TY - JOUR A1 - Montero-Calasanz, M.C. A1 - Göker, M. A1 - Pötter, G. A1 - Rohde, M. A1 - Spröer, C. A1 - Schumann, P. A1 - Gorbushina, Anna A1 - Klenk, H.P. T1 - Geodermatophilus saharensis sp. nov., isolated from sand of the Saharan desert in Chad N2 - A novel Gram-positive, aerobic, actinobacterial strain, CF5/5, was isolated from soil in the Sahara desert, Chad. It grew best at 20–35 °C and at pH 6.0–8.0 and with 0–4 % (w/v) NaCl, forming black-colored colonies. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The DNA G + C content was 75.9 mol%. The peptidoglycan contained meso-diaminopimelic acid; galactose and xylose were detected as diagnostic sugars. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, and phosphatidylinositol; MK-9(H4) was the dominant menaquinone. The major cellular fatty acids were: iso-C16:0 and iso-C15:0. The 16S rRNA gene showed 95.6–98.3 % sequence similarity with the other named members of the genus Geodermatophilus. Based on the polyphasic taxonomy data, the isolate is proposed to represent a novel species, Geodermatophilus saharensis with the type strain CF5/5T = DSM 45423 = CCUG 62813 = MTCC 11416. KW - Actinomycetes KW - Geodermatophilaceae KW - Taxonomy KW - Sahara desert KW - Phenotype microarray PY - 2012 U6 - https://doi.org/10.1007/s00203-012-0860-8 SN - 0302-8933 SN - 1432-072X IS - Dezember SP - 1 EP - 7(?) PB - Springer CY - Berlin ; Heidelberg AN - OPUS4-27630 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Montero-Calasanz, M.C. A1 - Göker, M. A1 - Pötter, G. A1 - Rohde, M. A1 - Spröer, C. A1 - Schumann, P. A1 - Gorbushina, Anna A1 - Klenk, H.-P. T1 - Geodermatophilus arenarius sp. nov., a xerophilic actinomycete isolated from Saharan desert sand in Chad N2 - A novel Gram-positive, aerobic, actinobacterial strain, CF5/4T, was isolated in 2007 during an environmental screening of arid desert soil in Ouré Cassoni, Chad. The isolate grew best in a temperature range of 28–40 °C and at pH 6.0-8.5, with 0-1 % (w/v) NaCl, forming brown-coloured and nearly circular colonies on GYM agar. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The DNA G + C content of the novel strain was 75.9 mol %. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diaminoacid. The main phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, diphosphatidylglycerol and a small amount of phosphatidylglycerol; MK-9(H4) was identified as the dominant menaquinone and galactose as diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids: iso-C15:0 and iso-C16:0. The 16S rRNA gene showed 96.2–98.3 % sequence identity with the three members of the genus Geodermatophilus: G. obscurus (96.2 %), G. ruber (96.5 %), and G. nigrescens (98.3 %). Based on the chemotaxonomic results, 16S rRNA gene sequence analysis and DNA–DNA hybridization with the type strain of G. nigrescens, the isolate is proposed to represent a novel species, Geodermatophilus arenarius (type strain CF5/4T = DSM 45418T = MTCC 11413T = CCUG 62763T). KW - Xerophiles KW - Actinomycetes KW - Geodermatophilaceae KW - Taxonomy KW - Sahara desert PY - 2012 U6 - https://doi.org/10.1007/s00792-012-0486-4 SN - 1431-0651 SN - 1433-4909 VL - 16 IS - 6 SP - 903 EP - 909 PB - Springer CY - Tokyo AN - OPUS4-26933 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -