TY - JOUR A1 - Ibrahim, B. A1 - Arkhipova, K. A1 - Andeweg, A.C. A1 - Posada-Céspedes, S. A1 - Enault, F. A1 - Gruber, A. A1 - Koonin, E.V. A1 - Kupczok, A. A1 - Lemey, P. A1 - McHardy, A.C. A1 - McMahon, Dino Peter A1 - Pickett, B.E. A1 - Robertson, D.L. A1 - Scheuermann, R.H. A1 - Zhernakova, A. A1 - Zwart, M.P. A1 - Schönhuth, A. A1 - Dutilh, B.E. A1 - Marz, M. T1 - Bioinformatics meets virology: The European virus bioinformatics center's second annual meeting N2 - The Second Annual Meeting of the European Virus Bioinformatics Center (EVBC), held in Utrecht, Netherlands, focused on computational approaches in virology, with topics including (but not limited to) virus discovery, diagnostics, (meta-)genomics, modeling, epidemiology, molecular structure, evolution, and viral ecology. The goals of the Second Annual Meeting were threefold: (i) to bring together virologists and bioinformaticians from across the academic, industrial, professional, and training sectors to share best practice; (ii) to provide a meaningful and interactive scientific environment to promote discussion and collaboration between students, postdoctoral fellows, and both new and established investigators; (iii) to inspire and suggest new research directions and questions. Approximately 120 researchers from around the world attended the Second Annual Meeting of the EVBC this year, including 15 renowned international speakers. This report presents an overview of new developments and novel research findings that emerged during the meeting. KW - Bioinformatics KW - Software KW - Virology KW - Viruses PY - 2018 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-458814 DO - https://doi.org/10.3390/v10050256 SN - 1999-4915 VL - 10 IS - 5 SP - 256, 1 EP - 19 PB - MDPI AN - OPUS4-45881 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - RPRT A1 - Röhl-Kuhn, Barbara A1 - Klobes, Peter A1 - Meyer, Klaus A1 - Lorenz, Peter A1 - Lamberty, A. A1 - Gawlik, B. A1 - Muntau, H. T1 - Certification of the specific micropore volume and the median width of two microporous reference materials according to Draft-DIN 66135-4 KW - Poröse Materialien KW - ZRM-Entwicklung KW - Gasadsorption KW - Mikroporenbestimmung PY - 2004 UR - https://ec.europa.eu/jrc/sites/default/files/rm/BCR-704_report.pdf SN - 1018-5593 IS - 21065 SP - I-III, 5-38 PB - Office for Official Publications of the European Communities CY - Luxembourg AN - OPUS4-5195 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Kettschau, A. A1 - Goebbels, Jürgen A1 - Reimers, Peter A1 - Weise, Hans-Peter ED - Merz, E. T1 - Possibilities of quality assurance on radioactive waste packages by computertomography and other radiometric techniques T2 - International Seminar on Radioactive Waste Products - Suitability for Final Disposal CY - Jülich, Germany DA - 1985-06-10 PY - 1985 N1 - Serientitel: Berichte der Kernforschungsanlage Jülich – Series title: Berichte der Kernforschungsanlage Jülich IS - 54 SP - 483 EP - 491 PB - Zentralbibliothek der Kernforschungsanlage CY - Jülich AN - OPUS4-34222 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Doublet, V. A1 - Poeschl, Y. A1 - Gogol-Döring, A. A1 - Alaux, C. A1 - Annoscia, D. A1 - Aurori, C. A1 - Barribeau, S. M. A1 - Bedoya-Reina, O. C. A1 - Brown, M. J. F. A1 - Bull, J. C. A1 - Flenniken, M. L. A1 - Galbraith, D. A. A1 - Genersch, E. A1 - Gisder, S. A1 - Grosse, I. A1 - Holt, H. L. A1 - Hultmark, D. A1 - Lattorff, H. M. G. A1 - Le Conte, Y. A1 - Manfredini, F. A1 - McMahon, Dino Peter A1 - Moritz, R. F. A. A1 - Nazzi, F. A1 - Niño, E. L. A1 - Nowick, K. A1 - Van Rij, R. P. A1 - Paxton, R. J. A1 - Grozinger, C. M. T1 - Unity in defence: honeybee workers exhibit conserved molecular responses to diverse pathogens N2 - Background: Organisms typically face infection by diverse pathogens, and hosts are thought to have developed specific responses to each type of pathogen they encounter. The advent of transcriptomics now makes it possible to test this hypothesis and compare host gene expression responses to multiple pathogens at a genome-wide scale. Here, we performed a meta-analysis of multiple published and new transcriptomes using a newly developed bioinformatics approach that filters genes based on their expression profile across datasets. Thereby, we identified common and unique molecular responses of a model host species, the honey bee (Apis mellifera), to its major pathogens and parasites: the Microsporidia Nosema apis and Nosema ceranae, RNA viruses, and the ectoparasitic mite Varroa destructor, which transmits viruses. Results: We identified a common suite of genes and conserved molecular pathways that respond to all investigated pathogens, a result that suggests a commonality in response mechanisms to diverse pathogens. We found that genes differentially expressed after infection exhibit a higher evolutionary rate than non-differentially expressed genes. Using our new bioinformatics approach, we unveiled additional pathogen-specific responses of honey bees; we found that apoptosis appeared to be an important response following microsporidian infection, while genes from the immune signalling pathways, Toll and Imd, were differentially expressed after Varroa/virus infection. Finally, we applied our bioinformatics approach and generated a gene co-expression network to identify highly connected (hub) genes that may represent important mediators and regulators of anti-pathogen responses. Conclusions: Our meta-analysis generated a comprehensive overview of the host metabolic and other biological processes that mediate interactions between insects and their pathogens. We identified key host genes and pathways that respond to phylogenetically diverse pathogens, representing an important source for future functional studies as well as offering new routes to identify or generate pathogen resilient honey bee stocks. The statistical and bioinformatics approaches that were developed for this study are broadly applicable to synthesize information across transcriptomic datasets. These approaches will likely have utility in addressing a variety of biological questions. KW - Coexpression KW - Apis mellifera KW - Nosema KW - Varroa destructor KW - DWV KW - IAPV KW - RNA virus KW - Meta-analysis KW - Transcriptomics PY - 2017 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-410439 DO - https://doi.org/10.1186/s12864-017-3597-6 SN - 1471-2164 VL - 18 SP - 207 EP - 224 AN - OPUS4-41043 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Jung, C. M. A1 - Heintz, A. A1 - Kraus, Werner A1 - Wunderlich, G. A1 - Leibnitz, Peter A1 - Pietzsch, H.-J. A1 - Kropp, J. A1 - Deussen, A. A1 - Spies, H. ED - Marino, N. T1 - Technetium- and Rhenium-Labelled Fatty Acids as Model Compounds for Myocardial Metabolism Imaging N2 - In an attempt to develop new technetium-based radiopharmaceuticals for the non-invasive diagnosis of oxidative myocardial metabolism, rhenium model compounds according to the ‚3+1' mixed ligand approach as well as the organometallic tricarbonyl-design were synthesized. The geometrical impact of different chelates on the integrity of the fatty acid head structure was determined by single crystal X-ray analyses. To evaluate the diagnostic potential of the analogous Technetium-99m compounds, fatty acid complexes of the ‚3+1' mixed ligand type were prepared on n.c.a.-level and studied in the isolated constant-flow-perfused guinea pig heart model; compared to established [123I]Iodine-labelled fatty acid radiotracers, the tested Technetium-99m derivatives showed a specific, however significantly lower myocardial extraction rate. T2 - 6th International Symposium on Technetium in Chemistry and Nuclear Medicine CY - Bressanone, Italy DA - 2002-09-04 KW - Fatty acids KW - Metabolism KW - Myocardial Imaging KW - Rhenium KW - Technetium-99m PY - 2002 UR - http://www.fzd.de/db/!Publications?pSelYear=2002&pLang=en&pNid=0&pSelWithSubmitted=0&pSelSort=TITEL&pSelPublForm=4&pSelPage=2 SN - 88-86281-73-0 N1 - Serientitel: Technetium, rhenium and other metals in chemistry and nuclear medicine – Series title: Technetium, rhenium and other metals in chemistry and nuclear medicine VL - 6 IS - 6 SP - 443 EP - 445 PB - SGEditoriali CY - Padova AN - OPUS4-1999 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Bleck, W. A1 - Bork, Claus-Peter A1 - Engl, B. A1 - Evertz, Th. A1 - Frehn, A. A1 - Hatscher, A. A1 - Hinterdorfer, J. A1 - Kaufmann, H. A1 - Masendorf, R. A1 - Nicklas, D. A1 - Sonne, H.-M. A1 - Sonsino, C.M. A1 - Steinbeck, G. A1 - Zenner, H. T1 - Ermittlung von Berechnungskennwerten für Karosseriestähle KW - Feinblech KW - Kennwerte KW - FEM-Simulation KW - Automobilindustrie PY - 2004 SN - 0340-4803 SN - 0340-479x VL - 124 IS - 11 SP - 137 EP - 140 PB - Verl. Stahleisen CY - Düsseldorf AN - OPUS4-5834 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Steinbeck, G. A1 - Bleck, W. A1 - Bork, Claus-Peter A1 - Engl, B. A1 - Evertz, Th. A1 - Frehn, A. A1 - Hatscher, A. A1 - Hinterdorfer, J. A1 - Kaufmann, H. A1 - Masendorf, R. A1 - Nicklas, D. A1 - Sonne, H.-M. A1 - Sonsino, C. M. A1 - Zenner, H. ED - Zenner, H. T1 - Eigenschaften und Berechnungskennwerte von Karosseriestählen T2 - DVM-Tag 2005 CY - Berlin, Deutschland DA - 2005-04-27 KW - Feinblech KW - Kennwerte KW - FE-Simulation KW - Automobilindustrie PY - 2005 SN - 0946-5987 N1 - Serientitel: DVM Bericht – Series title: DVM Bericht IS - 672 SP - 133 EP - 146 PB - DVM CY - Düsseldorf AN - OPUS4-10652 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Sieksmeyer, T. A1 - He, S. A1 - Esparza-Mora, M. A. A1 - Jiang, S. A1 - Petrasiunaite, V. A1 - Kuropka, B. A1 - Banasiak, Robert A1 - Julseth, M. J. A1 - Weise, C. A1 - Johnston, P. R. A1 - Rodriguez-Rojas, A. A1 - McMahon, Dino Peter T1 - Eating in a losing cause: Limited benefit of modifed macronutrient consumption following infection in the oriental cockroach Blatta orientalis N2 - Background: Host–pathogen interactions can lead to dramatic changes in host feeding behaviour. One aspect of this includes self-medication, where infected individuals consume substances such as toxins or alter their macronutrient consumption to enhance immune competence. Another widely adopted animal response to infection is illness-induced anorexia, which is thought to assist host immunity directly or by limiting the nutritional resources available to pathogens. Here, we recorded macronutrient preferences of the global pest cockroach, Blatta orientalis to investigate how shifts in host macronutrient dietary preference and quantity of carbohydrate (C) and protein (P) interact with immunity following bacterial infection. Results: We fnd that B. orientalis avoids diets enriched for P under normal conditions, and that high P diets reduce cockroach survival in the long term. However, following bacterial challenge, cockroaches signifcantly reduced their overall nutrient intake, particularly of carbohydrates, and increased the relative ratio of protein (P:C) consumed. Surprisingly, these behavioural shifts had a limited efect on cockroach immunity and survival, with minor changes to immune protein abundance and antimicrobial activity between individuals placed on diferent diets, regardless of infection status. Conclusions: We show that cockroach feeding behaviour can be modulated by a pathogen, resulting in an illness-induced anorexia-like feeding response and a shift from a C-enriched to a more P:C equal diet. However, our results also indicate that such responses do not provide signifcant immune protection in B. orientalis, suggesting that the host’s dietary shift might also result from random rather than directed behaviour. The lack of an apparent beneft of the shift in feeding behaviour highlights a possible reduced importance of diet in immune regulation in these invasive animals, although further investigations employing pathogens with alternative infection strategies are warranted. KW - Animal immune system KW - A key interface KW - Host and symbiont ecology KW - Behavioural mechanisms KW - Biotic environment PY - 2022 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-550022 DO - https://doi.org/10.1186/s12862-022-02007-8 SN - 2730-7182 VL - 22 IS - 1 SP - 1 EP - 14 PB - Springer Nature CY - London, UK AN - OPUS4-55002 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Tauber, J. P. A1 - McMahon, Dino Peter A1 - Ryabov, E.V. A1 - Kunat, M. A1 - Ptaszynska, A. A. A1 - Evans, J. D. T1 - Honeybee intestines retain low yeast titers, but no bacterial mutualists, at emergence N2 - Honeybee symbionts, predominantly bacteria, play important roles in honeybee health, nutrition, and pathogen protection, thereby supporting colony health. On the other hand, fungi are often considered indicators of poor bee health, and honeybee microbiome studies generally exclude fungi and yeasts. We hypothesized that yeasts may be an important aspect of early honeybee biology, and if yeasts provide a mutual benefit to their hosts, then honeybees could provide a refuge during metamorphosis to ensure the presence of yeasts at emergence. We surveyed for yeast and fungi during pupal development and metamorphosis in worker bees using fungal-specific quantitative polymerase chain reaction (qPCR), next-generation sequencing, and standard microbiological culturing. On the basis of yeast presence in three distinct apiaries and multiple developmental stages, we conclude that yeasts can survive through metamorphosis and in naïve worker bees, albeit at relatively low levels. In comparison, known bacterial mutualists, like Gilliamella and Snodgrassella, were generally not found in pre-eclosed adult bees. Whether yeasts are actively retained as an important part of the bee microbiota or are passively propagating in the colony remains unknown. Our demonstration of the constancy of yeasts throughout development provides a framework to further understand the honeybee microbiota. KW - Fungi KW - Honeybee KW - Microbiota KW - Yeast PY - 2022 DO - https://doi.org/10.1002/yea.3665 SN - 1097-0061 VL - 39 IS - 1-2 SP - 95 EP - 107 PB - John Wiley & Sons Ltd. CY - London, UK AN - OPUS4-53892 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Driscoll, Laura L. A1 - Driscoll, Elizabeth H. A1 - Dong, Bo A1 - Sayed, Farheen N. A1 - Wilson, Jacob N. A1 - O’Keefe, Christopher A. A1 - Gardner, Dominic J. A1 - Grey, Clare P. A1 - Allan, Phoebe K. A1 - Michalchuk, Adam A. L. A1 - Slater, Peter R. T1 - Under pressure: offering fundamental insight into structural changes on ball milling battery materials N2 - Synthesis of Li ion battery materials via ball milling has been a huge area of growth, leading to new high-capacity electrode materials, such as a number of promising disordered rocksalt (DRS) phases. In prior work, it was generally assumed that the synthesis was facilitated simply by local heating effects during the milling process. In this work, we show that ball milling Li2MoO4 leads to a phase transformation to the high pressure spinel polymorph and we report electrochemical data for this phase. This observation of the formation of a high pressure polymorph shows that local heating effects alone cannot explain the phase transformation observed (phenakite to spinel) and so indicates the importance of other effects. In particular, we propose that when the milling balls collide with the material, the resulting shockwaves exert a localised pressure effect, in addition to local heating. To provide further support for this, we additionally report ball milling results for a number of case studies (Li2MnO3, Li2SnO3, Nb2O5) which reinforces the conclusion that local heating alone cannot explain the phase transformations observed. The work presented thus provides greater fundamental understanding of milling as a synthetic pathway and suggests potential strategies to prepare such samples without milling (e.g., doping to create internal chemical pressure). In addition, we suggest that further research is needed into the effect of the use of milling as a route to smaller particles, since we believe that such milling may also be affecting the surface structure of the particles through the influence of the shockwaves generated. KW - Pollution KW - Nuclear Energy and Engineering KW - Sustainability and the Environment KW - Environmental Chemistry KW - Renewable Energy PY - 2023 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-590086 DO - https://doi.org/10.1039/d3ee00249g VL - 16 IS - 11 SP - 5196 EP - 5209 PB - Royal Society of Chemistry (RSC) AN - OPUS4-59008 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Shaik, A.-A. A1 - Richter, M. A1 - Kricheldorf, H. A1 - Krüger, Ralph-Peter T1 - New polymer syntheses - CIX. Biodegradable, alternating copolyesters of terephthalic acid, aliphatic dicarboxylic acids, and alkane diols N2 - Copolyesters with an alternating sequence of terephthalic acid and aliphatic dicarboxylic acids were prepared with three different methods. First, dicarboxylic acid dichlorides were reacted with bis(2-hydroxyethyl)terephthalate (BHET) in refluxing 1,2-dichlorobenzene. Second, the same monomers were polycondensed at 0-20 °C in the presence of pyridine. Third, dicarboxylic acid dichlorides and silylated BHET were polycondensed in bulk. Only this third method gave satisfactory molecular weights. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry revealed that the copolyesters prepared by the pyridine and silyl methods might have contained considerable fractions of cyclic oligoesters and polyesters despite the absence of transesterification and backbiting processes. The alternating sequences and thermal properties were characterized with 1H NMR spectroscopy and differential scanning calorimetry measurements, respectively. In agreement with the alternating sequence, all copolyesters proved to be crystalline, but the crystallization was extremely slow [slower than that of poly(ethylene terephthalate)]. A second series of alternating copolyesters was prepared by the polycondensation of silylated bis(4-hydroxybut- yl)terephthalate with various aliphatic dicarboxylic acid dichlorides. The resulting copolyesters showed significantly higher rates of crystallization, and the melting temperatures were higher than those of the BHET-based copolyesters. © 2001 John Wiley & Sons, Inc. J Polym Sci Part A: Polym Chem 39: 3371-3382, 2001 KW - Poly(ethylene terephthalate) KW - Poly(butylene terephthalate) KW - Alternating sequence KW - Macrocycles KW - Crystallization KW - Biodegradable polyesters PY - 2001 DO - https://doi.org/10.1002/pola.1320 SN - 0360-6376 SN - 0887-624X VL - 39 IS - 19 SP - 3371 EP - 3382 PB - Wiley CY - Hoboken, NJ AN - OPUS4-2173 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schlaad, H. A1 - Müller, A. A1 - Spickermann, J. A1 - Räder, H. A1 - Krüger, Ralph-Peter T1 - Quantitative Analysis of Molecular Weight Distribution Obtained by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) T2 - 2nd International Conference on Molecular Mass Characterization of Synthetic and Natural Polymers CY - Bradford, England, UK DA - 1996-04-01 PY - 1996 CY - Bradford AN - OPUS4-2218 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Spickermann, J. A1 - Martin, K. A1 - Räder, H.J. A1 - Müllen, K. A1 - Schlaad, H. A1 - Müller, A. H. E. A1 - Krüger, Ralph-Peter T1 - Quantitative analysis of broad molecular weight distributions obtained by MALDI-TOF mass spectrometry PY - 1996 SN - 1469-0667 SN - 1356-1049 SN - 1365-0718 IS - 2 SP - 161 EP - 165 PB - IM Publications CY - Chichester AN - OPUS4-2153 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Müller, A. A1 - Krüger, Ralph-Peter T1 - Quantitative analysis of molecular weight distribution obtained by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry T2 - DECHEMA-Diskussionstagung "Neue Methoden der Polymeranalytik" CY - Mainz, Germany DA - 1996-10-07 PY - 1996 CY - Mainz AN - OPUS4-2224 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Klobes, Peter A1 - Minor, D. B. A1 - Röhl-Kuhn, B. A1 - Zimathies, Annett A1 - Prinz, C. A1 - Leigh, S. D. A1 - Heckert, N. A. A1 - MacDonald, B. S. T1 - Porosimetrie-Standard NIST SRM 1917 / CRM BAM-P 127 - Das erste von NIST und BAM gemeinsam zertifizierte Referenzmaterial T2 - 6. Workshop über die Charakterisierung von Feinteiligen und Porösen Festkörpern CY - Bad Soden, Deutschland DA - 2002-11-14 PY - 2002 CY - Bad Soden AN - OPUS4-1500 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Kley, Gerd A1 - Deike, R. A1 - Freudenberg, A. A1 - Köcher, Peter A1 - Schommer, R. T1 - Verwertung von Klärschlammasche in der Metallurgie N2 - Es wird ein Großversuch beschrieben, in dem der Nachweis erbracht wird, dass Klärschlammasche, die einen hohen Anteil an P und Fe aufweist, in der Metallurgie als Rohstoffkomponente für Roheisen mit einem erhöhten Phosphorgehalt eingesetzt werden kann. Der zunächst mit ca. 100 t Klärschlammasche hergestellte Sinter ließ sich ähnlich dem konventionell hergestellten Sinter im Hochofen zu Roheisen verarbeiten. Roheisen mit erhöhtem Phosphoranteil wird für die Herstellung von filigranen und dünnwandigen Gußstücken verwendet. PY - 2001 SN - 0027-2957 SN - 1863-9763 VL - 33 IS - 12 SP - 672 EP - 676 PB - Erich Schmidt CY - Berlin AN - OPUS4-1226 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Pietzsch, H.-J. A1 - Seifert, S. A1 - Drews, A. A1 - Syhre, R. A1 - Spies, H. A1 - Leibnitz, Peter A1 - Tisato, F. A1 - Refosco, F. T1 - Novel technetium(III) mixed-ligand chelates for the design of lipophilic complexes stable in vivo PY - 2001 UR - http://www3.interscience.wiley.com/journal/3209/toc SN - 0362-4803 SN - 1099-1344 SN - 0022-2135 VL - 44 IS - 1 SP - 39 PB - Wiley CY - London AN - OPUS4-1557 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schlaad, H. A1 - Müller, A. H. E. A1 - Kolshorn, H. A1 - Krüger, Ralph-Peter T1 - Mechanism of anionic polymerization of (meth)acrylates in the presence of aluminium alkyls PY - 1995 SN - 0170-0839 SN - 1436-2449 VL - 35 SP - 169 EP - 176 PB - Springer CY - Berlin AN - OPUS4-2144 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Sonnenschein, H. A1 - Kreher, T. A1 - Gründemann, E. A1 - Krüger, Ralph-Peter A1 - Kunath, A. A1 - Zabel, V. T1 - Novel Redox-Active Cyclophanes Based on 3,3'-Bisindolizines - Synthesis and Chirality PY - 1996 SN - 0022-3263 SN - 1520-6904 VL - 61 SP - 710 EP - 714 PB - American Chemical Society CY - Washington, DC AN - OPUS4-2147 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Holtappels, Kai A1 - Dietlen, Siegmund A1 - Schulze, Heinz-Peter A1 - Stickling, J. A1 - Schönbucher, A. T1 - Experimentelle Bestimmung des Inert- und Brenngaseinflusses auf die Stabilitätsgrenzdrücke von Ethin PY - 2003 DO - https://doi.org/10.1002/cite.200303192 SN - 0009-286X SN - 1522-2640 VL - 75 IS - 7 SP - 909 EP - 912 PB - Wiley-VCH Verl. CY - Weinheim AN - OPUS4-2611 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Hesselbach, J. A1 - Herrmann, C. A1 - Bock, R. A1 - Dettmer, T. A1 - Kley, Gerd A1 - Köcher, Peter A1 - Brenneis, Rudolf A1 - Meyer-Pittroff, R. A1 - Falk, O. A1 - Hansen, A. T1 - Kühlschmierstoffe aus technischen tierischen Fetten und Altspeisefetten - Herstellung, Technologie und Ökobilanzierung KW - Enzymatische Alkoholyse KW - Tierfett KW - Altspeisefett KW - Kühlschmierstoff KW - Umesterung KW - Produktökobilanz KW - LCA PY - 2003 SN - 1435-5272 VL - 9 IS - Sonderheft `Biokatalyse´ SP - 24 EP - 27 PB - BIOCOM CY - Berlin AN - OPUS4-2684 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - RPRT A1 - Mielke, Werner A1 - Hampe, Andreas A1 - Lützow, Wolfgang A1 - Müller, Hansjörg A1 - Pastuska, Gerhard A1 - Rook, A. A1 - Schnabel, E. A1 - Sickfeld, Jürgen A1 - Tobisch, Karl A1 - Trubiroha, Peter T1 - Einsatz von Kunststoffen in Pkw - Literaturstudie PY - 1990 SN - 0937-1214 IS - 2 SP - 9 EP - 257 PB - Bundesanstalt für Straßenwesen CY - Bergisch Gladbach AN - OPUS4-11796 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pfennig, A. A1 - Wolf, Marcus A1 - Wiegand, Reiner A1 - Bork, Claus-Peter T1 - New in-situ measurement technique to determine corrosion fatigue in components and pipes under cyclic load N2 - In the field of water pipelines, geothermal energy production as well as carbon capture and storage technology (CCS) materials have to provide a high resistance to corrosion and mechanical stress. The combination of cyclic load and corrosive aqueous environment leads to corrosion fatigue of pipes and components (e.g. pumps) and thus inevitably to the reduction of the lifetime of these components. To estimate the reliability of components from adjusted in-situ-laboratory experiments a corrosion chamber was designed and tested with CO2 saturated corrosive aqueous media flowing at a steady rate. Unique feature of this special chamber is its installation directly onto the sample and thus providing flexible usability in almost every testing machine. This allows simultaneous mechanical loading of the sample, operation at temperatures up to 100 °C and exposure to fluid flow of corrosive liquids and gases. The lifetime reduction of AISI 420C (X46Cr13, 1.4034) is demonstrated at T=60 °C, geothermal brine: Stuttgart Aquifer flow rate: 9 l/h, CO2. S-N plots, micrographic-, phase-, fractographic- and surface analysis were applied to obtain sustainable information on the corrosion fatigue behavior. Maximum number of cycles (here 12.5 x 106 cycles to failure) is reached at σa =173 MPa. No typical fatigue strength exists and passive corrosion fatigue may be identified as failure cause. T2 - MWWD & IEMES 2012 CY - Budva, Montenegro DA - 22.10.2012 KW - Corrosion fatigue KW - Corrosion chamber KW - S-N-plots KW - Steel KW - In-situ experiment PY - 2012 SN - 978-9944-5566-6-8 SP - 1 EP - 8 AN - OPUS4-28569 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Pfennig, A. A1 - Wiegand, Reiner A1 - Bork, Claus-Peter T1 - Corrosion and corrosion fatigue of AISI 420C (X46Cr13) at 60 °C in CO2-saturated artificial geothermal brine N2 - The lifetime reduction of cyclically loaded AISI 420C (X46Cr13, 1.4034) constantly exposed to highly corrosive CO2-saturated hot thermal water is demonstrated in in situ-laboratory experiments (60 °C, brine: Stuttgart Aquifer, flowing CO2: 30 L/h). S–N plots, micrographic-, phase-, fractographic- and surface analysis were applied to obtain sustainable information on the corrosion and corrosion fatigue behavior. Maximum number of cycles (here 12.5 × 106 cycles to failure) is reached at σa = 173 MPa. Hydroxide and siderite layers were found on pits and crack surfaces. No typical fatigue limit exists and pit corrosion prior to crack initiation may be identified as failure cause. KW - A. Stainless steel KW - C. Corrosion fatigue KW - C. Pitting corrosion KW - C. Kinetic parameters KW - B. SEM PY - 2013 DO - https://doi.org/10.1016/j.corsci.2012.11.005 SN - 0010-938X VL - 68 SP - 134 EP - 143 PB - Elsevier CY - Orlando, Fla. AN - OPUS4-27541 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Klobes, Peter A1 - Thormann, A. A1 - Emmerling, Franziska A1 - Zimathies, Annett A1 - Heilmann, A. T1 - Porosity Characterisation of Nanoporous Alumina Membranes T2 - XII. Workshop über die Charakterisierung von feinteiligen und porösen Festkörpern CY - Bad Soden, Germany DA - 2008-11-18 PY - 2008 AN - OPUS4-18148 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Bäumer, A. A1 - Bork, Claus-Peter A1 - Emde, T. A1 - Evertz, T. A1 - Masendorf, R. A1 - May, U. A1 - Menne, M. A1 - Ratte, E. A1 - Steinbeck, G. T1 - Forschungsvorhaben P 603 - Ermittlung des Werkstoffverhaltens und des Beschichtungseinflusses durch rechnerische Methoden zur Verkürzung der Entwicklungszeiten im Fahrzeugbau mit Stahl KW - Stahlblech KW - Automobilindustrie KW - Crashverhalten KW - Fließkurven KW - Dehnungswöhlerlinien KW - Lebensdauerabschätzung PY - 2007 SN - 3-937567-48-8 SP - 122 Seiten PB - Verlag und Vertriebsgesellschaft mbH CY - Düsseldorf AN - OPUS4-15748 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Bleck, W. A1 - Bork, Claus-Peter A1 - Evertz, Th. A1 - Frehn, A. A1 - Masendorf, R. A1 - Sonsino, C.M. A1 - Steinbeck, G. T1 - Ermittlung von Berechnungskennwerten an Karosseriestählen N2 - In einem Gemeinschaftsprojekt der Stahl- und Automobilindustrie wurden an zwanzig Stählen für Feinblech elastische, plastische und zyklische Kennwerte für die FE-Simulation ermittelt. Die Untersuchungen mit dem Ziel, Eingabekennwerte für FE-Berechnungen zu erhalten, erbrachten einen erheblichen Erfahrungszuwachs im Bereich der Messtechnik sowie der Datenerfassung und -weiterverarbeitung. Der vorliegende Beitrag gibt einen Überblick über die Untersuchung und ihre Ergebnisse. KW - Feinblech Stähle KW - Kennwerte für die FEM-Simulation KW - Elastisch KW - Plastisch KW - Zyklisch KW - Standardisierte Kennwertermittlung KW - Rechnerisch PY - 2005 SN - 0001-2785 VL - 107 IS - 5 SP - 436 EP - 447 PB - Vieweg CY - Wiesbaden AN - OPUS4-10654 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Krüger, Ralph-Peter A1 - Heinemann, M. A1 - Falkenhagen, Jana A1 - Hartmann, A. A1 - Friedrich, Jörg Florian A1 - Fritz, H.-G. ED - Fritz, H. ED - Eisenbach, C. T1 - Charakterisierung biologisch abbaubarer Homo- und Copolyester / Characterisation of biodegradable Homo- and Copolyesters T2 - 19. Stuttgarter Kunststoff-Kolloquium 2005 CY - Stuttgart, Deutschland DA - 2005-03-09 KW - MALDI-TOF-MS KW - Bioabbaubare Polyester KW - Flüssigchromatographie KW - Methodenkopplung KW - Biodegradable polyesters KW - Liquid chromatography KW - Coupling of analytical methods PY - 2005 SN - 3-00-015246-6 SP - 5/P1-1 - 5/P1-9 PB - IKT CY - Stuttgart AN - OPUS4-11767 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schulz-Forberg, Bernd A1 - Zeisler, Peter A1 - Droste, Bernhard A1 - Kondratiev, A. A1 - Kozlov, J. A1 - Tichinov, N. T1 - Soviet-German cooperation in safety improvements of spent fuel transport casks N2 - The paper gives a survey of the Soviet (Russian) -German activities which started in 1988 with the objective of creating a long-term scientific-technical cooperation in the field of transport and storage casks for spent nuclear fuel. The first step, i.e., the step of informing each other about the state of development is done. The more complicated second phase with concerted common activities of both the Russian and German competent authorities and industrial enterprises is intended to start in the near future. T2 - PATRAM ´92 - 10th International symposium on the packaging and transportation of radioactive materials CY - Yokohama, Japan DA - 1992-09-13 PY - 1992 VL - 1 SP - 343 EP - 350 AN - OPUS4-21824 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Beke, S. A1 - Sugioka, K. A1 - Midorikawa, K. A1 - Péter, Á. A1 - Nánai, L. A1 - Bonse, Jörn T1 - Characterization of the ablation of TeO2 crystals in air with femtosecond laser pulses N2 - Femtosecond (fs) laser pulse ablation (pulse duration of 150 fs, wavelength of 775 nm, repetition rate of 1 kHz) of single-crystalline TeO2 surfaces was performed in air using the direct focusing technique. The lateral and vertical dimensions of laser ablated craters as well as the laser damage thresholds were evaluated for different pulse numbers applied to the same spot. The joint observation using optical microscopy, atomic force microscopy and scanning electron microscopy revealed the surface morphology of the ablated craters and also showed that the ablation threshold depends significantly on the number of laser pulses applied to the same spot due to incubation effects. The incubation effects change the absorption processes involved in fs-laser ablation of the transparent material from multiphoton absorption to a single-photon absorption. These results are discussed on the basis of recent models of the interaction of fs-laser pulses with dielectrics. KW - Femtosecond laser ablation KW - Damage threshold KW - TeO2 KW - Dielectrics PY - 2010 DO - https://doi.org/10.1088/0022-3727/43/2/025401 SN - 0022-3727 SN - 1361-6463 VL - 43 IS - 2 SP - 025401-1 - 025401-6 PB - IOP Publ. CY - Bristol AN - OPUS4-20665 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Golian, M. A1 - Bien, T. A1 - Schmelzle, S. A1 - Esparza- Mora, M. A. A1 - McMahon, Dino Peter A1 - Dreisewerd, K. A1 - Buellesbach, J. ED - Appel, Arthur G. T1 - Neglected Very Long-Chain Hydrocarbons and the Incorporation of Body Surface Area Metrics Reveal Novel Perspectives for Cuticular Profile Analysis in Insects N2 - Most of our knowledge on insect cuticular hydrocarbons (CHCs) stems from analytical techniques based on gas-chromatography coupled with mass spectrometry (GC-MS). However, this method has its limits under standard conditions, particularly in detecting compounds beyond a chain length of around C40. Here, we compare the CHC chain length range detectable by GC-MS with the range assessed by silver-assisted laser desorption/ionization mass spectrometry (Ag-LDI-MS), a novel and rarely applied technique on insect CHCs, in seven species of the order Blattodea. For all tested species, we unveiled a considerable range of very long-chain CHCs up to C58, which are not detectable by standard GC-MS technology. This indicates that general studies on insect CHCs May frequently miss compounds in this range, and we encourage future studies to implement analytical techniques extending the conventionally accessed chain length range. Furthermore, we incorporate 3D scanned insect body surface areas as an additional factor for the comparative quantification of extracted CHC amounts between our study species. CHC quantity distributions differed considerably when adjusted for body surface areas as opposed to directly assessing extracted CHC amounts, suggesting that a more accurate evaluation of relative CHC quantities can be achieved by taking body surface areas into account. KW - Cuticular hydrocarbons KW - Blattodea KW - GC-MS KW - Ag-LDI-MS KW - Chemical ecology PY - 2022 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-543530 DO - https://doi.org/10.3390/insects13010083 VL - 13 IS - 1 SP - 2 EP - 10 PB - MDPI CY - Basel, Schweiz AN - OPUS4-54353 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - He, S. A1 - Sieksmeyer, T. A1 - Che, Y. A1 - Mora, M. A. E. A1 - Stiblik, P. A1 - Banasiak, Robert A1 - Harrison, M. C. A1 - Sobotnik, J. A1 - Wang, Z. A1 - Johnston, P. R. A1 - McMahon, Dino Peter ED - He, s. ED - McMahon, Dino Peter T1 - Evidence for reduced immune gene diversity and activity during the evolution of termites N2 - The evolution of biological complexity is associated with the emergence of bespoke immune systems that maintain and protect organism integrity. Unlike the well-studied immune systems of cells and individuals, little is known about the origins of immunity during the transition to eusociality, a major evolutionary transition comparable to the evolution of multicellular organisms from single-celled ancestors. We aimed to tackle this by characterizing the immune gene repertoire of 18 cockroach and termite species, spanning the spectrum of solitary, subsocial and eusocial lifestyles. We find that key transitions in termite sociality are correlated with immune gene family contractions. In cross-species comparisons of immune gene expression, we find evidence for a caste-specific social defence system in termites, which appears to operate at the expense of individual immune protection. Our study indicates that a major transition in organismal complexity may have entailed a fundamental reshaping of the immune system optimized for group over individual defence. KW - Social insect KW - Subsocial KW - Cockroach KW - Major transition KW - Contraction KW - Expansion PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-538878 DO - https://doi.org/10.1098/rspb.2020.3168 SN - 0962-8452 VL - 288 IS - 1945 SP - 1 EP - 10 PB - The Royal Society Publishing CY - London, UK AN - OPUS4-53887 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Fürst, Richard A1 - Häßler, Dustin A1 - Peter, A. A1 - Rohleder, R. A1 - Hothan, Sascha T1 - European test standard intumescent coatings applied to steel tension bars with solid section N2 - Intumescent coatings are used in civil engineering to improve the fire resistance of steel constructions. Due to the thin coating thickness and the profile-following application, the architectural appearance of the steel structure can be preserved. EN 13381-8 provides regulations to determine the contribution to the fire resistance of intumescent coatings applied to steel beams and columns. The scope of this standard excludes tension members and steel members with solid sections. For products that have already been successfully tested and assessed on beams and columns, EN 13381-10 offers the possibility based on unloaded fire tests to extend the scope of application of intumescent coatings to tension bars with solid sections. This approach contradicts the national safety level in Germany, where mechanically loaded testing is mandatory. Therefore, a new part of the test standard series EN 13381 is currently developed to enable the application of intumescent coatings on steel tension bars with solid section based on mechanically loaded fire tests. BAM has already carried out numerous fire tests on loaded steel tension members with intumescent coatings. Currently, in the BAM research project FIRESTEMIC, the influence of the steel bar orientation and the profile type on the thermal performance of intumescent coatings are investigated. Both questions concerning the thermal protection ability of intumescent coatings were analysed based on three different test sets, which were carried out in the tension furnace (Fig. 1a). The fire exposure corresponds to the standard temperature-time curve according to EN 1363-1. This paper summarises the main findings from the fire tests conducted in the FIRESTEMIC project. The results serve as an experimental background for the proposal of the new standard. Regarding the bar orientation, a new test setup was developed and proposed for the new standard. The two tested commercial water-based intumescent coatings with applied dry film thickness from 1.5 mm to 2.5 mm showed only a slight dependence on the bar orientation. In terms of the steel profile type, circular and rectangular solid sections with identical section factor and applied dry film thickness were tested. It was observed that the circular solid bars with diameter 30 mm heat up faster compared to the solid rectangular bars with dimension 30×30 mm (Fig. 1b). Also at larger steel bars, i.e. diameter 40 mm and dimension 40×40 mm, the same trend occurred. Thus, it is recommended for the new test standard to allow a transfer of the test results from circular to rectangular solid sections. In addition, the paper will describe and explain the test and assessment procedure proposed for the newly developed standard. T2 - 8th Symposium Structural Fire Engineering CY - Brunswick, Germany DA - 13.09.2022 KW - Fire test KW - Steel KW - Tension member KW - Intumescent coating KW - Test standard PY - 2022 SP - 1 EP - 14 PB - TU Braunschweig CY - Braunschweig AN - OPUS4-55703 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - McMahon, Dino Peter A1 - Natsopoulou, M. E. A1 - Doublet, V. A1 - Fürst, M. A1 - Weging, S. A1 - Brown, M. J. F. A1 - Gogol-Döring, A. A1 - Paxton, R. J. T1 - Elevated virulence of an emerging viral genotype as a driver of honeybee loss N2 - Emerging infectious diseases (EIDs) have contributed significantly to the current biodiversity crisis, leading to widespread epidemics and population loss. Owing to genetic variation in pathogen virulence, a complete understanding of species decline requires the accurate identification and characterization of EIDs. We explore this issue in the Western honeybee, where increasing mortality of populations in the Northern Hemisphere has caused major concern. Specifically, we investigate the importance of genetic identity of the main suspect in mortality, deformed wing virus (DWV), in driving honeybee loss. Using laboratory experiments and a systematic field survey, we demonstrate that an emerging DWV genotype (DWV-B) is more virulent than the established DWV genotype (DWV-A) and is widespread in the landscape. Furthermore, we show in a simple model that colonies infected with DWV-B collapse sooner than colonies infected with DWV-A. We also identify potential for rapid DWV evolution by revealing extensive genome-wide recombination in vivo. The emergence of DWV-B in naive honeybee populations, including via recombination with DWV-A, could be of significant ecological and economic importance. Our findings emphasize that knowledge of pathogen genetic identity and diversity is critical to understanding drivers of species decline. KW - Virulence KW - Emerging infectious disease KW - Pollinator KW - Decline PY - 2016 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-368030 DO - https://doi.org/10.1098/rspb.2016.0811 SN - 0962-8452 VL - 283 IS - 1833 SP - Article 0811, 1 EP - 8 AN - OPUS4-36803 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Bork, Claus-Peter A1 - Wolf, Marcus A1 - Pfennig, A. A1 - Trenner, S. A1 - Wiegand, Reiner T1 - Comparison between X5CrNiCuNb16-4 and X46Cr13 under corrosion fatigue N2 - In geothermal power plants materials e.g. pumps are exposed to extremely corrosive thermal water. This results in corrosion fatigue and so inevitably the reduction of the lifetime of these components. Also in the field of the carbon dioxide storage technology (carbon capture and storage CCS) components are exposed to a corrosive environment and mechanical stress. In order to gain knowledge upon the corrosion fatigue strength of materials a corrosion chamber for "in situ" conditions was designed and successfully applied.Two different steels X46Cr13 and X5CrNiCuNb16-4 have been tested and their corrosion fatigue behavior was compared. To simulate the frequency of operating pumps (30 – 40 Hz) a resonant testing machine was used. In addition technical CO2 was introduced into the closed system at a rate close to 9 L/h to keep stable environmental conditions. The samples have a surface roughness of Rz = 4 to simulate technical machined surfaces. The calculated tensile strength of X46Cr13 with soft annealed microstructure (coagulated cementite in ferrite-perlite matrix) is about 680 MPa and the yield strength is about 345 MPa. The tensile strength of X5CrNiCuNb16-4 is about 1078 MPa and the yield strength about 928 MPa. Testing parameters are: corrosion media: saline aquifer water (Stuttgart Aquifer) temperature of the brine at 60 °C and load ratio of R=-1. For X46Cr13 a stress amplitude between 160 MPa to 270 MPa and for X5CrNiCuNb16-4 a stress amplitude between 150 to 500 was chosen. Cycles until crack initiation differ strongly and were found to start at 5 x 104 up to 12.5 x 106 cycles.X46Cr13 has reached a maximum number of cycles (12.5 x 106) at a stress amplitude of 173 MPa. X5CrNiCuNb16-4 has reached the maximum number of cycles (10 x 106) at a stress amplitude of 150 MPa. The range of scatter for X5CrNiCuNb16-4 is very high (1:34) in comparison the range of scatter for X46Cr13 (1:3.5). T2 - Corrosion 2014 CY - San Antonio, TX, USA DA - 09.03.2014 KW - Steel KW - Corrosion chamber KW - Geothermal power KW - CCS KW - Corrosion fatigue PY - 2014 SP - Paper 3776, 1 EP - 6 PB - NACE AN - OPUS4-31085 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pfennig, A. A1 - Bork, Claus-Peter A1 - Wolf, Marcus T1 - Design of a high pressure system for in-situ tests on the corrosion fatigue of metallic materials N2 - In geothermal power plants pumps are exposed to corrosive and oscillating loads. This leads to corrosion fatigue and reduces the lifetime of certain components. Usually underwater power pumps are placed inside the borehole. In this case an installation depth of 600 to 800 meters below ground level is typical. In these depths an additional load due to high pressure and high temperature is introduced which is based on geothermal brine. Therefore the material which is used for the pump is steel.For fatigue testing at loads existing in deep geological layers an appropriate system for fatigue tests under in-situ conditions was set up. The requirements of the design are: temperature of the corrosion medium of 200 °C pressure of 200 bars and the complete immersion of the specimen in flowing corrosion medium containing mixtures of corrosive gasses during the entire test period.The test system has three main components the corrosion chamber the heating module and the reservoir. It is important that the corrosion chamber is only fixed onto the specimen which allows for highest flexibility of the test system operational in many different test machines. The heating module heats the corrosion medium up to 200 °C. The reservoir make sure that the volume of the corrosion medium satisfies requirements of the DIN 50905 Part 1 is conformed providing minimum required corrosive medium of 10 ml/cm² in relation to the sample surface.This innovative fatigue test system aims at performing in- situ fatigue corrosion tests simultaneously at high pressure and high temperature in liquid and gaseous corrosive environment. Results from the tests will allow determining the fatigue life of several materials influenced by pressure and temperature under corrosive conditions. T2 - Corrosion 2014 CY - San Antonio, TX, USA DA - 09.03.2014 KW - High pressure KW - Corrosion fatigue KW - Corrosion chamber PY - 2014 SP - Paper 3775, 1 EP - 6 AN - OPUS4-31086 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Robens, E. A1 - Klobes, Peter A1 - Balköse, Gulsum Devrim A1 - Amarasiri, S. A1 - Jayaweera, A. T1 - On the terms mass and weight N2 - A short survey is given on mass units and recommendations on the proper use of the notations mass and weight.Whereas mass is an inertial physical quantity in classical mechanics, weight is a force due to the gravitational field and depending on the geographic situation. KW - Atomic mass KW - Electron volt KW - Mass KW - Relative KW - Specific KW - Weight PY - 2008 DO - https://doi.org/10.1007/s10973-008-9353-z SN - 1388-6150 SN - 1418-2874 SN - 0368-4466 SN - 1572-8943 VL - 94 IS - 3 SP - 619 EP - 622 PB - Kluwer Academic Publ. CY - Dordrecht AN - OPUS4-18834 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Sonsino, C.M. A1 - Kaufmann, H. A1 - Masendorf, R. A1 - Hatscher, A. A1 - Zenner, H. A1 - Bork, Claus-Peter A1 - Hinterdorfer, J. A1 - Sonne, H.M. A1 - Engl, B. A1 - Steinbeck, G. T1 - Werkstoffkennwerte für die Lebensdauerberechnung von Strukturen aus Stahlfeinblechen für den Automobilbau N2 - Im Rahmen eines gemeinschaftlichen Projektes der Stahl- und Automobilindustrie wurden für 17 Stahlfeinbleche des Automobilbaus in verschiedenen Anlieferungs- und Verformungszuständen unter -40°C, Raumtemperatur und +100°C mit 37 Versuchsreihen Anrisswöhlerlinien und zügige bzw. zyklische Spannung-Dehnung-Kurven bestimmt. Sämtliche Versuchspunkte, Hysteresen und ermittelte zyklische Kennwerte liegen in einer Datenbank vor. Eine Korrelationen zwischen den Kennwerten aus dem Zugversuch und den Kennwerten aus den zyklischen, dehnungsgeregelten Versuchen ist grundsätzlich möglich. KW - Zyklische Werkstoffkennwerte KW - Zügige und zyklische Spannung-Dehnung-Kurve KW - Dehnungswöhlerlinie KW - Technischer Anriss KW - Elasto-plastisches Werkstoffverhalten KW - Automobilbau KW - Cyclic properties KW - Monotonic and cyclic stress-strain curve KW - Strain-life curve KW - Technical crack KW - Elasto-plastic material behaviour KW - Thin steel sheets KW - Automotive engineering KW - Karosseriestähle KW - Strukturen KW - Lebensdauerberechnung KW - Zyklische Kennwerte PY - 2004 DO - https://doi.org/10.1002/mawe.200400769 SN - 0933-5137 SN - 1521-4052 VL - 35 IS - 8 SP - 522 EP - 533 PB - Wiley-VCH Verl. CY - Weinheim AN - OPUS4-22436 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Steinbeck, G. A1 - Bleck, W. A1 - Bork, Claus-Peter A1 - Evertz, T. A1 - Frehn, A. A1 - Masendorf, R. A1 - Sonsino, C.M. ED - Palkowski, H. T1 - Ermittlung von Berechnungskennwerten an Karosseriestählen - Bericht über ein Gemeinschaftsprojekt der Stahl- und Automobilindustrie T2 - 4. Industriekolloquium - SFB 362 Fertigen in Feinblech - Werkstoffe Verfahren Konzepte CY - Bad Harzburg, Deutschland DA - 2004-02-11 KW - Karosseriestähle KW - Berechnung KW - Kennwerte PY - 2004 SN - 3937057056 VL - 1 IS - 2 SP - 127 EP - 136 PB - GRIPS media AN - OPUS4-22600 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - McMahon, Dino Peter A1 - Hayward, A. T1 - Why grow up? A perspective on insect strategies to avoid metamorphosis N2 - Insects with complete metamorphosis (holometaboly) are extremely successful, constituting over 60% of all described animal species. Complete metamorphosis confers significant advantages because it enables organisms to optimise life-history components through temporal partitioning, and thereby to exploitmultiple ecological niches. Yet holometaboly can also impose costs, and several lineages have evolved life cycle modifications to avoid complete metamorphosis. In this review, we discuss different strategies that have evolved that result in the loss of complete metamorphosis (type I and type II paedomorphosis). In addition, the ecological pressures and developmental modifications that facilitate this avoidance are considered, as well as the importance of life cycle complexity in life-history evolution. Interestingly, only female holometabolous insects have entirely avoided complete metamorphosis, and it is always the ancestrally juvenile morphology that is retained. These findings point to a strong sex-biased trade-off between investment in reproduction and development. While the loss of complete metamorphosis in females has occurred independently on several occasions across holometabolous insects, only a small number of species possessing this ability have been described. Thus, complete metamorphosis, which originated only once in insects, appears to have been almost fully retained. This indicates that significant modifications to the holometabolan metamorphic ground plan are highly constrained, and suggests that the transition to complete metamorphosis is evolutionarily irreversible. KW - Paedomorphosis; KW - Metamorphosis KW - Insect PY - 2016 DO - https://doi.org/10.1111/een.12313 SN - 0307-6946 SN - 1365-2311 VL - 41 IS - 5 SP - 505 EP - 515 AN - OPUS4-37851 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Heidt, Heinrich A1 - Goebbels, Jürgen A1 - Reimers, Peter A1 - Kettschau, A. ED - Aman, J. K. T1 - Development and application of an universal CAT-scanner T2 - 11th World Conference on Nondestructive Testing CY - Las Vegas, NV, USA DA - 1985-11-03 PY - 1985 SN - 0-931403-07-3 SP - 664 EP - 671 PB - Taylor Publ. CY - Dallas, Tex AN - OPUS4-34219 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Kettschau, A. A1 - Reimers, Peter A1 - Goebbels, Jürgen T1 - Computertomographie von radioaktiven Abfallgebinden T2 - Jahrestagung Kerntechnik CY - München, Germany DA - 1985-05-21 PY - 1985 SP - 423 EP - 426 PB - Deutsches Atomforum CY - Bonn AN - OPUS4-34220 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Kettschau, A. A1 - Reimers, Peter A1 - Goebbels, Jürgen A1 - Heidt, Heinrich T1 - Gamma-ray computed tomography of waste containers and conditioned radioactive waste in nuclear industry T2 - 7th International Conference on NDE in the nuclear industry CY - Grenoble, France DA - 1985-01-28 PY - 1985 SP - 321 EP - 324 CY - Grenoble AN - OPUS4-34221 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Oberpaul, M. A1 - Spohn, M. A1 - Fracowiak, J. A1 - Plarre, Rüdiger A1 - McMahon, Dino Peter A1 - Hammann, P. A1 - Vilcinskas, A. A1 - Gläser, J. T1 - Exploiting termite nest microbiomes for antibiotic discovery by using an ultra-high throughput Microfluidics/FACS driven pipeline combined with a microtiter plate based cultivation strategy N2 - Infections with multi-resistant Gram negative pathogens are a major threat to our health system. In order to serve the needs in antibiotics development we selected untapped bioresources and implemented high throughput approaches suitable for the discovery of strains producing antibiotics with anti-Gram negative activity. Our approaches rely on the hypothesis that Termite associated bacteria are likely to produce potent antibiotics to defend their hosts against entomopathogenic microorganisms. Termite nests and guts harbor suitable, highly diverse microbiomes in which bacterial taxa are present known to potentially produce natural compounds. In a first step the diversity of Coptotermes species nest microbiomes was assessed carefully by using 16S rDNA amplicon sequencing on the Illumina MiSeq platform and nest material was selected to retrieve viable cells by using Nycodenz density gradient centrifugation. In order to analyze the diversity of the culturable termite nest microbiome, bacterial cells were either distributed in 384-well plates (approach 1) or encapsulated in small spheric agarose beads by an high throughput microfluidics technique (approach 2). Cultures obtained from approach 1 were scaled-up in 96-well Duetz-systems for characterization of diversity and for rapid supernatant screening using the bioluminescence-labeled E. coli pFU166. The generated droplets of approach 2 simultaneously received a small population of GFP-tagged Gram negative screening cells and were sorted for low fluorescence using FACS. After elimination of redundancy we performed a fast scale-up of active strains. Implementation of this pipeline allows us to prioritize antibiotics producing strains in a ultra-high throughput fashion and by cultivation of broad diversity in our approches. T2 - Annual Conference of the Society for General and Applied Microbiology (VAAM) CY - Wolfsburg, Germany DA - 15.04.18 KW - Biotechnology KW - Termites KW - Anti-microbial effects PY - 2018 AN - OPUS4-44987 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Vogel, Christian A1 - Helfenstein, J. A1 - Massey, M. A1 - Sekine, R. A1 - Kretzschmar, R. A1 - Beiping, L. A1 - Peter, T. A1 - Chadwick, O. A1 - Tamburini, F. A1 - Rivard, C. A1 - Herzel, Hannes A1 - Adam, Christian A1 - Pradas del Real, A. A1 - Castillo-Michel, H. A1 - Zuin, L. A1 - Wang, D. A1 - Félix, R. A1 - Lassalle-Kaiser, B. A1 - Frossard, E. T1 - Microspectroscopy reveals dust-derived apatite grains in acidic, highly-weathered Hawaiian soils N2 - Dust deposition is an important source of phosphorus (P) to many ecosystems. However, there is little evidence of dust-derived P-containing minerals in soils. Here we studied P forms along a well-described climatic Gradient on Hawaii, which is also a dust deposition gradient. Soil mineralogy and soil P forms from six sites along the climatic gradient were analyzed with bulk (X-ray diffraction and P K-edge X-ray absorption near edge structure) and microscale (X-ray fluorescence, P K-edge X-ray absorption near edge structure, and Raman) analysis methods. In the wettest soils, apatite grains ranging from 5 to 30 μm in size were co-located at the micro-scale with quartz, a known continental dust indicator suggesting recent atmospheric deposition. In addition to co-location with quartz, further evidence of dust-derived P included backward trajectory modeling indicating that dust particles could be brought to Hawaii from the major global dust-loading areas in central Asia and northern Africa. Although it is not certain whether the individual observed apatite grains were derived from long-distance transport of dust, or from local dust sources such as volcanic ash or windblown fertilizer, these observations offer direct evidence that P-containing minerals have reached surface layers of highly-weathered grassland soils through atmospheric deposition. KW - Phosphorus KW - soil KW - microspectroscopy KW - Raman spectroscopy KW - XANES spectroscopy KW - x-ray diffraction PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-511522 DO - https://doi.org/10.1016/j.geoderma.2020.114681 SN - 0166-0918 SN - 1872-6259 VL - 381 SP - 114681-1 EP - 114681-11 PB - Elsevier CY - Amsterdam AN - OPUS4-51152 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Oberpaul, M. A1 - Zumkeller, C. M. A1 - Culver, T. A1 - Spohn, M. A1 - Mihajlovic1, S. A1 - Leis, B. A1 - Glaeser, S. P. A1 - Plarre, Rüdiger A1 - McMahon, Dino Peter A1 - Hammann, P. A1 - Schäberle, T. F. A1 - Glaeser, J. A1 - Vilcinskas, A. T1 - High-Throughput Cultivation for the Selective Isolation of Acidobacteria From Termite Nests N2 - Microbial communities in the immediate environment of socialized invertebrates can help to suppress pathogens, in part by synthesizing bioactive natural products. Here we characterized the core microbiomes of three termite species (genus Coptotermes) and their nest material to gain more insight into the diversity of termite-associated bacteria. Sampling a healthy termite colony over time implicated a consolidated and highly stable microbiome, pointing toward the fact that beneficial bacterial phyla play a major role in termite fitness. In contrast, there was a significant shift in the composition of the core microbiome in one nest during a fungal infection, affecting the abundance of wellcharacterized Streptomyces species (phylum Actinobacteria) as well as less-studied bacterial phyla such as Acidobacteria. High-throughput cultivation in microplates was implemented to isolate and identify these less-studied bacterial phylogenetic group. Amplicon sequencing confirmed that our method maintained the bacterial diversity of the environmental samples, enabling the isolation of novel Acidobacteriaceae and expanding the list of cultivated species to include two strains that may define new species within the genera Terracidiphilus and Acidobacterium. KW - Termite-associated microbes KW - Termites KW - Coptotermes KW - Core microbiome KW - Natural products discovery KW - Acidobacteria KW - underexplored phyla KW - Social insects PY - 2020 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-515283 DO - https://doi.org/10.3389/fmicb.2020.597628 VL - 11 SP - 1 EP - 16 AN - OPUS4-51528 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Bucek, A. A1 - Sobotnik, J. A1 - He, Shulin A1 - Shi, M. A1 - McMahon, Dino Peter A1 - Holmes, E.C. A1 - Roisin, Y. A1 - Lo, N. A1 - Bourguignon, T. T1 - Evolution of Termite Symbiosis Informed by Transcriptome-Based Phylogenies N2 - Termitidae comprises 80% of all termite species that play dominant decomposer roles in Tropical cosystems. Two major events during Termite evolution were the loss of cellulolytic gut protozoans in the ancestor of Termitidae and the subsequent gain in the termitid subfamily Macrotermitinae of fungal symbionts cultivated externally in ‘‘combs’’ constructed within the nest. How these symbiotic transitions occurred remains unresolved. Phylogenetic analyses of mitochondrial data previously suggested that Macrotermitinae is the earliest branching termitid lineage, followed soon after by Sphaerotermitinae, which cultivates bacterial symbionts on combs inside its nests. This has led to the hypothesis that comb building was an important evolutionary step in the loss of gut protozoa in ancestral termitids. We sequenced genomes and transcriptomes of 55 termite species and reconstructed phylogenetic trees from up to 4,065 orthologous genes of 68 species. We found strong support for a novel sister-group relationship between the bacterial comb-building Sphaerotermitinae and fungus comb-building Macrotermitinae. This key finding indicates that comb building is a derived trait within Termitidae and that the creation of a comb-like ‘‘external rumen’’ involving bacteria or fungi may not have driven the loss of protozoa from ancestral termitids, as previously hypothesized. Instead, associations with gut prokaryotic symbionts, combined with dietary shifts from wood to other plant-based substrates, may have played a more important role in this symbiotic transition. Our phylogenetic tree provides a platform for future studies of comparative termite evolution and the evolution of symbiosis in this taxon. KW - Molecular clock KW - Fungiculture KW - Gut symbionts KW - Insect evolution KW - Isoptera PY - 2019 DO - https://doi.org/10.1016/j.cub.2019.08.076 VL - 29 IS - 21 SP - 3728 EP - 3734.e4 PB - Elsevier Ltd. AN - OPUS4-49647 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - RPRT A1 - Hothan, Sascha A1 - Häßler, Dustin A1 - Fürst, Richard A1 - Peter, A. A1 - Wöhr, M. A1 - Rohleder, R. T1 - Entwicklung einer europäischen Prüfnorm zur Bestimmung des Feuerwiderstands von Stahlzuggliedern mit reaktiver Brandschutzbeschichtung anhand belasteter Brandprüfungen T1 - Development of a European test standard for the determination of the fire resistance of steel tension members with intumescent fire protection coating based on loaded fire tests N2 - Ziel des Forschungsvorhabens ist es, ein standardisiertes Prüf- und Bewertungsverfahren zur Bestimmung des Beitrags reaktiver Brandschutzsysteme (RBS) zum Feuerwiderstand von Stahlzuggliedern zu erarbeiten und dieses in ein Entwurfsdokument für eine europäische Prüfnorm zu überführen. Hierzu wurden einerseits bereits vorhandene Erfahrungen und Erkenntnisse genutzt und andererseits mittels Brandversuchen neue Prüfdaten zur Beantwortung bisher offengebliebener Aspekte zum Einfluss der Bauteilorientierung und Profilart erzeugt und ausgewertet. Dadurch wird die prüftechnische Grundlage für eine sichere, zulassungsbasierte Anwendung von reaktiven Brandschutzsystemen auf zugbeanspruchten Stahlbauteilen gelegt und ein einheitliches Sicherheitsniveau etabliert. Der im Rahmen des Forschungsprojektes entwickelte Normentwurf wurde mit den Fachexperten der zuständigen nationalen und europäischen Normungsgremien diskutiert und abgestimmt. Das erarbeitete Prüf- und Auswertungsverfahren basiert auf der Durchführung von Brandversuchen an mechanisch belasteten Stahlzuggliedern mit RBS. Ergänzend sind unbelastete Brandversuche zur Beurteilung des Einflusses der Bauteilorientierung sowie der Eignung bei Schwelbrandbeanspruchung möglich. Die im Projekt durchgeführten experimentellen Untersuchungen liefern die Grundlage für den im Normenentwurf vorgeschlagenen Prüfaufbau der mechanisch belasteten und unbelasteten Brandversuche. Ferner konnte durch die Untersuchungen gezeigt werden, dass sich unter bestimmten Voraussetzungen die Prüfergebnisse von Stahlzuggliedern mit Kreisvollprofil ohne zusätzliche Brandversuche auf entsprechende Zugglieder mit Rechteckvollprofil übertragen lassen. Darüber hinaus konnte belegt werden, dass die Leistungsfähigkeit eines RBS aufgrund unterschiedlicher Bauteilorientierungen abnehmen kann und daher zwingend beim Anwendungsbereich des RBS zu berücksichtigen ist. Hierzu wird ein Temperaturdifferenz-kriterium vorgeschlagen, bei dessen Einhaltung die Wirksamkeit des RBS bei von der horizontalen Referenzorientierung abweichenden Bauteilneigungen noch gleichwertig gegeben ist. Andernfalls bietet der Normenentwurf die Möglichkeit die reduzierte thermische Schutzwirkung des RBS im Assessment zu berücksichtigen. Die Ergebnisse aus dem Forschungsvorhaben wurden bereits auf einem wissenschaftlichen Symposium vorgestellt sowie in einer referierten Fachzeitschrift publiziert. Ferner wurden die Informationen zum Forschungsprojekt und dem Normenentwurf im Rahmen eines Anwenderworkshops interessierten Kreisen zugänglich gemacht. Der im Projekt ausgearbeitete Normenentwurf wird dem zuständigen europäischen Normungsgremien, CEN/TC 127/WG 1, zur Verfügung gestellt und dort zur Abstimmung gestellt. KW - Normung KW - Reaktives Brandschutzsystem KW - Stahl KW - Zugglied KW - Feuerwiderstand KW - Brandversuche PY - 2023 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-569363 SP - 1 EP - 57 PB - Bundesanstalt für Materialforschung und -prüfung (BAM) CY - Berlin AN - OPUS4-56936 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Dürig, Wiebke A1 - Lindblad, Sofia A1 - Golovko, Oksana A1 - Gkotsis, Georgios A1 - Aalizadeh, Reza A1 - Nika, Maria-Christina A1 - Thomaidis, Nikolaos A1 - Alygizakis, Nikiforos A. A1 - Plassmann, Merle A1 - Haglund, Peter A1 - Fu, Qiuguo A1 - Hollender, Juliane A1 - Chaker, Jade A1 - David, Arthur A1 - Kunkel, Uwe A1 - Macherius, André A1 - Belova, Lidia A1 - Poma, Giulia A1 - Preud'Homme, Hugues A1 - Munschy, Catherine A1 - Aminot, Yann A1 - Jaeger, Carsten A1 - Lisec, Jan A1 - Hansen, Martin A1 - Vorkamp, Katrin A1 - Zhu, Linyan A1 - Cappelli, Francesca A1 - Roscioli, Claudio A1 - Valsecchi, Sara A1 - Bagnati, Renzo A1 - González, Belén A1 - Prieto, Ailette A1 - Zuloaga, Olatz A1 - Gil-Solsona, Ruben A1 - Gago-Ferrero, Pablo A1 - Rodriguez-Mozaz, Sara A1 - Budzinski, Hélène A1 - Devier, Marie-Helene A1 - Dierkes, Georg A1 - Boulard, Lise A1 - Jacobs, Griet A1 - Voorspoels, Stefan A1 - Rüdel, Heinz A1 - Ahrens, Lutz T1 - What is in the fish? Collaborative trial in suspect and non-target screening of organic micropollutants using LC- and GC-HRMS N2 - A collaborative trial involving 16 participants from nine European countries was conducted within the NORMAN network in efforts to harmonise suspect and non-target screening of environmental contaminants in whole fish samples of bream (Abramis brama). Participants were provided with freeze-dried, homogenised fish samples from a contaminated and a reference site, extracts (spiked and non-spiked) and reference sample preparation protocols for liquid chromatography (LC) and gas chromatography (GC) coupled to high resolution mass spectrometry (HRMS). Participants extracted fish samples using their in-house sample preparation method and/or the protocol provided. Participants correctly identified 9-69% of spiked compounds using LC-HRMS and 20-60% of spiked compounds using GC-HRMS. From the contaminated site, suspect screening with participants’ own suspect lists led to putative identification of on average ~145 and ~20 unique features per participant using LC-HRMS and GC-HRMS, respectively, while non-target screening identified on average ~42 and ~56 unique features per participant using LC-HRMS and GC-HRMS, respectively. Within the same sub-group of sample preparation method, only a few features were identified by at least two participants in suspect screening (16 features using LC-HRMS, 0 features using GC-HRMS) and non-target screening (0 features using LC-HRMS, 2 features using GC-HRMS). The compounds identified had log octanol/water partition coefficient (KOW) values ranging from -9.9 to 16 and mass-to-charge ratio (m/z) of 68 to 761 (LC-HRMS and GC-HRMS). A significant linear trend was found between log KOW and m/z for the GC-HRMS data. Overall, these findings indicate that differences in screening results are mainly due to the data analysis workflows used by different participants. Further work is needed to harmonise the results obtained when applying suspect and non-target screening approaches to environmental biota samples. KW - General Environmental Science KW - Suspect and non-target analysis KW - Biota KW - Expobome KW - Collaborative trial PY - 2023 DO - https://doi.org/10.1016/j.envint.2023.108288 VL - 181 SP - 1 EP - 24 PB - Elsevier B.V. AN - OPUS4-58681 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Wilrich, Cordula A1 - Schröder, Volkmar A1 - Brandes, E. A1 - Schmidt, Martin A1 - Wandrey, Peter-Andreas A1 - Mix, K.-H. A1 - Wehrstedt, Klaus-Dieter A1 - Bender, H. A1 - Stephan, U. A1 - Eberz, A. A1 - Steinbach, J. A1 - Stoessel, F. T1 - Plant and process safety - 2. Hazardous materials and process conditions N2 - "Ullmann's Encyclopedia of Industrial Chemistry" is the benchmark reference in chemistry and chemical and life science engineering, covering inorganic and organic chemicals, advanced materials, pharmaceuticals, polymers and plastics, metals and alloys, biotechnology and biotechnological products, food chemistry, process engineering and unit operations, analytical methods, environmental protection, and much more. The present article is part of the topic collection "Environmental Protection and Industrial Safety". It covers plant and process safety, focusing on hazardous materials and process conditions. KW - Flammable gases and vapors KW - Combustibility of liquids KW - Dust explosions KW - Explosive condensed substances KW - Harmful effects of substances KW - Exothermic and pressure-inducing chemical reactions PY - 2012 SN - 978-3-527-30673-2 DO - https://doi.org/10.1002/14356007.q20_q01 SP - 1 EP - 50 PB - Wiley-VCH CY - Weinheim AN - OPUS4-58108 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Mazumdar, Tilomatta A1 - Bartholomäus, A. A1 - McMahon, Dino Peter ED - Rokas, A. T1 - Draft Genome of the Entomopathogenic Fungus Metarhizium robertsii DSM 1490 N2 - Metarhizium robertsii DSM 1490 is a generalist entomopathogenic fungus. The mechanisms of pathogenesis of such fungi in insects like termites are not completely understood. Here, we report the draft genome sequence, as sequenced on the Oxford Nanopore platform. The genome has a GC% of 47.82 and a size of 45,688,865 bp KW - Draft Genome KW - Entomopathogenic Fungus KW - Metarhizium robertsii DSM 1490 KW - Mechanisms of pathogenesis KW - Infecting a number of insects, KW - Close proximity to colonies PY - 2023 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-573186 DO - https://doi.org/10.1128/mra.01267-22 SN - 2576-098X VL - 2023 SP - 1 EP - 2 PB - ASM Journals CY - Nashville, USA AN - OPUS4-57318 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Vogel, K. A1 - Sieg, T. A1 - Veh, G. A1 - Fiedler, B. A1 - Moran, T. A1 - Peter, M. A1 - Rottler, E. A1 - Bronstert, A. T1 - Natural Hazards in a Changing World: Methods for Analyzing Trends and Non‐Linear Changes N2 - Estimating the frequency and magnitude of natural hazards largely hinges on stationary models, which do not account for changes in the climatological, hydrological, and geophysical baseline conditions. Using five diverse case studies encompassing various natural hazard types, we present advanced statistical and machine learning methods to analyze and model transient states from long‐term inventory data. A novel storminess metric reveals increasing European winter windstorm severity from 1950 to 2010. Non‐stationary extreme value models quantify trends, seasonal shifts, and regional differences in extreme precipitation for Germany between 1941 and 2021. Utilizing quantile sampling and empirical mode decomposition on 148 years of daily weather and discharge data in the European Alps, we assess the impacts of changing snow cover, precipitation, and anthropogenic river network modifications on river runoff. Moreover, a probabilistic framework estimates return periods of glacier lake outburst floods in the Himalayas, demonstrating large differences in 100‐year flood levels. Utilizing a Bayesian change point algorithm, we track the onset of increased seismicity in the southern central United States and find correlation with wastewater injections into deep wells. In conclusion, data science reveals transient states for very different natural hazard types, characterized by diverse forms of change, ranging from gradual trends to sudden change points and from altered seasonality to overall intensity variations. In synergy with the physical understanding of Earth science, we gain important new insights into the dynamics of the studied hazards and their possible mechanisms. KW - Natural hazards KW - Time dependent modeling KW - Transient state KW - Data science KW - Climate change PY - 2024 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-601540 DO - https://doi.org/10.1029/2023EF003553 VL - 12 IS - 5 SP - 1 EP - 29 PB - American Geophysical Union (AGU) AN - OPUS4-60154 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ramos, Inês I. A1 - Carl, Peter A1 - Schneider, Rudolf A1 - Segundo, Marcela A. A1 - Barreiros, Luisa A1 - Magalhaes, Luís M. A1 - Reis, Salette T1 - Development of automated immunosensing methods for clinical and environmental Analysis using lab-on-valve platforms N2 - The lab-on-valve (LOV) is a mesofluidic platform that has been recently exploited for the automation and miniaturization of bioanalytical assays, resorting namely to molecular recognition schemes based on immunosensing. Due to its high versatility for reagent accommodation, it is possible to establish immunoassays under several formats (eg. direct competitive ELISA, sandwich ELISA or even label-free immunoaffinity chromatography). For instance, the LOV has been used as a manifold for UV-vis micro-Bead Injection Spectroscopy (μ-BIS), a technique that involves the quantification of the target analyte by direct measurement on the surface of a solid phase capable of retaining the target analyte by molecular recognition. The μ-BIS-LOV strategy affords several analytical advantages, namely short time-toresult intervals (3 to 15 min), low sample volume (1-20 μL), automated solution handling and washing steps, downscaling of reagents’ consumption, low-cost analysis and little generation of waste. Additionally, the solid support is renewed before each determination, minimizing surface fouling, cross-contamination issues and functional group deactivation. No sample clean-up steps are required because interferences are separated from the target analyte upon quantification mediated by a molecular recognition element attached to the micro-bead column. The portability of the LOV device makes it compatible with point-of-care testing. To our knowledge, this technique has been mainly employed for the evaluation and optimization of bioaffinity processes, but its potential for clinical and environmental analysis remains underexploited. Hence, in this communication, different immunosensing strategies using the LOV platform will be addressed, namely the determination of autoimmune IgG in human serum, and the assessment of drug (carbamazepine) levels in wastewater samples. T2 - EBS 2017 CY - Potsdam, Germany DA - 20.03.2017 KW - Lab-on-valve KW - Automation KW - Immunoassay PY - 2017 AN - OPUS4-39521 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Carl, Peter A1 - Ramos, Inês I. A1 - Segundo, Marcela A. A1 - Schneider, Rudolf T1 - Antibody conjugation to carboxyl-modified microspheres through N-hydroxysuccinimide chemistry for automated immunoassay applications: A general procedure N2 - Immunochemical techniques are the workhorse for sample enrichment and detection of a large variety of analytes. In contrast to classical microtiter plate-based assays, microparticles are a next generation solid support, as they promote automation of immunoassays using flow-based techniques. Antibody immobilization is a crucial step, as these reagentsmare expensive, and inefficient coupling can result in low sensitivities. This paper proposes a general procedure for efficient immobilization of antibodies onto TentaGel particles, via Nhydroxysuccinimide chemistry. The goal was the preparation of solid supports with optimum immunorecognition, while increasing the sustainability of the process. The influence of buffer composition, activation and coupling time, as well as the amount of antibody on the immobilization efficiency was investigated, resorting to fluorophore-labeled proteins and fluorescence imaging. Buffer pH and activation time are the most important parameters for efficient coupling. It is demonstrated, that the hydrolysis of N-hydroxysuccinimide esters occurs at similar rates as in solution, limiting the utilizable time for coupling. Finally, applicability of the generated material for automated affinity extraction is demonstrated on the mesofluidic platform lab-on-valve. KW - Mesofluidics KW - Bead injections KW - ELISA KW - Carbamazepine KW - Biomarkers PY - 2019 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-483919 DO - https://doi.org/10.1371/journal.pone.0218686 SN - 1932-6203 VL - 14 IS - 6 SP - e0218686, 1 EP - 18 PB - Public Library of Science CY - San Francisco, California, USA AN - OPUS4-48391 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Ramos, I. I. A1 - Carl, Peter A1 - Schneider, Rudolf A1 - Segundo, M. A. T1 - Automated lab-on-valve sequential injection ELISA for determination of carbamazepine N2 - The development of an automated miniaturized analytical system that allows for the rapid monitoring of carbamazepine (CBZ) levels in serum and wastewater is proposed. Molecular recognition of CBZ was achieved through its selective interaction with microbeads carrying anti-CBZ antibodies. The proposed method combines the advantages of the micro-bead injection spectroscopy and of the flow-based platform lab-on-valve for implementation of automatic immunosorbent renewal, rendering a new recognition surface for each sample. The sequential (or simultaneous) perfusion of CBZ and the horseradish peroxidase-labelled CBZ through the microbeads is followed by real-time on-column Monitoring of substrate (3,30,5,50-tetramethylbenzidine) oxidation by colorimetry. The evaluation of the initial oxidation rate and also the absorbance value at a fixed time point provided a linear response versus the logarithm of the CBZ concentration. Under the selected assay conditions, a single analysis was completed after only 11 min, with a quantification range between 1.0 and 50 µg L⁻¹. Detection of CBZ levels in undiluted wastewater samples was feasible after a simple filtration step while good recoveries were attained for spiked certified human serum, analyzed without sample clean-up. KW - Automation KW - Bead injection spectroscopy KW - Human serum KW - Microparticles KW - Therapeutic Drug Monitoring KW - Wastewater PY - 2019 DO - https://doi.org/10.1016/j.aca.2019.05.017 SN - 0003-2670 VL - 1076 SP - 91 EP - 99 PB - Elsevier CY - Amsterdam AN - OPUS4-48317 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Dippong, Martin A1 - Carl, Peter A1 - Lenz, C. A1 - Schenk, J. A. A1 - Hoffmann, Katrin A1 - Schwaar, Timm A1 - Schneider, Rudolf A1 - Kuhne, Maren T1 - Hapten-specific single-cell selection of hybridoma clones by fluorescence-activated cell sorting for the generation of monoclonal antibodies N2 - The conventional hybridoma screening and subcloning process is generally considered to be one of the most critical steps in hapten-specific antibody production. It is time-consuming, monoclonality is not guaranteed, and the number of clones that can be screened is limited. Our approach employs a novel hapten-specific labeling technique of hybridoma cells. This allows for fluorescence-activated cell sorting (FACS) and single-cell deposition and thereby eliminates the above-mentioned problems. A two-step staining approach is used to detect antigen specificity and antibody expression: in order to detect antigen specificity, hybridoma cells are incubated with a hapten−horseradish peroxidase conjugate (hapten−HRP), which is subsequently incubated with a fluorophore-labeled polyclonal anti-peroxidase antibody (anti-HRP−Alexa Fluor 488). To characterize the expression of membrane-bound immunoglobulin G (IgG), a fluorophore-labeled anti-mouse IgG antibody (anti-IgG−Alexa Fluor 647) is used. Hundreds of labeled hybridoma cells producing monoclonal antibodies (mAbs) specific for a hapten were rapidly isolated and deposited from a fusion mixture as single-cell clones via FACS. Enzyme-linked immunosorbent assay (ELISA) measurements of the supernatants of the sorted hybridoma clones revealed that all hapten-specific hybridoma clones secrete antibodies against the target. There are significant improvements using this high-throughput technique for the generation of mAbs including increased yield of antibody-producing hybridoma clones, ensured monoclonality of sorted cells, and reduced development times. KW - Monoclonal antibodies KW - FACS KW - Hybridoma cells PY - 2017 DO - https://doi.org/10.1021/acs.analchem.6b04569 SN - 0003-2700 SN - 1520-6882 VL - 89 IS - 7 SP - 4007 EP - 4012 PB - ACS Publications AN - OPUS4-40320 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Riedel, Soraya A1 - Hülagü, Deniz A1 - Bennet, Francesca A1 - Carl, Peter A1 - Flemig, Sabine A1 - Schmid, Thomas A1 - Schenk, J. A. A1 - Hodoroaba, Vasile-Dan A1 - Schneider, Rudolf T1 - Electrochemical Immunomagnetic Ochratoxin A Sensing: Steps Forward in the Application of 3,3’,5,5’- Tetramethylbenzidine in Amperometric Assays N2 - Electrochemical methods offer great promise in meeting the demand for user-friendly on-site devices for Monitoring important parameters. The food industry often runs own lab procedures, for example, for mycotoxin analysis, but it is a major goal to simplify analysis, linking analytical methods with smart technologies. Enzyme-linked immunosorbent assays, with photometric detection of 3,3’,5,5’-tetramethylbenzidine (TMB),form a good basis for sensitive detection. To provide a straightforward approach for the miniaturization of the detectionstep, we have studied the pitfalls of the electrochemical TMB detection. By cyclic voltammetry it was found that the TMB electrochemistry is strongly dependent on the pH and the electrode material. A stable electrode response to TMB could be achieved at pH 1 on gold electrodes. We created a smartphonebased, electrochemical, immunomagnetic assay for the detection of ochratoxin A in real samples, providing a solid basis forsensing of further analytes. KW - Ochratoxin A KW - Amperometry KW - Cyclic voltammetry KW - Electrochemistry KW - Immunoassay PY - 2021 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-530421 DO - https://doi.org/10.1002/celc.202100446 N1 - Geburtsname von Riedel, Soraya: Höfs, S. - Birth name of Riedel, Soraya: Höfs, S. VL - 8 IS - 13 SP - 2597 EP - 2606 AN - OPUS4-53042 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ronen, A. A1 - Vogel, Christian A1 - Leube, Peter A1 - Ben Efraim, R. A1 - Nir, O. A1 - Chaudhary, M. A1 - Futterlieb, M. A1 - Panglisch, S. T1 - DEFEAT-PFAS: Detection, Quantification, and Treatment of Per- and Polyfluoroalkyl Substances (PFAS) in Groundwater N2 - The research project focuses on tackling the detection, measurement, and elimination of per- and polyfluoroalkyl substances (PFAS) from polluted groundwater, with a particular emphasis on addressing short (C4-C7) and ultrashort (C1-C3) chain PFAS. Given the widespread use of PFAS in various products, they are commonly found in groundwater near industrial and military sites in Germany and Israel. Moreover, recent regulations limiting the use of long chain PFAS have led industries to shift towards shorter chain alternatives. Hence, our efforts are geared towards refining detection, quantification, and removal methods for short and ultrashort chain PFAS. In terms of detection, ww are developing passive sampling devices capable of collecting and tracking the temporal distribution of PFAS species in groundwater. This will enable us to analyze contaminations in German and Israeli groundwater using cutting-edge analytical techniques. Additionally, contaminated groundwater will undergo a two-stage treatment process aimed at concentrating the relatively low PFAS concentrations using innovative membrane technologies such as closed-circuit reverse osmosis and mixed matrix composite nanofiltration membrane adsorbers. Subsequently, the streams containing higher PFAS concentrations will be treated through coagulation, with the remaining PFAS being adsorbed onto carbonaceous nanomaterials. The outcome of this research will include the creation of advanced tools for detecting, measuring, and eliminating PFAS from polluted groundwater, while also enhancing our understanding of the scope of these contaminations. T2 - German Israeli Water Technology Status Seminar CY - Koblenz, Germany DA - 18.06.2024 KW - Ground water KW - Per- and Polyfluoroalkyl substances (PFAS) KW - Remediation PY - 2024 UR - https://indico.scc.kit.edu/event/4029/attachments/7552/12045/Proceedings_Ger-Isr-Coop_Status-Seminar-2024.pdf SP - 33 EP - 36 AN - OPUS4-60331 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Czichocki, G. A1 - Heger, R. A1 - Much, Helmut A1 - Krüger, Ralph-Peter A1 - Goedel, W.A. T1 - Synthesis of Polyisoprenes with Sulfonate Head Groups and their Purification by HPLC PY - 2001 SN - 0932-3414 SN - 0040-3490 VL - 38 IS - 3 SP - 168 EP - 172 PB - Hanser CY - München AN - OPUS4-2175 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Tressl, R. A1 - Wondrak, G. T. A1 - Garbe, L.-A. A1 - Krüger, Ralph-Peter A1 - Rewicki, D. T1 - Pentoses and Hexoses as Sources of New Melanoidin-like Maillard-Polymers KW - Model compounds for melanoidins KW - Pyrroles from pentoses and hexoses KW - Beta-dicarbonyl pathway of the Maillard reaction KW - Polycondensation KW - MALDI-TOF-MS analysis KW - Antioxidative activity of oligomeric model compounds PY - 1998 SN - 0021-8561 SN - 1520-5118 VL - 46 IS - 5 SP - 1765 EP - 1776 PB - American Chemical Society CY - Columbus, Ohio AN - OPUS4-2159 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Dabrowski, A A1 - Robens, E. A1 - Klobes, Peter A1 - Meyer, Klaus A1 - Podkoscielny, P. T1 - Standardization of Methods for Characterizing the Surface Geometry of Solids N2 - Since a comprehensive survey published in 1999 [1] much work was done in standardizing measuring methods to characterize the surface geometry of dispersed and/or porous solids and to certify reference materials. The present paper is an extension of a short communication [2]. It gives a survey on existing standards and reports on new drafts and proposals. KW - Particle KW - Pore KW - Reference material KW - Standardization KW - Surface KW - Partikel KW - Poren KW - Oberfläche KW - Normung KW - Referenzmaterialien PY - 2003 DO - https://doi.org/10.1002/ppsc.200390037 SN - 0934-0866 SN - 1521-4117 VL - 20 IS - 5 SP - 311 EP - 322 PB - Wiley-VCH Verl. CY - Weinheim AN - OPUS4-5196 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Rouquerol, J. A1 - Baron, G. A1 - Denoyel, R. A1 - Giesche, H. A1 - Groen, J. A1 - Klobes, Peter A1 - Levitz, P. A1 - Neimark, A.V. A1 - Rigby, S. A1 - Skudas, R. A1 - Sing, K. A1 - Thommes, M. A1 - Unger, K. T1 - Liquid intrusion and alternative methods for the characterization of macroporous materials (IUPAC technical report) N2 - This document deals with the characterization of porous materials having pore widths in the macropore range of 50 nm to 500 µm. In recent years, the development of advanced adsorbents and catalysts (e.g., monoliths having hierarchical pore networks) has brought about a renewed interest in macropore structures. Mercury intrusion–extrusion porosimetry is a well-established method, which is at present the most widely used for determining the macropore size distribution. However, because of the reservations raised by the use of mercury, it is now evident that the principles involved in the application of mercury porosimetry require reappraisal and that alternative methods are worth being listed and evaluated. The reliability of mercury porosimetry is discussed in the first part of the report along with the conditions required for its safe use. Other procedures for macropore size analysis, which are critically examined, include the intrusion of other non-wetting liquids and certain wetting liquids, capillary condensation, liquid permeation, imaging, and image analysis. The statistical reconstruction of porous materials and the use of macroporous reference materials (RMs) are also examined. Finally, the future of macropore analysis is discussed. KW - Capillary condensation KW - Image analysis KW - IUPAC physical and biophysical chemistry division KW - Liquid intrusion KW - Macroporous materials KW - Mercury prorsimetry KW - Permeation KW - Pore size characterization KW - Reference porous materials KW - Statistical reconstruction of porous solids PY - 2012 DO - https://doi.org/10.1351/PAC-REP-10-11-19 SN - 0033-4545 SN - 1365-3075 VL - 84 IS - 1 SP - 107 EP - 136 PB - Union CY - Research Triangle Park, NC AN - OPUS4-26109 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Fürst, M.A. A1 - McMahon, Dino Peter A1 - Osborne, J.L. A1 - Paxton, R.J. A1 - Brown, M.J.F. T1 - Disease associations between honeybees and bumblebees as a threat to wild pollinators N2 - Emerging infectious diseases (EIDs) pose a risk to human welfare, both directly1 and indirectly, by affecting managed livestock and wildlife that provide valuable resources and ecosystem services, such as the pollination of crops2. Honeybees (Apis mellifera), the prevailing managed insect crop pollinator, suffer from a range of emerging and exotic high-impact pathogens3,4, and population maintenance requires active management by beekeepers to control them. Wild pollinators such as bumblebees (Bombus spp.) are in global decline5,6, one cause of which may be pathogen spillover from managed pollinators like honeybees7,8 or commercial colonies of bumblebees9. Here we use a combination of infection experiments and landscape-scale field data to show that honeybee EIDs are indeed widespread infectious agents within the pollinator assemblage. The prevalence of deformed wing virus (DWV) and the exotic parasite Nosema ceranae in honeybees and bumblebees is linked; as honeybees have higher DWV prevalence, and sympatric bumblebees and honeybees are infected by the same DWV strains, Apis is the likely source of at least one major EID in wild pollinators. Lessons learned from vertebrates10,11 highlight the need for increased pathogen control in managed bee species to maintain wild pollinators, as declines in native pollinators may be caused by interspecies pathogen transmission originating from managed pollinators. PY - 2014 DO - https://doi.org/10.1038/nature12977 SN - 0028-0836 SN - 1476-4687 VL - 506 SP - 364 EP - 366 PB - Nature Publishing Group CY - London AN - OPUS4-32884 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - McMahon, Dino Peter A1 - Fürst, M.A. A1 - Caspar, J. A1 - Theodorou, P. A1 - Brown, M.J.F. A1 - Paxton, R.J. T1 - A sting in the spit: widespread cross-infection of multiple RNA viruses across wild and managed bees N2 - 1.Declining populations of bee pollinators are a cause of concern, with major repercussions for biodiversity loss and food security. RNA viruses associated with honeybees represent a potential threat to other insect pollinators, but the extent of this threat is poorly understood. 2.This study aims to attain a detailed understanding of the current and ongoing risk of emerging infectious disease (EID) transmission between managed and wild pollinator species across a wide range of RNA viruses. 3.Within a structured large-scale national survey across 26 independent sites, we quantify the prevalence and pathogen loads of multiple RNA viruses in co-occurring managed honeybee (Apis mellifera) and wild bumblebee (Bombus spp.) populations. We then construct models that compare virus prevalence between wild and managed pollinators. 4.Multiple RNA viruses associated with honeybees are widespread in sympatric wild bumblebee populations. Virus prevalence in honeybees is a significant predictor of virus prevalence in bumblebees, but we remain cautious in speculating over the principle direction of pathogen transmission. We demonstrate species-specific differences in prevalence, indicating significant variation in disease susceptibility or tolerance. Pathogen loads within individual bumblebees may be high and in the case of at least one RNA virus, prevalence is higher in wild bumblebees than in managed honeybee populations. 5.Our findings indicate widespread transmission of RNA viruses between managed and wild bee pollinators, pointing to an interconnected network of potential disease pressures within and among pollinator species. In the context of the biodiversity crisis, our study emphasizes the importance of targeting a wide range of pathogens and defining host associations when considering potential drivers of population decline. KW - Apis KW - Bombus KW - Decline KW - Pathogen KW - Spillover PY - 2015 DO - https://doi.org/10.1111/1365-2656.12345 SN - 1365-2656 SN - 0021-8790 VL - 84 SP - 615 EP - 624 PB - Wiley-Blackwell CY - Oxford [u.a.] AN - OPUS4-32885 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - EvstigneevT, Roman V. A1 - Parfenov, Peter S. A1 - Dubavik, Aliaksei A1 - Cherevkov, Sergei A A1 - Fedorov, Anatoly V A1 - Martynenko, Irina V. A1 - Resch-Genger, Ute A1 - Ushakova, Elena V. A1 - Baranov, Alexander V. T1 - Time-resolved FRET in AgInS2/ZnS-CdSe/ZnS quantum dot systems N2 - The fast and accurate detection of disease-related biomarkers and potentially harmful analytes in different matrices is one of the main challenges in the life sciences. In order to achieve high signal-to-background ratios with frequently used photoluminescence techniques, luminescent reporters are required that are either excitable in the first diagnostic window or reveal luminescence lifetimes exceeding that of autofluorescent matrix components. Here, we demonstrate a reporter concept relying on broad band emissive ternary quantum dots (QDs) with luminescence lifetimes of a few hundred nanoseconds utilized for prolongating the lifetimes of organic or inorganic emitters with lifetimes in the order of a very few 10 ns or less through fluorescence resonant energy transfer. Using spectrally resolved and time-resolved measurements of the system optical response we demonstrate the potential of lifetime multiplexing with such systems exemplarily for AgInS2/ZnS and CdSe/ZnS QDs. KW - Nano KW - Nanomaterial KW - Ternary quantum dots KW - AIS KW - Semiconductor nanocrystal KW - Photoluminescence KW - Mechanism KW - Quantum yield KW - Photophysics KW - Energy transfer KW - Lifetime KW - Time-gated emission PY - 2019 DO - https://doi.org/10.1088/1361-6528/ab0136 SN - 0957-4484 SN - 1361-6528 VL - 30 IS - 19 SP - 195501, 1 EP - 7 PB - IOP Publishing Ltd AN - OPUS4-47434 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -