TY - JOUR A1 - Drescher, Daniela A1 - Zeise, Ingrid A1 - Traub, Heike A1 - Guttmann, P. A1 - Seifert, Stephan A1 - Büchner, Tina A1 - Jakubowski, Norbert A1 - Schneider, G. A1 - Kneipp, Janina T1 - In situ characterization of SiO2 nanoparticle biointeractions using BrightSilica N2 - By adding a gold core to silica nanoparticles (BrightSilica), silica-like nanoparticles are generated that, unlike unmodified silica nanoparticles, provide three types of complementary information to investigate the silica nano-biointeraction inside eukaryotic cells in situ. Firstly, organic molecules in proximity of and penetrating into the silica shell in live cells are monitored by surface-enhanced Raman scattering (SERS). The SERS data show interaction of the hybrid silica particles with tyrosine, cysteine and phenylalanine side chains of adsorbed proteins. Composition of the biomolecular corona of BrightSilica nanoparticles differs in fibroblast and macrophage cells. Secondly, quantification of the BrightSilica nanoparticles using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) micromapping indicates a different interaction of silica nanoparticles compared to gold nanoparticles under the same experimental conditions. Thirdly, the metal cores allow the investigation of particle distribution and interaction in the cellular ultrastructure by cryo nanoscale X-ray tomography (cryo-XT). In 3D reconstructions the assumption is confirmed that BrightSilica nanoparticles enter cells by an endocytotic mechanism. The high SERS intensities are explained by the beneficial plasmonic properties due to agglomeration of BrightSilica. The results have implications for the development of multi-modal qualitative and quantitative characterization in comparative nanotoxicology and bionanotechnology. KW - Silica nanoparticles KW - Surface-enhanced Raman scattering KW - X-ray tomography KW - LA-ICP-MS KW - Core–shell structures PY - 2014 U6 - https://doi.org/10.1002/adfm.201304126 SN - 1616-301X SN - 1616-3028 VL - 24 IS - 24 SP - 3765 EP - 3775 PB - Wiley-VCH CY - Weinheim AN - OPUS4-30924 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Müller, Larissa A1 - Traub, Heike A1 - Jakubowski, Norbert A1 - Drescher, Daniela A1 - Baranov, Vladimir A1 - Kneipp, Janina T1 - Trends in single-cell analysis by use of ICP-MS N2 - The analysis of single cells is a growing research field in many disciplines such as toxicology, medical diagnosis, drug and cancer research or metallomics, and different methods based on microscopic, mass spectrometric, and spectroscopic techniques are under investigation. This review focuses on the most recent trends in which inductively coupled plasma mass spectrometry (ICP-MS) and ICP optical emission spectrometry (ICP-OES) are applied for single-cell analysis using metal atoms being intrinsically present in cells, taken up by cells (e.g., nanoparticles), or which are artificially bound to a cell. For the latter, especially element tagged antibodies are of high interest and are discussed in the review. The application of different sample introduction systems for liquid analysis (pneumatic nebulization, droplet generation) and elemental imaging by laser ablation ICP-MS (LA-ICP-MS) of single cells are highlighted. Because of the high complexity of biological systems and for a better understanding of processes and dynamics of biologically or medically relevant cells, the authors discuss the idea of 'multimodal spectroscopies.' KW - Bioanalytical methods KW - Cell systems/single cell analysis KW - Mass spectrometry/ICP-MS PY - 2014 U6 - https://doi.org/10.1007/s00216-014-8143-7 SN - 1618-2642 SN - 1618-2650 VL - 406 IS - 27 SP - 6963 EP - 6977 PB - Springer CY - Berlin AN - OPUS4-31717 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Büchner, Tina A1 - Drescher, Daniela A1 - Traub, Heike A1 - Schrade, P. A1 - Bachmann, S. A1 - Jakubowski, Norbert A1 - Kneipp, Janina T1 - Relating surface-enhanced Raman scattering signals of cells to gold nanoparticle aggregation as determined by LA-ICP-MS micromapping N2 - The cellular response to nanoparticle exposure is essential in various contexts, especially in nanotoxicity and nanomedicine. Here, 14-nm gold nanoparticles in 3T3 fibroblast cells are investigated in a series of pulse-chase experiments with a 30-min incubation pulse and chase times ranging from 15 min to 48 h. The gold nanoparticles and their aggregates are quantified inside the cellular ultrastructure by laser ablation inductively coupled plasma mass spectrometry micromapping and evaluated regarding the surface-enhanced Raman scattering (SERS) signals. In this way, both information about their localization at the micrometre scale and their molecular nanoenvironment, respectively, is obtained and can be related. Thus, the nanoparticle pathway from endocytotic uptake, intracellular processing, to cell division can be followed. It is shown that the ability of the intracellular nanoparticles and their accumulations and aggregates to support high SERS signals is neither directly related to nanoparticle amount nor to high local nanoparticle densities. The SERS data indicate that aggregate geometry and interparticle distances in the cell must change in the course of endosomal maturation and play a critical role for a specific gold nanoparticle type in order to act as efficient SERS nanoprobe. This finding is supported by TEM images, showing only a minor portion of aggregates that present small interparticle spacing. The SERS spectra obtained after different chase times show a changing composition and/or structure of the biomolecule corona of the gold nanoparticles as a consequence of endosomal processing. KW - Gold nanoparticles KW - Surface-enhanced Raman scattering KW - LA-ICP-MS KW - Fibroblast KW - Cell KW - Particle aggregation KW - Endosome PY - 2014 U6 - https://doi.org/10.1007/s00216-014-8069-0 SN - 1618-2642 SN - 1618-2650 VL - 406 IS - 27 SP - 7003 EP - 7014 PB - Springer CY - Berlin AN - OPUS4-31718 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -