TY - CONF A1 - Traub, Heike T1 - Determination of trace elements in Nb2O5 powder using LA-ICP-MS T2 - 10th European Workshop on Laser Ablation CY - Kiel, Germany DA - 2010-06-29 PY - 2010 AN - OPUS4-21790 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Determination of trace impurities in pure copper using LA-ICP-MS T2 - 8th European Workshop on Laser Ablation in Elemental Analysis CY - Zurich, Switzerland DA - 2006-07-19 PY - 2006 AN - OPUS4-12575 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Analyse von Reinkupfer mit LA-ICP-MS: Vergleich unterschiedlicher Kalibrierstrategien T2 - ANAKON 2009 CY - Berlin, Germany DA - 2009-03-17 PY - 2009 AN - OPUS4-19009 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Laser ablation ICP-MS for the determination of trace impurities in pure copper T2 - IGAS-Jahreskolloquium 2007 CY - Berlin, Germany DA - 2007-11-08 PY - 2007 AN - OPUS4-16304 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Traub, Heike A1 - Wälle, M. A1 - Koch, J. A1 - Panne, Ulrich A1 - Matschat, Ralf A1 - Kipphardt, Heinrich A1 - Günther, D. T1 - Evaluation of different calibration strategies for the analysis of pure copper and zinc samples using femtosecond laser ablation ICP-MS N2 - Solution-doped metal powder pellets as well as aspirated liquids were used as calibration samples to analyze pure copper and zinc certified reference materials (CRMs) by femtosecond laser ablation ICP-MS. It was demonstrated that calibration by copper pellets resulted in relative deviations up to 20%, whereas fs-LA-ICP-MS among copper-based CRMs led to inaccuracies in the same range unless nominal mass fractions were chosen to be <3 mg/kg. Calibration by zinc pellets generally provided better accuracy. Depending on the analyte considered, deviations below 10% were obtained even for mass fractions close to the limit of quantification. Our data, therefore, indicate solution-doped metal powder pellets to be suitable as calibration samples for fs-LA-ICP-MS of metals. Furthermore, the utilization of liquid standards for calibration was found to result in stronger deviations of up to 50% for both copper and zinc samples which, in addition, turned out to be dependent on the plasma conditions. KW - Laser ablation KW - ICP-MS KW - Calibration KW - Copper KW - Zinc KW - Metal PY - 2009 DO - https://doi.org/10.1007/s00216-009-3061-9 SN - 1618-2642 SN - 1618-2650 VL - 395 IS - 5 SP - 1471 EP - 1480 PB - Springer CY - Berlin AN - OPUS4-20402 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Traub, Heike A1 - Czerwensky, Michael A1 - Matschat, Ralf A1 - Kipphardt, Heinrich A1 - Panne, Ulrich T1 - Different calibration strategies for the analysis of pure copper metal by nanosecond laser ablation inductively coupled plasma mass spectrometry N2 - In this work, different calibration strategies for the determination of trace elements in pure copper metal by nanosecond laser ablation ICP-MS were investigated. In addition to certified reference materials (CRMs), pellets of doped copper powder were used for calibration. The micro homogeneity of the CRMs as well as the solution-doped pellets was sufficient to use them as calibration samples in combination with a laser spot size of 200 µm. In contrast, pellets doped with analytes in solid form showed a significant heterogeneity. For most of the investigated analytes and copper CRMs the measured mass fractions were within ± 20% of their certified values when other copper CRMs were used as calibration samples. When solution-doped powder pellets were used as calibration samples a systematic trend towards mass fractions below the certified values was observed for nearly all elements determined in the analysed CRMs. Thermal fractionation effects during the ablation of the solution-doped pellets were suspected as the extent of the fractionation depends on the irradiance, whereas fractionation is reduced at higher irradiance. PY - 2010 DO - https://doi.org/10.1039/b915564c SN - 0267-9477 SN - 1364-5544 VL - 25 IS - 5 SP - 690 EP - 696 PB - Royal Society of Chemistry CY - London AN - OPUS4-21195 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Jakubowski, Norbert A1 - Müller, L. A1 - Traub, Heike A1 - Esteban-Fernández, D. A1 - Panne, Ulrich A1 - Herrmann, Antje A1 - Schellenberger, E. A1 - Theuring, F. A1 - Kneipp, Janina T1 - Imaging by laser ablation ICP-MS N2 - ICP-MS is a well-established analytical method which excels by high accuracy, high dynamic range and extremely low limits of detection for most metals. Furthermore ICP-MS offers a very high multi-element coverage so that many elements of the periodic table can be detected simultaneously. In this series of lectures, we want to focus on the historical developments, fundamentals, instrumentation and novel applications of ICP-MS in the life and material sciences. T2 - Ringvorlesung Analytik CY - Humboldt-Universität zu Berlin DA - 23.06.2017 KW - Laser ablation ICP-MS KW - Bio-Imaging PY - 2017 AN - OPUS4-40757 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Wienold, Julia A1 - Traub, Heike A1 - Bresch, Harald A1 - Seeger, Stefan A1 - Recknagel, Sebastian A1 - Kipphardt, Heinrich T1 - Effort on calibration of infrared spark ablation of copper with synthetic copper standards N2 - Two types of copper samples, compact certified copper reference materials and calibration samples prepared from liquid doped, pressed copper powders, were studied in terms of accuracy of obtained calibration functions originating from infrared spark ablation. Additionally, corresponding particle size distributions of the aerosols from infrared spark ablation were recorded. It is shown that the differences in quantification results, originating from the two sets of calibration functions, could not mainly be ascribed to different particle size distributions of the two copper sample types. Possible other causes, as different ablation rates, parts of melting and differences of the chemical constitutions of the two sample types were explored. KW - Calibration KW - Spark ablation KW - Synthetic standard KW - Particle size distribution KW - Fractionation KW - Laser ablation KW - ICP-MS PY - 2011 DO - https://doi.org/10.1016/j.sab.2011.04.005 SN - 0584-8547 SN - 0038-6987 VL - 66 IS - 6 SP - 432 EP - 438 PB - Elsevier B.V. CY - Amsterdam AN - OPUS4-24065 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Localisation and quantification of metallic nanoparticles in single cells by LA-ICP-MS T2 - 12th European Workshop on Laser Ablation (EWLA2014) CY - London, England DA - 2014-07-08 PY - 2014 AN - OPUS4-31099 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Drescher, Daniela A1 - Zeise, Ingrid A1 - Traub, Heike A1 - Guttmann, P. A1 - Seifert, Stephan A1 - Büchner, Tina A1 - Jakubowski, Norbert A1 - Schneider, G. A1 - Kneipp, Janina T1 - In situ characterization of SiO2 nanoparticle biointeractions using BrightSilica N2 - By adding a gold core to silica nanoparticles (BrightSilica), silica-like nanoparticles are generated that, unlike unmodified silica nanoparticles, provide three types of complementary information to investigate the silica nano-biointeraction inside eukaryotic cells in situ. Firstly, organic molecules in proximity of and penetrating into the silica shell in live cells are monitored by surface-enhanced Raman scattering (SERS). The SERS data show interaction of the hybrid silica particles with tyrosine, cysteine and phenylalanine side chains of adsorbed proteins. Composition of the biomolecular corona of BrightSilica nanoparticles differs in fibroblast and macrophage cells. Secondly, quantification of the BrightSilica nanoparticles using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) micromapping indicates a different interaction of silica nanoparticles compared to gold nanoparticles under the same experimental conditions. Thirdly, the metal cores allow the investigation of particle distribution and interaction in the cellular ultrastructure by cryo nanoscale X-ray tomography (cryo-XT). In 3D reconstructions the assumption is confirmed that BrightSilica nanoparticles enter cells by an endocytotic mechanism. The high SERS intensities are explained by the beneficial plasmonic properties due to agglomeration of BrightSilica. The results have implications for the development of multi-modal qualitative and quantitative characterization in comparative nanotoxicology and bionanotechnology. KW - Silica nanoparticles KW - Surface-enhanced Raman scattering KW - X-ray tomography KW - LA-ICP-MS KW - Core–shell structures PY - 2014 DO - https://doi.org/10.1002/adfm.201304126 SN - 1616-301X SN - 1616-3028 VL - 24 IS - 24 SP - 3765 EP - 3775 PB - Wiley-VCH CY - Weinheim AN - OPUS4-30924 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Localisation and quantification of metallic nanoparticles in single cells by laser ablation ICP-MS T2 - NanoBio Europe, 10th International Congress & Exhibition on Nanobiotechnology CY - Münster, Germany DA - 2014-06-02 PY - 2014 AN - OPUS4-30781 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Müller, L. A1 - Traub, Heike A1 - Jakubowski, Norbert T1 - Novel applications of lanthanoides as analytical or diagnostic tools in the life sciences by ICP-MS-based techniques N2 - Inductively coupled plasma mass spectrometry (ICP-MS) is a well-established analytical method for multi-elemental analysis in particular for elements at trace and ultra-trace levels. It has found acceptance in various application areas during the last decade. ICP-MS is also more and more applied for detection in the life sciences. For these applications, ICP-MS excels by a high sensitivity, which is independent of the molecular structure of the analyte, a wide linear dynamic range and by excellent multi-element capabilities. Furthermore, methods based on ICP-MS offer simple quantification concepts, for which usually (liquid) standards are applied, low matrix effects compared to other conventional bioanalytical techniques, and relative limits of detection (LODs) in the low pg g−1 range and absolute LODs down to the attomol range. In this chapter, we focus on new applications where the multi-element capability of ICP-MS is used for detection of lanthanoides or rare earth elements, which are applied as elemental stains or tags of biomolecules and in particular of antibodies. KW - ICP-MS KW - Life sciences KW - Mass cytometry KW - Laser ablation (LA)-ICP-MS KW - Bioimaging PY - 2016 DO - https://doi.org/10.1515/psr-2016-0064 SN - 2365-659X SN - 2365-6581 VL - 1 IS - 11 SP - 1 EP - 19 AN - OPUS4-40234 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Scharek, Vera M. A1 - Pfeifer, Jens A1 - Vogl, Jochen A1 - Traub, Heike A1 - Meermann, Björn T1 - Tin fractionation analysis in sediment samples via on-line ID ETV/ICP-MS N2 - We report a quantification approach for directly determining total tin and tin-based pollutants/species in sediments via electrothermal vaporization/inductively coupled plasma-mass spectrometry (ETV/ICP-MS) utilizing an on-line isotope dilution mass spectrometry (IDMS) approach. The method was developed and validated using an estuarine sediment reference material (BCR-277R), yielding a recovery of 106%. A relative standard deviation (RSD) of 14%, comparable to published data using a similar method, was obtained. A limit of quantification (LOQ) was estimated at 0.008 mg Sn kg−1 and sufficient for quantifying the total tin mass fraction of surface sediments along the tidal River Elbe course. Hereby, a decrease towards the river mouth, presumably due to dilution effects by less polluted marine sediment, was observed. Besides total tin, monitoring of organotin compounds (OTCs)/species is of interest in sediments due to their toxic effects on aquatic life. The method’s capability was extended by separating an OTC fraction in a sediment certified reference material (CRM) through the ETV temperature program. While spiking experiments with OTC standards confirmed the assignment, only a small fraction of the total certified OTC amount (3%), likely due to matrix effects, was recovered. However, applying the method to real-world samples, OTCs were detectable along the River Elbe course. By this, we demonstrated the potential of our method as a complementary fast-screening approach to species-specific analysis procedures. KW - ETV KW - Sn-Speciation KW - Sediment PY - 2025 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-640137 DO - https://doi.org/10.1007/s00216-025-06064-y SN - 1618-2642 SP - 1 EP - 10 PB - Springer Science and Business Media LLC AN - OPUS4-64013 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Drescher, D. A1 - Büchner, T. A1 - Schrade, P. A1 - Traub, Heike A1 - Werner, S. A1 - Guttmann, P. A1 - Bachmann, S. A1 - Kneipp, J. T1 - Influence of Nuclear Localization Sequences on the Intracellular Fate of Gold Nanoparticles N2 - Directing nanoparticles to the nucleus by attachment of nuclear localization sequences (NLS) is an aim in many applications. Gold nanoparticles modified with two different NLS were studied while crossing barriers of intact cells, including uptake, endosomal escape, and nuclear translocation. By imaging of the nanoparticles and by characterization of their molecular interactions with surface-enhanced Raman scattering (SERS), it is shown that nuclear translocation strongly depends on the particular incubation conditions. After an 1 h of incubation followed by a 24 h chase time, 14 nm gold particles carrying an adenoviral NLS are localized in endosomes, in the cytoplasm, and in the nucleus of fibroblast cells. In contrast, the cells display no nanoparticles in the cytoplasm or nucleus when continuously incubated with the nanoparticles for 24 h. The ultrastructural and spectroscopic data indicate different processing of NLS-functionalized particles in endosomes compared to unmodified particles. NLS functionalized nanoparticles form larger intraendosomal aggregates than unmodified gold nanoparticles. SERS spectra of cells with NLS-functionalized gold nanoparticles contain bands assigned to DNA and were clearly different from those with unmodified gold nanoparticles. The different processing in the presence of an NLS is influenced by a continuous exposure of the cells to nanoparticles and an ongoing nanoparticle uptake. This is supported by mass-spectrometry-based quantification that indicates enhanced uptake of NLS-functionalized nanoparticles compared to unmodified particles under the same conditions. The results contribute to the optimization of nanoparticle analysis in cells in a variety of applications, e.g., in theranostics, biotechnology, and bioanalytics. KW - Nanoparticle KW - Laser ablation KW - SERS KW - ICP-MS PY - 2021 DO - https://doi.org/10.1021/acsnano.1c04925 SN - 1936-086X VL - 15 IS - 9 SP - 14838 EP - 14849 PB - American Chemical Society AN - OPUS4-54047 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Erste praktische Erfahrungen mit einem ETV ICP OES-System T2 - Anwenderseminar über die direkte Analyse von Festproben mit simultaner ICP-OES / Analyse organischer und anorganischer Proben mit elektrothermischer Verdampfung (ETV) und Lichtbogen-Emission CY - Neu-Isenburg, Deutschland DA - 2002-09-23 PY - 2002 AN - OPUS4-1932 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Traub, Heike A1 - Scharf, Holger T1 - NH4NO3 extractable trace element contents of soil samples prepared for proficiency testing - a stability study N2 - In view of its intended use as a sample for proficiency testing or as a reference material the stability of the extractable trace element contents of a soil from an irrigation field was tested using the extraction with 1 mol/L ammonium nitrate solution according to DIN 19730. Therefore, changes of the extractability of sterilized and non sterilized soil samples stored at different temperatures were evaluated over a period of 18 months. Sets of bottles were kept at -20 °C, +4 °C, about +20 °C and +40 °C, respectively. The NH4NO3 extractable contents of Cd, Cr, Cu, Ni, Pb and Zn were determined immediately after bottling and then after 3, 6, 12 and 18 months with ICP-AES or ETAAS. Appropriate storage conditions are of utmost importance to prevent deterioration of soil samples prepared for the determination of NH4NO3 extractable trace element contents. Temperatures above +20 °C must be avoided. The observed changes in the extractability of the metals (especially for Cr and Cu) most likely could be related to thermal degradation of the organic matter of the soil. There is no need to sterilize dry soil samples, because microbiological activity in soils with a low moisture content appears to be negligible with regard to trace element mobilization. KW - Spurenelemente KW - Boden KW - Stabilität KW - Referenzmaterial PY - 2001 DO - https://doi.org/10.1007/s002160100748 SN - 0937-0633 VL - 370 IS - 2-3 SP - 270 EP - 274 PB - Springer CY - Berlin AN - OPUS4-1073 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Erste praktische Erfahrungen mit einem ETV-ICP-OES - System T2 - Anwenderseminar der Fa. Thermo Elemental CY - Neu-Isenburg, Germany DA - 2002-09-23 PY - 2002 AN - OPUS4-2945 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Analyse von Hochtechnologiematerialien mit ETV und Bogen T2 - Solid-Samping-User-Meeting CY - Kempten, Germany DA - 2004-05-24 PY - 2004 AN - OPUS4-3571 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Matschat, Ralf A1 - Czerwensky, Michael A1 - Traub, Heike A1 - Heinrich, Hans-Joachim A1 - Kipphardt, Heinrich T1 - Special new techniques of atomic spectrometric methods for trace analysis of high purity metals PY - 2003 SN - 0032-6895 SN - 1778-3771 VL - 91 IS - Décembre SP - 37 EP - 50, N° Hors Série CY - Paris AN - OPUS4-6188 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Direkte Feststoffanalyse von Hochtechnologie-Materialien mit ETV ICP OES und DC-Arc-OES T2 - Abteilungsseminar der Abt. I CY - Berlin, Germany DA - 2005-06-20 PY - 2005 AN - OPUS4-11909 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Comparison of different calibration strategies for the analysis of pure copper by LA-ICP-MS T2 - 13th International Conference on ultra high-purity base metals, BAM CY - Berlin, Germany DA - 2006-09-11 PY - 2006 AN - OPUS4-13480 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Determination of trace impurities in pure copper using LA-ICP-MS T2 - 16. Jahreskolloquium der Interessengemeinschaft Atomspektroskopie (IGAS) CY - Berlin, Germany DA - 2006-11-08 PY - 2006 AN - OPUS4-13778 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Analyse von reinem Kupfer mit LA-ICP-MS: Möglichkeiten der Kalibrierung mit synthetischen Standards T2 - CANAS '07, Colloquium Analytische Atomspektroskopie CY - Konstanz, Germany DA - 2007-03-18 PY - 2007 AN - OPUS4-14627 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Vergleich unterschiedlicher Ansätze zur Kalibrierung bei der Analyse von reinem Kupfer mit LA-ICP-MS T2 - ANAKON 2007 CY - Jena, Germany DA - 2007-03-27 PY - 2007 AN - OPUS4-14642 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Homogenitätsprüfung und Analyse von reinem Kupfer mit Laserablation-ICP-Massenspektrometrie T2 - 4. Hochschul-Kupfersymposium CY - Berlin, Germany DA - 2007-11-06 PY - 2007 AN - OPUS4-14803 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Traub, Heike A1 - Czerwensky, Michael A1 - Matschat, Ralf A1 - Kipphardt, Heinrich A1 - Panne, Ulrich T1 - Homogenitätsprüfung und Analyse von reinem Kupfer mit Laserablation-ICP-Massenspektrometrie KW - Laserablation KW - Kupfer KW - Homogenität KW - ZRM PY - 2007 SN - 0026-0746 VL - 61 IS - 11 SP - 744 EP - 745 PB - Giesel Verlag GmbH CY - Isernhagen AN - OPUS4-16153 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Laser ablation ICP-MS for the determination of trace impurities in pure copper T2 - EuroAnalysis XIV CY - Antwerp, Belgium DA - 2007-09-09 PY - 2007 AN - OPUS4-15692 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Application of synthetic calibration samples for the analysis of pure copper using femtosecond LA-ICP-MS T2 - 9th European Workshop on laser ablation in elemental and isotopic analysis CY - Prague, Czech Republic DA - 2008-07-07 PY - 2008 AN - OPUS4-17541 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Einsatz synthetischer Kalibrierproben für die Analyse von Reinkupfer mit fs-LA-ICP-MS T2 - 21. ICP-MS Anwendertreffen und 8. Symposium über Massenspektrometrische Verfahren der Elementspurenanalyse CY - Dresden, Germany DA - 2008-09-17 PY - 2008 AN - OPUS4-17719 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Beitrag der LA-ICP-MS zur Charakterisierung von Kupfer-Referenzmaterialien T2 - 21. ICP-MS Anwendertreffen und 8. Symposium über Massenspektrometrische Verfahren der Elementspurenanalyse CY - Dresden, Germany DA - 2008-09-17 PY - 2008 AN - OPUS4-17715 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Fast determination of the boron isotope ratio in steel and aluminium alloys using LA-ICP-MS T2 - 9th European Workshop on Laser Ablation in Elemental and Isotopic Analysis CY - Prague, Czech Republic DA - 2008-07-07 PY - 2008 AN - OPUS4-18063 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Höpken, C. A1 - Swart, Claudia A1 - Traub, Heike T1 - A fragment of a dichroic cage cup from Dülük Baba Tepesi/Doliche, Turkey KW - Diatretglas KW - LA-ICP-MS PY - 2008 SN - 0075-4250 VL - 50 SP - 303 EP - 306 CY - Corning, NY AN - OPUS4-18442 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Application of different calibration strategies for the analysis of pure copper using nano- and femtosecond laser ablation ICP-MS T2 - European Winter Conference on Plasma Spectrochemistry CY - Graz, Austria DA - 2009-02-15 PY - 2009 AN - OPUS4-19007 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Wienold, Julia A1 - Traub, Heike A1 - Lange, Britta A1 - Giray, Thorsten A1 - Recknagel, Sebastian A1 - Kipphardt, Heinrich A1 - Matschat, Ralf A1 - Panne, Ulrich T1 - Elemental analysis of copper and magnesium alloy samples using IR-laser ablation in comparison with spark and glow discharge methods N2 - Three methods for direct solid sampling of bulk material namely IR laser ablation, glow discharge and spark OES, were compared with respect to analytical figures of merit obtained for elemental analysis with atomic spectrometry. Matrices investigated were copper, pressed doped copper powder, and magnesium alloys. For the vast majority of analytes, statistical equivalence regarding precision (usually ≤ 5%) and the performance of the calibrations between the compared methods was demonstrated. KW - LA-ICP-MS KW - GD-MS KW - Spark-OES KW - LA-ICP-OES KW - Copper KW - Magnesium PY - 2009 DO - https://doi.org/10.1039/b903251g SN - 0267-9477 SN - 1364-5544 VL - 24 SP - 1570 EP - 1574 PB - Royal Society of Chemistry CY - London AN - OPUS4-20257 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Müller, Larissa A1 - Traub, Heike A1 - Jakubowski, Norbert A1 - Drescher, Daniela A1 - Baranov, Vladimir A1 - Kneipp, Janina T1 - Trends in single-cell analysis by use of ICP-MS N2 - The analysis of single cells is a growing research field in many disciplines such as toxicology, medical diagnosis, drug and cancer research or metallomics, and different methods based on microscopic, mass spectrometric, and spectroscopic techniques are under investigation. This review focuses on the most recent trends in which inductively coupled plasma mass spectrometry (ICP-MS) and ICP optical emission spectrometry (ICP-OES) are applied for single-cell analysis using metal atoms being intrinsically present in cells, taken up by cells (e.g., nanoparticles), or which are artificially bound to a cell. For the latter, especially element tagged antibodies are of high interest and are discussed in the review. The application of different sample introduction systems for liquid analysis (pneumatic nebulization, droplet generation) and elemental imaging by laser ablation ICP-MS (LA-ICP-MS) of single cells are highlighted. Because of the high complexity of biological systems and for a better understanding of processes and dynamics of biologically or medically relevant cells, the authors discuss the idea of 'multimodal spectroscopies.' KW - Bioanalytical methods KW - Cell systems/single cell analysis KW - Mass spectrometry/ICP-MS PY - 2014 DO - https://doi.org/10.1007/s00216-014-8143-7 SN - 1618-2642 SN - 1618-2650 VL - 406 IS - 27 SP - 6963 EP - 6977 PB - Springer CY - Berlin AN - OPUS4-31717 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Büchner, Tina A1 - Drescher, Daniela A1 - Traub, Heike A1 - Schrade, P. A1 - Bachmann, S. A1 - Jakubowski, Norbert A1 - Kneipp, Janina T1 - Relating surface-enhanced Raman scattering signals of cells to gold nanoparticle aggregation as determined by LA-ICP-MS micromapping N2 - The cellular response to nanoparticle exposure is essential in various contexts, especially in nanotoxicity and nanomedicine. Here, 14-nm gold nanoparticles in 3T3 fibroblast cells are investigated in a series of pulse-chase experiments with a 30-min incubation pulse and chase times ranging from 15 min to 48 h. The gold nanoparticles and their aggregates are quantified inside the cellular ultrastructure by laser ablation inductively coupled plasma mass spectrometry micromapping and evaluated regarding the surface-enhanced Raman scattering (SERS) signals. In this way, both information about their localization at the micrometre scale and their molecular nanoenvironment, respectively, is obtained and can be related. Thus, the nanoparticle pathway from endocytotic uptake, intracellular processing, to cell division can be followed. It is shown that the ability of the intracellular nanoparticles and their accumulations and aggregates to support high SERS signals is neither directly related to nanoparticle amount nor to high local nanoparticle densities. The SERS data indicate that aggregate geometry and interparticle distances in the cell must change in the course of endosomal maturation and play a critical role for a specific gold nanoparticle type in order to act as efficient SERS nanoprobe. This finding is supported by TEM images, showing only a minor portion of aggregates that present small interparticle spacing. The SERS spectra obtained after different chase times show a changing composition and/or structure of the biomolecule corona of the gold nanoparticles as a consequence of endosomal processing. KW - Gold nanoparticles KW - Surface-enhanced Raman scattering KW - LA-ICP-MS KW - Fibroblast KW - Cell KW - Particle aggregation KW - Endosome PY - 2014 DO - https://doi.org/10.1007/s00216-014-8069-0 SN - 1618-2642 SN - 1618-2650 VL - 406 IS - 27 SP - 7003 EP - 7014 PB - Springer CY - Berlin AN - OPUS4-31718 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Studying cellular uptake and processing of nanoparticles by LA-ICP-MS N2 - In recent years, elemental imaging of biological samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is gaining in importance. Recent improvements regarding spatial resolution (down to 1 µm) and washout time make LA-ICP-MS particularly interesting for single cell analysis. Many current nanomaterials can serve as contrast agents in cellular or tissue imaging, drug delivery vehicles or therapeutics, whereas others can cause toxic effects. In order to evaluate nano-bio interactions, the number of nanoparticles (NPs) inside cells as well as their localisation within cellular substructures is of particular interest. LA-ICP-MS was used to study the NP pathway from uptake, via intracellular processing up to cell division. Fibroblast cells were incubated with different metallic NPs under varying experimental conditions. For LA analysis the cells were fixed with formaldehyde and dried. Our results show that LA-ICP-MS is able to localise NP aggregates within cellular substructures. The NPs accumulate in the perinuclear region in the course of intracellular processing, e.g. multivesicular fusion and endosomal maturation, but do not enter the nucleus [1, 2]. A strong dependence of NP uptake on concentration and incubation time was found. Additionally, the number of NPs internalized by individual cells was determined and variations within the cell population became visible. A new laser ablation system providing a short washout time (50 ms) together with small spot sizes (< 4 µm) and high repetition rates allows high spatial resolution applications. First results of cell imaging will be shown. The findings demonstrate the potential of LA-ICP-MS providing insight into NP uptake and intracellular distribution dependent on experimental parameters. T2 - 13th European Workshop on Laser Ablation CY - Ljubljana, Slovenia DA - 12.07.2016 KW - LA-ICP-MS KW - Nanoparticle KW - Bio-imaging KW - Cell PY - 2016 AN - OPUS4-36891 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Studying nanoparticle-cell interaction by LA ICP-MS N2 - The interaction of nanoparticles (NPs) with cells has become a major field of interest, ranging from medical applications to nanotoxicology. Size, shape and surface modification of the NPs determine the uptake rate and pathway into the cells, and therefore impact specific cell components and processes. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is an established quantitative multi-elemental analysis and mapping technique. In recent years, it was shown that LA-ICP-MS can provide quantitative as well as distribution information of NPs in cell samples. Here LA-ICP-MS was applied for the imaging of individual cells to study the uptake and intracellular processing of metal-containing nanostructures. Additionally, the local distribution of naturally occurring elements in cells like P was measured to indicate cell morphology. The cells were incubated with different types of NPs under varying experimental conditions. For LA analysis, the cells were fixed and dried. Our findings show, that LA-ICP-MS is suitable for the localisation of nanoparticle aggregates within cellular compartments. The studied NPs accumulate in the perinuclear region in the course of intracellular processing, but do not enter the cell nucleus. The uptake efficiency depends strongly on the physicochemical properties of the nanostructures as well as on the incubation conditions like concentration and incubation time. The results demonstrate the potential of LA-ICP-MS providing insight into NP uptake, intracellular distribution and cell-to-cell variation dependent on experimental parameters. T2 - Workshop on Laser Bioimaging Mass Spectrometry CY - Münster, Germany DA - 24.05.2018 KW - Imaging KW - Laser ablation KW - ICP-MS KW - Nanoparticle KW - Cell PY - 2018 AN - OPUS4-45071 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - LA-ICP-MS to study nanoparticle-cell interaction N2 - Nanoparticles (NPs) have found a wide range of applications in research and industry. Thereby the interaction of NPs with biological systems like cells has become a major field of interest, ranging from medical applications to nanotoxicology. Size, shape and surface modification of the nanomaterials determine the uptake rate and pathway into the cells, and therefore impact specific cell components and processes. In recent years, elemental imaging of biological samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is gaining more and more importance. Improvements concerning both spatial resolution (down to 1 µm) and signal-to-background ratio due to low-dispersion LA chambers make LA-ICP-MS particularly interesting for single cell analysis. Here LA-ICP-MS was applied for the imaging of individual cells to study the uptake and intracellular processing of metal-containing nanostructures. The cells were incubated with different NPs under varying experimental conditions and afterwards fixed with para-formaldehyde and dried for LA analysis. High-spatial resolution LA-ICP-MS was achieved by careful optimisation of the laser ablation parameters. Our findings show, that LA-ICP-MS is applicable to localize NP aggregates within cellular compartments. The uptake efficiency depends strongly on the physicochemical properties of the nanostructures as well as on the incubation conditions like concentration and incubation time. The results demonstrate the potential of LA-ICP-MS providing insight into nanoparticle-cell interaction dependent on experimental parameters. T2 - 14th European Workshop on Laser Ablation (EWLA) CY - Pau, France DA - 26.06.2018 KW - Laser ablation KW - ICP-MS KW - Imaging KW - Nanoparticle KW - Cell PY - 2018 AN - OPUS4-45570 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike A1 - Phukphatthanachai, Pranee T1 - Quantification of sulphur in copper metals by isotope dilution LA-ICP-MS using polyethylene frits N2 - Sulphur is one of the relevant impurities in copper and its alloys affecting their material properties. To ensure the quality of copper products, fast direct solid sampling techniques are very attractive. However, for the calibration suitable matrix reference materials are required. For the certification of such reference materials appropriate, SI-traceable analytical methods are essential. Therefore, a procedure was developed to quantify total sulphur in copper by combining the classical isotope dilution (ID) technique and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Here, for the first time, polyethylene (PE) frits were used to prepare appropriate solid samples for the sulphur quantification in copper metals (alloyed/unalloyed) by isotope dilution LA-ICP-MS. The properties of the PE frit meet the requirements as porous material with high absorption efficiency, thermal and chemical resistance as well as low sulphur blank. Different copper reference materials were used to develop and validate the procedure. The copper samples were spiked with 34S, digested with nitric acid and then the digests were absorbed on PE frits. After drying, the frits were analysed by LA-ICP-IDMS using a Nd:YAG laser at 213 nm coupled to an ICP sector field mass spectrometer. It could be demonstrated, that the sample solution dispersed on the frits did not influence the 32S/34S ratio significantly even though the sulphur intensities were fluctuating along the scanned lines. Relative standard deviations of the isotope ratios were below 5 % in average between three line scans (except for the pure spike solution and procedure blank). The measurement results were validated by comparing them with the results obtained by conventional ICP-IDMS after analyte-matrix separation. Plotting the mass fraction of sulphur in copper obtained by LA-ICP-IDMS versus those obtained by ICP-IDMS yields a linear curve with a correlation coefficient of 0.9999 showing a strong agreement between both techniques. The metrological traceability to the SI from the kg down to the sulphur mass fraction in copper is established by an unbroken chain of comparisons, each accompanied by an uncertainty budget. Thus, the measurement results are considered reliable, acceptable and comparable within the stated measurement uncertainty. T2 - 14th European Workshop on Laser Ablation (EWLA) CY - Pau, France DA - 26.06.2018 KW - ICP-MS KW - Laser ablation KW - Isotope dilution KW - Copper PY - 2018 AN - OPUS4-45569 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Drescher, D. A1 - Traub, Heike A1 - Büchner, T. A1 - Jakubowski, Norbert A1 - Kneipp, J. T1 - Properties of in situ generated gold nanoparticles in the cellular context N2 - Gold nanostructures that serve as probes for nanospectroscopic analysis of eukaryotic cell cultures can be obtained by the in situ reduction of tetrachloroauric acid (HAuCl4). To understand the formation process of such intracellularly grown particles depending on the incubation medium, the reaction was carried out with 3T3 fibroblast cells in three different incubation media, phosphate buffer, Dulbecco's Modified Eagle Medium (DMEM), and standard cell culture medium (DMEM with fetal calf serum). The size, the optical properties, the biomolecular corona, and the localization of the gold nanoparticles formed in situ vary for the different conditions. The combination of surface-enhanced Raman scattering (SERS) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) microscopic mapping and transmission electron microscopy (TEM) provides complementary perspectives on plasmonic nanoparticles and non-plasmonic gold compounds inside the cells. While for the incubation with HAuCl4 in PBS, gold particles provide optical signals from the nucleus, the incubation in standard cell culture medium leads to scavenging of the toxic molecules and the formation of spots of high gold concentration in the cytoplasm without formation of SERS-active particles inside the cells. The biomolecular corona of nanoparticles formed in situ after incubation in buffer and DMEM differs, suggesting that different intracellular molecular species serve for reduction and stabilization. Comparison with data obtained from ready-made gold nanoparticles suggests complementary application of in situ and ex situ generated nanostructures for optical probing. KW - Nanoparticles KW - Laser ablation KW - ICP-MS KW - SERS KW - Cell PY - 2017 DO - https://doi.org/10.1039/C7NR04620K SN - 2040-3372 VL - 9 IS - 32 SP - 11647 EP - 11656 PB - The Royal Society of Chemistry RSC CY - Cambridge, UK AN - OPUS4-41871 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Studying cellular uptake of metal-containing nanoparticles by LA-ICP-MS N2 - Nanoparticles (NPs) have potential applications in medical diagnostics, imaging, drug delivery and other kinds of therapy. Furthermore, studies concerning nanoparticle uptake by cells are important for risk assessment. Size, shape and surface modification of the NPs determine the uptake rate and pathway into the cells, and therefore impact specific cell components and processes. Understanding the different uptake mechanisms and involved processes require sub-cellular resolution to determine, for example, whether the nanoparticles are reaching the nucleus. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is an established quantitative multi-elemental analysis and mapping technique. However, sub-cellular imaging has traditionally been challenging to achieve due to a lack of sensitivity at small laser spots. But now novel laser ablation systems with improved sensitivity and washout time allow imaging at high lateral resolution with spot sizes down to 1 µm. Here LA-ICP-MS was applied for the imaging of individual fibroblast cells to study the uptake and intracellular processing of metal-containing NPs. To indicate cell morphology the local distribution of naturally occurring elements in cells like P and Zn was measured, too. Our results show that LA-ICP-MS can be used to localise nanoparticle aggregates within cellular compartments. The studied NPs accumulate in the perinuclear region in the course of intracellular processing, but do not enter the cell nucleus. The uptake efficiency depends strongly on the physico-chemical properties of the nanostructures as well as on the incubation conditions like concentration and incubation time. The potential of LA-ICP-MS for analysis at single cell level will be demonstrated. T2 - Euroanalysis 2017 CY - Stockholm, Sweden DA - 28.08.2017 KW - Laser ablation KW - ICP-MS KW - Nanoparticle KW - Cell PY - 2017 AN - OPUS4-41883 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Jakubowski, Norbert A1 - Müller, Larissa A1 - Traub, Heike A1 - Esteban-Fernandez, Diego A1 - Panne, Ulrich A1 - Herrmann, Antje A1 - Schellenberger, E. A1 - Kneipp, Janina T1 - Lecture 5 Bio- and immuno-imaging by use of laser ablation ICP-MS N2 - ICP-MS is a well-established analytical method which excels by high accuracy, high dynamic range and extremely low limits of detection for most metals. Furthermore ICP-MS offers a very high multi-element coverage so that many elements of the periodic table can be detected simultaneously. In this series of lectures, we want to focus on the historical developments, fundamentals, instrumentation and novel applications of ICP-MS in the life and material sciences. KW - Imaging KW - Laser ablation ICP-MS PY - 2017 AN - OPUS4-40943 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CHAP A1 - Müller, L. A1 - Traub, Heike A1 - Jakubowski, Norbert ED - Prof. Dr. Golloch, Alfred T1 - Novel applications of lanthanoides as analytical or diagnostic tools in the life sciences by ICP-MS-based techniques N2 - Inductively coupled plasma mass spectrometry (ICP-MS) is a well-established analytical method for multi-elemental analysis in particular for elements at trace and ultra-trace levels. It has found acceptance in various application areas during the last decade. ICP-MS is also more and more applied for detection in the life sciences. For these applications, ICP-MS excels by a high sensitivity, which is independent of the molecular structure of the analyte, a wide linear dynamic range and by excellent multi-element capabilities. Furthermore, methods based on ICP-MS offer simple quantification concepts, for which usually (liquid) standards are applied, low matrix effects compared to other conventional bioanalytical techniques, and relative limits of detection (LODs) in the low pg g−1 range and absolute LODs down to the attomol range. In this chapter, we focus on new applications where the multi-element capability of ICP-MS is used for detection of lanthanoides or rare earth elements, which are applied as elemental stains or tags of biomolecules and in particular of antibodies. KW - ICP-MS KW - Life sciences KW - Mass cytometry KW - Laser ablation (LA)-ICP-MS KW - Bioimaging PY - 2017 SN - 978-3-11-036523-8 SP - Chapter 11, 301 EP - 320 PB - De Gruyter AN - OPUS4-40244 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schannor, Mathias A1 - Oelze, Marcus A1 - Traub, Heike A1 - He, Yubei A1 - Schmidt, Robin A1 - Heidemann, Luisa A1 - Savic, Lynn Jeanette A1 - Vogl, Jochen A1 - Meermann, Björn T1 - Advancing Biomarker Research: In Situ Cu Isotope Analysis in Liver Tumors by LA-MC-ICP-MS N2 - Stable metal isotopes have received increasing attention as medical biomarkers due to their potential to detect changes in metal metabolism related to diseases. In particular, copper stable isotopes are a powerful tool to identify isotopic variation between tumors and healthy tissue, suggesting application in cancer diagnosis. However, potential mechanisms causing isotope fractionation, such as redox- or bond-forming reactions and interactions of metals during transmembrane import and export, are less well understood. Here, we established an in situ method using laser ablation-multicollector-inductively coupled plasma-mass spectrometry (LA-MC-ICP-MS) to advance our understanding of the underlying processes responsible for isotope fractionation between normal and diseased tissues. Gelatin-based bracketing standards and quality control reference materials, crucial for laser ablation analysis, were developed to allow correction for instrumentally induced isotope fractionation during LA-MC-ICP-MS analysis. Using such matrix-matched standards, the method achieved intermediate precisions for delta values of better than 0.15 ‰ (2 s) for inorganic reference materials and of better than 0.17 ‰ (2 s) for biological reference materials. The developed routine was tested on rabbit VX2 liver tumor samples, a model system resembling human hepatocellular carcinoma (HCC) used to study liver cancer. In situ Cu isotope compositions between healthy (𝛿65/63NIST976(Cu) = −1.5 ‰ to 0.2 ‰) and tumorous (𝛿65/63NIST976(Cu) = 0.0 ‰ to 1.3 ‰) liver tissue show distinct differences in their isotope ratios. The observed isotopic dichotomy is consistent with previous solution-based MC-ICP-MS work, showing enrichment of heavy 65Cu in cancer biopsies relative to healthy tissue. KW - Biomarker KW - Laser Ablation KW - Reference Material PY - 2025 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-626865 DO - https://doi.org/10.1021/acs.analchem.4c05626 SN - 1520-6882 VL - 97 IS - 8 SP - 4425 EP - 4432 PB - American Chemical Society (ACS) AN - OPUS4-62686 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Löwa, N. A1 - Golusda, L. A1 - Paclik, D. A1 - Traub, Heike A1 - Schannor, Mathias A1 - Saatz, Jessica A1 - Freise, C. A1 - Taupitz, M. A1 - Siegmund, B. A1 - Kühl, A. A. A1 - Wiekhorst, F. T1 - Magnetic particle spectroscopy for Eu-VSOP quantification in intestinal inflammation: Distinguishing nanoparticle signals from dietary contamination N2 - Magnetic nanoparticles are gaining increasing attention as a promising alternative to gadolinium-based contrast agents in magnetic resonance imaging, primarily due to their low toxicity. In this study, we investigated the use of magnetic iron oxide nanoparticles in mouse models of intestinal inflammation to assess their potential for detecting changes in the extracellular matrix. For magnetic quantification, we employed magnetic particle spectroscopy, which offers high sensitivity and minimal interference from biological tissue. However, we observed significant variations in magnetic signals within the intestine, as well as measurable signals in control animals, indicating possible magnetic contamination. By doping the nanoparticles with europium, we were able to confirm this suspicion through quantitative elemental analysis. Examination of mouse feed and feces allowed us to identify the source of contamination. Based on these findings, we developed a method to reliably distinguish genuine signals of magnetic nanoparticles from those caused by external magnetic contaminations. This approach is essential to ensure reliable results in future diagnostic and preclinical research. KW - ICP-MS KW - Nanoparticle KW - Magnetic particle spectroscopy KW - Quantification PY - 2025 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-642238 DO - https://doi.org/10.1039/D5NA00452G SN - 2516-0230 IS - 00452 SP - 1 EP - 10 PB - Royal Society of Chemistry (RSC) CY - Cambridge AN - OPUS4-64223 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Phukphatthanachai, Pranee A1 - Vogl, Jochen A1 - Traub, Heike A1 - Jakubowski, Norbert A1 - Panne, Ulrich T1 - A new approach of using polyethylene frits for the quantification of sulphur in copper metals by isotope dilution LA-ICP-MS and comparison with conventional IDMS techniques N2 - Polyethylene (PE) frits were used to quantify sulphur in copper and its alloys by isotope dilution combined with LA-ICP-MS as an alternative approach to conventional sample preparation: the copper samples were spiked, the spiked samples were dissolved, the resulting solutions were absorbed in the PE frits and finally the PE frits were analysed by LA-ICP-MS. A prerequisite for such a support material is a low sulphur blank and thus PE was selected for this purpose. The absorption efficiency of the PE frits was studied for varying sulphur amounts ranging from 2 mg S to 80 mg S showing that more than 99.5% of the loaded sulphur was absorbed by the frit. The so prepared PE frits were measured by LA-ICP-MS and yielded a good linearity (R2 ¼ 0.999) for the sulphur ion intensities corresponding to sulphur amounts up to 40 mg S; the associated sensitivity is approximately 3.4 x 10⁴ cps μg⁻¹ for ³²S. For the validation of the developed procedure the reference materials BAM-M376a, BAM-228 and BAM-227 were applied such that 2 μg S, 5 μg S and 11 μg S were absorbed in the PE frits, respectively. These samples were pre-quantified for the adsorbed sulphur amount by external calibration LA-ICP-MS yielding sulphur amounts of 0.9 μg, 5.1 μg and 8.5 μg (quantified for ³²S only), respectively. Relative Standard deviations of the isotope ratios were below 5% in average (n ¼ 3 lines) in all cases (except for the pure spike solution). These samples were then analysed by LA-ICP-IDMS and the measurement results were validated by comparing them with the results obtained by conventional ICP-IDMS. The obtained relative expanded measurement uncertainties ranged between 10% and 26%. Pearson's coefficient was used to express the correlation between both techniques; the obtained value was 0.999 demonstrating a strong correlation. Contrary to most published LA-ICP-IDMS procedures, the developed procedure enables SI-traceability for the measurement results. The metrological traceability to the SI for the sulphur mass fractions in copper was established by an unbroken chain of comparisons, each accompanied by an uncertainty budget. Thus, the measurement results are considered reliable, acceptable and comparable within the stated measurement uncertainty. The metrological traceability chain from the kg down to mass fraction in the samples obtained by LA-ICP-IDMS is presented as well. KW - Laser ablation KW - IDMS KW - Traceability KW - Uncertainty KW - Purity PY - 2018 DO - https://doi.org/10.1039/c8ja00116b SN - 0267-9477 SN - 1364-5544 VL - 33 IS - 9 SP - 1506 EP - 1517 PB - Royal Society of Chemistry CY - Cambridge AN - OPUS4-45899 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - Studying nanoparticle-cell interaction by ICP-MS based techniques N2 - Nanoparticles (NPs) have found a wide range of applications in research and industry. Thereby the interaction of NPs with biological systems like cells has become a major field of interest, ranging from medical applications to nanotoxicology. Size, shape and surface modification of the nanomaterials determine the uptake rate and pathway into the cells, and therefore impact specific cell components and processes. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is an established quantitative multi-elemental analysis and mapping technique. In recent years, it was shown that LA-ICP-MS can provide quantitative as well as distribution information of metal containing nanoparticles (NPs) in cell samples. Here LA-ICP-MS was applied for the imaging of individual fibroblast cells to study the uptake and intracellular processing of NPs. Our results show that LA-ICP-MS can be used to localize nanoparticle aggregates within cellular compartments. The studied NPs accumulate in the perinuclear region in the course of intracellular processing, but do not enter the cell nucleus. The uptake efficiency depends strongly on the physico-chemical properties of the nanostructures as well as on the incubation conditions like concentration and incubation time. ICP-MS was used to determine the composition of the nanomaterials as well as the number of NPs in cells after acid digestion of the samples. T2 - 13. Symposium „Massenspektrometrische Verfahren der Element­spurenanalyse“ & 26. ICP-MS-Anwendertreffen CY - Berlin, Germany DA - 03.09.2018 KW - ICP-MS KW - Nanoparticle KW - Cell KW - Laser ablation PY - 2018 AN - OPUS4-45860 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike T1 - LA-ICP-MS for the analysis of nanoparticles in cells N2 - Many current nanomaterials can serve as contrast agents in cellular or tissue imaging, drug delivery vehicles or therapeutics, whereas others can cause toxic effects. In order to evaluate nano-bio interactions, the number of nanoparticles (NPs) inside cells as well as their localisation within cellular substructures is of particular interest. The cellular uptake depends on the primary characteristics of the NPs (e.g. size, shape, surface coating) and on the cell type. Laser ablation inductively coupled plasma mass spectrometry (LA‑ICP‑MS) is more and more used to study the NP pathway from uptake, via intracellular processing up to cell division. High-spatial resolution laser ablation at single cell level is achieved using novel low-dispersion LA chambers and by careful optimisation of laser energy, ablation frequency and scan speed at small laser spot sizes down to 1 µm. Different examples from BAM, Division 1.1 and cooperation partners using LA-ICP-MS to localize and quantify metal-containing nanoparticles are shown. The results demonstrate the potential of LA-ICP-MS providing insight into NP uptake, intracellular distribution and cell-to-cell variation. T2 - 13. Symposium "Massensprektrometrische Verfahren der Elementspurenanalyse" + 26. ICP-MS Anwendertreffen CY - Berlin, Germany DA - 03.09.2018 KW - Laser ablation KW - Cells KW - Nanoparticles KW - Imaging PY - 2018 AN - OPUS4-46440 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Drescher, Daniela A1 - Giesen, Charlotte A1 - Traub, Heike A1 - Panne, Ulrich A1 - Kneipp, Janina A1 - Jakubowski, Norbert T1 - Quantitative imaging of gold and silver nanoparticles in single eukaryotic cells by laser ablation ICP-MS N2 - Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was utilized for spatially resolved bioimaging of the distribution of silver and gold nanoparticles in individual fibroblast cells upon different incubation experiments. High spatial resolution was achieved by optimization of scan speed, ablation frequency, and laser energy. Nanoparticles are visualized with respect to cellular substructures and are found to accumulate in the perinuclear region with increasing incubation time. On the basis of matrix-matched calibration, we developed a method for quantification of the number of metal nanoparticles at the single-cell level. The results provide insight into nanoparticle/cell interactions and have implications for the development of analytical methods in tissue diagnostics and therapeutics. KW - Imaging KW - Cell KW - Nanoparticles KW - Laser ablation KW - ICP-MS PY - 2012 DO - https://doi.org/10.1021/ac302639c SN - 0003-2700 SN - 1520-6882 VL - 84 IS - 22 SP - 9684 EP - 9688 PB - American Chemical Society CY - Washington, DC AN - OPUS4-27440 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -