TY - CONF A1 - Schneider, Rudolf T1 - Bestimmung von anthropogenen Markerkonzentrationen für die SARS-CoV-2 Quantifizierung mittels eines Hochdurchsatzverfahrens (ELISA) N2 - Im Vorhaben „MARKERIA - Bestimmung von anthropogenen Markerkonzentrationen für die SARS-CoV-2 Quantifizierung mittels eines Hochdurchsatzverfahrens (ELISA)“ geht es um die Bestimmung von Carbamazepin, Diclofenac, Koffein und einer endogenen Gallensäure als potentielle Marker für die Kalibrierung der SARS-CoV-2 Quantifizierung im Abwasser. Der Vortrag wurde gehalten im Rahmen der 22. Sitzung des Begleitkreises des übergeordneten Projektes ESI-CorA. Er gab den Projektstand wieder, den die BAM bis dahin erzielt hatte. Die Marker waren in sämtlichen untersuchten Abwasser-Zulaufproben enthalten und zeigten spezifische Trends. Weitere Korrelationen müsse die weitergehende Datenauswertung ergeben.A T2 - 22. Sitzung des Begleitkreises des Projektes ESI-CorA CY - Online meeting DA - 13.02.2023 KW - Wastewater-based epidemiology KW - ELISA KW - Abwasser KW - Antikörper KW - Antibody PY - 2023 AN - OPUS4-59205 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Lima, D.L.D. A1 - Silva, C.P. A1 - Schneider, Rudolf A1 - Otero, M. A1 - Esteves, V.I. T1 - Application of dispersive liquid-liquid microextraction for estrogens' quantification by enzyme-linked immunosorbent assay N2 - Estrogens, such as 17β-estradiol (E2) and 17α-ethinylestradiol (EE2), are the major responsible for endocrine-disrupting effects observed in aquatic environments due to their high estrogenic potency, even at concentrations ranging from pg L-1 to ng L-1. Thus, it is essential to develop analytical methodologies suitable for monitoring their presence in water samples. Dispersive liquid–liquid microextraction (DLLME) was used as a pre-concentration step prior to the quantification of E2 and EE2 by enzyme-linked immunosorbent assay (ELISA). First, an evaluation of the effect of DDLME on the E2 and EE2 ELISA calibration curves was performed. Since the extraction procedure itself had an influence on the ELISA optical density (OD), it became necessary to subject, not only the samples, but also all the standards to the DLLME process. Working ranges were determined, being between 1.2 and 8000 ng L-1, for E2, and between 0.22 and 1500 ng L-1, for EE2. The influence of organic matter, both in the extraction and quantification, was evaluated and it was observed that its presence in the solution did not affect considerably the calibration curve. Recovery rates were also determined, ranging from 77% to 106% for ultrapure water and from 104% to 115% for waste water samples, the most complex ones in what concerns matrix effects. Results obtained when applying the proposed method to real water samples can be considered quite satisfying. Moreover, the obtained working ranges encompass values generally reported in literature, confirming the practical use of the method for environmental samples. KW - DLLME KW - ELISA KW - Water samples KW - Environment KW - 17beta-estradiol KW - 17alpha-ethinylestradiol PY - 2014 DO - https://doi.org/10.1016/j.talanta.2014.02.069 SN - 0039-9140 VL - 125 SP - 102 EP - 106 PB - Elsevier CY - Amsterdam AN - OPUS4-30614 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Carl, Peter A1 - Ramos, Inês I. A1 - Segundo, Marcela A. A1 - Schneider, Rudolf T1 - Antibody conjugation to carboxyl-modified microspheres through N-hydroxysuccinimide chemistry for automated immunoassay applications: A general procedure N2 - Immunochemical techniques are the workhorse for sample enrichment and detection of a large variety of analytes. In contrast to classical microtiter plate-based assays, microparticles are a next generation solid support, as they promote automation of immunoassays using flow-based techniques. Antibody immobilization is a crucial step, as these reagentsmare expensive, and inefficient coupling can result in low sensitivities. This paper proposes a general procedure for efficient immobilization of antibodies onto TentaGel particles, via Nhydroxysuccinimide chemistry. The goal was the preparation of solid supports with optimum immunorecognition, while increasing the sustainability of the process. The influence of buffer composition, activation and coupling time, as well as the amount of antibody on the immobilization efficiency was investigated, resorting to fluorophore-labeled proteins and fluorescence imaging. Buffer pH and activation time are the most important parameters for efficient coupling. It is demonstrated, that the hydrolysis of N-hydroxysuccinimide esters occurs at similar rates as in solution, limiting the utilizable time for coupling. Finally, applicability of the generated material for automated affinity extraction is demonstrated on the mesofluidic platform lab-on-valve. KW - Mesofluidics KW - Bead injections KW - ELISA KW - Carbamazepine KW - Biomarkers PY - 2019 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-483919 DO - https://doi.org/10.1371/journal.pone.0218686 SN - 1932-6203 VL - 14 IS - 6 SP - e0218686, 1 EP - 18 PB - Public Library of Science CY - San Francisco, California, USA AN - OPUS4-48391 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Analytical Insights at the Point-Of-Need By Immunoanalytical Technologies N2 - A vast number of emerging pollutants is being detected in the environment. Another lingering problem are health-threatening contaminants, such as mycotoxins, that deteriorate food and feed. Analytical methods, suitable for trace analysis, are needed that are desirably also fast, inexpensive and, if possible, robust, and portable. Immunoanalytical, i.e., anti-body-based technologies, which are available in a broad range of formats, can be profitably used here to analyze for the distribution and the concentration trends of contaminants. Some of these formats are single-analyte but high-throughput methods. To use them wisely, indicator substances or sum parameters must be established to be used in screening approaches. Other methods are suitable to be performed on portable in-strumentation in the field (on-site), more precisely at the point-of-need. Furthermore, there are array technologies that allow for parallel analysis of several analytes of interest (multiplexing). The talk reports on methods and data for compounds con-sidered as anthropogenic contamination markers (the antiepileptic carbamazepine, the analgesic diclofenac, the anti-histaminic cetirizine, the steroid hormone estrone, the antimicrobial amoxicillin, the stimulants caffeine and cocaine, the endocrine disruptor bisphenol A, and a bile acid). Some work on mycotoxins (ochratoxin A and ergot alkaloids) is also presented. It is demonstrated that antibody-based technolo-gies have a huge potential to gain analytical insights at the point-of-need. T2 - Virtual International Symposium on Immunoanalytical Technologies for Environmental Monitoring and Human Health CY - Online meeting DA - 10.12.2021 KW - Biosensoren KW - Schnelltest KW - ELISA KW - Lateral Flow Assay PY - 2021 AN - OPUS4-54037 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CHAP A1 - Carvalho, José Joao A1 - Schneider, Rudolf ED - Preedy, V.R. T1 - Analysis of caffeine by immunoassay KW - Immunoassay KW - ELISA KW - Koffein KW - Getränke KW - Trinkwasser KW - Oberflächengewässer KW - Abwasser KW - Speichel PY - 2012 SN - 978-1-84973-367-0 SN - 2045-1695 N1 - Serientitel: Food and nutritional components in focus – Series title: Food and nutritional components in focus VL - 2 IS - Chapter 12 SP - 213 EP - 229 PB - RSC Publ. AN - OPUS4-26530 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Carvalho, José Joao A1 - Weller, Michael G. A1 - Panne, Ulrich A1 - Schneider, Rudolf T1 - A highly sensitive caffeine immunoassay based on a monoclonal antibody KW - Caffeine KW - ELISA KW - Antibody KW - LC–MS–MS KW - Water KW - Wastewater marker PY - 2010 DO - https://doi.org/10.1007/s00216-010-3506-1 SN - 1618-2642 SN - 1618-2650 VL - 396 IS - 7 SP - 2617 EP - 2628 PB - Springer CY - Berlin AN - OPUS4-22651 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -