TY - CONF A1 - Esteban-Fernandez, Diego A1 - Traub, Heike A1 - Hutchinson, R. A1 - Jakubowski, Norbert A1 - Wilkins, J. A1 - Summerfield, L. A1 - McLachlin, K. T1 - High resolution laser ablation NWRimage system for single cell imaging N2 - The traceability and availability of nanoparticles enables their use to enhance a variety of nano-biological and nano-medicinal applications. The particular size and shape of nanoparticles determine the uptake rate and pathway into the cell, and therefore impact specific cell components and processes. Selecting specific particle types allows researchers to target the process or structure of interest, with minimal additional impact. This can be used for drug or DNA delivery, and is being explored for use in oncology. Understanding the different uptake mechanisms and impacted processes requires sub-cellular Imaging resolution to determine, for example, whether or not the nanoparticles are reaching the nucleus. Sub-cellular imaging has traditionally been challenging to achieve with laser ablation ICP-MS due to a lack of sensitivity at small spots. Bioimaging using LA-ICP-MS is a well-established technique, but usually applied on the tissue scale, which depends on larger spot areas where sensitivity is less problematic. The improved sensitivity and washout from the NWRimage has allowed faster imaging of smaller spots. The NWRimage also provides the possibility of true sub-micron spot sizes. This work compares the capabilities of standard laser Ablation (NWR213 system) with results from the NWRimage platform, which has been optimized for imaging applications. T2 - European Winter Conference on Plasma Spectrochemistry CY - St. Anton, Austria DA - 19.02.2017 KW - Laser ablation KW - Imaging KW - Nanoparticle PY - 2017 AN - OPUS4-39281 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike A1 - Drescher, D. A1 - Büchner, T. A1 - Kneipp, J. A1 - Jakubowski, Norbert T1 - Imaging of nanoparticles in cells by LA-ICP-MS N2 - Studying the interaction of nanoparticles (NPs) with cells has become a growing field of interest. Research topics are ranging from nanotoxicology to medical applications e.g. as theranostic agents. In order to evaluate nano-bio interactions, the number of NPs inside cells as well as their localisation within cellular substructures is of particular interest. In recent years, elemental imaging of biological samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is gaining more and more importance. Latest instrumental developments regarding spatial resolution (down to 1 µm) and detection efficiency make LA ICP-MS particularly interesting for single cell analysis. Here, we have applied LA-ICP-MS for sub-cellular scale imaging of individual cells to study the NP pathway from uptake, via intracellular processing up to cell division. Furthermore, the local distribution of naturally occurring elements in cells like P was measured to indicate the cell morphology. Murine fibroblast cells were incubated with different metal-containing NPs under varying experimental conditions. For LA analysis, the cells were fixed and dried. Sub-cellular resolution was achieved by careful optimisation of the laser ablation parameters. By rastering with the laser beam across the sample, a two-dimensional image of the elemental distribution can be received. Our results show that LA-ICP-MS is able to localise NP aggregates within cellular substructures. The studied NPs accumulate in the perinuclear region in the course of intracellular processing, e.g. multivesicular fusion and endosomal maturation, but do not enter the nucleus. The uptake depends on the physico-chemical properties of the nanostructures and on the incubation conditions like concentration and incubation time. Additionally, the number of NPs internalized by individual cells was determined and variations within a cell population became visible. The findings demonstrate the potential of LA-ICP-MS providing insight into NP uptake and intracellular distribution dependent on experimental parameters. T2 - Euroanalysis 2017 CY - Stockholm, Sweden DA - 28.08.2017 KW - Laser ablation KW - Nanoparticle KW - ICP-MS KW - Imaging KW - Cell PY - 2017 AN - OPUS4-41884 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -