TY - JOUR A1 - Graf, P. A1 - Mantion, Alexandre A1 - Foelske, A. A1 - Shkilnyy, A. A1 - Masic, A. A1 - Thünemann, Andreas A1 - Taubert, A. T1 - Peptide-coated silver nanoparticles: Synthesis, surface chemistry, and pH-triggered, reversible assembly into particle assemblies N2 - Simple tripeptides are scaffolds for the synthesis and further assembly of peptide/silver nanoparticle composites. Herein, we further explore peptide-controlled silver nanoparticle assembly processes. Silver nanoparticles with a pH-responsive peptide coating have been synthesized by using a one-step precipitation/coating route. The nature of the peptide/silver interaction and the effect of the peptide on the formation of the silver particles have been studied via UV/Vis, X-ray photoelectron, and surface-enhanced Raman spectroscopies as well as through electron microscopy, small angle X-ray scattering and powder X-ray diffraction with Rietveld refinement. The particles reversibly form aggregates of different sizes in aqueous solution. The state of aggregation can be controlled by the solution pH value. At low pH values, individual particles are present. At neutral pH values, small clusters form and at high pH values, large precipitates are observed. KW - Hybrid materials KW - Nano-particles KW - Oligopeptides KW - pH KW - Silver PY - 2009 DO - https://doi.org/10.1002/chem.200802329 SN - 0947-6539 SN - 1521-3765 VL - 15 IS - 23 SP - 5831 EP - 5844 PB - Wiley-VCH Verl. CY - Weinheim AN - OPUS4-19514 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Haase, A. A1 - Mantion, Alexandre A1 - Graf, P. A1 - Plendl, J. A1 - Thünemann, Andreas A1 - Meier, W. A1 - Taubert, A. A1 - Luch, A. T1 - A novel type of silver nanoparticles and their advantages in toxicity testing in cell culture systems N2 - Silver nanoparticles (SNPs) are among the most commercialized nanoparticles worldwide. Often SNP are used because of their antibacterial properties. Besides that they possess unique optic and catalytic features, making them highly interesting for the creation of novel and advanced functional materials. Despite its widespread use only little data exist in terms of possible adverse effects of SNP on human health. Conventional synthesis routes usually yield products of varying quality and property. It thus may become puzzling to compare biological data from different studies due to the great variety in sizes, coatings or shapes of the particles applied. Here, we applied a novel synthesis approach to obtain SNP of well-defined colloidal and structural properties. Being stabilized by a covalently linked small peptide, these particles are nicely homogenous, with narrow size distribution, and form monodisperse suspensions in aqueous solutions. We applied these peptide-coated SNP in two different sizes of 20 or 40 nm (Ag20Pep and Ag40Pep) and analyzed responses of THP-1-derived human macrophages while being exposed against these particles. Gold nanoparticles of similar size and coating (Au20Pep) were used for comparison. The cytotoxicity of particles was assessed by WST-1 and LDH assays, and the uptake into the cells was confirmed via transmission electron microscopy. In summary, our data demonstrate that this novel type of SNP is well suited to serve as model system for nanoparticles to be tested in toxicological studies in vitro. KW - Silver nanoparticles KW - Peptide coating KW - Nanotoxicity PY - 2012 DO - https://doi.org/10.1007/s00204-012-0836-0 SN - 0340-5761 SN - 1432-0738 VL - 86 IS - 7 SP - 1089 EP - 1098 PB - Springer CY - Berlin ; Heidelberg [u.a.] AN - OPUS4-26269 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Haase, A. A1 - Tentschert, J. A1 - Jungnickel, H. A1 - Graf, P. A1 - Mantion, Alexandre A1 - Draude, F. A1 - Plendl, J. A1 - Goetz, M.E. A1 - Galla, S. A1 - Masic, A. A1 - Thünemann, Andreas A1 - Taubert, A. A1 - Arlinghaus, H. F. A1 - Luch, A. T1 - Toxicity of silver nanoparticles in human macrophages: uptake, intracellular distribution and cellular responses N2 - Silver nanoparticles (SNP) are among the most commercialized nanoparticles worldwide. They can be found in many diverse products, mostly because of their antibacterial properties. Despite its widespread use only little data on possible adverse health effects exist. It is difficult to compare biological data from different studies due to the great variety in sizes, coatings or shapes of the particles. Here, we applied a novel synthesis approach to obtain SNP, which are covalently stabilized by a small peptide. This enables a tight control of both size and shape. We applied these SNP in two different sizes of 20 or 40 nm (Ag20Pep and Ag40Pep) and analyzed responses of THP-1-derived human macrophages. Similar gold nanoparticles with the same coating (Au20Pep) were used for comparison and found to be non-toxic. We assessed the cytotoxicity of particles and confirmed their cellular uptake via transmission electron microscopy and confocal Raman microscopy. Importantly a majority of the SNP could be detected as individual particles spread throughout the cells. Furthermore we studied several types of oxidative stress related responses such as induction of heme oxygenase I or formation of protein carbonyls. In summary, our data demonstrate that even low doses of SNP exerted adverse effects in human macrophages. KW - Silver nanoparticles KW - Neurotoxicology KW - Protein carbonyls KW - ROS PY - 2011 DO - https://doi.org/10.1088/1742-6596/304/1/012030 SN - 1742-6588 SN - 1742-6596 VL - 304 SP - 012030-1 - 012030-14 PB - IOP Publ. CY - Bristol, UK AN - OPUS4-24035 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Tentschert, J. A1 - Draude, F. A1 - Jungnickel, H. A1 - Haase, A. A1 - Mantion, Alexandre A1 - Galla, S. A1 - Thünemann, Andreas A1 - Taubert, A. A1 - Luch, A. A1 - Arlinghaus, H. F. T1 - TOF-SIMS analysis of cell membrane changes in functional impaired human macrophages upon nanosilver treatment N2 - Silver nanoparticles (SNP) are among the most commercialized nanoparticles. Here, we show that peptide-coated SNP cause functional impairment of human macrophages. A dose-dependent inhibition of phagocytosis is observed after nanoparticle treatment, and pretreatment of cells with N-acetyl cysteine (NAC) can counteract the phagocytosis disturbances caused by SNP. Using the surface-sensitive mode of time-of-flight secondary ion mass spectrometry, in combination with multivariate statistical methods, we studied the composition of cell membranes in human macrophages upon exposure to SNP with and without NAC preconditioning. This method revealed characteristic changes in the lipid pattern of the cellular membrane outer leaflet in those cells challenged by SNP. Statistical analyses resulted in 19 characteristic ions, which can be used to distinguish between NAC pretreated and untreated macrophages. The present study discusses the assignments of surface cell membrane phospholipids for the identified ions and the resulting changes in the phospholipid pattern of treated cells. We conclude that the adverse effects in human macrophages caused by SNP can be partially reversed through NAC administration. Some alterations, however, remained. KW - Silver nanoparticles KW - Lipidomics KW - N-acetyl cysteine KW - Phagocytosis KW - Oxidative stress KW - Reference material PY - 2013 DO - https://doi.org/10.1002/sia.5155 SN - 0142-2421 SN - 1096-9918 VL - 45 IS - 1 SP - 483 EP - 485 PB - Wiley CY - Chichester AN - OPUS4-27586 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Nehring, R. A1 - Palivan, C.G. A1 - Moreno-Flores, S. A1 - Mantion, Alexandre A1 - Tanner, P. A1 - Toca-Herrera, J.L. A1 - Thünemann, Andreas A1 - Meier, W. T1 - Protein decorated membranes by specific molecular interactions N2 - Here we characterize new metal-functionalized amphiphilic diblock copolymers, developed for both surface and solution molecular recognition applications. Polybutadiene-block-poly(ethylene oxide) copolymers functionalized with nitrilotriacetic acid and tris(nitrilotriacetic acid) were complexed with nickel(II) to obtain coordination sites for oligohistidine residues of model proteins. Mixtures of functionalized polymers with the respective non-functionalized block copolymers self-assemble in aqueous solution into vesicular structures with a controlled density of the metal end-groups on their surface. In solution, binding of His6-tagged green fluorescent protein (EGFP) and red fluorescent protein (RFP) to the vesicle surface was quantified by fluorescence correlation spectroscopy. Small-angle X-ray scattering indicates an increase of the membrane thickness by 2-3 nm upon protein binding. Block copolymer monolayers at the air-water interface and on solid support served as a model system to characterize the protein-decorated membranes by Brewster angle microscopy and AFM. High resolution AFM of solid-supported, hydrated monolayers indicates that the proteins form densely packed and partially ordered arrays with the cylindrically shaped EGFP molecules lying flat on the surface of the films. KW - Amphiphilic copolymer KW - Metal centers KW - His-tag proteins KW - Molecular recognition PY - 2010 DO - https://doi.org/10.1039/c002838j SN - 1744-683X VL - 6 SP - 2815 EP - 2824 PB - RSC Publ. CY - Cambridge AN - OPUS4-21564 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Mantion, Alexandre T1 - Peptide-coated silver as new building blocks for the creation of photonic crystals T2 - Photonic Materials PHONA Worshop CY - Jena, Germany DA - 2010-03-24 PY - 2010 AN - OPUS4-21036 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Mantion, Alexandre T1 - Cytotoxity of silver nanoparticles in human macrophages: Link to oxidative stress, alteration in membrane lipid composition and functional impairment T2 - E-MRS Meeting CY - Strasbourg, France DA - 2010-06-07 PY - 2010 AN - OPUS4-21403 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Mantion, Alexandre T1 - Kolloidale Eigenschaften von Peptid-beschichteten Silber-Nanopartikeln: von Modell zur Toxicologie T2 - nANO meets water II CY - Oberhausen, Germany DA - 2010-11-11 PY - 2010 AN - OPUS4-22575 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Mantion, Alexandre T1 - Silver nanoparticles and synchrotron light-based nanotoxicology: new imaging modalities T2 - JUM@P´11: Second Joint Users´ Meeting@PSI, Paul Scherrer-Institut CY - Villigen, Switzerland DA - 2011-09-15 PY - 2011 AN - OPUS4-23979 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Mantion, Alexandre T1 - New chiral peptide-coated silver nanoparticles for Material Science and Toxicology T2 - 25th ECIS Conference and the 45. Hauptversammlung der Kolloidgesellschaft CY - Berlin, Germany DA - 2011-09-04 PY - 2011 AN - OPUS4-23977 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Mantion, Alexandre T1 - New silver nanoparticles for imaging applications: Initial data on their effects in cell culture model systems T2 - ANAKON 2010 CY - Zurich, Switzerland DA - 2010-03-23 PY - 2010 AN - OPUS4-24078 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Mantion, Alexandre T1 - A novel type of silver nanoparticles and their advantages in toxicity testing in cell culture systems T2 - 5. Innovationskongress Chemie und Biotechnologie /Kleine Partikel - große Chancen CY - Potsdam, Germany DA - 2011-05-19 PY - 2011 AN - OPUS4-24060 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Mantion, Alexandre T1 - A novel type of silver nanoparticles and their advantages in toxicity testing in cell culture systems T2 - INRS Occupational Health Research Conference 2011: Risks associated to Nanoparticles and Nanomaterials CY - Nancy, France DA - 2011-04-05 PY - 2011 AN - OPUS4-24259 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Mantion, Alexandre T1 - Cytotoxicity of model silver nanoparticles in human macrophages T2 - Post-satellite Meeting Eurotox 2009 CY - Dresden, Germany DA - 2010-04-23 PY - 2010 AN - OPUS4-21038 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Mantion, Alexandre T1 - Cytotoxicity of model silver nanoparticles in human macrophages T2 - Nanotoxicology 2010 CY - Edinburgh, Scotland DA - 2010-06-02 PY - 2010 AN - OPUS4-21037 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Mantion, Alexandre T1 - A novel type of silver nanoparticles and their advantages in toxicity testing in cell systems T2 - 5th International Conference on Nanotechnology- Occupational and Environmental Health CY - Boston, MA, USA DA - 2011-08-09 PY - 2011 AN - OPUS4-24226 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Mantion, Alexandre A1 - Graf, P. A1 - Florea, I. A1 - Haase, A. A1 - Thünemann, Andreas A1 - Masic, A. A1 - Ersen, O. A1 - Rabu, P. A1 - Meier, W. A1 - Luch, A. A1 - Taubert, A. T1 - Biomimetic synthesis of chiral erbium-doped silver/peptide/silica core-shell nanoparticles (ESPN) N2 - Peptide-modified silver nanoparticles have been coated with an erbium-doped silica layer using a method inspired by silica biomineralization. Electron microscopy and small-angle X-ray scattering confirm the presence of an Ag/peptide core and silica shell. The erbium is present as small Er2O3 particles in and on the silica shell. Raman, IR, UV-Vis, and circular dichroism spectroscopies show that the peptide is still present after shell formation and the nanoparticles conserve a chiral plasmon resonance. Magnetic measurements find a paramagnetic behavior. In vitro tests using a macrophage cell line model show that the resulting multicomponent nanoparticles have a low toxicity for macrophages, even on partial dissolution of the silica shell. KW - Nanoparticle KW - Small-angle X-ray scattering KW - SAXS PY - 2011 DO - https://doi.org/10.1039/c1nr10930h SN - 2040-3364 SN - 2040-3372 VL - 3 IS - 12 SP - 5168 EP - 5179 PB - RSC Publ. CY - Cambridge AN - OPUS4-25422 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Niehoff, A. A1 - Mantion, Alexandre A1 - McAloney, R. A1 - Huber, Alexandra A1 - Falkenhagen, Jana A1 - Goh, C.M. A1 - Thünemann, Andreas A1 - Winnik, M. A. A1 - Menzel, H. T1 - Elucidation of the structure of poly(gamma-benzyl-L-glutamate) nanofibers and gel networks in a helicogenic solvent N2 - The synthesis, characterization, self-assembly, and gel formation of poly(γ-benzyl-L-glutamate) (PBLG) in a molecular weight range from ca. 7,000–100,000 g/mol and with narrow molecular weight distribution are described. The PBLG is synthesized by the nickel-mediated ring-opening polymerization and is characterized by size-exclusion chromatography coupled with multiple-angle laser light scattering, NMR, and Fourier transform infrared spectroscopy. The self-assembly and thermoreversible gel formation in the helicogenic solvent toluene is investigated by transmission electron microscopy, atomic force microscopy, small-angle X-ray scattering, and synchrotron powder X-ray diffraction. At concentrations significantly below the minimum gelation concentration, spherical aggregates are observed. At higher concentrations, gels are formed, which show a 3D network structure composed of nanofibers. The proposed self-assembly mechanism is based on a distorted hexagonal packing of PBLG helices parallel to the axis of the nanofiber. The gel network forms due to branching and rejoining of bundles of PBLG nanofibers. The network exhibits uniform domains with a length of 200±42 nm composed of densely packed PBLG helices. KW - Poly(gamma-benzyl-L-glutamate) (PBLG) KW - Nickel-mediated NCA polymerization KW - Thermoreversible gel formation KW - Physical/supramolecular organogel KW - Nanofiber KW - Self-assembly KW - Alpha-helix KW - Nanotechnology KW - Small-angle X-ray scatering KW - SAXS PY - 2013 DO - https://doi.org/10.1007/s00396-012-2866-9 SN - 0303-402X SN - 1435-1536 VL - 291 IS - 6 SP - 1353 EP - 1363 PB - Springer CY - Berlin AN - OPUS4-28615 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -