TY - JOUR A1 - Knabe, C. A1 - Berger, Georg A1 - Gildenhaar, Renate A1 - Meyer, J. A1 - Howlett, C. A1 - Markovic, B. A1 - Zreiqat, H. T1 - Effect of rapidly resorbable calcium phosphates and a calcium phosphate bone cement on the expression of bone-related genes and proteins in vitro JF - Journal of biomedical materials research / A N2 - The use of biodegradable bone substitutes is advantageous for alveolar ridge augmentation because it avoids second-site surgery for autograft harvesting. This study examines the effect of novel, rapidly resorbable calcium phosphates and a calcium phosphate bone cement on the expression of bone-related genes and proteins by human bone-derived cells (HBDCs) and compares this behavior to that of tricalciumphosphate (TCP). Test materials were -TCP, two materials with a crystalline phase Ca2KNa(PO4)2 and with a small amorphous portion containing either magnesium potassium phosphate (material denominated GB14) or silica phosphate (material denominated GB9), and a calcium phosphate bone cement (material denominated Biocement D). HBDCs were grown on the substrata for 3, 7, 14, and 21 days, counted, and probed for various mRNAs and proteins (type I collagen, osteocalcin, osteopontin, osteonectin, alkaline phosphatase, and bone sialoprotein). All substrates supported continuous cellular growth for 21 days. In the presence of GB14 and Biocement D specimens cell proliferation was reduced and cell differentiation increased. At day 21, the greatest number of cells was found on GB9 expressing significantly higher levels of bone-related proteins than cells grown on all other surfaces. Because all novel materials facilitated the expression of the osteoblastic phenotype at least as much as TCP and the polystyrene control, these biomaterials can be regarded as excellent candidate bone substitute materials. GB9 induced the highest proliferation and cellular differentiation after 21 days of incubation, suggesting that this material may possess a higher potency for enhancing osteogenesis than TCP. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 69A: 145-154, 2004 KW - Calcium phosphate ceramics KW - Human bone-derived cells KW - Cell-biomaterial interactions KW - Calcium phosphate cements KW - In situ hybridization PY - 2004 DO - https://doi.org/10.1002/jbm.a.20131 SN - 1549-3296 SN - 1552-4965 VL - 69 IS - 1 SP - 145 EP - 154 PB - Wiley CY - Hoboken, NJ AN - OPUS4-3502 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gildenhaar, Renate A1 - Berger, Georg A1 - Knabe, C. A1 - Howlett, C. A1 - Markovic, B. A1 - Klar, F. A1 - Zreiqat, H. T1 - The Effect of Different Dental Titanium and Calcium Phosphate Implant Surfaces on the Expression of Bone-Related Genes and Proteins in vitro T2 - Transactions of the Sixth World Biomaterials Congress T2 - 6th World Biomaterials Congress CY - Kamuela, Big Island, HI, USA DA - 2000-05-15 KW - Dental Implant Surfaces PY - 2000 VL - 1 SP - 238 PB - Society for Biomaterials CY - Minneapolis, Minn. AN - OPUS4-869 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gildenhaar, Renate A1 - Berger, Georg A1 - Knabe, C. A1 - Howlett, C. A1 - Markovic, B. A1 - Reif, D. A1 - Zreiqat, H. T1 - The Functional Expression of Human Bone Derived Cells Grown on Rapidly Resorbable Calcium Phosphate Ceramics T2 - Transactions of the Sixth World Biomaterials Congress T2 - 6th World Biomaterials Congress CY - Kamuela, Big Island, HI, USA DA - 2000-05-15 KW - Calcium Phosphate Ceramics PY - 2000 SP - 970 PB - Society for Biomaterials CY - Minneapolis, Minn. AN - OPUS4-870 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Knabe, C. A1 - Howlett, C. A1 - Markovic, B. A1 - Gildenhaar, Renate A1 - Berger, Georg A1 - Zreiqat, H. T1 - The functional expression of human osteoblasts grown on resorbable calcium phosphates JF - Key engineering materials N2 - The use of biodegradable bone substitutes is advantageous for alveolar ridge augmentation, since it avoids second-site surgery for autograft harvesting. This study examines the effect of novel, rapidly resorbable calcium phosphates on the expression of bone-related genes and proteins by human bone derived cells (HBDC) and compares this behavior to that of tricalciumphosphate (TCP). Test materials were -TCP, a newly synthesized chemical Ca2KNa(PO4)2 (material denominated R17), two glassy crystalline materials with a crystalline phase Ca2KNa(PO4)2 and in addition a small amorphous portion containing either magnesium potassium phosphate (material denominated GB14) or silica phosphate (material denominated GB9). HBDC was grown on the substrates for 3, 5, 7, 14 and 21 days, counted and probed for various mRNAs and proteins (collagen type I, osteocalcin, osteopontin, osteonectin, alkaline phosphatase and bone sialoprotein). All substrates supported continuous cellular growth for 21 days. At day 21, R17, GB9, TCP and GB14. T2 - 15th International Symposium on Ceramics in Medicine CY - Sydney, NSW, Australia DA - 2002-12-04 PY - 2002 DO - https://doi.org/10.4028/www.scientific.net/KEM.240-242.679 SN - 1013-9826 VL - 240-242 SP - 679 EP - 682 PB - Trans Tech Publ. CY - Aedermannsdorf AN - OPUS4-1891 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Knabe, C. A1 - Howlett, C. A1 - Markovic, B. A1 - Gildenhaar, Renate A1 - Berger, Georg A1 - Reif, D. A1 - Zreiqat, H. T1 - The functional expression of human bone derived cells grown on resorbable bioactive glass ceramics T2 - 28th annual meeting & exposition T2 - 28th Annual Meeting & Exposition of the Society for Biomaterials CY - Tampa, FL, USA DA - 2002-04-23 PY - 2002 SP - 706 PB - Society for Biomaterials AN - OPUS4-1892 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -