TY - CONF A1 - Wan, Wei A1 - Bell, Jérémy T1 - Use fluorogenic Molecularly imprinted particles to detect phosphate anion on the microfluidic chips T2 - 12. Dresdner Sensor-Symposium N2 - The exploration of new material and technique in the past half century has greatly improved our life quality but also brings threats like environmental pollution and desease. The monitoring of these harmful species is especially important for us human being and the planet we live. However, the traditional laboratory based assay is a complicate process including sample preparation, detection and data analysis. The onsite detection as well as point of care (POC) diagnostic are often limited by the dimension of large instrument and required trained people. In order to address these problems, we combined the sensor particle with the microfluidic chip technique for the potential miniaturized direct readout sensor instrument for onsite detection. MIPs are artificial receptors which are formed by polymerization of functional monomer at the presence of target molecule as template. They have comparable binding affinity and selectivity to the nature receptor such as anbibody and enzyme. However they are more stable and robust which greatly reduced the difficulty in storage and usage. The thermal and chemical stability also extends its application to more harsh conditions. Combined with core coding technique, they can also combine with fluidic assay for much higher throughout. We have developed a fluorogenic sensor particle which is formed by a thin fluorogenic molecularly imprinted polymer (fMIPs) shell of several nanometer coated onto a silica particle as core-substrate. These particles can respond to the molecule such as phosphorylated tyrosine through change of fluorescence signals and show high selectivity, sensitivity and fast response within 20 seconds. The phosphate anion is especially interesting due to its important role in industry as well as in biological processes. In order to make it applicable for on-site monitoring, we combined this fMIPs with a microfluidic chips. With carefully design, the particle can essentially mixed with the sample in the microfluidic chip. The excitation of sensor particle can be enabled by a LED, the fluorescence difference can then directly been registered with a spectrometer for qualification as well quantification the monitored species. The compact structure make the on-site as well as POC achievable. T2 - 12. Dresdner Sensor-Symposium CY - Dresden, Germany DA - 2015-12-07 PY - 2015 AN - OPUS4-35237 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Wan, Wei A1 - Rurack, Knut A1 - Biyikal, Mustafa A1 - Wagner, R. A1 - Sellergren, B. T1 - Direct Anion Sensing with Amplified Flourescence Using Moleculary Imprinted Core-Shell Nanoparticles T2 - 8. Deutsches BioSensor Symposium T2 - 8. Deutsches BioSensor Symposium CY - Wildau, Germany DA - 2013-03-10 PY - 2013 AN - OPUS4-27846 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Wan, Wei A1 - Rurack, Knut A1 - Biyikal, Mustafa A1 - Panne, Ulrich A1 - Wagner, R. A1 - Sellergren, B. T1 - Flourescent MIP sensors: broadening the scope of molecularly imprinted polymers T2 - ANAKON 2013 T2 - ANAKON 2013 CY - Essen, Germany DA - 2013-03-04 PY - 2013 AN - OPUS4-27849 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Wan, Wei A1 - Apel, Jenny A1 - Rurack, Knut A1 - Descalzo, Ana B. T1 - Assessing the FRET Acceptor Potential of Various BODIPY Dyes for Europium as the FRET Donor T2 - International Bunsen Discussion Meeting on Förster Resonance Energy Transfer in Life Sciences T2 - International Bunsen Discussion Meeting on Förster Resonance Energy Transfer in Life Sciences CY - Göttingen, Germany DA - 2011-03-27 PY - 2011 AN - OPUS4-23937 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Wan, Wei A1 - Biyikal, Mustafa A1 - Wagner, R. A1 - Sellergren, B. A1 - Rurack, Knut T1 - Hydrogen Bonding vs. Deprotonation: Binding of a Chromogenic Functional Monomer towards a Template in Molecular Imprinting Polymerization T2 - Affinity 2011 T2 - Affinity 2011 CY - Tavira, Portugal DA - 2011-06-16 PY - 2011 AN - OPUS4-24007 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -