TY - CONF A1 - Schneider, Rudolf A1 - Hoffmann, Holger T1 - HR-MS non-target analysis for transformation products of emerging organic contaminants in waste water fractions pre-screened by ELISA N2 - The talk introduces the technique of LC-ELISA: fractionating pre-concentrated water samples, analyzing them for containing compounds that bind to hapten-selective antibodies followed by high-Resolution mass spectrometry non-target analysis of "positive" fractions for Transformation products of emerging organic contaminants. T2 - SETAC 2018 CY - Rom, Italy DA - 13.05.2018 KW - Umweltschadstoffe KW - LC-ELISA KW - Hochauflösende Massenspektrometrie KW - LC-MS/MS PY - 2018 AN - OPUS4-45270 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Immunoanalytical methods for screening and monitoring in environmental analysis N2 - A vast number of emerging pollutants has been detected in the environment over the last decades. Analytical methods suitable for trace analysis are needed that are desirably also fast, inexpensive and, if possible, robust and portable. Immunoanalytical methods which are available in a broad range of formats, can be profitably used here to analyze for the distribution and the trends of concentration levels of contaminants in the environment. Some of these formats are single-analyte but high-throughput methods. In order to use them wisely, indicator substances, sometimes called anthropogenic markers, should be selected and used in screening approaches. Other methods are suitable to be performed on portable instrumentation in the field (on-site) or in facilities such as wastewater treatment plants for on-line monitoring. Furthermore, there are the socalled array technologies that allow for parallel analysis of several analytes of interest (multiplexing). The microtiter-plate based ELISA (Enzyme-linked Immunosorbent Assay) is the method of choice for the analysis of a large number of samples [1]. ELISA screening data for anthropogenic markers such as the antiepileptic carbamazepine, the analgesic diclofenac, the anti-histaminic cetirizine, the steroid hormone estrone, the antimicrobial sulfamethoxazole, the stimulants caffeine and cocaine, the priority pollutant bisphenol A, and the bile acid isolithocholic acid [2] are presented. For on-site screening and monitoring, simpler formats, like mix-and-read assays, e.g. the Fluorescence Polarization Immunoassay (FPIA) or Lateral-flow Immunoassays (LFIA) are more suitable tools. Electrochemical formats run on portable devices provide additional advantages as no light source is required. Some examples are presented and discussed. The suitability of multi-analyte formats such as immunomicroarrays depends on the choice of a signal-producing system that provides small uncertainties and good reproducibility of the measurements. Biochip (“flat”) arrays read out on slide scanners and bead-based (“suspension”) arrays read out in flow cytometers are two options and show their distinct pros and cons. Altogether these approaches show the great potential immunoanalytical methods provide for the screening for environmental contaminants in the aquatic environment. T2 - analítica 2018 CY - Porto, Portugal DA - 26.03.2018 KW - Carbamazepine KW - Caffeine KW - Diclofenac KW - LC-ELISA KW - Fluorescence PY - 2018 AN - OPUS4-44691 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf A1 - Bell, Jérémy A1 - Rurack, Knut A1 - Abdelshafi, N. A. T1 - Microfluidic electrochemical lab-on-chip immunosensor for ultrasensitive analysis of cocaine in water, saliva, and urine N2 - A sensitive microfluidic assay for the detection of cocaine in different matrices. T2 - 2nd European Biosensor Symposium CY - Florenz, Italy DA - 18.02.2019 KW - Cocaine KW - Lab-on-chip KW - Microfluidic KW - Electrochemical PY - 2019 AN - OPUS4-48320 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Screening the environment for emerging pollutants by antibody-based methods N2 - Immunoanalytical methods are excellent tools for analysis of the environment with respect to pollutants T2 - ENEQUI Encontro Nacional dos Estudantes de Química CY - Lisbon, Portugal DA - 22.03.2019 KW - Immunoassay KW - Schadstoffe KW - Antikörper PY - 2019 AN - OPUS4-48321 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Immunoanalytical platforms for on-site environmental health and food safety testing N2 - An overview of methods developed in the division. T2 - FoodSmartphone.eu - Online Symposium CY - Online meeting DA - 25.11.2020 KW - Umweltanalytik KW - Lebensmittelanalytik KW - Immunoassay KW - Biosensor PY - 2020 AN - OPUS4-51841 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Portable, antikörperbasierte Analysenverfahren für die Schadstofferfassung im Wasserkreislauf N2 - Die Wasseranalytik wird bestimmt von zwei Hauptbedarfen, einerseits der Erfassung der Gewässergüte und Grundwassergüte als Basis für die Bereitstellung einwandfrei-en Trinkwassers und zum anderen des Monitorings der Wasserqualität in den Prozes-sen der Wasseraufbereitung und der Abwasserreinigung. Für beide Anwendungsbe-reiche ist eine laborbasierte Analytik eher hinderlich, werden die Analysenergebnisse doch eigentlich vor Ort benötigt, um etwa Beprobungskampagnen rasch anpassen zu können. Desweiteren werden die Daten auch schnell benötigt, um zeitnah in die technischen Prozesse einzugreifen. Organische Kontaminanten werden meist über chromatographische Verfahren, häufig gekoppelt mit einer massenspektrometrischen Detektion, in instrumentelle Labormethoden erfasst und die hochauflösende Massen-spektrometrie und die Non-target-Analytik haben viel zum Wissen über das Vorkom-men, insbesondere von sog. „emerging contaminants“, beigetragen, sowie zum Ver-ständnis der beteiligten Eintragspfade und Abbauwege. Moderne, portable Analy-senmethoden und insbesondere Sensoren werden zukünftig aber ihren Platz in der Schadstofferfassung im Wasserkreislauf einnehmen. Dies gilt insbesondere für anti-körperbasierte Analysenverfahren, da erst diese oft die nötige Selektivität und Sensitivität in die Detektionsmethoden einbringen. T2 - Wasser 2021 - Jahrestagung der Wasserchemischen Gesellschaft in der GDCh CY - Online meeting DA - 10.05.2021 KW - Immunoassay KW - Biosensoren KW - Schnelltest KW - Elektrochemisch PY - 2021 AN - OPUS4-54051 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Environmental monitoring by high-throughput immunoanalytical methods and portable devices N2 - A vast number of emerging pollutants has been detected in the environment over the last decades. Analytical methods suitable for trace analysis are needed that are desirably also fast, inexpensive and, if possible, robust and portable. Immunoanalytical, i.e. antibody-based, methods which are available in a broad range of formats, can be profitably used here to analyse for the distribution and the trends of concentration levels of contaminants in the environment. Some of these formats are single-analyte but high-throughput methods. In order to use them wisely, indicator substances, sometimes called anthropogenic markers, should be selected and used in screening approaches. Other methods are suitable to be performed on portable instrumentation in the field (on-site) or in facilities such as wastewater treatment plants for on-line monitoring. Furthermore, there are array technologies that allow for parallel (multiplex) analysis of several analytes of interest. The microtiter-plate based ELISA (Enzyme-linked Immunosorbent Assay) is the method of choice for the analysis of a large number of samples [1]. ELISAs are available to monitor for anthropogenic markers such as the antiepileptic carbamazepine, the analgesic diclofenac, the antihistaminic cetirizine, the steroid hormone estrone, the antimicrobial sulfamethoxazole, the stimulants caffeine and cocaine, the priority pollutant bisphenol A, and the bile acid isolithocholic acid. For on-site screening and monitoring, simpler formats, like mix-and-read assays, e.g. the Fluorescence Polarization Immunoassay (FPIA) [2] or Lateral-flow Immunoassays (LFIA) [3] are more suitable tools, the latter based on dipsticks or cassettes, that is why they are also called pregnancy test-like assays. The suitability of multi-analyte formats such as immunomicroarrays depends on the choice of a signal-producing system that provides small uncertainties and good reproducibility of the measurements. Bead-based (“suspension”) arrays read out in flow cytometers are a powerful platform for multiplex assays [4]. Electrochemical formats run on portable devices provide additional advantages as no light source is required. They are most promising for stand-alone analysers and biosensors T2 - European Biosensor Symposium 2021 CY - Online meeting DA - 09.03.2021 KW - Biosensoren KW - Schnelltest KW - Elektrochemisch KW - Immunoassay PY - 2021 AN - OPUS4-54052 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Analytical Insights at the Point-Of-Need By Immunoanalytical Technologies N2 - A vast number of emerging pollutants is being detected in the environment. Another lingering problem are health-threatening contaminants, such as mycotoxins, that deteriorate food and feed. Analytical methods, suitable for trace analysis, are needed that are desirably also fast, inexpensive and, if possible, robust, and portable. Immunoanalytical, i.e., anti-body-based technologies, which are available in a broad range of formats, can be profitably used here to analyze for the distribution and the concentration trends of contaminants. Some of these formats are single-analyte but high-throughput methods. To use them wisely, indicator substances or sum parameters must be established to be used in screening approaches. Other methods are suitable to be performed on portable in-strumentation in the field (on-site), more precisely at the point-of-need. Furthermore, there are array technologies that allow for parallel analysis of several analytes of interest (multiplexing). The talk reports on methods and data for compounds con-sidered as anthropogenic contamination markers (the antiepileptic carbamazepine, the analgesic diclofenac, the anti-histaminic cetirizine, the steroid hormone estrone, the antimicrobial amoxicillin, the stimulants caffeine and cocaine, the endocrine disruptor bisphenol A, and a bile acid). Some work on mycotoxins (ochratoxin A and ergot alkaloids) is also presented. It is demonstrated that antibody-based technolo-gies have a huge potential to gain analytical insights at the point-of-need. T2 - Virtual International Symposium on Immunoanalytical Technologies for Environmental Monitoring and Human Health CY - Online meeting DA - 10.12.2021 KW - Biosensoren KW - Schnelltest KW - ELISA KW - Lateral Flow Assay PY - 2021 AN - OPUS4-54037 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Advanced Materials in antibody-based analytical techniques for environmental analysis N2 - Antibody-based analytical techniques have gained increasing importance in environmental analysis. Rapid assays, portable devices and formats that require a limited number of steps have a high potential for on-site analysis in outdoor environments or in environmentally relevant facilities such as wastewater treatment plants. Many of these methods have become more sensitive and more versatile due to new, advanced materials, such as novel particles, specifically prepared surfaces, new labels, specifically coated electrodes etc. The talk shows some of our works in this context. T2 - V Analytical Chemistry Symposium: Advanced Materials for Environmental Applications CY - Online meeting DA - 24.11.2021 KW - Biosensoren KW - Immunoassay KW - Schnelltest KW - Vor-Ort-Analytik KW - Portable Analytik PY - 2021 AN - OPUS4-54038 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Rudolf T1 - Analytical insights at the point-of-need with antibody-based methods N2 - A vast number of emerging pollutants is being detected in the environment. Another lingering problem are health-threatening contaminants, such as mycotoxins, that deteriorate food and feed. Analytical methods, suitable for trace analysis, are needed that are desirably also fast, inexpensive and, if possible, robust, and portable. Immunoanalytical, i.e., antibody-based methods, which are available in a broad range of formats, can be profitably used here to analyze for the distribution and the concen-tration trends of contaminants. Some of these formats are single-analyte but high-throughput methods. To use them wisely, indicator substances or sum parameters are being established and used in screening approaches. Other methods are suitable to be performed on portable instrumentation in the field (on-site), more precisely at the point-of-need. Furthermore, there are array technologies that allow for parallel analysis of several analytes of interest (multiplexing). The talk reports on methods and data for compounds considered as anthropogenic contamination markers (the antiepileptic carbamazepine, the analgesic diclofenac, the anti-histaminic cetirizine, the steroid hormone estrone, the antimicrobial amoxicillin, the stimulants caffeine and cocaine, the endo-crine disruptor bisphenol A, and a bile acid). Some work on mycotoxins (ochratoxin A and ergot alka-loids) is also presented. It is demonstrated that antibody-based approaches have a huge potential to gain analytical insights at the point-of-need. T2 - empa - Seminar of the Laboratory of Advanced Analytical Technologies CY - Online meeting DA - 24.11.2021 KW - Biosensoren KW - Point-of-Care KW - Schnelltests KW - Vor-Ort-Analytik PY - 2021 AN - OPUS4-54040 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -