TY - CONF A1 - Pauli, Jutta A1 - Jäger, Christian T1 - Materialanalytik mittels der Festkörper-NMR: Von Biokeramiken über Polymere bis zu Oberflächenschichten T2 - Abteilungsseminar, Abteilung I, BAM T2 - Abteilungsseminar, Abteilung I, BAM CY - Berlin, Germany DA - 2003-03-01 PY - 2003 AN - OPUS4-3748 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jäger, Christian A1 - Hartmann, P. A1 - Barth, S. A1 - Vogel, J. A1 - Pauli, Jutta A1 - Schmauder, H.-P. T1 - NMR und Biomaterialien - Was kann man über plasmagespritzte Apatitschichten und Cellulose lernen? T2 - Kolloquium des SFB 428 T2 - Kolloquium des SFB 428 CY - Freiburg, Deutschland DA - 2002-12-10 PY - 2002 AN - OPUS4-2127 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Reinsch, Stefan A1 - Dreßler, Martin A1 - Nofz, Marianne A1 - Pauli, Jutta A1 - Jäger, Christian A1 - Scholz, G. ED - Krämer, V. T1 - Phasentransformationen von anorganisch modifizierten Alumo-Xerogelen T2 - Jahrestagung der Gesellschaft für Thermische Analyse T2 - Jahrestagung der Gesellschaft für Thermische Analyse e.V. CY - Augsburg, Deutschland DA - 2003-09-15 PY - 2003 SP - 1 EP - 2 AN - OPUS4-2668 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jäger, Christian A1 - Pauli, Jutta A1 - Berger, Georg A1 - Spitzer, Andrea T1 - Entwicklung neuartiger Knochenersatzwerkstoffe und strukturelle Charakterisierung mittels der 31P-NMR-Spektroskopie T2 - Tagungsband "Tag der Chemie" 2002 T2 - Tagung "Tag der Chemie" CY - Berlin, Deutschland DA - 2002-12-04 PY - 2002 AN - OPUS4-1900 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Peuker, Christel A1 - Reinholz, Uwe A1 - Jäger, Christian A1 - Pauli, Jutta A1 - Geißler, Heinz T1 - Extinction coefficients of the OH bands in the IR spectra of basic and water-enriched silicate and aluminosilicate glasses JF - Glass science and technology PY - 2003 SN - 0927-4472 VL - 76 IS - 5 SP - 227 EP - 233 PB - Elsevier CY - Amsterdam AN - OPUS4-2781 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Vag, T. A1 - Resch-Genger, Ute A1 - Haag, R. A1 - Werner, A. A1 - Hilger, I. T1 - Evaluierung neuer Fluoreszenzfarbstoffe für die in-vivo-Diagnostik T2 - 88. Deutscher Röntgenkongress T2 - 88. Deutscher Röntgenkongress CY - Berlin, Germany DA - 2007-05-16 PY - 2007 AN - OPUS4-13877 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Spieles, Monika A1 - Resch-Genger, Ute A1 - Licha, K. T1 - Analytical and Spctroscopic tools for the Design of Highly Flourescent Conjugates T2 - ANAKON 2013 T2 - ANAKON 2013 CY - Essen, Germany DA - 2013-03-04 PY - 2013 AN - OPUS4-28471 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Brehm, Robert A1 - Grabolle, Markus A1 - Behnke, Thomas A1 - Mathejczyk, J. A1 - Hamann, F. A1 - Alves, F. A1 - Hilger, I. A1 - Resch-Genger, Ute ED - Achilefu, S. ED - Raghavachari, R. T1 - Dye-biomolecule conjugates and NIR-fluorescent particles for targeting of disease-related biomarkers T2 - Proceedings of SPIE - Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications III N2 - Indispensable for fluorescence imaging are highly specific and sensitive molecular probes that absorb and emit in the near infrared (NIR) spectral region and respond to or target molecular species or processes. Here, we present approaches to targeted fluorescent probes for in vivo imaging in the intensity and lifetime domain exploiting NIR dyes. Screening schemes for the fast identification of suitable fluorophores are derived and design criteria for highly emissive optical probes. In addition, as a signal amplification strategy that enables also the use of hydrophobic NIR fluorophores as fluorescent reporters, first steps towards versatile strategies for the preparation of NIR-fluorescent polymeric particles are presented that can be utilized also for the design of targeted and analyte-responsive probes. KW - Fluorescence KW - Fluorescence lifetime imaging KW - Near-infrared KW - NIR KW - Cyanine dye KW - Cancer KW - In vivo imaging KW - Aggregation KW - Nanoparticle PY - 2011 DO - https://doi.org/10.1117/12.876828 SN - 1605-7422 N1 - Serientitel: Proceedings of SPIE – Series title: Proceedings of SPIE IS - 7910 SP - 791014-1 EP - 791014-15 AN - OPUS4-24353 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Jäger, Christian A1 - Kraus, Werner A1 - Reck, Günter A1 - Berger, Georg T1 - Ambiguities of comparative XRD and NMR studies of crystalline calcium phosphates T2 - 25. Jahrestagung der Fachgruppe "Magnetische Resonanzspektroskopie" T2 - 25. Jahrestagung der Fachgruppe "Magnetische Resonanzspektroskopie" CY - Leipzig, Germany DA - 2003-09-30 PY - 2003 AN - OPUS4-4736 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Resch-Genger, Ute A1 - Lisy, M.-R. A1 - Hilger, I. A1 - Kaiser, W. T1 - Influence of biological systems on the intensity of the light emission of fluorophores T2 - Molekulare Bildgebung T2 - Molekulare Bildgebung CY - Kiel, Germany DA - 2007-07-05 PY - 2007 AN - OPUS4-16404 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Resch-Genger, Ute A1 - Vag, T. A1 - Haag, R. A1 - Hilger, I. A1 - Kaiser, W. T1 - Spectroscopic properties of new near infrared dyes for molecular imaging T2 - Molekulare Bildgebung T2 - Molekulare Bildgebung CY - Kiel, Germany DA - 2007-07-05 PY - 2007 AN - OPUS4-16405 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Resch-Genger, Ute A1 - Lisy, M.-R. A1 - Thomas, C. A1 - Kaiser, W. A1 - Hilger, I. T1 - Design and characterization of a new contrast agent for the specific and sensitive detection of tumor via in vivo fluorescence imaging T2 - Method and Application of Fluerescence (MAF10) T2 - Method and Application of Fluerescence (MAF10) CY - Salzburg, Austria DA - 2007-09-09 PY - 2007 AN - OPUS4-16406 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Resch-Genger, Ute A1 - Vag, T. A1 - Haag, R. A1 - Kaiser, W. A1 - Hilger, I. T1 - Characterization of a new near infrared dyes for molecular imaging T2 - Method and Application of Fluerescence (MAF10) T2 - Method and Application of Fluerescence (MAF10) CY - Salzburg, Austria DA - 2007-09-09 PY - 2007 AN - OPUS4-16407 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Peplinski, Burkhard A1 - Berger, Georg T1 - NMR- und XRD-Charakterisierung von Tricalciumphosphat und alkalihaltigen Calciumphosphaten T2 - AK Biokeramik der DKG/DGM, BAM T2 - AK Biokeramik der DKG/DGM, BAM CY - Berlin, Germany DA - 2005-04-27 PY - 2005 AN - OPUS4-7369 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Grabolle, Markus A1 - Resch-Genger, Ute A1 - Licha, K. A1 - Welker, P. T1 - Sharpening the fluorescence properties of cyanine bioconjugates: a novel series of highly sulfonated pentamethine cyanines T2 - ESMI 2013 T2 - ESMI 2013 CY - Turin, Italy DA - 2013-05-27 PY - 2013 AN - OPUS4-28955 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Napp, J. A1 - Streng, Julia A1 - Alves, F. A1 - Resch-Genger, Ute T1 - Brilliant and Functional Herceptin Conjugates with a Remarkably High Number of Bound Fluorophores T2 - MOBI 2013 T2 - MOBI 2013 CY - Heidelberg, Germany DA - 2013-10-28 PY - 2013 AN - OPUS4-29576 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Spieles, Monika A1 - Licha, K. A1 - Resch-Genger, Ute T1 - Design of Highly Fluorescent Conjugates T2 - MOBI 2013 T2 - MOBI 2013 CY - Heidelberg, Germany DA - 2013-10-28 PY - 2013 AN - OPUS4-29577 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Grabolle, Markus A1 - Resch-Genger, Ute A1 - Licha, K. A1 - Berkemeyer, J. T1 - Suitable Labels for Molecular Imaging - Influence of Dye Hydrophilicity on the Spectroscopic Properties of lgG Conjugates T2 - 23rd Lecture Conference on Photochemistry T2 - 23rd Lecture Conference on Photochemistry CY - Potsdam, Germany DA - 2012-10-08 PY - 2012 AN - OPUS4-26967 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Würth, Christian A1 - Pauli, Jutta A1 - Weigert, Florian T1 - Quantitative optical-spectroscopic characterization of luminescent nanomaterials - Photoluminescence Quantum Yields N2 - Accurate and quantitative photoluminescence measurements are mandatory for the comparison of different emitter classes and the rational design of the next generation of molecular and nanoscale reporters as well as for most applications relying on their luminescence features in the life and material sciences and nanobiophotonics. In the following, procedures for the determination of the spectroscopic key parameter photoluminescence quantum yield, i.e., the number of emitted per absorbed photons, in the UV/vis/NIR/SWIR are presented including pitfalls and achievable uncertainties and material-specific effects related to certain emitter classes are addressed. Special emphasis is dedicated to luminescent nanocrystals. T2 - International Workshop on "Emerging Nanomaterials for Displays and SSL" CY - Dresden, Germany DA - 11.11.2021 KW - Nano KW - Nanomaterial KW - Nanocrystal KW - Semiconductor quantum dot KW - Lanthanide KW - Photoluminescence KW - Quantum yield KW - Photophysics KW - Lifetime KW - Surface chemistry KW - Brightness KW - NIR KW - SWIR KW - Method KW - Uncertainty KW - Rreference material PY - 2021 AN - OPUS4-53783 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Würth, Christian A1 - Güttler, Arne A1 - Resch-Genger, Ute T1 - Reliable Determination of the Signal-Relevant Spectroscopic Key Characteristics of Luminescent Reporters and Optical Probes for Imaging in the vis/NIR/SWIR N2 - Introduction. Comparing different emitter classes and rationally designing the next generation of molecular and nanoscale probes for bioimaging applications require accurate and quantitative methods for the measurement of the key parameter photoluminescence quantum yield f.1 f equals the number of emitted per number of absorbed photons. This is particularly relevant for increasingly used fluorescence imaging in the short wave-infrared region (SWIR) ≥ 900 nm providing deeper penetration depths, a better image resolution, and an improved signal-to-noise or tumor-to-background ratio.2, 3 However, spectroscopic measurements in the SWIR are more challenging and require specific calibrations and standards. T2 - EMIM 2021 CY - Göttingen, Germany DA - 24.08.2021 KW - Fluorescence KW - Optical probe KW - Dye KW - Photophysics KW - Quantum yield KW - Mechanism KW - NIR KW - SWIR KW - Imaging KW - Reference material KW - Reliability KW - Nano KW - Particle KW - Method KW - Quality assurance PY - 2021 AN - OPUS4-53233 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Napp, J. A1 - Pochstein, Marieke A1 - Streng, Julia A1 - Alves, F. A1 - Resch-Genger, Ute T1 - Bright Functional Herceptin Conjugates with a Remarkably High Number of Fluorophores for Optical Imaging T2 - 5th EuCheMS T2 - 5th EuCheMS CY - Istanbul, Turkey DA - 2014-08-31 PY - 2014 AN - OPUS4-31849 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CHAP A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - Pauli, Jutta ED - Brahme, A. T1 - Signal-relevant properties of fluorescent labels and optical probes and their determination T2 - Comprehensive biomedical physics PY - 2014 SN - 978-0-444-53632-7 DO - https://doi.org/10.1016//B978-0-444-53632-7.00408-1 VL - 4 SP - Chapter 4.02, 15 EP - 26 PB - Elsevier CY - Amsterdam AN - OPUS4-31433 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Grabolle, Markus A1 - Brehm, Robert A1 - Resch-Genger, Ute A1 - Hamann, F. A1 - Hilger, A. T1 - Influence of the Dimerization of Fluorescent Dyes on the Spectroscopic Properties of their lgG Conjugates T2 - Molekulare Bildgebung 10 (MOBI10) T2 - Molekulare Bildgebung 10 (MOBI10) CY - Seeon-Seebruck, Germany DA - 2010-11-04 PY - 2010 AN - OPUS4-22661 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Behnke, Thomas A1 - Brehm, Robert A1 - Grabolle, Markus A1 - Hennig, Andreas A1 - Hoffmann, Angelika A1 - Hoffmann, Katrin A1 - Linck, Lena A1 - Lochmann, Cornelia A1 - Pauli, Jutta A1 - Spieles, Monika A1 - Würth, Christian T1 - Funktionelle Chromophor-Systeme, innovative Validierungskonzepte und rückführbare Standards für die fluoreszenzbasierte multiparametrische Bioanalytik T2 - 5. Senftenberger Innovationsforum Multiparameteranalytik (Proceedings) N2 - Unter dem Motto „Innovation und Qualitätssicherung in der (Bio)Analytik“ werden in der Arbeitsgruppe Fluoreszenzspektroskopie der BAM, Bundesanstalt für Materialforschung und -prüfung, funktionelle Chromophor-Systeme, einfache Signalverstärkungs- und Multiplexingstrategien sowie innovative Validierungs- und rückführbare Standardisierungskonzepte für verschiedene fluorometrische Messgrößen und Methoden entwickelt. Im Mittelpunkt stehen dabei molekulare Fluorophore, Nanokristalle mit größenabhängigen optischen Eigenschaften (sogenannte Quantenpunkte, QDs) und fluoreszierende Partikel variabler Größe sowie Sonden und Sensormoleküle für neutrale und ionische Analyte und für die Charakterisierung von funktionellen Gruppen. Dabei erfolgen auch methodische Entwicklungen für die Fluoreszenzspektroskopie, die Fluoreszenzmikroskopie, die Milcrofluorometrie, die Sensorik und die Mikroarraytechnologie. Ziele sind u. a. das Design und die Untersuchung von multiplexfähigen selektiven und sensitiven Sonden für die Biomarkeranalytik, die Entwicklung von Methoden zur Charakterisierung der signalrelevanten Eigenschaften dieser Chromophor-Systeme und zur Charakterisierung von funktionellen Gruppen an Oberflächen und ihre Validierung sowie die Entwicklung und Bereitstellung von formatadaptierbaren, flexibel ersetzbaren Standards für die fluoreszenzbasierte Multiparameteranalytik. T2 - 5. Senftenberger Innovationsforum Multiparameteranalytik CY - Senftenberg, Deutschland DA - 10.03.2011 KW - Multiparametric KW - Multiplexing KW - Fluorescence KW - Nanoparticles KW - NIR dyes KW - Surface analysis KW - Quantum yield KW - Quantum dot KW - Lifetime PY - 2011 SP - 86 EP - 108 CY - Senftenberg AN - OPUS4-23635 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Pauli, Jutta A1 - Wycisk, V. A1 - Welker, P. A1 - Justies, A. A1 - Resch-Genger, Ute A1 - Haag, R. A1 - Licha, K. T1 - Glycerol-Based Contrast Agents: A Novel Series of Dendronized Pentamethine Dyes JF - Bioconjugate Chemistry N2 - The synthesis of water-soluble dyes, which absorb and emit in the range between 650 and 950 nm and display high extinction coefficients (ε) as well as high fluorescence quantum yields (Φf), is still a demand for optical imaging. We now present a synthetic route for the preparation of a new group of glycerol-substituted cyanine dyes from dendronized indole precursors that have been functionalized as Nhydroxysuccinimide (NHS) esters. High Φf values of up to 0.15 and extinction coefficients of up to 189 000 L mol−1 cm−1 were obtained for the pure dyes. Furthermore, conjugates of the new dendronized dyes with the antibody cetuximab (ctx) that were directed against the epidermal growth factor receptor (EGFR) of tumor cells could be prepared with dye to protein ratios between 0.3 and 2.2 to assess their potential as imaging probes. For the first time, ctx conjugates could be achieved without showing a decrease in Φf and with an increasing labeling degree that exceeded the value of the pure dye even at a labeling degree above 2. The incorporation of hydrophilically and sterically demanding dendrimers into cyanines prevented dimer formation after covalent conjugation to the antibody. The binding functionality of the resulting ctx conjugates to the EGFR was successfully demonstrated by cell microscopy studies using EGFR expressing cell lines. In summary, the combination of hydrophilic glycerol dendrons with reactive dye labels has been established for the first time and is a promising Approach toward more powerful fluorescent labels with less dimerization. KW - Dye KW - Quantum yield KW - Fluorescence KW - Contrast agent KW - Extinction coefficient PY - 2015 DO - https://doi.org/10.1021/acs.bioconjchem.5b00097 SN - 1043-1802 VL - 26 SP - 773 EP - 781 PB - ACS Publications CY - Washington AN - OPUS4-38880 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Spieles, Monika A1 - Schneider, Thomas A1 - Binder, Denny A1 - Güttler, Arne A1 - Resch-Genger, Ute T1 - Entwicklung von Fluoreszenzquantenausbeutestandards N2 - Lumineszenzmethoden und funktionelle Chromophore erlangten in den letzten Jahren breite Anwendungen in den Material- und Lebenswissenschaften wie z.B. in der medizinischen Diagnostik, der Beleuchtungs- und Displaytechnologie, der Photovoltaik oder im Sicherheitsbereich für die Echtheitskodierung. Entscheidend für eine hohe Effizienz und Nachweisempfindlichkeit funktioneller Chromophore ist eine große Helligkeit bzw. Brillanz. Brillante Stoffe zeichnen sich durch eine hohe Absorption des anregenden Lichtes (messbar über den molaren Extinktionskoeffizienten) aus sowie durch eine effiziente Umwandlung des absorbierten Lichtes in Lumineszenzlicht bzw. durch eine große Photolumineszenzquantenausbeute aus. Die Bestimmung der Schlüsselgröße Fluoreszenzquantenausbeute erfolgt i.a. relativ zu einem Farbstoff bekannter Fluoreszenzquantenausbeute über einen Vergleich der absorptionsgewichteten integralen Fluoreszenzspektren von Probe und Standard. Hierfür werden Referenzmaterialien mit zertifizierten Fluoreszenzquantenausbeuten benötigt, die bislang nicht verfügbar sind. Im folgenden werden die laufenden Forschungsarbeiten an einer Serie an Fluoreszenzquantenausbeutestandard vorgestellt, die im Spektralbereich von 350 nm – 1050 nm emittieren. Diese Materialien können auch zur Überprüfung von Ulbrichtkugelmessplätzen eingesetzt werden. T2 - Colloquium Optical Spectrometry (COSP) CY - Berlin, Germany DA - 27.11.2017 KW - Fluoreszenz KW - Referenzmaterial KW - Quantenausbeute PY - 2017 AN - OPUS4-44095 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Würth, Christian A1 - Güttler, Arne A1 - Schneider, Thomas A1 - Resch-Genger, Ute T1 - Determination of the Uncertainty Budgets of the Fluorescence Quantum Yield Values of Certified Standards as New Optical Reference Materials N2 - Luminescence techniques are amongst the most commonly used analytical methods in the life and material sciences due to their high sensitivity and their nondestructive and multiparametric character. Photoluminescence signals are, however, affected by wavelength-, polarization- and time-dependent instrument specific effects. This hampers the comparability of fluorescence measurements and calls for simple tools for instrument characterization and the quantification of measured fluorescence intensities. Well characterized fluorescence standards for instrument calibration and performance validation (IPV) can be used also to reference fluorescence signals. Of special importance is the reliable and accurate determination of photoluminescence quantum yields (Ф f), that equals the number of emitted per absorbed photons and presents the key performance parameter for emitter efficiency and the comparison of different luminophores. The determination of Ф f is typically done with the aid of so-called quantum yield standards with well-known Ф f values. These standards can also be applied to evaluate integrating sphere setups, which are increasingly being used for absolute measurements of Ф f values. In this respect, division biophotonics of BAM has certificated a set of Ф f standards, which absorb and fluorescence in the wavelength range from 350 to 1100 nm. In the following, the route to Ф f standards with reliable and traceable Ф f values with a complete uncertainty budget will be presented. T2 - Eurachem-Workshop „Uncertainty from sampling and analysis for accredited laboratories CY - Berlin, Germany DA - 19.11.2019 KW - Uncertainty KW - Fluorescence KW - Quantum yield standard KW - Reference Material PY - 2019 AN - OPUS4-49661 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Güttler, Arne A1 - Schneider, Thomas A1 - Würth, Christian A1 - Resch-Genger, Ute T1 - Certified Fluorescence Quantum Yield Standards as New Optical Reference Materials N2 - Luminescence techniques are amongst the most commonly used analytical methods in life and material sciences due to their sensitivity and their nondestructive and multiparametric character. Photoluminescence signals are, however, affected by wavelength, polarization and time dependent instrument specific effects, and provide only relative intensities. This hampers the comparability of fluorescence measure-ments and calls for simple tools for instrument characterization and the quantification of measured fluorescence intensities. Well characterized fluorescence standards for instrument calibration and performance validation (IPV) can be used as references for fluorescence signals. Of special importance is the correct determination of photoluminescence quantum yields (QF) (number of emitted per absorbed photons) that provides a direct comparison of the fluorescence efficiency of emitters. Such well characterized standards have been successfully developed by BAM for the relative determination of f values of transparent solutions of molecular and nanoscale emitters in the wavelength range from 350 and 1100 nm and will be soon certified. These standards can also be used to evaluate integrating sphere setups, which are increasingly being used for absolute measurements of QF values. T2 - ANAKON 2019 CY - Münster, Germany DA - 25.03.2091 KW - Fluoreszenz KW - Referenzmaterial PY - 2019 AN - OPUS4-47795 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ramirez Caro, Alejandra A1 - Pauli, Jutta A1 - Mota, Berta A1 - Crasselt, Claudia A1 - Artemeva, Elena A1 - Schmidt, Wolfram A1 - Resch-Genger, Ute T1 - A Spectroscopic Study of the Superplasticizer Effect on Early Cement Hydration N2 - Organic/inorganic mixtures were prepared from ordinary Portland cement (OPC), water (w/c 0.22), a fluorescent dye in aqueous solution (stable at alkaline pH; BAM-I), and two different comb shape polycarboxylates (PCEs), i.e., high charge (PCE-HC) and low charge (PCE-LC), respectively. Rheological and calorimetric measurements were performed prior to optical studies in order to select PCE concentrations. Absorption and fluorescence spectroscopy of the system OPC + BAM-I (CBAM-I) revealed maxima of dye BAM-I located at 645 nm and 663 nm, respectively. In presence of PCE-HC and PCE-LC, these mixtures displayed a small red shift in reflectance and a faster decrease in intensity compared to studies with CBAM-I; however, only slight differences were observed between the different PCEs. With time, all systems exhibited a decrease in intensity of BAM-I in absorption/reflectance and emission. This could be caused by dye adsorption and possibly decomposition when in contact with cement particles or hydration products. T2 - The Sixth International Symposium on Nanotechnology in Construction (NICOM6) CY - Hong Kong, China DA - 02.12.2018 KW - Cement KW - Optical Spectroscopy KW - Dye KW - Hydration PY - 2018 SP - 1 EP - 9 AN - OPUS4-49378 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schmidt, Wolfram A1 - Mota Gassó, Berta A1 - Sturm, Heinz A1 - Pauli, Jutta ED - Greim, M. ED - Kusterle, W. ED - Teubert, O. T1 - Influence of effects on nano and micro scale on the rheological performance of cement paste, mortar and concrete T2 - Rheologische Messungen an Baustoffen N2 - Chemical admixtures like superplasticisers or stabilising agents are of ever increasing importance for modern concrete technology. They liberate the workability of concrete from its dependency on water content, and thus, open the gate towards innovative and future oriented concrete technologies such as self-compacting concrete. Today, admixture addition has become common practice in concrete technology, but the understanding of their highly complex mode of operation is extremely difficult and demands for understanding of processes within the range between nanometres and centimetres. Due to its complex time-dependent, multi-phase and multi-scale behaviour, flowable concrete systems are highly complicated and cannot be described comprehensively by simple models. It is therefore extremely challenging to identify the relevant parameters that predominantly control flow phenomena on different size scales, since these may occur on any scale between the nano scale (e.g. superplasticizer adsorption) and macro scale (e.g. grading of the aggregates). The present study discusses fundamental mechanisms at the interface between particle or hydrate surfaces and the fluid phase at a very early stage of concrete formation, and links these effects to macroscopic flow phenomena. Methods are discussed that appear promising interdisciplinary tools for enhancement of the understanding of the relevant interactions that are responsible for the macroscopic flow of flowable concrete. T2 - 25. Workshop und Kolloquium Rheologische Messsungen an Baustoffen CY - Regensburg, Germany DA - 02.03.2016 KW - Rheology KW - Cement KW - Concrete KW - Superplasticizer KW - Nano scale PY - 2016 SN - 978-3-7345-1313-8 SP - 294 EP - 307 PB - tredition CY - Hamburg AN - OPUS4-36862 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ramirez Caro, Alejandra A1 - Pauli, Jutta A1 - Schmidt, Wolfram A1 - Resch-Genger, Ute T1 - Study of the superplasticizer-cement hydration interaction by optical spectroscopy N2 - Chemical admixtures like superplasticizers or stabilizing agents are of ever increasing importance for modern concrete technology. Although such admixtures have meanwhile become common practice in many applications of concrete technology, the understanding of these highly complex systems is still limited and the relevant parameters, which predominantly control the interaction between the superplasticizer and the cement components, have not been identified yet. Optical methods have been successfully used for the analysis and monitoring of the interactions between a broad variety of nanoscale and molecular systems like nanoparticles of various chemical composition and different types of organic ligands or biomolecules. This encouraged to assess the potential of these methods for the investigation of the interactions that occur at the interface between particle or hydrate surfaces and the fluid phase at a very early stage of concrete formation and to differentiate between different superplasticizer. T2 - 2. ICCCM CY - Munich, Germany DA - 10.10.2016 KW - Optical spectroscopy KW - Cement hydration PY - 2016 AN - OPUS4-38474 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schmidt, Wolfram A1 - Weba, Luciana A1 - Silbernagl, Dorothee A1 - Mota Gassó, Berta A1 - Höhne, Patrick A1 - Sturm, Heinz A1 - Pauli, Jutta A1 - Resch-Genger, Ute A1 - Steinborn, Gabriele ED - Khayat, Kamal Henry T1 - Influences of nano effects on the flow phenomena of self-compacting concrete T2 - Flowing toward Sustainability N2 - Chemical admixtures like superplasticisers or stabilising agents are of ever increasing importance for modern concrete technology. They liberate the workability of concrete from its dependency on water content, and thus, open the gate towards innovative and future oriented concrete technologies such as selfcompacting concrete. Meanwhile admixtures have become common practice in concrete technology, but the understanding of these highly complex polymers in the entire concrete system lags far behind their application. Due to its complex time-dependent, multi-phase and multi-scale behaviour, flowable concrete systems are highly complicated and cannot be described comprehensively by simple models. It is therefore extremely challenging to identify the relevant parameters that predominantly control flow phenomena on different size scales, since these may occur on any scale between the nano scale (e.g. superplasticizer adsorption) and macro scale (e.g. grading of the aggregates). The present study discusses fundamental mechanisms at the interface between particle or hydrate surfaces and the fluid phase at a very early stage of concrete formation, and links these effects to macroscopic flow phenomena. Methods are discussed that appear promising interdisciplinary tools for enhancement of the understanding of the relevant interactions that are responsible for the macroscopic flow of flowable concrete. T2 - 8th International RILEM Symposium on Self-Compacting Concrete CY - Washington, D.C., USA DA - 15.05.2016 KW - Adsorption KW - Analytics KW - Hydration KW - Polycarboxylate ether KW - Rheology PY - 2016 SP - 245 EP - 254 AN - OPUS4-36882 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ramirez Caro, Alejandra A1 - Mota, Berta A1 - Artemeva, E. A1 - Pauli, Jutta A1 - Schmidt, Wolfram A1 - Resch-Genger, Ute T1 - A spectroscopic study of the superplasticizer effect on early cement hydration T2 - Tagungsband der 20. Internationalen Baustofftagung ibausil N2 - Organic/inorganic mixtures were prepared from ordinary Portland cement (OPC), water (w/c 0.22), a fluorescent dye in aqueous solution (stable at alkaline pH; BAM-I), and two different comb shape polycarboxylates (PCEs), i.e., high charge (PCE-HC) and low charge (PCE-LC), respectively. Rheological and calorimetric measurements were performed prior to optical studies in order to select PCE concentrations. Absorption and fluorescence spectroscopy of the system OPC + BAM-I (CBAM-I) revealed maxima of dye BAM-I located at 645 nm and 663 nm, respectively. In presence of PCE-HC and PCE-LC, these mixtures displayed a small red shift in reflectance and a faster decrease in intensity compared to studies with CBAM-I; however, only slight differences were observed between the different PCEs. With time, all systems exhibited a decrease in intensity of BAM-I in absorption/reflectance and emission. This could be caused by dye adsorption and possibly decomposition when in contact with cement particles or hydration products. T2 - 20. Internationale Baustofftagung CY - Weimar, Germany DA - 12.09.2018 KW - Optical spectroscopie KW - Cement hydration KW - Dyes PY - 2018 SN - 978-3-00-059950-7 VL - 20 SP - 1 EP - 6 PB - F.A. Finger-Institut für Baustoffkunde CY - Weimar AN - OPUS4-46277 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Mota Gassó, Berta A1 - Schmidt, Wolfram A1 - Pauli, Jutta A1 - Sturm, Heinz T1 - Influences of hydration effects on the flow phenomena of concrete with admixtures T2 - GDCh-Monograph N2 - Today, chemical admixtures like superplasticisers and stabilising agents are extremely important for modern concrete technology. These agents have meanwhile become common practice in concrete technology, but the understanding within the entire system lags far behind their application. The macroscopic rheology of concrete in the presence of superplasticizers strongly depends upon effects on a much smaller scale such as the hydration of the cement, the adsorption of superplasticizers, and the pore solution chemistry. T2 - 2nd International Conference on the Chemistry of Construction Materials CY - München, Germany DA - 10.10.2016 KW - Rheology KW - Cement KW - Superplasticiser PY - 2016 SN - 978-3-936028-96-6 VL - 50 SP - 276 EP - 279 PB - Gesellschaft Deutscher Chemiker e.V. CY - Frankfurt am Main AN - OPUS4-38849 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ramirez Caro, Alejandra A1 - Pauli, Jutta A1 - Schmidt, Wolfram A1 - Resch-Genger, Ute T1 - Study of the superplasticizer-cement hydration interaction by optical spectroscopy T2 - GDCh-Monograph N2 - Nowadays, superplasticizers (SPs) are widely used to increase fluidity and reduce water content in concrete; thus, allowing better workability for final applications. The present study will focus on the hydration effect using comb shape polycarboxylates (PCEs), which are known to allow a very low water/cement ratio (w/c of 0.20) or less.Optical methods have been successfully used for the analysis and monitoring of the interactions between a broad variety of nanoscale and molecular systems like nanoparticles of various chemical composition and different types of organic ligands or biomolecules.This encouraged us to assess the potential of these methods, and particularly fluorescence, for the investigation of the interactions that occur at the interface between hydrate surfaces of cement particles and PCE at a very early stage of concrete formation and to differentiate between the impact of PCE’s molecular structures on such interactions. T2 - 2nd International Conference on the Chemistry of Construction Materials CY - Munich, Germany DA - 10.10.2016 KW - Cement KW - Dye KW - Superplasticizers KW - Fluorescence PY - 2016 SN - 978-3-936028-96-6 VL - 50 SP - 260 EP - 263 PB - Gesellschaft Deutscher Chemiker e.V. CY - Frankfurth am Main AN - OPUS4-38881 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ramirez Caro, Alejandra A1 - Pauli, Jutta A1 - Artemeva, Elena A1 - Ermilov, Eugeny A1 - Crasselt, Claudia A1 - Stroh, Julia A1 - Mota Gasso, Berta A1 - Schmidt, Wolfram A1 - Resch-Genger, Ute T1 - Study of the hydration of superplasticizer-cement pastes with optical spectroscopy N2 - Chemical admixtures like superplasticizers or stabilizing agents are of ever increasing importance for modern concrete technology. Although such admixtures have meanwhile become common practice in many applications of concrete technology, the understanding of these highly complex systems is still limited and the relevant parameters, which predominantly control the interaction between the superplasticizer and the cement components, have not been identified yet. Optical methods have been successfully used for the analysis and monitoring of the interactions between a broad variety of nanoscale and molecular systems like nanoparticles of various chemical composition and different types of organic ligands or biomolecules. This encouraged us to assess the potential of these methods, and particularly reflectance and fluorescence measurements, for the study of the interactions that occur at the interface between particle or hydrate surfaces and the fluid phase at a very early stage of concrete formation. Special emphasis is dedicated to search for and identify differences between commonly used superplasticizers. Here, we focus on hydration effects using commercial comb shape polycarboxylate ethers (PCEs) with different charge densities, which are known to allow a very low water/cement ratio (w/c of 0.20 or less) while maintaining good workability. Based upon changes of the intensity of the reflectance and fluorescence signal and spectral effects of a dye, acting as optical reporter, a model for the interactions of dye, PCE molecules and cement nanoparticles in the very first phase of cement hydration is derived T2 - Gesellschaft Deutscher Chemiker-Analytische Chemie-Anakon 2017 CY - Tübingen, Germany DA - 03.04.2017 KW - Cement hydration KW - Optical spectroscopy KW - Superplasticizers PY - 2017 AN - OPUS4-39882 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -