TY - JOUR A1 - Vogl, Jochen A1 - Paz, B. A1 - Koenig, Maren A1 - Pritzkow, Wolfgang T1 - A modified lead-matrix separation procedure shown for lead isotope analysis in Trojan silver artefacts as an example N2 - A modified Pb–matrix separation procedure using NH4HCO3 solution as eluent has been developed and validated for determination of Pb isotope amount ratios by thermal ionization mass spectrometry. The procedure is based on chromatographic separation using the Pb·Spec resin and an in-house-prepared NH4HCO3 solution serving as eluent. The advantages of this eluent are low Pb blanks (<40 pgmL-1) and the property that NH4HCO3 can be easily removed by use of a heating step (>60 °C). Pb recovery is >95 % for water samples. For archaeological silver samples, however, the Pb recovery is reduced to approximately 50 %, but causes no bias in the determination of Pb isotope amount ratios. The validated procedure was used to determine lead isotope amount ratios in Trojan silver artefacts with expanded uncertainties (k=2) <0.09 %. KW - Analyte-matrix separation KW - Mass spectrometry KW - Pb isotope ratio thermal ionization mass spectrometry KW - Archaeometry PY - 2013 U6 - https://doi.org/10.1007/s00216-012-6323-x SN - 1618-2642 SN - 1618-2650 VL - 405 IS - 9 SP - 2995 EP - 3000 PB - Springer CY - Berlin AN - OPUS4-28170 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Jakobs, Désirée A1 - Mathies, Helena A1 - Abraham, W.-R. A1 - Pritzkow, Wolfgang A1 - Stephan, Ina A1 - Noll, Matthias T1 - Biodegradation of a biocide (Cu-N-cyclohexyldiazenium dioxide) component of a wood preservative by a defined soil bacterial community N2 - The wood protection industry has refined their products from chrome-, copper-, and arsenate-based wood preservatives toward solely copper-based preservatives in combination with organic biocides. One of these is Cu-HDO, containing the chelation product of copper and N-cyclohexyldiazenium dioxide (HDO). In this study, the fate of isotope-labeled (13C) and nonlabeled (12C) Cu-HDO incorporated in wood sawdust mixed with soil was investigated. HDO concentration was monitored by high-pressure liquid chromatography. The total carbon and the δ13C content of respired CO2, as well as of the soil-wood-sawdust mixture, were determined with an elemental analyzer-isotopic ratio mass spectrometer. The concentration of HDO decreased significantly after 105 days of incubation, and after 24 days the 13CO2 concentration respired from soil increased steadily to a maximum after 64 days of incubation. Phospholipid fatty acid-stable isotope probing (PFA-SIP) analysis revealed that the dominant PFAs C19:0d8,9, C18:0, C18:1ω7, C18:2ω6,9, C17:1d7,8, C16:0, and C16:1ω7 were highly enriched in their δ13C content. Moreover, RNA-SIP identified members of the phylum Acidobacteria and the genera Phenylobacterium and Comamonas that were assimilating carbon from HDO exclusively. Cu-HDO as part of a wood preservative effectively decreased fungal wood decay and overall microbial respiration from soil. In turn, a defined bacterial community was stimulated that was able to metabolize HDO completely. KW - 16S rRNA KW - Biocide KW - PLFA KW - Stable isotope probing KW - Soil KW - Wood preservative PY - 2010 U6 - https://doi.org/10.1128/AEM.01092-10 SN - 0099-2240 VL - 76 IS - 24 SP - 8076 EP - 8083 PB - American Society for Microbiology CY - Washington, DC [u.a.] AN - OPUS4-22688 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Knobbe, N. A1 - Vogl, Jochen A1 - Pritzkow, Wolfgang A1 - Panne, Ulrich A1 - Fry, H. A1 - Lochotzke, H.M. A1 - Preiss-Weigert, A. T1 - C and N stable isotope variation in urine and milk of cattle depending on the diet N2 - The stable carbon and nitrogen isotopic composition of urine and milk samples from cattle under different feeding regimes were analysed over a period of six months. The isotope ratios were measured with isotope ratio mass spectrometry (IRMS). The δ13C values of milk and urine were dependent on different feeding regimes based on C3 or C4 plants. The δ13C values are more negative under grass feeding than under maize feeding. The δ13C values of milk are more negative compared to urine and independent of the feeding regime. Under grass feeding the analysed milk and urine samples are enriched in 13C relative to the feed, whereas under maize feeding the 13C/12C ratio of urine is in the same range and milk is depleted in 13C relative to the diet. The difference between the 15N/14N ratios for the two feeding regimes is less pronounced than the 13C/12C ratios. The δ15N values in urine require more time to reach the new equilibrium, whereas the milk samples show no significant differences between the two feeding regimes. KW - Cattle KW - Urine KW - Milk KW - Feeding regime KW - Stable isotope PY - 2006 U6 - https://doi.org/10.1007/s00216-006-0644-6 SN - 1618-2642 SN - 1618-2650 VL - 386 IS - 1 SP - 104 EP - 108 PB - Springer CY - Berlin AN - OPUS4-12746 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Schmalenberger, A. A1 - Pritzkow, Wolfgang A1 - Ojeda, J.J. A1 - Noll, Matthias T1 - Characterization of main sulfur source of wood-degrading basidiomycetes by S K-edge X-ray absorption near edge spectroscopy (XANES) N2 - The main wood degraders in aerobic terrestrial ecosystems belong to the white- and brown-rot fungi, where their biomass can be created on wood decay only. However, total sulfur (S) concentration in wood is very low and only little is known about the different sulfur compounds in wood today. Sulfur-starved brown-rot fungi Gloeophyllum trabeum and Oligoporus placenta were incubated on sterilized pine wood blocks whereas Lentinus cyathiformis and the white-rot fungi Trametes versicolor were incubated on sterilized beech wood blocks. After 19 weeks of incubation, the S oxidation status was analyzed in wood, in degraded wood, and in biomass of wood-degrading fungi by synchrotron based S K-edge XANES, and total S and sulfate were quantified. Total sulfur and sulfate content in pine wood blocks were approximately 50 and 1 µg g-1, respectively, while in beech wood approximately 100 and 20 µg g-1 were found, respectively. Sulfur in beech was dominated by sulfate-esters. In contrast, pine wood also contained larger amounts of reduced S. Three out of four selected fungi caused a reduction of the S oxidation state in wood from oxidized S (sulfate-ester, sulfate) to intermediate S (sulfonate, sulfoxide) or reduced S (thiols, e.g., proteins, peptides, enzyme cofactors). Only O. placenta shifted thiol to sulfonate. Growth experiments of these fungi on selective minimal media showed that in particular cysteine (thiol), sulfonates, and sulfate enhanced total mycelium growth. Consequently, wood-degrading fungi were able to utilize a large variety of different wood S sources for growth but preferentially transformed in vivo sulfate-esters and thiol into biomass structures. KW - Basidiomycetes KW - Fungi KW - S K-edge X-ray absorption near edge spectroscopy (XANES) KW - Sulfur oxidation status KW - Sulfate-esters PY - 2011 U6 - https://doi.org/10.1016/j.ibiod.2011.08.013 SN - 0964-8305 VL - 65 IS - 8 SP - 1215 EP - 1223 PB - Elsevier CY - Barking AN - OPUS4-27546 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Dudek, Gabriele A1 - Pritzkow, Wolfgang A1 - Riebe, Gundel A1 - Görner, Wolf A1 - Alber, D. T1 - Characterization of neutron transmuted zinc traces in pure copper materials by isotope dilution mass spectrometry N2 - The neutron transmutation doping (NTD) of highly pure copper with zinc was investigated as a promising means of achieving controlled gradation of the zinc content in the range 1-20 wg g-1. The doping process leads to the enrichment of two stable isotopes 64Zn and 66Zn in a ratio which differs from that of natural isotopic distribution. Mass spectrometric investigations by thermal ionization mass spectrometry (TIMS) were performed to validate the results obtained by gamma spectrometry. The investigations included both determination of the isotopic ratios of the doped zinc isotopes and the analysis of the accumulated zinc contents by isotope dilution (ID) analysis. Thereby a sample-specific correction of the blank could be performed because the isotope 68Zn was not influenced, because of the transmutation process. The results obtained by TIMS prove the strict proportionality of the doped zinc content, in the range 5 to 20 wg g-1, to the neutron fluence. Comparison with gamma spectrometric results showed a very good agreement within the uncertainties. PY - 2001 U6 - https://doi.org/10.1007/s002160100784 SN - 0937-0633 N1 - Geburtsname von Dudek, Gabriele: Wermann, G. - Birth name of Dudek, Gabriele: Wermann, G. VL - 370 IS - 5 SP - 606 EP - 611 PB - Springer CY - Berlin AN - OPUS4-1145 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Klingbeil, Patrick A1 - Vogl, Jochen A1 - Pritzkow, Wolfgang A1 - Riebe, Gundel A1 - Müller, J. T1 - Comparative studies on the certification of reference materials by ICPMS and TIMS using isotope dilution procedures N2 - A comparison of different isotope dilution mass spectrometric (IDMS) procedures using inductively coupled plasma mass spectrometry (ICPMS) and thermal ionization mass spectrometry (TIMS) was carried out to examine the degree of equivalence between the used procedures in terms of requirements for reference material certification. The comparison was based on the measurement results and their uncertainties. The sample used in this study is a pure zinc metal to be certified by the Bureau Communie de Référence (BCR) for amount contents of different trace elements. This study focuses on cadmium and thallium. The TIMS values contributed to the certified values. To guarantee identical conditions as far as possible for the procedures under investigation, the samples were split into subsamples after spiking and digestion took place. Thus, every IDMS procedure started with an identical set of samples. In total, four different IDMS procedures and one external calibration procedure using internal standardization as an example of routine analysis were applied. The IDMS procedures divide in a group with and a group without trace/matrix separation. Multicollector TIMS (TI-MC-MS) and multicollector ICPMS (ICP-MC-MS) were used in combination with trace/matrix separation, whereas quadrupole ICPMS (ICP-QMS) and ICP-MC-MS were also applied to nonseparated samples. All IDMS results agree well within their combined uncertainties, while some results from the external calibration procedure do not. IDMS results obtained by ICPMS without separation are comparable to those obtained by TI-MC-MS with separation regarding precision and accuracy. The smallest uncertainties were achieved using ICP-MC-MS in combination with trace/matrix separation. KW - ICP-MS KW - TIMS KW - IDMS KW - multi-collector KW - uncertainty budget KW - Certification KW - reference material PY - 2001 U6 - https://doi.org/10.1021/ac001278c SN - 0003-2700 SN - 1520-6882 VL - 73 IS - 8 SP - 1881 EP - 1888 PB - American Chemical Society CY - Washington, DC AN - OPUS4-909 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Bohn, T. A1 - Walczyk, Th. A1 - Davidsson, L. A1 - Pritzkow, Wolfgang A1 - Klingbeil, Patrick A1 - Vogl, Jochen A1 - Hurrell, R.F. T1 - Comparison of urinary monitoring, faecal monitoring and erythrocyte analysis of stable isotope labels to determine magnesium absorption in human subjects KW - Magnesium absorption KW - Stable isotopes KW - Faecal monitoring KW - Urinary monitoring KW - Erythrocytes PY - 2004 SN - 0007-1145 SN - 1475-2662 VL - 58 SP - 113 EP - 120 PB - CABI Publ. CY - Wallingford AN - OPUS4-3362 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Pritzkow, Wolfgang A1 - Vogl, Jochen A1 - Berger, Achim A1 - Ecker, Klaus A1 - Grötzschel, R. A1 - Klingbeil, Patrick A1 - Persson, L. A1 - Riebe, Gundel A1 - Wätjen, U. T1 - Contribution of ICP-IDMS to the certification of antimony implanted in a silicon wafer - comparison with RBS and INAA results N2 - A thin-layer reference material for surface and near-surface analytical methods was produced and certified. The surface density of the implanted Sb layer was determined by Rutherford backscattering spectrometry (RBS), instrumental neutron activation analysis (INAA), and inductively coupled plasma isotope dilution mass spectrometry (ICP-IDMS) equipped with a multi-collector. The isotopic abundances of Sb (121Sb and 123Sb) were determined by multi-collector ICP-MS and INAA. ICP-IDMS measurements are discussed in detail in this paper. All methods produced values traceable to the SI and are accompanied by a complete uncertainty budget. The homogeneity of the material was measured with RBS. From these measurements the standard uncertainty due to possible inhomogeneities was estimated to be less than 0.78% for fractions of the area increments down to 0.75 mm2 in size. Excellent agreement between the results of the three different methods was found. For the surface density of implanted Sb atoms the unweighted mean value of the means of four data sets is 4.8121016 cm-2 with an expanded uncertainty (coverage factor k=2) of 0.0921016 cm-2. For the isotope amount ratio R (121Sb/123Sb) the unweighted mean value of the means of two data sets is 1.435 with an expanded uncertainty (coverage factor k=2) of 0.006. KW - ICP-MS KW - IDMS KW - Reference material KW - Surface analysis PY - 2001 U6 - https://doi.org/10.1007/s002160100987 SN - 0937-0633 VL - 371 SP - 867 EP - 873 PB - Springer CY - Berlin AN - OPUS4-7220 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Pritzkow, Wolfgang A1 - Vogl, Jochen A1 - Köppen, Robert A1 - Ostermann, Markus T1 - Determination of sulfur isotope abundance ratios for SI-traceable low sulfur concentration measurements in fossil fuels by ID-TIMS N2 - The present work describes the development of an existing TIMS procedure to a reference procedure for low sulfur concentration measurements in fossil fuels. With this enhanced procedure SI-traceable sulfur mass fractions below 10 mg kg-1 can be obtained. The achieved detection limit is approximately 0.2 mg kg-1. The procedure was validated by certified reference materials. The procedure was already applied to candidate reference materials and to samples analysed within projects of the “Comité Consultatif pour la Quantité de Matière – CCQM”. Additionally the influence of the isotopic composition on the results and its corresponding uncertainty was studied. Reference data published in the literature on the isotopic composition of sulfur were assessed. KW - TIMS KW - IDMS KW - Low sulfur KW - Fossil fuel KW - Reference procedure PY - 2005 U6 - https://doi.org/10.1016/j.ijms.2004.10.024 SN - 0168-1176 SN - 0020-7381 SN - 1387-3806 SN - 1873-2798 VL - 242 SP - 309 EP - 318 PB - Elsevier CY - Amsterdam AN - OPUS4-7222 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Dudek, Gabriele A1 - Alber, D. A1 - Pritzkow, Wolfgang A1 - Riebe, Gundel A1 - Vogl, Jochen A1 - Görner, Wolf T1 - Determination of the beta- branching ratio of 64Cu by mass spectrometric investigations of the decay products in neutron transmuted copper N2 - The ?- branching ratio of 64Cu was determined by investigating the resulting decay products in copper doped by neutron transmutation. The numbers of 64Zn and 64Ni atoms were analyzed using isotope dilution analysis combined with thermal ionization mass spectrometry. A ?- branching ratio of (38.06±0.30)% was obtained, which agrees with the study of Kawada (Appl. Radiat. Isot. 37 (1) (1986) 7) to a higher accuracy. However, our result differs from the value cited in the NUDAT database of (39.0±0.3)%. KW - NTD KW - Copper KW - Zinc KW - Nickel KW - Branching ratio KW - Cu-64 KW - TIMS KW - IDMS KW - Isotope dilution KW - Thermal ionization mass spectrometry KW - Neutron transmutation doping PY - 2002 U6 - https://doi.org/10.1016/S0969-8043(01)00180-4 SN - 0883-2889 SN - 0969-8043 N1 - Geburtsname von Dudek, Gabriele: Wermann, G. - Birth name of Dudek, Gabriele: Wermann, G. VL - 56 SP - 145 EP - 151 PB - Elsevier CY - Amsterdam AN - OPUS4-7221 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -