TY - GEN A1 - Haibel, A. A1 - Rack, A. A1 - Bütow, A. A1 - Kropf, H. A1 - Helwig, H.-M. A1 - Banhart, J. A1 - Weidemann, Gerd A1 - Riesemeier, Heinrich A1 - Goebbels, Jürgen T1 - Synchrotron-µ-tomography on complex materials PY - 2004 UR - http://www.helmholtz-berlin.de/media/media/oea/web/pr_webseite/druckschriften/berichte/bessy/annualreport2003.pdf#page=326 SN - 0179-4159 SP - 326 EP - 328 PB - Berliner Elektronenspeicherring-Gesellschaft für Synchrotronstrahlung CY - Berlin AN - OPUS4-3511 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Treimer, W. A1 - Feye–Treimer, U. A1 - Radke, C. A1 - Hilger, A. A1 - Neumann, O. A1 - Manke, I. A1 - Haibel, A. A1 - Rack, A. A1 - Riesemeier, Heinrich T1 - Investigations of mamma biopsies by means of µ-tomography PY - 2005 UR - http://www.helmholtz-berlin.de/media/media/oea/web/pr_webseite/druckschriften/berichte/bessy/annualreport2004.pdf#page=452 SN - 0179-4159 SP - 452 EP - 453 PB - Berliner Elektronenspeicherring-Gesellschaft für Synchrotronstrahlung CY - Berlin AN - OPUS4-7383 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Haibel, A. A1 - Rack, A. A1 - Weidemann, Gerd A1 - Riesemeier, Heinrich A1 - Goebbels, Jürgen A1 - Banhart, J. T1 - Investigation of pore formation in metallic foams by synchrotron computed tomography PY - 2004 UR - http://www.helmholtz-berlin.de/media/media/oea/web/pr_webseite/druckschriften/berichte/bessy/annualreport2003.pdf#page=324 SN - 0179-4159 SP - 324 EP - 325 PB - Berliner Elektronenspeicherring-Gesellschaft für Synchrotronstrahlung CY - Berlin AN - OPUS4-3510 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Weidemann, Gerd A1 - Goebbels, Jürgen A1 - Stadie, R. A1 - Hillemeier, B. A1 - Haibel, A. T1 - Untersuchung von faserverstärktem Porenbeton mit Computertomographie KW - Porenbeton KW - Faserorientierung KW - Computertomographie PY - 2007 SN - 978-3-931381-98-1 SP - 807 EP - 819 (Poster 22) PB - DGZfP CY - Berlin AN - OPUS4-15835 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Haibel, A. A1 - Rack, A. A1 - Weidemann, Gerd A1 - Riesemeier, Heinrich A1 - Goebbels, Jürgen A1 - Banhart, J. T1 - Synchrotron-m-Tomography on Aluminium and Zinc foams T2 - BESSY Users Meeting 2002 CY - Berlin, Germany DA - 2002-12-05 PY - 2002 AN - OPUS4-4934 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Zehbe, R. A1 - Haibel, A. A1 - Riesemeier, Heinrich A1 - Gross, U. A1 - Kirkpatrick, C.J. A1 - Schubert, H. A1 - Brochhausen, C. T1 - Going beyond histology. Synchrotron micro-computed tomography as a methodology for biological tissue characterization: from tissue morphology to individual cells N2 - Current light microscopic methods such as serial sectioning, confocal microscopy or multiphoton microscopy are severely limited in their ability to analyse rather opaque biological structures in three dimensions, while electron optical methods offer either a good three-dimensional topographic visualization (scanning electron microscopy) or high-resolution imaging of very thin samples (transmission electron microscopy). However, sample preparation commonly results in a significant alteration and the destruction of the three-dimensional integrity of the specimen. Depending on the selected photon energy, the interaction between X-rays and biological matter provides semi-transparency of the specimen, allowing penetration of even large specimens. Based on the projection-slice theorem, angular projections can be used for tomographic imaging. This method is well developed in medical and materials science for structure sizes down to several micrometres and is considered as being non-destructive. Achieving a spatial and structural resolution that is sufficient for the imaging of cells inside biological tissues is difficult due to several experimental conditions. A major problem that cannot be resolved with conventional X-ray sources are the low differences in density and absorption contrast of cells and the surrounding tissue. Therefore, X-ray monochromatization coupled with a sufficiently high photon flux and coherent beam properties are key requirements and currently only possible with synchrotron-produced X-rays. In this study, we report on the three-dimensional morphological characterization of articular cartilage using synchrotron-generated X-rays demonstrating the spatial distribution of single cells inside the tissue and their quantification, while comparing our findings to conventional histological techniques. KW - Cartilage KW - Chondrocyte KW - Synchrotron micro-computed tomography KW - Histology KW - Scanning electron microscopy KW - Three-dimensional imaging PY - 2010 DO - https://doi.org/10.1098/rsif.2008.0539 SN - 1742-5689 SN - 1742-5662 VL - 7 SP - 49 EP - 59 PB - The Royal Society CY - London AN - OPUS4-22123 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -