TY - JOUR A1 - Shinde, S. A1 - El-Schich, Z. A1 - Malakpour, A. A1 - Wan, Wei A1 - Dizeyi, N. A1 - Mohammadi, R. A1 - Rurack, Knut A1 - Gjörloff Wingren, A. A1 - Sellergren, B. T1 - Sialic acid-imprinted fluorescent core-shell particles for selective labeling of cell surface glycans N2 - The expression of cell surface glycans terminating with sialic acid (SA) residues has been found to correlate with various disease states there among cancer. We here report a novel strategy for specific fluorescence labeling of such motifs. This is based on sialic acid-imprinted core–shell nanoparticles equipped with nitrobenzoxadiazole (NBD) fluorescent reporter groups allowing environmentally sensitive fluorescence detection at convenient excitation and emission wavelengths. Imprinting was achieved exploiting a hybrid approach combining reversible boronate ester formation between p-vinylphenylboronic acid and SA, the introduction of cationic amine functionalities, and the use of an NBD-appended urea-monomer as a binary hydrogen-bond donor targeting the SA carboxylic acid and OH functionalities. The monomers were grafted from 200 nm RAFT-modified silica core particles using ethylene glycol dimethacrylate (EGDMA) as cross-linker resulting in a shell thickness of ca. 10 nm. The particles displayed strong affinity for SA in methanol/water mixtures (K = 6.6 × 105 M-1 in 2% water, 5.9 × 103 M-1 in 98% water, Bmax ≈ 10 µmol g-1), whereas binding of the competitor glucuronic acid (GA) and other monosaccharides was considerably weaker (K (GA) = 1.8 × 103 M-1 in 98% water). In cell imaging experiments, the particles selectively stained different cell lines in correlation with the SA expression level. This was further verified by enzymatic cleavage of SA and by staining using a FITC labeled SA selective lectin. KW - Glycoproteine KW - Biomarker KW - Fluoreszenz KW - Polymere KW - Krebsdiagnostik PY - 2015 U6 - https://doi.org/10.1021/jacs.5b08482 SN - 0002-7863 SN - 1520-5126 VL - 137 IS - 43 SP - 13908 EP - 13912 PB - American Chemical Society CY - Washington, DC, USA AN - OPUS4-35227 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Wan, Wei A1 - Bell, Jérémy T1 - Use fluorogenic Molecularly imprinted particles to detect phosphate anion on the microfluidic chips N2 - The exploration of new material and technique in the past half century has greatly improved our life quality but also brings threats like environmental pollution and desease. The monitoring of these harmful species is especially important for us human being and the planet we live. However, the traditional laboratory based assay is a complicate process including sample preparation, detection and data analysis. The onsite detection as well as point of care (POC) diagnostic are often limited by the dimension of large instrument and required trained people. In order to address these problems, we combined the sensor particle with the microfluidic chip technique for the potential miniaturized direct readout sensor instrument for onsite detection. MIPs are artificial receptors which are formed by polymerization of functional monomer at the presence of target molecule as template. They have comparable binding affinity and selectivity to the nature receptor such as anbibody and enzyme. However they are more stable and robust which greatly reduced the difficulty in storage and usage. The thermal and chemical stability also extends its application to more harsh conditions. Combined with core coding technique, they can also combine with fluidic assay for much higher throughout. We have developed a fluorogenic sensor particle which is formed by a thin fluorogenic molecularly imprinted polymer (fMIPs) shell of several nanometer coated onto a silica particle as core-substrate. These particles can respond to the molecule such as phosphorylated tyrosine through change of fluorescence signals and show high selectivity, sensitivity and fast response within 20 seconds. The phosphate anion is especially interesting due to its important role in industry as well as in biological processes. In order to make it applicable for on-site monitoring, we combined this fMIPs with a microfluidic chips. With carefully design, the particle can essentially mixed with the sample in the microfluidic chip. The excitation of sensor particle can be enabled by a LED, the fluorescence difference can then directly been registered with a spectrometer for qualification as well quantification the monitored species. The compact structure make the on-site as well as POC achievable. T2 - 12. Dresdner Sensor-Symposium CY - Dresden, Germany DA - 2015-12-07 PY - 2015 AN - OPUS4-35237 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -