TY - CONF A1 - Meyer, Susann A1 - Schröter, Astrid-Maria A1 - Hahn, Marc Benjamin A1 - Solomun, Tihomir A1 - Sturm, Heinz A1 - Kunte, Hans-Jörg T1 - Structural changes in plasmid DNA verified by gel electrophoresis and AFM: Sample radiation and damage detection N2 - Many microorganisms possess circular molecules of plasmid DNA. Their conformation may vary from more compact to relaxed forms. Structural changes have important consequences for the physiological role of plasmids and will influence plasmid transcription, replication and their susceptibility to environmental stresses. Radiation is a common form of stress and can cause structural damage to DNA such as single (SSB) and double strand breaks (DSB). Recently, it has been indicated that secondary low-energy electrons play an important role in biological damage under vacuum condition. Therefore, our studies focus on irradiating the plasmid pUC19 with low-energy electrons for the first time under physiological conditions. T2 - AFM BioMed Conference CY - Porto, Portugal DA - 11.04.2016 KW - Irradiation damage KW - Low energy electrons KW - DNA PY - 2016 AN - OPUS4-35835 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -