TY - JOUR A1 - Montero-Calasanz, M.d.C. A1 - Göker, M. A1 - Pötter, G. A1 - Rohde, M. A1 - Spröer, C. A1 - Schumann, P. A1 - Gorbushina, Anna A1 - Klenk, H.-P. T1 - Geodermatophilus africanus sp. nov., a halotolerant actinomycete isolated from Saharan desert sand N2 - A novel Gram-strain positive, aerobic, actinobacterial strain, designated CF11/1T, was isolated from a sand sample obtained in the Sahara Desert, Chad. The black-pigmented isolate was aerobic and exhibited optimal growth from 25 to 35 °C at pH 6.0–8.0 and with 0–8 % (w/v) NaCl, indicating that it is a halotolerant mesophile. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The G+C content in the genome was 74.4 mol%. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diaminoacid. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and a minor fraction of phosphatidylglycerol; MK-9(H4) was the dominant menaquinone, and galactose was detected as a diagnostic sugar. The major cellular fatty acid was branched-chain saturated acid iso-C16:0. Analysis of 16S rRNA gene sequences showed 95.3–98.6 % pairwise sequence identity with the members of the genus Geodermatophilus. Based on phenotypic and chemotaxonomic properties, as well as phylogenetic distinctiveness, the isolate represents a novel species, Geodermatophilus africanus, with the type strain CF11/1T (DSM 45422 = CCUG 62969 = MTCC 11556). KW - Actinomycetes KW - Geodermatophilaceae KW - Taxonomy KW - Osmotolerant KW - Phenotype microarray PY - 2013 U6 - https://doi.org/10.1007/s10482-013-9939-8 SN - 0003-6072 SN - 1572-9699 VL - 104 IS - 2 SP - 207 EP - 216 PB - Springer CY - Dordrecht AN - OPUS4-28868 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Montero-Calasanz, M.C. A1 - Göker, M. A1 - Pötter, G. A1 - Rohde, M. A1 - Spröer, C. A1 - Schumann, P. A1 - Gorbushina, Anna A1 - Klenk, H.P. T1 - Geodermatophilus saharensis sp. nov., isolated from sand of the Saharan desert in Chad N2 - A novel Gram-positive, aerobic, actinobacterial strain, CF5/5, was isolated from soil in the Sahara desert, Chad. It grew best at 20–35 °C and at pH 6.0–8.0 and with 0–4 % (w/v) NaCl, forming black-colored colonies. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The DNA G + C content was 75.9 mol%. The peptidoglycan contained meso-diaminopimelic acid; galactose and xylose were detected as diagnostic sugars. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, and phosphatidylinositol; MK-9(H4) was the dominant menaquinone. The major cellular fatty acids were: iso-C16:0 and iso-C15:0. The 16S rRNA gene showed 95.6–98.3 % sequence similarity with the other named members of the genus Geodermatophilus. Based on the polyphasic taxonomy data, the isolate is proposed to represent a novel species, Geodermatophilus saharensis with the type strain CF5/5T = DSM 45423 = CCUG 62813 = MTCC 11416. KW - Actinomycetes KW - Geodermatophilaceae KW - Taxonomy KW - Sahara desert KW - Phenotype microarray PY - 2012 U6 - https://doi.org/10.1007/s00203-012-0860-8 SN - 0302-8933 SN - 1432-072X IS - Dezember SP - 1 EP - 7(?) PB - Springer CY - Berlin ; Heidelberg AN - OPUS4-27630 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Del Carmen Montero-Calasanz, M. A1 - Göker, M. A1 - Rohde, M. A1 - Schumann, P. A1 - Pötter, G. A1 - Spröer, C. A1 - Gorbushina, Anna A1 - Klenk, H.-P. T1 - Geodermatophilus siccatus sp. nov., isolated from arid sand of the Saharan desert in Chad N2 - A novel Gram-positive, aerobic, actinobacterial strain, CF6/1T, was isolated in 2007 during environmental screening of arid desert soil in the Sahara near to Ourba, Chad. The isolate was found to grow best in a temperature range of 20–37 °C and at pH 6.0–8.5 and showed no NaCl tolerance, forming black-coloured and nearly circular colonies on GYM agar. Chemotaxonomic and molecular characteristics determined for the isolate match those previously described for members of the genus Geodermatophilus. The DNA G + C content of the novel strain was determined to be 74.9 mol %. The peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid. The main phospholipids were determined to be phosphatidylethanolamine, phosphatidylinositol, phosphatidylcholine, diphosphatidylglycerol and traces of phosphatidylglycerol; MK-9(H4) was identified as the dominant menaquinone and galactose as the diagnostic sugar. The major cellular fatty acids were found to be the branched-chain saturated acids iso-C16:0 and iso-C15:0, as well as C17:1ω8c. The 16S rRNA gene sequence shows 97.5–97.9 % sequence identity with the four validly named or at least effectively published members of the genus: Geodermatophilus obscurus (97.5 %), Geodermatophilus arenarius (97.7 %), Geodermatophilus ruber (97.9 %) and Geodermatophilus nigrescens (97.9 %). Based on the results from this polyphasic taxonomic analysis and DNA–DNA hybridizations with all type strains of the genus, we propose that strain CF6/1T represents a novel species, Geodermatophilus siccatus, with the type strain CF6/1T = DSM 45419T = CCUG 62765T = MTCC 11414T. KW - Actinomycetes KW - Geodermatophilaceae KW - Taxonomy KW - Xerophiles KW - Phenotype microarray PY - 2013 U6 - https://doi.org/10.1007/s10482-012-9824-x SN - 0003-6072 SN - 1572-9699 VL - 103 IS - 3 SP - 449 EP - 456 PB - Springer CY - Dordrecht AN - OPUS4-28082 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -