TY - CONF A1 - Matschulat, Andrea T1 - New hybrid probes for NIR-SERS sensing T2 - FTIR-Workshop, Robert-Koch-Institut CY - Berlin, Germany DA - 2009-10-15 PY - 2009 AN - OPUS4-19942 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Matschulat, Andrea T1 - SERS hybrid probes as versatile tools for biological sensing T2 - Symposium Licht-Materialien-Modelle CY - Berlin, Germany DA - 2009-09-07 PY - 2009 AN - OPUS4-19953 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Matschulat, Andrea T1 - Multivariate analysis for surface-enhanced Raman scatterin (SERS) probe multiplexing and imaging in biological matrics T2 - DPG-Frühjahrstagung Sektion: Kondensierte Materie CY - Regensburg, Germany DA - 2010-03-21 PY - 2010 AN - OPUS4-21581 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Matschulat, Andrea T1 - NIR-SERS probes for in vitro and in vivo bioanalytics T2 - GdCH - Doktorandentagung CY - Attendorn, Germany DA - 2009-02-15 PY - 2009 AN - OPUS4-19198 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Matschulat, Andrea T1 - NIR SERS hybrid probes for vitro and in vivo bioanalytics T2 - DGD - Frühjahrstagung CY - Dresden, Germany DA - 2009-03-22 PY - 2009 AN - OPUS4-19199 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Matschulat, Andrea T1 - NIR-SERS probes for in vitro and in vivo bioanalytics T2 - ANAKON CY - Berlin, Germany DA - 2009-03-17 PY - 2009 AN - OPUS4-19209 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Matschulat, Andrea A1 - Drescher, Daniela A1 - Kneipp, Janina T1 - Surface-enhanced Raman scattering hybrid nanoprobe multiplexing and imaging in biological systems N2 - Surface-enhanced Raman scattering (SERS) labels and probes consisting of gold and silver nanoaggregates and attached reporter molecules can be identified by the Raman signature of the reporter molecule. At the same time, SERS hybrid probes deliver sensitive molecular structural information on their nanoenvironment. Here we demonstrate full exploitation of the multifunctional and multiplexing capabilities inherent to such nanoprobes by applying cluster methods and principal components approaches for discrimination beyond the visual inspection of individual spectra that has been practiced so far. The reported results indicate that fast, multivariate evaluation of whole sets of multiple probes is feasible. Spectra of five different reporters were shown to be separable by hierarchical clustering and by principal components analysis (PCA). In a duplex imaging approach in live cells, hierarchical cluster analysis, K-means clustering, and PCA were used for imaging the positions of different types of SERS probes along with the spectral information from cellular constituents. Parallel to cellular imaging experiments, cytotoxicity of the SERS hybrid probes containing aromatic thiols as reporters is assessed. The reported results suggest multiplexing applications of the nontoxic SERS nanoprobes in high density sensing and imaging in complex biological structures. KW - Surface-enhanced Raman scattering KW - Nanosensor KW - Para-aminobenzenethiol KW - 2-naphthalenethiol KW - 3T3 cells KW - Principal component analysis KW - Hierarchical cluster analysis KW - Cytotoxicity KW - Imaging PY - 2010 DO - https://doi.org/10.1021/nn100280z SN - 1936-0851 VL - 4 IS - 6 SP - 3259 EP - 3269 PB - ACS Publ. CY - Washington, DC, USA AN - OPUS4-23212 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Matschulat, Andrea T1 - NiR-SERS probes for in vitro and in vivo bioanalytics T2 - Tag der Chemie CY - Potsdam, Germany DA - 2009-06-04 PY - 2009 AN - OPUS4-19301 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Joseph, Viginia A1 - Matschulat, Andrea A1 - Polte, Jörg A1 - Rolf, Simone A1 - Emmerling, Franziska A1 - Kneipp, Janina T1 - SERS enhancement of gold nanospheres of defined size N2 - Monodisperse, citrate-stabilized gold nanoparticles of sizes ranging from 15 to 40 nm were synthesized and characterized by small angle X-ray scattering and UV-vis experiments. Identical surface properties of nanoparticles of different sizes to avoid variation in the chemical surface-enhanced Raman scattering (SERS) enhancement, as well as selection of experimental conditions so that no aggregation took place, enabled the investigation of enhancement of individual nanospheres. Enhancement factors (EFs) for SERS were determined using the dye crystal violet (CV). EFs for individual gold nanospheres ranged from 102 to 103, in agreement with theoretical predictions. An increase of the EFs of individual spheres with size can be correlated to changes in the extinction spectra of nanoparticle solutions. This confirms that the increase in enhancement with increasing size results from an increase in electromagnetic enhancement. Beyond this dependence of EFs of isolated gold spheres on their size, EFs were shown to vary with analyte concentration as a result of analyte-induced aggregation. This has implications for the application of nanoparticle solutions as SERS substrates in quantitative analytical tasks. KW - Surface-enhanced Raman scattering KW - Electromagnetic enhancement factor KW - Crystal violet KW - Citrate reduction KW - Gold nanoparticles PY - 2011 DO - https://doi.org/10.1002/jrs.2939 SN - 0377-0486 SN - 1097-4555 VL - 42 IS - 9 SP - 1736 EP - 1742 PB - Wiley CY - Chichester AN - OPUS4-24483 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Matschulat, Andrea T1 - SERS- based optical probes for in vitro and in vivo Bioanalysis T2 - ICORS 2008 CY - London, England DA - 2008-08-17 PY - 2008 AN - OPUS4-17988 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -