TY - JOUR A1 - Garrido, E. A1 - Climent Terol, Estela A1 - Marcos, M. D. A1 - Sancenón, F. A1 - Rurack, Knut A1 - Martínez-Máñez, R. T1 - Dualplex lateral flow assay for simultaneous scopolamine and "cannibal drug" detection based on receptor-gated mesoporous nanoparticles N2 - We report herein the design of a strip-based rapid test utilizing bioinspired hybrid nanomaterials for the in situ and at site detection of the drug scopolamine (SCP) using a smartphone for readout, allowing SCP identification in diluted saliva down to 40 nM in less than 15 min. For this purpose, we prepared a nanosensor based on mesoporous silica nanoparticles loaded with a fluorescent reporter (rhodamine B) and functionalized with bethanechol, a potent agonist of recombinant human muscarinic acetylcholine receptor M2 (M2-AChR). M2-AChR interaction with the anchored bethanechol derivative leads to capping of the pores. The sensing mechanism relies on binding of SCP to M2-AChR resulting in pore opening and delivery of the entrapped rhodamine B reporter. Moreover, the material was incorporated into strips for lateral-flow assays coupled to smartphone readout, giving fast response time, good selectivity, and exceptional sensitivity. In an attempt to a mobile analytical test system for law enforcement services, we have also developed a dualplex lateral flow assay for SCP and 3,4-methylenedioxypyrovalerone (MDPV) also known as the so-called “cannibal drug”. KW - Rapid tests KW - Vor-Ort-Analytik KW - Lateral Flow Assays KW - Scopolamine KW - Gesteuerte Freisetzung KW - Cannibal Drug PY - 2022 U6 - https://doi.org/10.1039/d2nr03325a SP - 1 EP - 9 PB - Royal Society of Chemistry CY - Cambridge AN - OPUS4-55744 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Garrido, E. A1 - Hernández-Sigüenza, G. A1 - Climent, Estela A1 - Marcos, M. D. A1 - Rurack, Knut A1 - Gaviña, P. A1 - Parra, M. A1 - Sancenón, F. A1 - Martí-Centelles, V. A1 - Martínez-Máñez, R. T1 - Strip-based lateral flow-type indicator displacement assay for γ-hydroxybutyric acid (GHB) detection in beverages N2 - The use of gamma-hydroxybutyric acid (GHB) in drug-facilitated sexual assault has increased due to its availability and high solubility in aqueous solutions and alcoholic beverages, necessitating the development of rapid methods for GHB detection. In this respect, portable testing methods for use in the field, based on lateral flow assays (LFAs) and capable of detecting trace concentrations of target analytes, are particularly attractive and hold enormous potential for the detection of illicit drugs. Using this strategy, here we report a rapid, low cost, easy-to-handle strip-based LFA for GHB analysis employing a smartphone for fluorescence readout. At molecular signalling level, the ensemble is based on a Cu2+ complex with a tetradentate ligand and the fluorescent dye coumarin 343, which indicate GHB through an indicator displacement assay (IDA) in aqueous solution. When incorporated in a LFA-based strip test this system shows a detection limit as low as 0.03 μM for GHB in MES buffer solution and is able to detect GHB at concentrations of 0.1 μM in soft drinks and alcoholic beverages in only 1 min. KW - Indicator displacement assay KW - Gamma-hydroxybutyric acid KW - Lateral flow assay KW - Test strip KW - Fluorescent dyes KW - Smartphone readout PY - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:b43-564631 SN - 0925-4005 VL - 377 SP - 1 EP - 7 PB - Elsevier B.V. CY - Amsterdam AN - OPUS4-56463 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -