TY - JOUR A1 - Pisonero, J. A1 - Bouzas-Ramos, D. A1 - Traub, Heike A1 - Cappella, Brunero A1 - Álvarez-Llamas, C. A1 - Richter, Silke A1 - Mayo, J. C. A1 - Costa-Fernandez, J. M. A1 - Bordel, N. A1 - Jakubowski, Norbert T1 - Critical evaluation of fast and highly resolved elemental distribution in single cells using LA-ICP-SFMS N2 - The analytical potential of a nanosecond laser ablation coupled plasma mass spectrometer (ns-LA-ICP-SFMS)system is investigated for fast and highly spatially resolved (~µm) elemental distribution within single cells. The size, morphology and overlapping of laser-induced craters has been investigated with Atomic Force Microscopy (AFM). KW - Atomic Force Microscopy KW - Laser Ablation KW - Elemental Distribution PY - 2019 U6 - https://doi.org/10.1039/c8ja00096d SN - 0267-9477 VL - 34 IS - 4 SP - 655 EP - 663 PB - RSC AN - OPUS4-48549 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Pisonero, J. A1 - Traub, Heike A1 - Cappella, Brunero A1 - Álvarez-Llamas, C. A1 - Méndez, A. A1 - Richter, Silke A1 - Ruiz Encinar, J. A1 - Costa-Fernandez, J. M. A1 - Bordel, N. T1 - Exploring quantitative cellular biomaging and assessment of CdSe/ZnS quantum dots cellular uptake in single cells, using ns-LA-ICP-SFMS N2 - High spatially resolved quantitative bioimaging of CdSe/ZnS Quantum Dots uptake in two kinds of cells is investigated combining laser ablation inductively coupled plasma mass spectrometry and the spatially resolved analysis of dried pL-droplets from a solution with a known concentration of Quantum Dots. Single cells and dried pL-droplets are morphologically characterized by Atomic Force Microscopy. A number concentration of CdSe/ZnS QDs between 3.5 104 and 48 104 is estimated to be uptaken by several selected single cells, after being incubated in the presence of a QDs suspension added to a standard cell culture medium. Mono-elemental bioimaging at subcellular resolution seems to show a higher number concentration of the CdSe/ZnS QDs in the cytosol around the cell nucleus. KW - LA-ICP-SFMS KW - Fast single pulse response KW - Quantitative bioimaging KW - Cellular uptake KW - HT22 KW - HeLa KW - Single cell KW - pL-droplets KW - CdSe/ZnS quantum Dots KW - AFM PY - 2021 U6 - https://doi.org/10.1016/j.talanta.2021.122162 SN - 0039-9140 VL - 227 SP - 122162 PB - Elsevier B.V. AN - OPUS4-52121 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Piechotta, Christian A1 - Iznaguen, Hassan A1 - Traub, Heike A1 - Feldmann, Ines A1 - Köppen, Robert A1 - Witt, Angelika A1 - Jung, Christian A1 - Becker, Roland A1 - Oleszak, Katrin A1 - Bücker, Michael A1 - Urban, Klaus A1 - Reger, Christian A1 - Ostermann, Markus T1 - Bewitterungsszenarien im Vergleich – Veränderungen in der Oberflächenmorphologie von Polypropylen (PP) und Polystyrol (PS) unter dem Aspekt des Austrags von polybromierten Flammschutzmitteln N2 - Gegenstand der vorzustellenden Arbeiten ist die Prüfung der Umwelt-beständigkeit und -verträglichkeit von Materialien und Produkten hinsichtlich der Emission von potenziellen Schadstoffen in die Umwelt. Hierzu werden chemisch-physikalische Einflüsse (Bewitterung) und mikrobielle Beanspruchungen an Modellmaterialien evaluiert. So werden die Freisetzungsraten von Schadstoffen in Abhängigkeit der Beanspruchung beschrieben. Als Modellmaterialien kommen die Polymere Polystyrol (PS) und Polypropylen (PP) zum Einsatz. Synergistische Effekte der Bewitterungsparameter und der mikrobiologischen Beanspruchung sollen dabei ebenso betrachtet werden, wie die gezielte Alterung. Auch findet eine Beschreibung des Verhaltens der ausgetragenen Schadstoffe in den Umweltkompartimenten Boden oder Wasser statt. Hier sind mit Hilfe der zu entwickelnden Screening- und non-Target-Analyseverfahren die Transformation und der Metabolismus durch Mikroorganismen zu beschreiben. Aus den Ergebnissen sollen Korrelationen zwischen den künstlichen Alterungsverfahren und realen Szenarien abgeleitet werden. T2 - 47. Jahrestagung der GUS CY - Blankenloch-Stutensee, Germany DA - 21.03.2018 KW - Schadstoffaustrag KW - Umweltsimulation KW - Bewitterung PY - 2018 SN - 978-981-18507-2-7 VL - 47 SP - 115 EP - 128 AN - OPUS4-49802 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike A1 - Drescher, D. A1 - Büchner, T. A1 - Müller, L. A1 - Wanka, Antje A1 - Hösl, S. A1 - Ascher, Lena A1 - Cruz-Alonso, M. A1 - Pisonero, J. A1 - Kneipp, J. A1 - Jakubowski, Norbert T1 - Imaging of biological samples by LA-ICP-MS N2 - In recent years, elemental imaging of biological samples like tissue thin sections using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is gaining more and more importance. Improvements concerning spatial resolution as well as signal-to-background ratio due to low-dispersion sample chambers make LA-ICP-MS also interesting for single cell analysis. To evaluate the interaction of nanoparticles (NPs) with cells LA-ICP-MS was applied for the imaging of individual cells. Our findings show, that NP aggregates can be localized within cellular compartments. The uptake efficiency depends strongly on the physico-chemical properties of the nanostructures (size, chemical composition, surface modification), as well as on the incubation conditions (concentration, time). Moreover, LA-ICP-MS is increasingly becoming an important complementary technique in bioanalysis by using element-tagging strategies to determine biomolecules indirectly. Based on the specific binding between antibodies and their corresponding antigens, proteins and peptides can be detected in tissue or cells using tagged antibodies. As artificial tags metal chelates loaded with lanthanides, polymer-based elemental tags or metal-containing nanoparticles can be used. Thereby LA-ICP-MS is a sensitive detection tool for multiplexed immuno-histochemistry of tissue and cell samples. Our results demonstrate the potential of LA-ICP-MS to investigate the distribution of naturally occurring elements, administered agents as well as biomolecules by using metal-tagged antibodies. T2 - Workshop on tandem LIBS/LA-ICP-MS 2019 CY - Berlin, Germany DA - 18.11.2019 KW - Laser ablation KW - ICP-MS KW - Nanoparticles KW - Imaging PY - 2019 AN - OPUS4-49704 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Müller, L. A1 - Traub, Heike A1 - Jakubowski, Norbert T1 - Novel applications of lanthanoides as analytical or diagnostic tools in the life sciences by ICP-MS-based techniques N2 - Inductively coupled plasma mass spectrometry (ICP-MS) is a well-established analytical method for multi-elemental analysis in particular for elements at trace and ultra-trace levels. It has found acceptance in various application areas during the last decade. ICP-MS is also more and more applied for detection in the life sciences. For these applications, ICP-MS excels by a high sensitivity, which is independent of the molecular structure of the analyte, a wide linear dynamic range and by excellent multi-element capabilities. Furthermore, methods based on ICP-MS offer simple quantification concepts, for which usually (liquid) standards are applied, low matrix effects compared to other conventional bioanalytical techniques, and relative limits of detection (LODs) in the low pg g−1 range and absolute LODs down to the attomol range. In this chapter, we focus on new applications where the multi-element capability of ICP-MS is used for detection of lanthanoides or rare earth elements, which are applied as elemental stains or tags of biomolecules and in particular of antibodies. KW - ICP-MS KW - Life sciences KW - Mass cytometry KW - Laser ablation (LA)-ICP-MS KW - Bioimaging PY - 2016 U6 - https://doi.org/10.1515/psr-2016-0064 SN - 2365-659X SN - 2365-6581 VL - 1 IS - 11 SP - 1 EP - 19 AN - OPUS4-40234 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CHAP A1 - Müller, L. A1 - Traub, Heike A1 - Jakubowski, Norbert ED - Prof. Dr. Golloch, Alfred T1 - Novel applications of lanthanoides as analytical or diagnostic tools in the life sciences by ICP-MS-based techniques N2 - Inductively coupled plasma mass spectrometry (ICP-MS) is a well-established analytical method for multi-elemental analysis in particular for elements at trace and ultra-trace levels. It has found acceptance in various application areas during the last decade. ICP-MS is also more and more applied for detection in the life sciences. For these applications, ICP-MS excels by a high sensitivity, which is independent of the molecular structure of the analyte, a wide linear dynamic range and by excellent multi-element capabilities. Furthermore, methods based on ICP-MS offer simple quantification concepts, for which usually (liquid) standards are applied, low matrix effects compared to other conventional bioanalytical techniques, and relative limits of detection (LODs) in the low pg g−1 range and absolute LODs down to the attomol range. In this chapter, we focus on new applications where the multi-element capability of ICP-MS is used for detection of lanthanoides or rare earth elements, which are applied as elemental stains or tags of biomolecules and in particular of antibodies. KW - ICP-MS KW - Life sciences KW - Mass cytometry KW - Laser ablation (LA)-ICP-MS KW - Bioimaging PY - 2017 SN - 978-3-11-036523-8 SP - Chapter 11, 301 EP - 320 PB - De Gruyter AN - OPUS4-40244 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Esteban-Fernandez, Diego A1 - Traub, Heike A1 - Hutchinson, R. A1 - Jakubowski, Norbert A1 - Wilkins, J. A1 - Summerfield, L. A1 - McLachlin, K. T1 - High resolution laser ablation NWRimage system for single cell imaging N2 - The traceability and availability of nanoparticles enables their use to enhance a variety of nano-biological and nano-medicinal applications. The particular size and shape of nanoparticles determine the uptake rate and pathway into the cell, and therefore impact specific cell components and processes. Selecting specific particle types allows researchers to target the process or structure of interest, with minimal additional impact. This can be used for drug or DNA delivery, and is being explored for use in oncology. Understanding the different uptake mechanisms and impacted processes requires sub-cellular Imaging resolution to determine, for example, whether or not the nanoparticles are reaching the nucleus. Sub-cellular imaging has traditionally been challenging to achieve with laser ablation ICP-MS due to a lack of sensitivity at small spots. Bioimaging using LA-ICP-MS is a well-established technique, but usually applied on the tissue scale, which depends on larger spot areas where sensitivity is less problematic. The improved sensitivity and washout from the NWRimage has allowed faster imaging of smaller spots. The NWRimage also provides the possibility of true sub-micron spot sizes. This work compares the capabilities of standard laser Ablation (NWR213 system) with results from the NWRimage platform, which has been optimized for imaging applications. T2 - European Winter Conference on Plasma Spectrochemistry CY - St. Anton, Austria DA - 19.02.2017 KW - Laser ablation KW - Imaging KW - Nanoparticle PY - 2017 AN - OPUS4-39281 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike A1 - Büchner, T. A1 - Drescher, D. A1 - Merk, V. A1 - Kneipp, J. A1 - Jakubowski, Norbert T1 - Studying nanoparticle-cell interaction by ICP-MS based techniques N2 - Nanoparticles (NPs) have found a wide range of applications in research and industry. Thereby the interaction of NPs with biological systems like cells has become a major field of interest, ranging from medical applications to nanotoxicology. Size, shape and surface modification of the nanomaterials determine the uptake rate and pathway into the cells, and therefore impact specific cell components and processes. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is an established quantitative multi-elemental analysis and mapping technique. In recent years, it was shown that LA-ICP-MS can provide quantitative as well as distribution information of metal containing nanoparticles (NPs) in cell samples. Here LA-ICP-MS was applied for the imaging of individual fibroblast cells to study the uptake and intracellular processing of NPs. Our results show that LA-ICP-MS can be used to localize nanoparticle aggregates within cellular compartments. The studied NPs accumulate in the perinuclear region in the course of intracellular processing, but do not enter the cell nucleus. The uptake efficiency depends strongly on the physico-chemical properties of the nanostructures as well as on the incubation conditions like concentration and incubation time. ICP-MS was used to determine the composition of the nanomaterials as well as the number of NPs in cells after acid digestion of the samples. T2 - 13. Symposium „Massenspektrometrische Verfahren der Element­spurenanalyse“ & 26. ICP-MS-Anwendertreffen CY - Berlin, Germany DA - 03.09.2018 KW - ICP-MS KW - Nanoparticle KW - Cell KW - Laser ablation PY - 2018 AN - OPUS4-45860 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike A1 - Drescher, D. A1 - Büchner, T. A1 - Zeise, Ingrid A1 - Kneipp, Janina A1 - Jakubowski, Norbert T1 - Imaging of nanoparticles in cells by LA-ICP-MS N2 - The interaction of nanoparticles (NPs) with cells has become a major field of interest, ranging from therapeutic applications to nanotoxicology. The cellular uptake depends on the primary characteristics of the NPs (e.g. size, shape, surface modification) and on the cells interacting with the particles. Thereby the quantification of NPs in cells is of particular importance to obtain information under different experimental conditions. Presently, the number of NPs internalized is often determined by inductively coupled plasma (ICP) optical emission spectrometry (OES) or ICP mass spectrometry (MS) after acid digestion of a cell suspension or a cell pellet. The result is an average value and no information about the distribution among cells or within a cell is available. Therefore we developed a method based on laser ablation (LA) in combination with ICP-MS to localise and quantify metallic NPs in single cells. LA-ICP-MS is a powerful analytical method which offers excellent sensitivity at high spatial resolution and multielement capability without time-consuming sample preparation steps. Recently, LA-ICP-MS was established for elemental mapping of biological samples like tissues. In our experiments, fibroblast cells were incubated with gold or silver containing nanoparticles and grown on sterile coverslips under standard conditions. For LA analysis the cells were fixed with formaldehyde and dried. Subcellular resolution is achived by careful optimisation of laser energy, ablation frequency and scan speed. The elemental distribution was determined by continuous ablation line by line of cells incubated with NPs. Our results show that LA-ICP-MS is able to detect NP aggregates within cellular substructures. After 24 h of incubation the NPs were found in the cytosol, preferencially in the perinuclear region, but do not enter the nucleus. Additionally, a quantification strategy at single-cell level was developed. For this purpose nitrocellulose membrane was spiked with Ag or Au nanoparticle suspension at different concentration levels and analysed by LA-ICP-MS. Based on this calibration the number of NPs taken up by individual cells was determined and variations within the cell population become visible. The cells show a strong dependence of NP uptake on concentration and incubation time. Our results demonstrate the potential of LA-ICP-MS providing insight into NP uptake and intracellular distribution dependent on experimental parameters. T2 - Erfahrungsaustausch LA-ICP-MS mit Bayer AG CY - Berlin, Germany DA - 11.09.2017 KW - LA-ICP-MS KW - Bioimaging KW - Nanoparticles KW - Cell PY - 2017 AN - OPUS4-42575 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Phukphatthanachai, Pranee A1 - Vogl, Jochen A1 - Traub, Heike A1 - Jakubowski, Norbert A1 - Panne, Ulrich T1 - A new approach of using polyethylene frits for the quantification of sulphur in copper metals by isotope dilution LA-ICP-MS and comparison with conventional IDMS techniques N2 - Polyethylene (PE) frits were used to quantify sulphur in copper and its alloys by isotope dilution combined with LA-ICP-MS as an alternative approach to conventional sample preparation: the copper samples were spiked, the spiked samples were dissolved, the resulting solutions were absorbed in the PE frits and finally the PE frits were analysed by LA-ICP-MS. A prerequisite for such a support material is a low sulphur blank and thus PE was selected for this purpose. The absorption efficiency of the PE frits was studied for varying sulphur amounts ranging from 2 mg S to 80 mg S showing that more than 99.5% of the loaded sulphur was absorbed by the frit. The so prepared PE frits were measured by LA-ICP-MS and yielded a good linearity (R2 ¼ 0.999) for the sulphur ion intensities corresponding to sulphur amounts up to 40 mg S; the associated sensitivity is approximately 3.4 x 10⁴ cps μg⁻¹ for ³²S. For the validation of the developed procedure the reference materials BAM-M376a, BAM-228 and BAM-227 were applied such that 2 μg S, 5 μg S and 11 μg S were absorbed in the PE frits, respectively. These samples were pre-quantified for the adsorbed sulphur amount by external calibration LA-ICP-MS yielding sulphur amounts of 0.9 μg, 5.1 μg and 8.5 μg (quantified for ³²S only), respectively. Relative Standard deviations of the isotope ratios were below 5% in average (n ¼ 3 lines) in all cases (except for the pure spike solution). These samples were then analysed by LA-ICP-IDMS and the measurement results were validated by comparing them with the results obtained by conventional ICP-IDMS. The obtained relative expanded measurement uncertainties ranged between 10% and 26%. Pearson's coefficient was used to express the correlation between both techniques; the obtained value was 0.999 demonstrating a strong correlation. Contrary to most published LA-ICP-IDMS procedures, the developed procedure enables SI-traceability for the measurement results. The metrological traceability to the SI for the sulphur mass fractions in copper was established by an unbroken chain of comparisons, each accompanied by an uncertainty budget. Thus, the measurement results are considered reliable, acceptable and comparable within the stated measurement uncertainty. The metrological traceability chain from the kg down to mass fraction in the samples obtained by LA-ICP-IDMS is presented as well. KW - Laser ablation KW - IDMS KW - Traceability KW - Uncertainty KW - Purity PY - 2018 U6 - https://doi.org/10.1039/c8ja00116b SN - 0267-9477 SN - 1364-5544 VL - 33 IS - 9 SP - 1506 EP - 1517 PB - Royal Society of Chemistry CY - Cambridge AN - OPUS4-45899 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -