TY - JOUR A1 - Klingbeil, Patrick A1 - Vogl, Jochen A1 - Pritzkow, Wolfgang A1 - Riebe, Gundel A1 - Müller, J. T1 - Comparative studies on the certification of reference materials by ICPMS and TIMS using isotope dilution procedures N2 - A comparison of different isotope dilution mass spectrometric (IDMS) procedures using inductively coupled plasma mass spectrometry (ICPMS) and thermal ionization mass spectrometry (TIMS) was carried out to examine the degree of equivalence between the used procedures in terms of requirements for reference material certification. The comparison was based on the measurement results and their uncertainties. The sample used in this study is a pure zinc metal to be certified by the Bureau Communie de Référence (BCR) for amount contents of different trace elements. This study focuses on cadmium and thallium. The TIMS values contributed to the certified values. To guarantee identical conditions as far as possible for the procedures under investigation, the samples were split into subsamples after spiking and digestion took place. Thus, every IDMS procedure started with an identical set of samples. In total, four different IDMS procedures and one external calibration procedure using internal standardization as an example of routine analysis were applied. The IDMS procedures divide in a group with and a group without trace/matrix separation. Multicollector TIMS (TI-MC-MS) and multicollector ICPMS (ICP-MC-MS) were used in combination with trace/matrix separation, whereas quadrupole ICPMS (ICP-QMS) and ICP-MC-MS were also applied to nonseparated samples. All IDMS results agree well within their combined uncertainties, while some results from the external calibration procedure do not. IDMS results obtained by ICPMS without separation are comparable to those obtained by TI-MC-MS with separation regarding precision and accuracy. The smallest uncertainties were achieved using ICP-MC-MS in combination with trace/matrix separation. KW - ICP-MS KW - TIMS KW - IDMS KW - multi-collector KW - uncertainty budget KW - Certification KW - reference material PY - 2001 U6 - https://doi.org/10.1021/ac001278c SN - 0003-2700 SN - 1520-6882 VL - 73 IS - 8 SP - 1881 EP - 1888 PB - American Chemical Society CY - Washington, DC AN - OPUS4-909 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Bohn, T. A1 - Walczyk, Th. A1 - Davidsson, L. A1 - Pritzkow, Wolfgang A1 - Klingbeil, Patrick A1 - Vogl, Jochen A1 - Hurrell, R.F. T1 - Comparison of urinary monitoring, faecal monitoring and erythrocyte analysis of stable isotope labels to determine magnesium absorption in human subjects KW - Magnesium absorption KW - Stable isotopes KW - Faecal monitoring KW - Urinary monitoring KW - Erythrocytes PY - 2004 SN - 0007-1145 SN - 1475-2662 VL - 58 SP - 113 EP - 120 PB - CABI Publ. CY - Wallingford AN - OPUS4-3362 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pritzkow, Wolfgang A1 - Klingbeil, Patrick T1 - Messtechnik T2 - 1. Workshop "Isotopenverdünnungsanalyse mit ICP-MS" CY - Berlin, Germany DA - 2002-11-11 PY - 2002 AN - OPUS4-2989 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Dudek, Gabriele A1 - Pritzkow, Wolfgang A1 - Riebe, Gundel A1 - Görner, Wolf A1 - Alber, D. T1 - Characterization of neutron transmuted zinc traces in pure copper materials by isotope dilution mass spectrometry N2 - The neutron transmutation doping (NTD) of highly pure copper with zinc was investigated as a promising means of achieving controlled gradation of the zinc content in the range 1-20 wg g-1. The doping process leads to the enrichment of two stable isotopes 64Zn and 66Zn in a ratio which differs from that of natural isotopic distribution. Mass spectrometric investigations by thermal ionization mass spectrometry (TIMS) were performed to validate the results obtained by gamma spectrometry. The investigations included both determination of the isotopic ratios of the doped zinc isotopes and the analysis of the accumulated zinc contents by isotope dilution (ID) analysis. Thereby a sample-specific correction of the blank could be performed because the isotope 68Zn was not influenced, because of the transmutation process. The results obtained by TIMS prove the strict proportionality of the doped zinc content, in the range 5 to 20 wg g-1, to the neutron fluence. Comparison with gamma spectrometric results showed a very good agreement within the uncertainties. PY - 2001 U6 - https://doi.org/10.1007/s002160100784 SN - 0937-0633 N1 - Geburtsname von Dudek, Gabriele: Wermann, G. - Birth name of Dudek, Gabriele: Wermann, G. VL - 370 IS - 5 SP - 606 EP - 611 PB - Springer CY - Berlin AN - OPUS4-1145 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Riebe, Gundel A1 - Klingbeil, Patrick A1 - Pritzkow, Wolfgang A1 - Vogl, Jochen T1 - Erfahrungen mit mikrowelleninduzierten Aufschluss-Verfahren für die Elementspurenanalytik T2 - MLS-Tagesseminar CY - Berlin, Deutschland DA - 2001-03-19 PY - 2001 AN - OPUS4-1111 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Riebe, Gundel A1 - Ostermann, Markus A1 - Pritzkow, Wolfgang A1 - Vogl, Jochen T1 - Combination of sophisticated sample digestion methods and highly accurate determination procedures shown for Sulphur in oil and Cadmium and Lead in sediment T2 - Internationale Konferenz "Trends in Sample Preparation 2002" CY - Seggau, Austria DA - 2002-06-30 PY - 2002 AN - OPUS4-1398 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Vogl, Jochen A1 - Klingbeil, Patrick A1 - Pritzkow, Wolfgang A1 - Riebe, Gundel T1 - High accuracy measurements of Fe isotopes using hexapole collision cell MC-ICP-MS and isotope dilution for certification of reference materials N2 - An ICP-MS equipped with collision cell, sector field and multi-collector technology has been applied to developing analytical procedures for Fe in terms of isotope ratio measurements and isotope dilution analysis. Therefore a detailed study on the reduction of molecular interferences by a variety of collision gases (He, Ar, Kr, H2, D2 and N2) was performed. The efficiency of the reduction has been checked by high resolution mass scans. The argon based interferences disturbing the determination of 54Fe and 56Fe could be completely removed when using Faraday detectors. The interferences on mass 57 could be reduced to a level which enables accurate measurements. Also, the 40Ar12C interference, which disturbs the chromium correction, could be completely removed. Reproducibilities of 0.01% could be reached for 54Fe/56Fe isotope ratios and 0.02% for 57Fe/56Fe isotope ratios. Under these measurement conditions a sensitivity of 1.2 V per 50 µg kg–1 Fe is available. This corresponds to 1.5 × 109 cps per mg kg–1. Based on these results highly accurate procedures for Fe isotope ratio determinations were set up, reaching a performance level superior to that of other ICP-MS instruments. Consequently, IDMS procedures were developed on this basis in order to perform certification measurements at the highest metrological level. The first application was the contribution to the Fe reference value by IMEP-12, an interlaboratory comparison with approximately 350 participants worldwide. The analysed Fe mass content is 0.2150 mg kg–1 with its expanded uncertainty (k= 2) of 0.0012 mg kg–1(0.56%), which is ten times less than the uncertainty of the second certification laboratory. Another application was the certification campaign of the two reference materials Tea Leaves and Polish Herbs. The results obtained with the ICP-MS procedure, 542 ± 11 mg kg–1 and 543 ± 12 mg kg–1, were compared with the results analysed by TIMS, 541 ± 12 mg kg–1 and 541 ± 13 mg kg–1, and agree very well within the stated uncertainties (k= 2). The results presented demonstrate quite well the suitability of the developed IDMS procedures for certification or reference measurements. PY - 2003 U6 - https://doi.org/10.1039/b301812a SN - 0267-9477 SN - 1364-5544 VL - 18 SP - 1125 EP - 1132 PB - Royal Society of Chemistry CY - London AN - OPUS4-2739 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pritzkow, Wolfgang A1 - Vogl, Jochen A1 - Riebe, Gundel T1 - A comparative study in the field of soil analysis T2 - 2. Symposium " Trends in sample preparation" CY - Seggauberg, Austria DA - 2004-07-04 PY - 2004 AN - OPUS4-5054 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Knobbe, N. A1 - Vogl, Jochen A1 - Pritzkow, Wolfgang A1 - Panne, Ulrich A1 - Fry, H. A1 - Lochotzke, H.M. A1 - Preiss-Weigert, A. T1 - C and N stable isotope variation in urine and milk of cattle depending on the diet N2 - The stable carbon and nitrogen isotopic composition of urine and milk samples from cattle under different feeding regimes were analysed over a period of six months. The isotope ratios were measured with isotope ratio mass spectrometry (IRMS). The δ13C values of milk and urine were dependent on different feeding regimes based on C3 or C4 plants. The δ13C values are more negative under grass feeding than under maize feeding. The δ13C values of milk are more negative compared to urine and independent of the feeding regime. Under grass feeding the analysed milk and urine samples are enriched in 13C relative to the feed, whereas under maize feeding the 13C/12C ratio of urine is in the same range and milk is depleted in 13C relative to the diet. The difference between the 15N/14N ratios for the two feeding regimes is less pronounced than the 13C/12C ratios. The δ15N values in urine require more time to reach the new equilibrium, whereas the milk samples show no significant differences between the two feeding regimes. KW - Cattle KW - Urine KW - Milk KW - Feeding regime KW - Stable isotope PY - 2006 U6 - https://doi.org/10.1007/s00216-006-0644-6 SN - 1618-2642 SN - 1618-2650 VL - 386 IS - 1 SP - 104 EP - 108 PB - Springer CY - Berlin AN - OPUS4-12746 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pritzkow, Wolfgang A1 - Klingbeil, Patrick A1 - Vogl, Jochen A1 - Walczyk, Th. A1 - Bohn, T. T1 - Bestimmung der Magnesium-Isotopenhäufigkeiten in Urin und Blut T2 - 17. ICP-MS Anwendertreffen und 2nd International Conference on High-resolution Sector Field ICP-MS CY - Vienna, Austria DA - 2001-09-10 PY - 2001 AN - OPUS4-7715 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pritzkow, Wolfgang A1 - Klingbeil, Patrick A1 - Vogl, Jochen A1 - Riebe, Gundel A1 - Berglund, M. A1 - Nelms, S. A1 - Taylor, P.D.P. A1 - Quetel, C. A1 - Wachsmann, M. T1 - Kommerzielle Spike-Lösungen zur Verbesserung der Analytik T2 - 5. Symposium Massenspektrometrische Verfahren der Elementspurenanalyse und 16. ICP-MS Anwendertreffen CY - Jülich, Germany DA - 2000-09-18 PY - 2000 AN - OPUS4-7710 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Riebe, Gundel A1 - Pritzkow, Wolfgang A1 - Vogl, Jochen T1 - Schwermetallbestimmung im Boden KW - Königswasserextraktion KW - Bodenanalyse KW - Schwermetallbestimmung KW - Unsicherheitsvergleiche PY - 2006 SN - 0016-3538 IS - 8 SP - 688 EP - 690 PB - GIT-Verlag CY - Darmstadt AN - OPUS4-13725 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Pritzkow, Wolfgang A1 - Vogl, Jochen A1 - Berger, Achim A1 - Ecker, Klaus A1 - Grötzschel, R. A1 - Klingbeil, Patrick A1 - Persson, L. A1 - Riebe, Gundel A1 - Wätjen, U. T1 - Contribution of ICP-IDMS to the certification of antimony implanted in a silicon wafer - comparison with RBS and INAA results N2 - A thin-layer reference material for surface and near-surface analytical methods was produced and certified. The surface density of the implanted Sb layer was determined by Rutherford backscattering spectrometry (RBS), instrumental neutron activation analysis (INAA), and inductively coupled plasma isotope dilution mass spectrometry (ICP-IDMS) equipped with a multi-collector. The isotopic abundances of Sb (121Sb and 123Sb) were determined by multi-collector ICP-MS and INAA. ICP-IDMS measurements are discussed in detail in this paper. All methods produced values traceable to the SI and are accompanied by a complete uncertainty budget. The homogeneity of the material was measured with RBS. From these measurements the standard uncertainty due to possible inhomogeneities was estimated to be less than 0.78% for fractions of the area increments down to 0.75 mm2 in size. Excellent agreement between the results of the three different methods was found. For the surface density of implanted Sb atoms the unweighted mean value of the means of four data sets is 4.8121016 cm-2 with an expanded uncertainty (coverage factor k=2) of 0.0921016 cm-2. For the isotope amount ratio R (121Sb/123Sb) the unweighted mean value of the means of two data sets is 1.435 with an expanded uncertainty (coverage factor k=2) of 0.006. KW - ICP-MS KW - IDMS KW - Reference material KW - Surface analysis PY - 2001 U6 - https://doi.org/10.1007/s002160100987 SN - 0937-0633 VL - 371 SP - 867 EP - 873 PB - Springer CY - Berlin AN - OPUS4-7220 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Dudek, Gabriele A1 - Alber, D. A1 - Pritzkow, Wolfgang A1 - Riebe, Gundel A1 - Vogl, Jochen A1 - Görner, Wolf T1 - Determination of the beta- branching ratio of 64Cu by mass spectrometric investigations of the decay products in neutron transmuted copper N2 - The ?- branching ratio of 64Cu was determined by investigating the resulting decay products in copper doped by neutron transmutation. The numbers of 64Zn and 64Ni atoms were analyzed using isotope dilution analysis combined with thermal ionization mass spectrometry. A ?- branching ratio of (38.06±0.30)% was obtained, which agrees with the study of Kawada (Appl. Radiat. Isot. 37 (1) (1986) 7) to a higher accuracy. However, our result differs from the value cited in the NUDAT database of (39.0±0.3)%. KW - NTD KW - Copper KW - Zinc KW - Nickel KW - Branching ratio KW - Cu-64 KW - TIMS KW - IDMS KW - Isotope dilution KW - Thermal ionization mass spectrometry KW - Neutron transmutation doping PY - 2002 U6 - https://doi.org/10.1016/S0969-8043(01)00180-4 SN - 0883-2889 SN - 0969-8043 N1 - Geburtsname von Dudek, Gabriele: Wermann, G. - Birth name of Dudek, Gabriele: Wermann, G. VL - 56 SP - 145 EP - 151 PB - Elsevier CY - Amsterdam AN - OPUS4-7221 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Pritzkow, Wolfgang A1 - Vogl, Jochen A1 - Köppen, Robert A1 - Ostermann, Markus T1 - Determination of sulfur isotope abundance ratios for SI-traceable low sulfur concentration measurements in fossil fuels by ID-TIMS N2 - The present work describes the development of an existing TIMS procedure to a reference procedure for low sulfur concentration measurements in fossil fuels. With this enhanced procedure SI-traceable sulfur mass fractions below 10 mg kg-1 can be obtained. The achieved detection limit is approximately 0.2 mg kg-1. The procedure was validated by certified reference materials. The procedure was already applied to candidate reference materials and to samples analysed within projects of the “Comité Consultatif pour la Quantité de Matière – CCQM”. Additionally the influence of the isotopic composition on the results and its corresponding uncertainty was studied. Reference data published in the literature on the isotopic composition of sulfur were assessed. KW - TIMS KW - IDMS KW - Low sulfur KW - Fossil fuel KW - Reference procedure PY - 2005 U6 - https://doi.org/10.1016/j.ijms.2004.10.024 SN - 0168-1176 SN - 0020-7381 SN - 1387-3806 SN - 1873-2798 VL - 242 SP - 309 EP - 318 PB - Elsevier CY - Amsterdam AN - OPUS4-7222 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Ecker, Klaus A1 - Wätjen, U. A1 - Berger, Achim A1 - Persson, L. A1 - Pritzkow, Wolfgang A1 - Radtke, Martin A1 - Riesemeier, Heinrich T1 - RBS, SY-XRF, INAA and ICP-IDMS of antimony implanted in silicon - A multi-method approach to characterize and certify a reference material N2 - A layer of Sb atoms, implanted with an energy of 400 keV and a nominal dose of 5×1016 atoms/cm2 into a high purity silicon wafer, was certified for its areal density (atoms/cm2) using Rutherford backscattering spectrometry (RBS), instrumental neutron activation analysis (INAA) and inductively coupled plasma isotope dilution mass spectrometry (ICP-IDMS) and for its isotope ratio using INAA and ICP-IDMS. Excellent agreement between the results of the different independent methods was found. In the present work, the measurements of the homogeneity of the areal density of Sb, previously determined with RBS in spots having 1 mm diameter, are improved with synchrotron X-ray fluorescence analysis: Higher precision in even smaller sample spots allows to estimate a reduced inhomogeneity of the whole batch of samples of the order of only 0.4%. Thus the uncertainty of the certified value can further be reduced. Down to fractions of a chip with 0.3×0.4 mm2 area, the areal density is now certified as (4.81±0.06)×1016 Sb atoms/cm2, where the expanded uncertainty 0.06 (coverage factor k=2) corresponds to only 1.2%. The relative merits of the different analytical methods are discussed. KW - Certified reference material KW - RBS KW - INAA KW - ICP-IDMS KW - XRF KW - SY-XRF PY - 2002 U6 - https://doi.org/10.1016/S0168-583X(01)01038-2 SN - 0168-583X SN - 1872-9584 VL - 188 IS - 1-4 SP - 120 EP - 125 PB - Elsevier CY - Amsterdam AN - OPUS4-13870 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Vogl, Jochen A1 - Rosner, Martin A1 - Pritzkow, Wolfgang T1 - The need for new isotope reference materials N2 - Isotope reference materials are needed to calibrate and validate analytical procedures used for the determination of isotope amount ratios, procedurally defined isotope ratios or so-called δ values. In contrast to the huge analytical progress in isotope ratio analytics, the production of isotope reference materials has not kept pace with the increasing needs of isotope analysts. Three representative isotope systems are used to explain the technical and non-technical difficulties and drawbacks, on one hand, and to demonstrate what can be achieved at its best, on the other hand. A clear statement is given that new isotope reference materials are needed to obtain traceable and thus comparable data, which is essential for all kinds of isotope research. The range of available isotope reference materials and δ reference materials should be increased and matrix reference materials certified for isotope compositions or δ values, which do not exist yet, should be provided. KW - Isotope reference materials KW - Delta reference materials KW - Synthetic isotope mixtures KW - Mass spectrometry KW - Comparability KW - Traceability KW - Measurement uncertainty PY - 2013 U6 - https://doi.org/10.1007/s00216-012-6605-3 SN - 1618-2642 SN - 1618-2650 VL - 405 IS - 9 SP - 2763 EP - 2770 PB - Springer CY - Berlin AN - OPUS4-28169 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Vogl, Jochen A1 - Paz, B. A1 - Koenig, Maren A1 - Pritzkow, Wolfgang T1 - A modified lead-matrix separation procedure shown for lead isotope analysis in Trojan silver artefacts as an example N2 - A modified Pb–matrix separation procedure using NH4HCO3 solution as eluent has been developed and validated for determination of Pb isotope amount ratios by thermal ionization mass spectrometry. The procedure is based on chromatographic separation using the Pb·Spec resin and an in-house-prepared NH4HCO3 solution serving as eluent. The advantages of this eluent are low Pb blanks (<40 pgmL-1) and the property that NH4HCO3 can be easily removed by use of a heating step (>60 °C). Pb recovery is >95 % for water samples. For archaeological silver samples, however, the Pb recovery is reduced to approximately 50 %, but causes no bias in the determination of Pb isotope amount ratios. The validated procedure was used to determine lead isotope amount ratios in Trojan silver artefacts with expanded uncertainties (k=2) <0.09 %. KW - Analyte-matrix separation KW - Mass spectrometry KW - Pb isotope ratio thermal ionization mass spectrometry KW - Archaeometry PY - 2013 U6 - https://doi.org/10.1007/s00216-012-6323-x SN - 1618-2642 SN - 1618-2650 VL - 405 IS - 9 SP - 2995 EP - 3000 PB - Springer CY - Berlin AN - OPUS4-28170 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Weißhaupt, Petra A1 - Pritzkow, Wolfgang A1 - Noll, Matthias T1 - Nitrogen sources of oligoporus placenta and trametes versicolor evaluated in a 2³ experimental plan N2 - Four full-factorial 2³ experimental plans were applied to evaluate the nitrogen (N) sources of Oligoporus placenta and Trametes versicolor and their interaction with the atmospheric N2-assimilating bacterium Beijerinckia acida. The effects of N from peptone, of sapwood and of N from gaseous N2 on fungal, bacterial and fungal–bacterial activity were investigated. The activities were determined by quantification of biomass, formation of CO2, consumption of O2 and laccase activity. The significance of each effect was tested according to t-test recommendation. The activity of both fungi was enhanced by peptone rather than sapwood or gaseous N2. Nevertheless, comparative studies under an N2-free gas mixture as well as under air revealed that the presence of N2 affected bacterial growth and bacterial–fungal cocultivations. Elemental analysis isotope ratio mass spectrometry (IRMS) of the bacterial and fungal biomass enabled estimation of N transfer and underlined gaseous N2 as requisite for fungal–bacterial interactions. Combining full-factorial experimental plans with an analytical set-up comprising gas chromatography, IRMS and enzymatic activity allowed synergistic effects to be revealed, fungal N sources to be traced, and symbiotic fungal–bacterial interactions to be investigated. KW - Basidiomycetes KW - Diazotroph KW - Full-factorial experimental plan KW - Fungal–bacterial interaction KW - Nitrogen KW - Wood decomposition PY - 2012 U6 - https://doi.org/10.1016/j.funbio.2011.10.002 SN - 1878-6146 SN - 1878-6162 VL - 116 IS - 1 SP - 81 EP - 89 PB - Elsevier Science CY - Amsterdam [u.a.] AN - OPUS4-25211 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schmalenberger, A. A1 - Pritzkow, Wolfgang A1 - Ojeda, J.J. A1 - Noll, Matthias T1 - Characterization of main sulfur source of wood-degrading basidiomycetes by S K-edge X-ray absorption near edge spectroscopy (XANES) N2 - The main wood degraders in aerobic terrestrial ecosystems belong to the white- and brown-rot fungi, where their biomass can be created on wood decay only. However, total sulfur (S) concentration in wood is very low and only little is known about the different sulfur compounds in wood today. Sulfur-starved brown-rot fungi Gloeophyllum trabeum and Oligoporus placenta were incubated on sterilized pine wood blocks whereas Lentinus cyathiformis and the white-rot fungi Trametes versicolor were incubated on sterilized beech wood blocks. After 19 weeks of incubation, the S oxidation status was analyzed in wood, in degraded wood, and in biomass of wood-degrading fungi by synchrotron based S K-edge XANES, and total S and sulfate were quantified. Total sulfur and sulfate content in pine wood blocks were approximately 50 and 1 µg g-1, respectively, while in beech wood approximately 100 and 20 µg g-1 were found, respectively. Sulfur in beech was dominated by sulfate-esters. In contrast, pine wood also contained larger amounts of reduced S. Three out of four selected fungi caused a reduction of the S oxidation state in wood from oxidized S (sulfate-ester, sulfate) to intermediate S (sulfonate, sulfoxide) or reduced S (thiols, e.g., proteins, peptides, enzyme cofactors). Only O. placenta shifted thiol to sulfonate. Growth experiments of these fungi on selective minimal media showed that in particular cysteine (thiol), sulfonates, and sulfate enhanced total mycelium growth. Consequently, wood-degrading fungi were able to utilize a large variety of different wood S sources for growth but preferentially transformed in vivo sulfate-esters and thiol into biomass structures. KW - Basidiomycetes KW - Fungi KW - S K-edge X-ray absorption near edge spectroscopy (XANES) KW - Sulfur oxidation status KW - Sulfate-esters PY - 2011 U6 - https://doi.org/10.1016/j.ibiod.2011.08.013 SN - 0964-8305 VL - 65 IS - 8 SP - 1215 EP - 1223 PB - Elsevier CY - Barking AN - OPUS4-27546 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -