TY - CONF A1 - Lehmann, Andreas T1 - Vergleich HPLC <-> CE T2 - HPLC-Grundkurs CY - Berlin, Deutschland DA - 2000-09-04 PY - 2000 PB - Wista CY - Berlin AN - OPUS4-910 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Walther, Wolfgang A1 - Rohde, Gabriele ED - Frenzel, W. ED - Linscheid, M. ED - Seubert, A. T1 - Einsatz der Kapillarelektrophorese für die Zertifizierung eines Referenzmaterials "Pentachlorphenol in Boden" T2 - 1. Konferenz über Ionenanalyse CY - Berlin, Deutschland DA - 2001-02-21 PY - 2001 CY - Berlin AN - OPUS4-1059 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas T1 - Aktuelle HPLC/MS-Kopplungen T2 - HPLC-Grundkurs CY - Berlin, Germany DA - 2000-09-04 PY - 2000 AN - OPUS4-3275 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Radig, W. A1 - Dimmig, Th. A1 - Lehmann, Andreas A1 - Krüger, Ralph-Peter A1 - Schäfer, V. T1 - Struktur des basischen Zink-O,O'-ethylhexyldithiophosphats in unpolar, aprotischen Lösungsmitteln PY - 1995 SN - 0941-1216 VL - 337 SP - 647 EP - 654 PB - Wiley-VCH Verl. CY - Weinheim AN - OPUS4-2145 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Walther, Wolfgang A1 - Becker, Roland A1 - Nehls, Irene A1 - Lehmann, Andreas A1 - Redlich, Christel A1 - Flemig, Sabine A1 - Dorgerloh, Ute A1 - Win, Tin A1 - Buge, Hans-Gerhard T1 - Pentchlorophenol (PCP) in Soil - Certified Reference Materials for Environmental Analysis T2 - Analytica Conference 2002 CY - Munich, Germany DA - 2002-04-23 PY - 2002 SP - 1(?) AN - OPUS4-1389 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Lüders, Ch. T1 - Bestimmung von Nitroaromaten mittels Micellarer Elektrokinetischer Chromatografie (MEKC) T2 - Analytica Conference 2000 CY - Munich, Germany DA - 2000-04-11 PY - 2000 AN - OPUS4-5741 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas T1 - Kopplungsmöglichkeiten der HPLC mit der Massenspektrometrie T2 - GDCh-Kurs 312/99: Hochleistungsflüssigchromatografie CY - Berlin, Germany DA - 1999-09-06 PY - 1999 AN - OPUS4-5711 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Lüders, Ch. T1 - Massenspektrometrische Untersuchungen von Alkylphenolen mit Atmospheric Pressure Ionization T2 - DGMS-Jahrestagung CY - Berlin, Germany DA - 2000-02-27 PY - 2000 AN - OPUS4-5756 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas T1 - Einführung in aktuelle HPLC/MS-Kopplungselekrophorese T2 - HPCL-Grundkurs CY - Berlin, Germany DA - 2000-09-04 PY - 2000 AN - OPUS4-5757 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Lüders, Ch. A1 - Danne, O. A1 - Frei, U. T1 - Massenspektrometrische Untersuchungen von Phosphatidylcholin-Subspezies mit HPLC/MS T2 - DGMS-Jahresaustausch CY - Berlin, Germany DA - 2000-02-27 PY - 2000 AN - OPUS4-5758 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Walther, Wolfgang A1 - Rohde, Gabriele T1 - Einsatz der Kapillarelektrophorese für die Zertifizierung eines Referenzmaterials "Pentachlorphenol in Boden" T2 - 1. Conference über Ionen-Analyse (CIA) CY - Berlin, Germany DA - 2001-02-21 PY - 2001 AN - OPUS4-5825 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Peplinski, T. A1 - Walther, Wolfgang T1 - HPLC/MS-Analyse nichtionischer und anionischer Tensidgemische mit Elektrospray-Ionisation T2 - 28. Diskussionstagung der AG-MS CY - Tübingen, Germany DA - 1995-06-06 PY - 1995 AN - OPUS4-5656 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Peplinski, T. T1 - MS/MS-investigations of wood preservatives and surfactants by electrospray ionisation (ESI) T2 - International Symposium "Chromatography and Mass Spectrometry in Environmental Analysis CY - Saint Petersburg, Russia DA - 1994-10-15 PY - 1994 AN - OPUS4-5643 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Walther, Wolfgang A1 - Peplinski, T. T1 - HPLC/MS-Untersuchungen an Holzschutzmittelwirkstoffen mittels "Atmospheric Pressure" - Ionisationstechniken T2 - InCom '95 CY - Düsseldorf, Germany DA - 1995-03-20 PY - 1995 AN - OPUS4-5646 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas T1 - Kopplungsmöglichkeiten der HPLC mit der Massenspektrometrie T2 - GDCh-Kurs "HPLC" CY - Berlin, Germany DA - 1994-09-15 PY - 1994 AN - OPUS4-5612 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas T1 - Einsatz der HPLC/MS-Kopplung in der Tensidanalytik T2 - 7. Jenaer Umwelt-Analytik-Tage CY - Jena, Germany DA - 1996-04-02 PY - 1996 AN - OPUS4-5616 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Buge, Hans-Gerhard A1 - Win, Tin T1 - Determination of pentachlorphenol (PCP) in wood using various analytical techniques T2 - EUROLAB-Sitzung CY - Berlin, Germany DA - 1996-06-15 PY - 1996 AN - OPUS4-5665 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Buge, Hans-Gerhard A1 - Win, Tin T1 - Determination of pentachlorphenol (PCP in wood using various analytical techniques T2 - EUROLAB-Sitzung CY - Berlin, Germany DA - 1996-06-15 PY - 1996 AN - OPUS4-5666 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Braeutigam, P. A1 - Franke, M. A1 - Schneider, Rudolf A1 - Lehmann, Andreas A1 - Stolle, A. A1 - Ondruschka, B. T1 - Degradation of carbamazepine in environmentally relevant concentrations in water by hydrodynamic-acoustic-cavitation (HAC) N2 - The antiepileptic drug carbamazepine is one of the most abundant pharmaceuticals in the German aquatic environment. The effect of low carbamazepine concentrations (1–50 µg L-1) is discussed controversially, but ecotoxicological studies revealed reproduction toxicity, decreased enzymatic activity and bioaccumulation in different test organisms. Therefore, as a preventive step, an efficient and cost-effective technique for wastewater treatment plants is needed to stop the entry of pharmaceuticals into the aquatic environment. Cavitation, the formation, growth, and subsequent collapse of gas- or vapor-filled bubbles in fluids, was applied to solve this problem. The technique of Hydrodynamic-Acoustic-Cavitation was used showing high synergistic effect. Under optimized conditions carbamazepine (5 µg L-1) was transformed by pseudo-first order kinetics to an extent of >96% within 15 min (27% by hydrodynamic cavitation, 33% by acoustic cavitation). A synergistic effect of 63% based on the sum of the single methods was calculated. Carbamazepine concentrations were monitored by a sensitive and selective immunoassay and after 60 min no known metabolites were detectable by LC-MS/MS. KW - Carbamazepine KW - Degradation KW - Hydrodynamic cavitation KW - Acoustic cavitation KW - ELISA PY - 2012 U6 - https://doi.org/10.1016/j.watres.2012.02.013 SN - 0043-1354 VL - 46 IS - 7 SP - 2469 EP - 2477 PB - Elsevier CY - Amsterdam AN - OPUS4-25735 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Bahlmann, Arnold A1 - Weller, Michael G. A1 - Panne, Ulrich A1 - Schneider, Rudolf T1 - Determination of Carbamazepine, Sulfmaethoxazole and Cocaine in Surface Water in Berlin by LC-MS/MS and ELISA T2 - HPLS Tagung CY - Budapest, Hungary DA - 2011-06-19 PY - 2011 AN - OPUS4-24525 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Gärtner, Stefanie A1 - Schneider, Rudolf T1 - Bestimmung von Gallensäuren mittels HPLC-MS/MS T2 - 8. Deutsches BioSensor Symposium CY - Wildau, Germany DA - 2013-03-10 PY - 2013 AN - OPUS4-27816 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Gierke, Thorsten T1 - Analyse von Azofarbstoffen mittels Kapillarelektrophorese (CE) und Flüssigchromatographie (HPLC) T2 - CIA 2005 (Conference Ion Analysis), Hotel Berlin CY - Berlin, Germany DA - 2005-04-06 PY - 2005 AN - OPUS4-7518 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Reinsch, Martin A1 - Töpfer, Antje A1 - Lehmann, Andreas A1 - Nehls, Irene T1 - Determination of ochratoxin A in wine by liquid chromatography tandem mass spectrometry after combined anion-exchange/reversed-phase clean-up N2 - Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus ochraceus and Penicillium verrucosum. It has been found and analysed in several foods and feeds. Owing to its toxicity and occurrence in food and feed, the European Community has issued directives and some countries have their own regulations for OTA contents in food, feed and beverages. This work describes a method for the determination of OTA in mulled and red wine. It is based on combined anion exchange/reversed-phase clean-up and was analysed by liquid chromatography coupled with tandem mass spectrometry (multiple reaction monitoring). The method was validated with natural contaminated and spiked wine samples with OTA contents from 1.34 to 3.48 g kg–1. Owing to its accuracy, good reproducibility and repeatability, this easy method is a good alternative to liquid chromatography–fluorescence detection methods. KW - Ochratoxin A KW - Wine KW - Tandem mass spectrometry KW - Solid-phase extraction KW - Anion exchange KW - Liquid chromatography PY - 2005 U6 - https://doi.org/10.1007/s00216-005-3097-4 SN - 1618-2642 SN - 1618-2650 VL - 381 IS - 8 SP - 1592 EP - 1595 PB - Springer CY - Berlin AN - OPUS4-10306 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Walther, Wolfgang A1 - Nehls, Irene T1 - Bestimmung von polaren nitroaromatischen Verbindungen mittels Elektrospray-MS/MS T2 - Analytica Conference 2006 CY - Munich, Germany DA - 2006-04-25 PY - 2006 AN - OPUS4-12229 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Hoffmann, Kristin A1 - Schneider, Rudolf T1 - LC-MS Non-Target Screening von Oberflächenwasserproben auf relevante Kreuzreaktanten in einem Immonoassay für Sulfamethoxazol (SMX) T2 - LC-MS in der Umweltanalytik CY - Leipzig, Germany DA - 2012-06-11 PY - 2012 N1 - Geburtsname von Hoffmann, Kristin: Petsch, K. - Birth name of Hoffmann, Kristin: Petsch, K. AN - OPUS4-26647 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Tamschick, S. A1 - Rozenblut-Kościsty, B. A1 - Ogielska, M. A1 - Lehmann, Andreas A1 - Lymberakis, P. A1 - Hoffmann, F. A1 - Lutz, I. A1 - Schneider, Rudolf A1 - Kloas, W. A1 - Stöck, M. T1 - Impaired gonadal and somatic development corroborate vulnerability differences to the synthetic estrogen ethinylestradiol among deeply diverged anuran lineages N2 - Amphibians are undergoing a global decline. One poorly investigated reason could be the pollution of aquatic habitats by endocrine disrupting compounds (EDCs). We tested the susceptibility to the synthetically stabilized estrogen 17α-ethinylestradiol (EE2) in three deeply diverged anuran species, differing in sex determination systems, types of gonadogenesis and larval ecologies. To understand whether data from the amphibian model Xenopus laevis (Pipidae) are analogous and applicable to only distantly related non-model amphibians, tadpoles of X. laevis, Hyla arborea (Hylidae) and Bufo viridis (Bufonidae) were simultaneously exposed to 50, 500 and 5000 ng/L EE2 from hatching until completion of metamorphosis, using a flow-through-system under identical experimental conditions. Comparing molecularly established genetic with histologically assessed phenotypic sex in all species, we have recently shown that EE2 provoked numerous genetic-male-to-phenotypic-female sex reversals and mixed sex individuals, confirming overall its expected feminizing effect. In the present study, we focus on the influence of EE2 on gonadal and somatic development. Anatomy and histology revealed several species-specific effects. In both non-model species, H. arborea and B. viridis, high numbers of anatomically impaired gonads were observed. In H. arborea, exposed to 5000 ng/L EE2, numerous underdeveloped gonads were detected. Whereas EE2 did not alter snout-to-vent length and body weight of X. laevis metamorphs, H. arborea showed a treatment-dependent decrease, while B. viridis exhibited an increase in body weight and snout-to-vent length. Apart from a concentration-dependent occurrence of yellowish skin color in several H. arborea, no organ-specific effects were detected. Since EE2 ubiquitously occurs in many aquatic ecosystems and affects sexual and somatic development, among EDCs, it may indeed contribute to amphibian decline. The inter-species variation in developmental EE2-effects corroborates species-specific vulnerability differences towards EDCs between deeply diverged amphibian groups KW - Endocrine disruption KW - Ethinylestradiol KW - Mass spectrometry PY - 2016 U6 - https://doi.org/10.1016/j.aquatox.2016.07.001 SN - 0166-445X SN - 1879-1514 VL - 177 SP - 503 EP - 514 PB - Elsevier AN - OPUS4-37633 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Gärtner, Stefanie A1 - Schneider, Rudolf T1 - Determination of bile acids in wastewater and surface water samples in Berlin by LC-MS/MS and ELISA T2 - 30th International Symposium on Chromatography (ISC) 2014 Salzburg CY - Salzburg, Austria DA - 2014-09-14 PY - 2014 AN - OPUS4-31643 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Oberleitner, Lidia A1 - Eremin, Sergei A. A1 - Schneider, Rudolf T1 - Synthesis and verification of new fluorescence polarization immunoassay tracers for carbamazepine by LC-MS T2 - 30th International Symposium on Chromatography (ISC) 2014 Salzburg CY - Salzburg, Austria DA - 2014-09-14 PY - 2014 AN - OPUS4-31644 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Bahlmann, Arnold A1 - Weller, Michael G. A1 - Panne, Ulrich A1 - Schneider, Rudolf T1 - Determination of Carbamazepine, Sulfamethoxazole and Cocaine in Surface Water in Berlin by LC-MS/MS and ELISA T2 - HPLS-Tagung CY - Budapest, Hungary DA - 2011-06-19 PY - 2011 AN - OPUS4-23938 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Baldofski, Stefanie A1 - Hoffmann, Holger A1 - Lehmann, Andreas A1 - Breitfeld, Stefan A1 - Garbe, L.-A. A1 - Schneider, Rudolf T1 - Enzyme-linked immunosorbent assay (ELISA) for the anthropogenic marker isolithocholic acid in water N2 - Bile acids are promising chemical markers to assess the pollution of water samples with fecal material. This study describes the optimization and validation of a direct competitive enzyme-linked immunosorbent assay for the bile acid isolithocholic acid (ILA). The quantification range of the optimized Assay was between 0.09 and 15 mg/L. The assay was applied to environmental water samples. Most studies until now were focused on bile acid fractions in the particulate phase of water samples. In order to avoid tedious sample preparation, we undertook to evaluate the dynamics and significance of ILA levels in the aqueous phase. Very low concentrations in tap and surface water samples made a pre-concentration step necessary for this matrix as well as for wastewater treatment plant (WWTP) effluent. Mean recoveries for spiked water samples were between 97% and 109% for tap water and WWTP influent samples and between 102% and 136% for WWTP effluent samples. 90th percentiles of intra-plate and inter-plate coefficients of variation were below 10% for influents and below 20% for effluents and surface water. ILA concentrations were quantified in the range of 33-72 mg/L in influent, 21-49 ng/L in effluent and 18-48 ng/L in surface water samples. During wastewater Treatment the ILA levels were reduced by more than 99%. ILA concentrations of influents determined by ELISA and LC-MS/MS were in good agreement. However, findings in LC-ELISA experiments suggest that the true ILA levels in concentrated samples are lower due to interfering effects of matrix compounds and/or cross-reactants. Yet, the ELISA will be a valuable tool for the performance check and comparison of WWTPs and the localization of fecal matter input into surface waters. KW - ELISA KW - LC-MS/MS KW - Bile acids KW - Fecal marker KW - Surface water KW - Wastewater PY - 2016 U6 - https://doi.org/10.1016/j.jenvman.2016.08.023 SN - 0301-4797 VL - 182 SP - 612 EP - 619 AN - OPUS4-37668 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Oberleitner, Lidia A1 - Eremin, S.A. A1 - Lehmann, Andreas A1 - Garbe, L.-A. A1 - Schneider, Rudolf T1 - Fluorescence polarization immunoassays for carbamazepine - comparison of tracers and formats N2 - For the antiepileptic drug and anthropogenic marker carbamazepine (CBZ), a fast and cost-effective immunoassay based on fluorescence polarization (FPIA) was developed. The required fluorophore conjugates were synthesized from different fluorescein and CBZ derivatives. The most suitable tracer was CBZ–triglycine–5-(aminoacetamido)fluorescein. Additionally, the applicability of the assay in tubes and on microtiter plates was tested. The first format can be performed in a portable instrument and therefore can be applied in field measurements. The measurement of an individual sample can be carried out within 4 min. This assay shows a measurement range of 2.5–1000 µg L-1 and a test midpoint (or IC50) of 36 µg L-1. The FPIA performed on microtiter plates is useful for the assay development and is suitable for a very high throughput (up to 24 samples in 20 min). The test midpoint of this assay is 13 µg L-1 and the measurement range is 1.5–300 µg L-1. Furthermore, this assay requires smaller sample volumes and less reagents, including the crucial amount of antibody. The applicability of both assays to spiked surface water samples was evaluated. The recovery rates vary between 66–110% on microtiter plates and 81–140% in tubes. PY - 2015 U6 - https://doi.org/10.1039/c5ay00617a SN - 1759-9660 SN - 1759-9679 VL - 7 IS - 14 SP - 5854 EP - 5861 PB - RSC Publ. CY - Cambridge AN - OPUS4-33821 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Sin, DWM. A1 - Wong, YC. A1 - Lehmann, Andreas A1 - Schneider, Rudolf A1 - Kakoulides, E. A1 - Lin, TT. A1 - Liu, QD. A1 - Cabillic, J. A1 - Lardy-fontan, S. A1 - Nammoonnoy, J. T1 - CCQM-K126: low polarity organic in water: carbamazepine in surface water N2 - This study aimed to assess the measurement capabilities of participating National Metrology Institutes/ Designated Institutes (NMIs/DIs) and expert laboratories in determining of low-polarity organics in surface water. This comparison was organized by Government Laboratory, Hong Kong (GLHK). At the CCQM Organic Analysis Working Group (OAWG) Meeting held in November 2012 in Hong Kong, GLHK initially proposed a CCQM key comparison and a parallel pilot study programme on pharmaceuticals in surface water. Further discussion at the CCQM Meeting held in April 2014 in Paris, the OAWG approved a CCQM Track C comparison (CCQM-K126) on low polarity pharmaceuticals in surface water. In the meeting, the programme was supported by more than six National Metrology Institutes/ Designated Institutes (NMIs/ DIs). CCQM-K126 officially commenced in July 2014 and had registration from nine NMIs/DIs. Participants were provided two bottles (40 mL each) of surface water and were requested to determine the mass fraction of spiked carbamazepinein in surface water. The coordinator received nine sets of results from eight NMIs/DIs in February 2015. Apart from one using an immunoassay technique, all participants applied isotope dilution liquid chromatography-tandem mass spectrometry (ID-LCMS/MS) technique as their determination technique. KW - CCQM-K126 KW - ELISA KW - Mass spectrometry PY - 2017 U6 - https://doi.org/10.1088/0026-1394/54/1A/08030 SN - 0026-1394 SN - 1681-7575 VL - 54 IS - Suppl. S SP - Article 08030, 1 EP - 56 PB - IOP Publ. CY - Bristol AN - OPUS4-44831 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Tamschick, S. A1 - Rozenblut-Kościsty, B. A1 - Ogielska, M. A1 - Lehmann, Andreas A1 - Lymberakis, P. A1 - Hoffmann, F. A1 - Lutz, I. A1 - Kloas, W. A1 - Stöck, M. T1 - Sex reversal assessments reveal different vulnerability to endocrine disruption between deeply diverged anuran lineages N2 - Multiple anthropogenic stressors cause worldwide amphibian declines. Among several poorly investigated causes is global pollution of aquatic ecosystems with endocrine disrupting compounds (EDCs). These substances interfere with the endocrine system and can affect the sexual development of vertebrates including amphibians. We test the susceptibility to an environmentally relevant contraceptive, the artificial estrogen 17α-ethinylestradiol (EE2), simultaneously in three deeply divergent systematic anuran families, a model-species, Xenopus laevis (Pipidae), and two non-models, Hyla arborea (Hylidae) and Bufo viridis (Bufonidae). Our new approach combines synchronized tadpole exposure to three EE2-concentrations (50, 500, 5,000 ng/L) in a flow-through-system and pioneers genetic and histological sexing of metamorphs in non-model anurans for EDC-studies. This novel methodology reveals striking quantitative differences in genetic-male-to-phenotypic-female sex reversal in non-model vs. model species. Our findings qualify molecular sexing in EDC-analyses as requirement to identify sex reversals and state-of-the-art approaches as mandatory to detect speciesspecific vulnerabilities to EDCs in amphibians. KW - Endocrine disruption KW - 17α-ethinylestradiol (EE2) KW - Mass spectrometry KW - Sex reversal PY - 2016 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:b43-357797 SN - 2045-2322 VL - 6 SP - Article No. 23825, 1 EP - 8 PB - Nature publishing group AN - OPUS4-35779 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lehmann, Andreas A1 - Schneider, Rudolf T1 - Matrixeinflüsse bei der Bestimmung von Arzneimittelrückständen mittels LC-MS/MS in Oberflächenwasserproben bei Konzentrationen im ppt-Bereich N2 - Zur Bestimmung von Arzneimittelrückständen in Oberflächengewässern wird häufig die LC-MS/MS als Methode der Wahl eingesetzt. Obwohl sich dieses Nachweisverfahren durch eine hohe Empfindlichkeit und Selektivität auszeichnet, gibt es vor allem bei geringen Substanzkonzentrationen häufig störende Einflüsse durch Matrixeffekte. Diese Matrixeffekte können unterschiedliche Ursachen haben. Einige Matrixeffekte werden am Beispiel des Wirkstoffs Carbamazepin erläutert. Carbamazepin wird durch Kläranlagen nur partiell aus dem Abwasser entfernt und ist im Spurenbereich auch noch im Trinkwasser nachzuweisen. Wenn für die LC-MS/MS wasserbasierte Anteile in der mobilen Phase genutzt werden, befinden sich trotz Verwendung einer Labor-Reinstwasseranlage in der mobilen Phase noch kleinste Konzentrationen von Carbamazepin, welche einen Blindwert im unteren ppt-Bereich verursachen. Weitere Matrixeffekte treten durch die unterschiedliche Qualität der Oberflächenwasserproben auf. Koelutionen von weiteren Inhaltstoffen zeitgleich mit dem Zielanalyten führen zu einer Diskriminierung des Meßsignals. Verbindungen, die den gleichen Massenübergang bei nahezu identischer Retentionszeit zeigen, erfordern eine reproduzierbare chromatographische Vortrennung oder die Wahl einer alternativen HPLC-Säule. T2 - ANAKON 2015 CY - Graz, Austria DA - 23.03.2015 KW - Arzneimittelrückstände in Oberflächenwasserproben KW - HPLC-MS/MS KW - Massenspektrometrie KW - Carbamazepin PY - 2015 AN - OPUS4-35793 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Carl, Peter A1 - Sarma, Dominik A1 - Gregório, Bruno J. R. A1 - Hoffmann, Kristin A1 - Lehmann, Andreas A1 - Rurack, Knut A1 - Schneider, Rudolf T1 - Wash-Free Multiplexed Mix-and-Read Suspension Array Fluorescence Immunoassay for Anthropogenic Markers in Wastewater N2 - Pharmaceuticals, certain food ingredients, and mammalian endogenous metabolic products in wastewater are mostly of human origin. They are anthropogenic markers. Proper knowledge of their levels in wastewater helps to track sources of pollutants in natural waters and allows for calculation of removal efficiencies in wastewater Treatment plants. Here, we describe the development and application of an indirect competitive, multiplexing suspension Array fluorescence immunoassay (SAFIA) for the detection of carbamazepine (CBZ), diclofenac (DCF), caffeine (CAF), and isolithocholic acid (ILA) in wastewater, covering those classes of anthropogenic markers. The assay consists of haptens covalently conjugated to fluorescence-encoded polystyrene core/silica shell microparticles to create a site for competitive binding of the antibodies (Abs). Bound Abs are then stained with fluorophore-labeled Abs. Encoding and signaling fluorescence of the particles are determined by an automated flow cytometer. For compatibility of the immunoassay with the 96-well microtiter plate format, a stop reagent, containing formaldehyde, is used. This enables a wash-free procedure while decreasing time-to-result. Detection limits of 140 ± 40 ng/L for CBZ, 180 ± 110 ng/L for CAF, 4 ± 3 ng/L for DCF, and 310 ± 70 ng/L for ILA are achieved, which meet the sensitivity criteria of wastewater analysis. We demonstrate the applicability of SAFIA to real wastewater samples from three different wastewater Treatment plants, finding the results in good agreement with LC-MS/MS. Moreover, the accuracy in general exceeded that from classical ELISAs. We therefore propose SAFIA as a quick and reliable approach for wastewater analysis meeting the requirements for process analytical technology. KW - Suspension Array KW - Immunoassay KW - Carbamazepine KW - Caffeine KW - Diclofenac KW - SAFIA PY - 2019 U6 - https://doi.org/10.1021/acs.analchem.9b03040 SN - 0003-2700 VL - 91 IS - 20 SP - 12988 EP - 12966 PB - ACS Publications AN - OPUS4-49305 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -