TY - JOUR A1 - Fischer, J.L. A1 - Lutomski, C.A. A1 - El-Baba, T.J. A1 - Siriwardena-Mahanama, B.N. A1 - Weidner, Steffen A1 - Falkenhagen, Jana A1 - Allen, M. J. A1 - Trimpin, S. T1 - Matrix-assisted ionization-ion mobility spectrometry-mass spectrometry: Selective analysis of a europium-PEG complex in a crude mixture N2 - The analytical utility of a new and simple to use ionization method, matrix-assisted ionization (MAI), coupled with ion mobility spectrometry (IMS) and mass spectrometry (MS) is used to characterize a 2-armed europium(III)-containing poly(ethylene glycol) (Eu-PEG) complex directly from a crude sample. MAI was used with the matrix 1,2-dicyanobenzene, which affords low chemical background relative to matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI). MAI provides high ion abundance of desired products in comparison to ESI and MALDI. Inductively coupled plasma-MS measurements were used to estimate a maximum of 10% of the crude sample by mass was the 2-arm Eu-PEG complex, supporting evidence of selective ionization of Eu-PEG complexes using the new MAI matrix, 1,2-dicyanobenzene. Multiply charged ions formed in MAI enhance the IMS gas-phase separation, especially relative to the singly charged ions observed with MALDI. Individual components are cleanly separated and readily identified, allowing characterization of the 2-arm Eu-PEG conjugate from a mixture of the 1-arm Eu-PEG complex and unreacted starting materials. Size-exclusion chromatography, liquid chromatography at critical conditions, MALDI-MS, ESI-MS, and ESI-IMS-MS had difficulties with this analysis, or failed. KW - Matrix-assisted ionization ion mobility spectrometry mass spectrometry KW - Europium KW - Poly(ethylene glycol) KW - Size-exclusion chromatography KW - Liquid chromatography at critical conditions KW - Electrospray ionization KW - Matrix-assisted laser desorption/ionization KW - Inductively coupled plasma-mass spectrometry KW - ESI KW - MALDI KW - SEC PY - 2015 U6 - https://doi.org/10.1007/s13361-015-1233-8 SN - 1044-0305 VL - 26 IS - 12 SP - 2086 EP - 2095 PB - Elsevier CY - New York, NY AN - OPUS4-35283 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -