TY - JOUR A1 - Küttenbaum, Stefan A1 - Braml, T. A1 - Heinze, M. A1 - Kainz, C. A1 - Keuser, M. A1 - Kotz, P. A1 - Lechner, T. A1 - Maack, Stefan A1 - Reinke, K.-D. A1 - Schulze, S. A1 - Soukup, A. A1 - Stettner, C. A1 - Taffe, A. A1 - Wöstmann, Jens T1 - Guideline on NDT-supported reliability assessment of existing structures - Current developments in Germany N2 - Information about an existing structure can be collected at certain costs to evaluate the reliability and condition as realistically as necessary. This information can be relevant or irrelevant, true or biased, precise or imprecise. The incorporation of relevant and quality-assessed measured information into reliability reassessment offers the chance to extend remaining lifetimes and support decision making about optimal actions or maintenance strategies. This paper shows recent developments in a national research project that aims to produce a guideline on the NDT-based, structure-specific modification of partial safety factors. The general methodology, results from recalculations according to the Eurocodes and metrologically solvable testing tasks relevant in the recalculation of the concrete bridges are shown and compared with the non-destructive testing methods applicable to concrete bridges. A case study is used to demonstrate that as-built drawings, in this case of the positions of tendons and shear reinforcement, can be verified using the radar method. KW - Non-destructive testing KW - Concrete bridges KW - Partial safety factor modification PY - 2023 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-584862 DO - https://doi.org/10.1002/cepa.2168 SN - 2509-7075 VL - 6 IS - 5 SP - 537 EP - 543 PB - Ernst & Sohn CY - Berlin AN - OPUS4-58486 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Smeir, E. A1 - Leberer, S. A1 - Blumrich, A. A1 - Vogler, G. A1 - Vasiliades, A. A1 - Dresen, S. A1 - Jaeger, Carsten A1 - Gloaguen, Y. A1 - Klose, C. A1 - Beule, D. A1 - Schulze, P. A1 - Bodmer, R. A1 - Foryst-Ludwig, A. A1 - Kintscher, U. T1 - Depletion of Cardiac Cardiolipin Synthase Alters Systolic and Diastolic Function N2 - Cardiolipin (CL) is a major cardiac mitochondrial phospholipid maintaining regular mitochondrial morphology and function in cardiomyocytes. Cardiac CL production includes ist biosynthesis and a CL-remodeling process. Here we studied the impact of CL-biosynthesis and the enzyme Cardiolipin Synthase (CLS) on cardiac function. CLS and cardiac CL-species were significantly downregulated in cardiomyocytes following catecholamine-induced cardiac damage in mice, accompanied by increased oxygen consumption rates, signs of oxidative stress and mitochondrial uncoupling. RNAi-mediated cardiomyocyte-specific knockdown of CLS in Drosophila melanogaster resulted in marked cardiac dilatation, severe impairment of systolic performance and slower diastolic filling velocity assessed by fluorescence-based heart imaging. Finally, we showed that CL72:8 is significantly decreased in cardiac samples from patients with heart failure with reduced ejection fraction (HFrEF). In summary, we identified CLS as a regulator of cardiac function. Considering the cardiac depletion of CL-species in HFrEF, pharmacological targeting of CLS may be a promising therapeutic approach.zeige mehrzeige weniger KW - High-resolution mass spectrometry KW - Nontarget analysis KW - Heart failure KW - Cardiolipins KW - Lipidomics PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-536833 DO - https://doi.org/10.1016/j.isci.2021.103314 VL - 24 IS - 11 SP - 103314 PB - Cell Press AN - OPUS4-53683 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Feistkorn, S. A1 - Algernon, D. A1 - Arndt, R. A1 - Ebell, Gino A1 - Friese, M. A1 - Grosse, C. U. A1 - Holstein, R. A1 - Niederleithiger, Ernst A1 - Schickert, M. A1 - Schulze, S. A1 - Taffe, A. A1 - Walther, A. A1 - Wolf, J. A1 - Zoega, A. A1 - Zwanzig, M. T1 - Introduction to DIN 4871: Qualification of NDT Personnel in Civil Engineering (NDT‐CE) N2 - Recently, non-destructive testing in civil engineering (NDT-CE), in particular of concrete components, has successfully mastered the leap from research to practice. Several methods have been established for field inspections to determine the concrete cover of reinforcement or to estimate the compressive strength as well as other parameters related to the concrete material. In addition, the application of non-destructive testing is indispensable, if information about the inner structure - such as the location of rebars and tendon ducts or the damage-related condition assessment to detect grouting defects, honeycombs, delamination, or corrosion - is required. Besides the selection of a suitable NDT method and an appropriate inspection system, the reliability of the results depends largely on the person who applies the non-destructive inspection technique and evaluates the inspection results. To ensure a high quality of non-destructive concrete evaluation as well as to keep the uncertainty caused by the inspection personnel to a minimum, structured, consistent, and regulated theoretical as well as practical training of inspection personnel is essential. To close this gap, the subcommittee of education and training (UA-A) within the committee for NDT-CE of the German Society for Non-Destructive Testing (DGZfP) has been reactivated in 2018 to establish uniform training standards for non-destructive concrete inspections in the long term. The subcommittee consists of scientists, practitioners, authorities, and clients. So far, the national standard DIN 4871 “Non-destructive testing - Qualification of NDT personnel in Civil Engineering (NDT-CE)” was developed. This standard considers the civil-industry-specifics, for example, that standards for NDT of concrete, as well as related product standards with a few exceptions, still do not exist at the moment. Within this presentation, the concept, the connection to ISO 9712 and other standards as well as an overview of the recently developed German standard DIN 4871 will be presented. T2 - NDT-CE 2022 CY - Zurich, Switzerland DA - 16.08.2022 KW - Non-destrucive testing KW - Civil engineering PY - 2022 AN - OPUS4-55526 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schulze-Makuch, D. A1 - Haque, S. A1 - Beckles, D. A1 - Schmitt-Kopplin, P. A1 - Harir, M. A1 - Schneider, Beate A1 - Stumpp, C. A1 - Wagner, D. T1 - A chemical and microbial characterization of selected mud volcanoes in Trinidad reveals pathogens introduced by surface water and rain water N2 - Terrestrial mud volcanoes are unique structures driven by tectonic pressure and fluids from the deep subsurface. These structures are mainly found in active tectonic zones, such as the area near the Los Bajos Fault in Trinidad. Here we report a chemical and microbiological characterization of three mud volcanoes, which included analyses of multiple liquid and solid samples from the mud volcanoes. Our study confirms previous suggestions that at least some of the mud volcano fluids are a mixture of deeper salt-rich water and surficial/precipitation water. No apparent water quality differences were found between sampling sites north and south of a major geological fault line. Microbiological analyses revealed diverse communities, both aerobic and anaerobic, including sulfate reducers, methanogens, carbon dioxide fixing and denitrifying bacteria. Several identified species were halophilic and likely derived from the deeper salt-rich subsurface water, while we also cultivated pathogenic species from the Vibrionaceae, Enterobacteriaceae, Shewanellaceae, and Clostridiaceae. These microorganisms were likely introduced into the mud volcano fluids both from surface water or shallow ground-water, and perhaps to a more minor degree by rain water. The identified pathogens are a major health concern that needs to be addressed. KW - Water stable isotope analysis KW - Mud volcanoe fluids KW - Metabolomics PY - 2020 DO - https://doi.org/10.1016/j.scitotenv.2019.136087 VL - 707 SP - 136087 PB - Elsevier B.V. AN - OPUS4-50499 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Brauckmann, C. A1 - Pramann, A. A1 - Rienitz, O. A1 - Schulze, A. A1 - Phukphatthanachai, Pranee A1 - Vogl, Jochen T1 - Combining Isotope Dilution and Standard Addition—Elemental Analysis in Complex Samples N2 - A new method combining isotope dilution mass spectrometry (IDMS) and standard addition has been developed to determine the mass fractions w of different elements in complex matrices: (a) silicon in aqueous tetramethylammonium hydroxide (TMAH), (b) sulfur in biodiesel fuel, and (c) iron bound to transferrin in human serum. All measurements were carried out using inductively coupled plasma mass spectrometry (ICP–MS). The method requires the gravimetric preparation of several blends (bi)—each consisting of roughly the same masses (mx,i) of the sample solution (x) and my,i of a spike solution (y) plus different masses (mz,i) of a reference solution (z). Only these masses and the isotope ratios (Rb,i) in the blends and reference and spike solutions have to be measured. The derivation of the underlying equations based on linear regression is presented and compared to a related concept reported by Pagliano and Meija. The uncertainties achievable, e.g., in the case of the Si blank in extremely pure TMAH of urel (w(Si)) = 90% (linear regression method, this work) and urel (w(Si)) = 150% (the method reported by Pagliano and Meija) seem to suggest better applicability of the new method in practical use due to the higher robustness of regression analysis. KW - Isotope dilution mass spectrometry KW - tandard addition KW - ICP-MS KW - lank characterization KW - Silicon KW - Sulfur KW - Transferrin KW - Tetramethylammonium hydroxide KW - Biodiesel fuel KW - Human serum PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-526376 DO - https://doi.org/10.3390/molecules26092649 VL - 26 IS - 9 SP - 2649 PB - MDPI CY - Basel AN - OPUS4-52637 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Brauckmann, C. A1 - Pramann, A. A1 - Rienitz, O. A1 - Schulze, A. A1 - Phukphatthanachai, P. A1 - Vogl, Jochen T1 - Combining Isotope Dilution and Standard Addition - Elemental Analysis in Complex Samples N2 - A new method combining isotope dilution mass spectrometry (IDMS) and standard addition has been developed to determine the mass fractions w of different elements in complex matrices: (a) silicon in aqueous tetramethylammonium hydroxide (TMAH), (b) sulfur in biodiesel fuel, and (c) iron bound to transferrin in human serum. All measurements were carried out using inductively coupled plasma mass spectrometry (ICP–MS). The method requires the gravimetric preparation of several blends (bi)—each consisting of roughly the same masses (mx,i) of the sample solution (x) and my,i of a spike solution (y) plus different masses (mz,i) of a reference solution (z). Only these masses and the isotope ratios (Rb,i) in the blends and reference and spike solutions have to be measured. The derivation of the underlying equations based on linear regression is presented and compared to a related concept reported by Pagliano and Meija. The uncertainties achievable, e.g., in the case of the Si blank in extremely pure TMAH of urel (w(Si)) = 90% (linear regression method, this work) and urel (w(Si)) = 150% (the method reported by Pagliano and Meija) seem to suggest better applicability of the new method in practical use due to the higher robustness of regression analysis. T2 - CITAC Best Paper Award Ceremony CY - Online meeting DA - 21.06.2022 KW - Isotope dilution mass spectrometry KW - Standard addition KW - ICP-MS KW - Blank characterization KW - Silicon KW - Sulfur KW - Transferrin KW - Tetramethylammonium hydroxide KW - Biodiesel fuel KW - Human serum PY - 2022 AN - OPUS4-55032 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Feistkorn, S. A1 - Algernon, D. A1 - Arndt, R. A1 - Ebell, Gino A1 - Friese, M. A1 - Grosse, C. U. A1 - Holstein, R. A1 - Niederleithinger, Ernst A1 - Schickert, M. A1 - Schulze, S. A1 - Taffe, A. A1 - Walther, A. A1 - Wolf, J. A1 - Zoega, A. A1 - Zwanzig, M. T1 - Introduction to DIN 4871: Qualification of NDT Personnel in Civil Engineering (NDT-CE) N2 - Recently, non-destructive testing in civil engineering (NDT-CE), in particular of concrete components, has successfully mastered the leap from research to practice. Several methods have been established for field inspections to determine the concrete cover of reinforcement or to estimate the compressive strength as well as other parameters related to the concrete material. In addition, the application of nondestructive testing is indispensable, if information about the inner structure - such as the location of rebars and tendon ducts or the damage-related condition assessment to detect grouting defects, honeycombs, delamination, or corrosion - is required. Besides the selection of a suitable NDT method and an appropriate inspection system, the reliability of the results depends largely on the person who applies the non-destructive inspection technique and evaluates the inspection results. To ensure a high quality of non-destructive concrete evaluation as well as to keep the uncertainty caused by the inspection personnel to a minimum, structured, consistent and regulated theoretical as well as practical training of inspection personnel is essential. To close this gap, the subcommittee of education (UA-A) within the committee for NDT-CE of the German Society for Nondestructive Testing (DGZfP) has been reactivated in 2018 to establish uniform training standards for nondestructive concrete inspections in the long term. The subcommittee consists of scientists, practitioners, authorities, and clients. So far, the national standard DIN 4871 “Non-destructive testing - Qualification and Certification of NDT personnel in Civil Engineering (NDT-CE)” was developed and is currently under review. This standard considers the civil-industry-specifics, for example, that standards for NDT of concrete, as well as related product standards with a few exceptions, still do not exist at the moment. Within this presentation, the concept, the connection to ISO 9712 and other standards as well as an overview of the developed German standard DIN 4871 will be presented. T2 - NDT-CE 2022 CY - Zurich, Switzerland DA - 16.08.2022 KW - Non-destrucive testing KW - Civil engineering PY - 2022 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-555250 UR - https://www.ndt.net/article/ndtce2022/paper/66594_manuscript.pdf VL - 2022/09 SP - 1 EP - 12 PB - NDT.net CY - Bad Breisig AN - OPUS4-55525 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Weigel, Sandra A1 - Sielaff, S. A1 - Kunsic-Nowak, T. A1 - Nytus, N. A1 - Schwettmann, K. A1 - Schulze, C. A1 - Wetekam, J. T1 - Die FTIR-Untersuchung von Bitumen: Chancen, Durchführung und Präzision N2 - Die Fourier-Transformations-Infrarot-Spektroskopie (FTIR-Spektroskopie) stellt eine innovative und wirkungsvolle Methode zur Charakterisierung von Bitumen und bitumenhaltigen Bindemitteln dar. So erlaubt die FTIRCharakterisierung nicht nur eine Bewertung des Alterungsfortschritts eines Bindemittels, sondern zudem auch die Identifizierung von im Bitumen enthaltenen Additiven und Schadstoffen wie z. B. polyzyklischen aromatischen Kohlenwasserstoffen (PAK), die Ableitung verschiedener physikalischer und chemischer Kennwerte sowie weiterhin eine Klassifizierung von Bindemitteln nach verschiedenen übergeordneten Eigenschaften wie der Raffinerie, dem Alterungszustand und auch der Alterungsempfindlichkeit. KW - Bitumen und bitumenhaltige Bindemittel KW - FTIR-Spektroskopie KW - Anwendungsmöglichkeiten KW - Vergleichuntersuchung KW - Arbeitsanleitung KW - Präzision PY - 2023 UR - https://www.kirschbaum.de/fachzeitschriften/strasse-und-autobahn/strasse-und-autobahn/aktuelles-heft-17.html DO - https://doi.org/10.53184/STA8-2023-4 VL - 74/8 SP - 630 EP - 642 PB - KIRSCHBAUM VERLAG GmbH CY - Bonn AN - OPUS4-59049 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Keller, S. A1 - Borde, T. A1 - Brangsch, J. A1 - Reimann, C. A1 - Kader, A. A1 - Schulze, D. A1 - Buchholz, R. A1 - Kaufmann, Jan Ole A1 - Karst, U. A1 - Schellenberger, E. A1 - Hamm, B. A1 - Makowski, M. R. T1 - Assessment of the hepatic tumor extracellular matrix using elastin‑specific molecular magnetic resonance imaging in an experimental rabbit cancer model N2 - To investigate the imaging performance of an elastin-specific molecular magnetic resonance imaging (MRI) probe with respect to the extracellular matrix (ECM) in an experimental hepatic cancer model. Twelve rabbits with hepatic VX2 tumors were examined using 3 T MRI 14, 21, and 28 days after tumor implantation for two subsequent days (gadobutrol, day 1; elastin-specific probe, day 2). The relative enhancement (RE) of segmented tumor regions (central and margin) and the peritumoral matrix was calculated using pre-contrast and delayed-phase T1w sequences. MRI measurements were correlated to histopathology and element-specific and spatially resolved mass spectrometry (MS). Mixed-model analysis was performed to assess the performance of the elastin-specific probe. In comparison to gadobutrol, the elastin probe showed significantly stronger RE, which was pronounced in the tumor margin (day 14–28: P ≤ 0.007). In addition, the elastin probe was superior in discriminating between tumor regions (χ2(4) = 65.87; P < 0.001). MRI-based measurements of the elastin probe significantly correlated with the ex vivo elastinstain (R = .84; P <0 .001) and absolute gadolinium concentrations (ICP-MS: R = .73, P <0 .01). LA-ICP-MS imaging confirmed the colocalization of the elastin-specific probe with elastic fibers. Elastin-specific molecular MRI is superior to non-specific gadolinium-based contrast agents in imaging the ECM of hepatic tumors and the peritumoral tissue. KW - Elastin-specific molecular agent KW - Extracellular matrix KW - Hepatocellular carcinoma KW - Inductively coupled plasma mass spectroscopy KW - Laser ablation-inductively coupled plasma-mass spectrometry KW - Magnetic resonance imaging KW - MR imaging KW - ESMA KW - Gadolinium PY - 2020 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-517360 DO - https://doi.org/10.1038/s41598-020-77624-8 VL - 10 IS - 1 SP - 20785 PB - Nature AN - OPUS4-51736 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -