TY - JOUR A1 - Chowdhary, S. A1 - Moschner, J. A1 - Mikolajczak, D. J. A1 - Becker, M. A1 - Thünemann, Andreas A1 - Kästner, Claudia A1 - Klemczak, D. A1 - Stegemann, A.-K. A1 - Böttcher, C. A1 - Metrangolo, P. A1 - Netz, R. R. A1 - Koksch, B. T1 - The Impact of Halogenated Phenylalanine Derivatives on NFGAIL Amyloid Formation JF - ChemBioChem N2 - The hexapeptide hIAPP22–27 (NFGAIL) is known as a crucial amyloid core sequence of the human islet amyloid polypeptide (hIAPP) whose aggregates can be used to better understand the wild‐type hIAPP′s toxicity to β‐cell death. In amyloid research, the role of hydrophobic and aromatic‐aromatic interactions as potential driving forces during the aggregation process is controversially discussed not only in case of NFGAIL, but also for amyloidogenic peptides in general. We have used halogenation of the aromatic residue as a strategy to modulate hydrophobic and aromatic‐aromatic interactions and prepared a library of NFGAIL variants containing fluorinated and iodinated phenylalanine analogues. We used thioflavin T staining, transmission electron microscopy (TEM) and small‐angle X‐ray scattering (SAXS) to study the impact of side‐chain halogenation on NFGAIL amyloid formation kinetics. Our data revealed a synergy between aggregation behavior and hydrophobicity of the phenylalanine residue. This study introduces systematic fluorination as a toolbox to further investigate the nature of the amyloid self‐assembly process. KW - Small-angle X-ray scattering KW - SAXS KW - Nanoparticle KW - Nanostructure KW - Peptide KW - Amyloid PY - 2020 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-518632 DO - https://doi.org/10.1002/cbic.202000373 VL - 21 IS - 24 SP - 3544 EP - 3554 PB - Wiley CY - Weinheim AN - OPUS4-51863 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Stephan, Ina A1 - Heidrich, Gabriele A1 - Stegemann, A. T1 - Isolation of soil borne bacteria and fungi from treated timber T2 - The 32nd annual meeting of IRG (IRG-WP 50174) N2 - Most research in the last few decades has focused on the development of new strategies to control biological attack and the means to quantify this. Comparatively little work has been done to examine the effect that treated timber might have on its surrounding environment. This presentation will describe a methodology that attempts to detect any changes which might occur in the soil microflora following the introduction of timber treated with a number of chemical agents containing organic active ingredients (a.i.). It was also the purpose to isolate organisms that can break down organic a.i.. A technique was developed to determine whether the presence and nature of a timber preservative influenced the size and composition of a microbial population that colonised timber when buried in soil. Samples of treated timber were incubated in a solution eluted from soil that contained a diverse, viable microbial population. After incubation, the size and composition of the microflora both in the eluate and adhering to the timber was examined. T2 - 32nd Annual meeting of IRG, The International Research Group on Wood Preservation CY - Nara, Japan DA - 2001-05-20 KW - Wood preservatives KW - Organic active ingredients KW - Isolation method KW - Soil microorganisms KW - Environmental impact KW - Biodegradation PY - 2001 UR - http://www.irg-wp.com/irgdocs/details.aspx?id=631b6758-939a-43fd-bd68-dea2a2b7cc46 IS - 50174 PB - IRG Secretariat CY - Stockholm AN - OPUS4-1198 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -