TY - CONF A1 - Schmidt, Stephan T1 - Monoclonal Antibodies against Diclofenac N2 - Mittels Festphasensynthese konnte ein Amid aus Diclofenac und 6-Aminohexansäure synthetisert werden. Dieses Hapten wurde an Trägerproteinen gekoppelt. Diese wurden für Immunisierungen und als Coatingantigene existierender Antikörper eingesetzt. Die Coatingantigene bewirkten eine Verbesserung etablierter indirekter ELISA mit kommerziell verfügbaren Antikörpern. Positive Immunantworten auf Diclofenac in der Immunisierung wurden bereits nachgewiesen. N2 - Diclofenac is a widely used drug against fever, inflammation, pain, and rheumatic diseases. An average of 70 % of the ingested diclofenac is excreted in the urine. Thus, 63 tons are introduced into the water cycle in Germany. Due to insufficient removal of diclofenac in wastewater treatment plants, residues of diclofenac can be found in surface water and sometimes in drinking water It exist a polyclonal against diclofenac and monoclonal antibodies against diclofenac and it`s metabolites. There all based on direct couling of diclofenac on proteins via carboxylic functions. However it was imagined advantageous to introduce a heterobifunctional spacer between the analyte and the carrier protein. Aceclofeac is commercial available. All other spacers can only be introduced with solid phase synthesis. T2 - Wasser 2016 CY - Bamberg, Germany DA - 02.05.2016 KW - Diclofenac KW - Proteinkonjugate KW - Haptensynthese KW - Monoklonale Antikörper KW - Monoclonal antibodies KW - Haptenes KW - Protein conjugates PY - 2016 AN - OPUS4-36707 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - RPRT A1 - Frost, K. A1 - Lüth, Peter A1 - Schmidt, Martin A1 - Simon, K. A1 - Uhlig, St. T1 - Evaluation of the interlaboratory test 2015-2016 on the method DIN EN 15188:2007 "Determination of the spontaneous ignition behaviour of dust accumulations" N2 - For the classification, safe handling and use of the chemicals, special standardized testing procedures have been developed and are used worldwide. Safety experts must be able to fully rely on the precise execution of the respective laboratory tests and assessments. In this context, interlaboratory tests are a crucial element of a laboratory's quality system. Participation in interlaboratory tests is explicitly recommended by the standard ISO/IEC 17025. The present document reports the results of the interlaboratory test 2015-2016, which was performed on the test method DIN EN 15188:2007 “Determination of the spontaneous ignition behaviour of dust accumulations”. It was organized by BAM in the frame of the co-operation project CEQAT-DGHS Centre for Quality Assurance for Testing of Dangerous Goods and Hazardous Substances. The test method DIN EN 15188:2007 is applied to characterize the self-ignition behaviour of combustible dusts. The experimental basis for describing the self-ignition behaviour of a given dust is the determination of the self-ignition temperatures (TSI) of differently-sized volumes of the dust sample by isoperibolic hot storage experiments (storage at constant oven temperatures) in commercially available ovens. The results measured this way reflect the dependence of the self-ignition temperatures on the volume of a dust accumulation. The interlaboratory test 2015-2016 on the method DIN EN 15188:2007 is the latest in a systematic stepwise built up series of method validation interlaboratory tests and internal laboratory investigations. The aim of this interlaboratory test was to determine measurement uncertainties of the modified method DIN EN 15188 for different substances, covering a sufficiently wide range of self-ignition behaviours in the scope of the DIN EN 15188 of the four basket test to extrapolate to storage volumes up to 1000 m³ and the single basket test for a basket volume of 1000 cm³. The precision of the four basket test and the single basket of the modified method DIN EN 15188 can be assessed as acceptable for the four sample materials investigated in the current interlaboratory test 2015-2016. It was possible to derive a functional equation for the measurement uncertainty U depending on the storage volume V. The measurement uncertainty cannot be ignored and must be considered, if TSI results should be used in practice. KW - Gefahrgut KW - Gefahrstoff KW - Ringversuch KW - Prüfmethode KW - Validierung KW - Qualitätssicherung KW - Messunsicherheit KW - Dangerous goods KW - Hazardous substances KW - Round robin test KW - Interlaboratory comparison KW - Test method KW - Validation KW - Measurement uncertainty PY - 2016 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-387343 SN - 978-3-9818270-0-2 SP - 1 EP - 106 PB - Bundesanstalt für Materialforschung und -prüfung (BAM) CY - Berlin AN - OPUS4-38734 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Haid, August A1 - Goetze, A1 - Selle, Hermann A1 - Koenen, Heinrich A1 - Becker, A1 - Schmidt, T1 - Sprengtechnische Untersuchungen - Die Detonationsfähigkeit von Gelatinedynamit in Abhängigkeit von der Lagerdauer PY - 1929 SN - 0368-1785 VL - VII SP - 174 EP - 175 PB - Verl. Chemie CY - Berlin AN - OPUS4-42142 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Kast, Hermann A1 - Haid, August A1 - Goetze, A1 - Selle, Hermann A1 - Metz, Ludwig A1 - Koenen, Heinrich A1 - Schmidt, T1 - Prüfungsmethoden - Versuche über den Wert und die Genauigkeit der Methylviolett- und Jodzinkstärkeprobe zur Beurteilung der chemischen Beständigkeit von rauchschwachem Pulver im Vergleich zu anderen Stabilitätsproben PY - 1929 SN - 0368-1785 VL - VI SP - 126 EP - 129 PB - Verl. Chemie CY - Berlin AN - OPUS4-43037 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Kast, Hermann A1 - Haid, August A1 - Goetze, A1 - Selle, Hermann A1 - Metz, Ludwig A1 - Koenen, Heinrich A1 - Schmidt, T1 - Prüfungsmethoden - Kritische Würdigung des sog. Abeltests zur Prüfung von Sprengstoffen auf chemische Beständigkeit PY - 1929 SN - 0368-1785 VL - VI SP - 129 EP - 132 PB - Verl. Chemie CY - Berlin AN - OPUS4-43039 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Kast, Hermann A1 - Haid, August A1 - Goetze, A1 - Selle, Hermann A1 - Metz, Ludwig A1 - Koenen, Heinrich A1 - Schmidt, T1 - Prüfungsmethoden - Prüfung der Beständigkeit von Sprengstoffen mit Hilfe von Wasserstoffionenmessungen PY - 1929 SN - 0368-1785 VL - VI SP - 132 EP - 133 PB - Verl. Chemie CY - Berlin AN - OPUS4-43040 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Kast, Hermann A1 - Haid, August A1 - Goetze, A1 - Selle, Hermann A1 - Metz, Ludwig A1 - Koenen, Heinrich A1 - Schmidt, T1 - Prüfungsmethoden - Bestimmung von Diphenylamin und Centralit in rauchschwachen Pulvern PY - 1929 SN - 0368-1785 VL - VI SP - 133 EP - 135 PB - Verl. Chemie CY - Berlin AN - OPUS4-43041 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Engl, T. A1 - Eberl, N. A1 - Gorse, C. A1 - Krüger, T. A1 - Schmidt, T. A1 - Plarre, Rüdiger A1 - Adler, C. A1 - Kaltenpoth, M. T1 - Ancient symbiosis confers desiccation resistance to stored grain pest beetles N2 - Microbial symbionts of insects provide a range of ecological traits to their hosts that are beneficial in the context of biotic interactions. However, little is known about insect symbiont-mediated adaptation to the abiotic environment, for example, temperature and humidity. Here, we report on an ancient clade of intracellular, bacteriome-located Bacteroidetes symbionts that are associated with grain and Wood pest beetles of the phylogenetically distant families Silvanidae and Bostrichidae. In the saw-toothed grain beetle Oryzaephilus surinamensis, we demonstrate that the symbionts affect cuticle thickness, melanization and hydrocarbon profile, enhancing desiccation resistance and thereby strongly improving fitness under dry conditions. Together with earlier observations on Symbiont contributions to cuticle biosynthesis in weevils, our findings indicate that convergent acquisitions of bacterial mutualists represented key adaptations enabling diverse pest beetle groups to survive and proliferate under the low ambient humidity that characterizes dry grain storage facilities. KW - Bacteroidetes KW - Cuticle KW - Desiccation resistance KW - Grain pest beetles KW - Symbiosis PY - 2017 DO - https://doi.org/10.1111/mec.14418 SN - 1365-294X SN - 0962-1083 VL - 27 IS - 8 SP - 2095 EP - 2108 AN - OPUS4-44013 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schmidt, Stephan A1 - Hoffmann, Holger A1 - Garbe, L.-A. A1 - Schneider, Rudolf T1 - Liquid chromatography-tandem mass spectrometry detection of diclofenac and related compounds in water samples N2 - A frequently studied environmental contaminant is the active substance diclofenac, which is removed insufficiently in sewage treatment plants. Since its inclusion in the watch list of the EU Water Framework Directive, the concentrations in surface waters will be determined throughout Europe. For this, still, more precise analytical methods are needed. As a reference, HPLC-MS is frequently employed. One of the major metabolites is 4’-hydroxydiclofenac (4’-OH-DCF). Also, diclofenac lactam is important for assessing degradation and transformation. Aceclofenac (ACF), the glycolic acid ester of diclofenac is used as a drug, too, and could potentially be cleaved to yield diclofenac again. In various sewage treatment plant influent samples, diclofenac, 4’-OH-DCF, DCF lactam and ACF could be determined with detection limits of 3 µg/L, 0.2 µg/L, 0.17 µg/L and 10 ng/L, respectively. KW - Diclofenac KW - Festphasenextraktion KW - HPLC-MS KW - Probenvorbereitung KW - Kläranlage KW - Abwasser KW - Zyklisierung KW - Diclofenac KW - Solid-phase extraction KW - Sewage treatment plant KW - Cyclization KW - Sample treatment KW - HPLC–MS PY - 2018 DO - https://doi.org/10.1016/j.chroma.2018.01.037 SN - 0021-9673 SN - 1873-3778 VL - 1538 SP - 112 EP - 116 PB - Elsevier B.V. CY - Amsterdam, NL AN - OPUS4-44183 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Haid, August A1 - Selle, Hermann A1 - Metz, Ludwig A1 - Koenen, Heinrich A1 - Schmidt, A1 - Becker, A1 - Hunold, A1 - Dittmar, Paul A1 - Frenzel, T1 - Arbeiten auf dem Gebiete der Sprengmittel - Bestimmung der Beständigkeit von Sprengstoffen PY - 1935 SN - 0368-1785 VL - XIV SP - 265 EP - 273 PB - Verl. Chemie CY - Berlin AN - OPUS4-43823 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -