe, Ch. A1 - Berger, Georg A1 - Gildenhaar, Renate A1 - Houshmand, A. A1 - Müller-Mai, Ch. A1 - Jörn, Daniela A1 - Stiller, M. T1 - Effect of resorbable calcium-alkali-orthophosphate bone substitute cements on osteogenesis after implantation in the rabbit femur T2 - Transaction 36th Annual Meeting of the Soc. for Biomaterials USA T2 - Transaction 36th Annual Meeting of the Soc. for Biomaterials USA CY - Seattle, WA, USA DA - 2010-04-21 PY - 2010 AN - OPUS4-22515 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -