TY - JOUR A1 - Richter, Janine A1 - Fettig, Ina A1 - Piechotta, Christian A1 - Philipp, Rosemarie A1 - Jakubowski, Norbert T1 - Tributylzinn in Gesamtwasserproben - Entwicklung eines Referenzverfahrens für die EU-Wasserrahmenrichtlinie JF - GIT : Labor-Fachzeitschrift PY - 2013 SN - 0016-3538 IS - 9 SP - 2 EP - 4 PB - GIT-Verlag CY - Darmstadt AN - OPUS4-29040 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Müller, Larissa A1 - Panne, Ulrich A1 - Roos, P.H. A1 - Drescher, Daniela A1 - Traub, Heike A1 - Kneipp, Janina T1 - Tools for metallomics: ICP-MS T2 - 4th International Symposium on Metallomics 2013 T2 - 4th International Symposium on Metallomics 2013 CY - Oviedo, Spain DA - 2013-07-08 PY - 2013 AN - OPUS4-28817 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Lindner, Uwe A1 - Lingott, Jana A1 - Richter, Silke A1 - Jakubowski, Norbert A1 - Panne, Ulrich T1 - Speciation of gadolinium in surface water samples and plants by hydrophilic interaction chromatography hyphenated with inductively coupled plasma mass spectrometry JF - Analytical and bioanalytical chemistry N2 - Hydrophilic interaction chromatography (HILIC) coupled with inductively coupled plasma mass spectrometry (ICP-MS) was optimized for speciation analysis of gadolinium-based contrast agents in environmental samples, in particular surface river waters and plants. Surface water samples from the Teltow channel, near Berlin, were investigated over a distance of 5 km downstream from the influx of a wastewater treatment plant. The total concentration of gadolinium increased significantly from 50 to 990 ngL-1 due to the influx of the contrast agents. After complete mixing with the river water, the concentration remained constant over a distance of at least 4 km. Two main substances [Dotarem® (Gd-DOTA) and Gadovist® (Gd-BT-DO3A)] have been identified in the river water using standards. A gadolinium-based contrast agent, possibly Gd-DOTA (Dotarem®), was also detected in water plant samples taken from the Teltow channel. Therefore, uptake of contrast agents [Gadovist® (Gd-BTDO3A), Magnevist® (Gd-DTPA), Omniscan® (Gd-DTPA-BMA), Dotarem® (Gd-DOTA), and Multihance® (Gd-BOPTA)] by plants was investigated in a model experiment using Lepidium sativum (cress plants). HILIC–ICP-MS was used for identification of different contrast agents, and a first approach for quantification using aqueous standard solutions was tested. For speciation analysis, all investigated contrast agents could be extracted from the plant tissues with a recovery of about 54 % for Multihance® (Gd-BOPTA) up to 106 % for Gadovist® (Gd-BT-DO3A). These experiments demonstrate that all contrast agents investigated are transported from the roots to the leaves where the highest content was measured. KW - Gadolinium-based contrast agents KW - Hydrophilic interaction chromatography (HILIC) KW - Speciation KW - Inductively coupled plasma mass spectrometry (ICP-MS) KW - Plants KW - Surface water PY - 2013 DO - https://doi.org/10.1007/s00216-012-6643-x SN - 1618-2642 SN - 1618-2650 VL - 405 IS - 6 SP - 1865 EP - 1873 PB - Springer CY - Berlin AN - OPUS4-27929 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Sugiyama, Kaori A1 - Traub, Heike A1 - Panne, Ulrich A1 - Rampler, Evelyn A1 - Köllensperger, G. A1 - Drescher, Daniela A1 - Giesen, Charlotte A1 - Kneipp, Janina T1 - Single Cell analysis by Elemental Mass Spectrometry T2 - AnalytiX 2013 T2 - AnalytiX 2013 CY - Suzhou, China DA - 2013-03-21 PY - 2013 AN - OPUS4-27963 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Shigeta, K. A1 - Koellensperger, G. A1 - Rampler, E. A1 - Traub, Heike A1 - Rottmann, L. A1 - Panne, Ulrich A1 - Okino, A. A1 - Jakubowski, Norbert T1 - Sample introduction of single selenized yeast cells (Saccharomyces cerevisiae) by micro droplet generation into an ICP-sector field mass spectrometer for label-free detection of trace elements JF - Journal of analytical atomic spectrometry N2 - We have applied a micro droplet generator (µDG) for sample introduction of single selenized yeast cells into a sector field ICP-MS, which was operated in a fast scanning mode with sampling rates of up to 10 kHz, to measure single cells time resolved with 100 µs integration time. Selenized yeast cells have been used as a model system for preliminary investigation. The single cells to be measured have been embedded into droplets and it will be shown that the time duration of a single cell event always is about 400 to 500 µs, and thus comparable to the time duration of a droplet without a cell. A fixed droplet generation rate of 50 Hz produced equidistant signals in time of each droplet event and was advantageous to separate contribution from background and blank from the analytical signal. Open vessel digestion and a multielement analysis were performed with washed yeast cells and absolute amounts per single cell were determined for Na (0.91 fg), Mg (9.4 fg), Fe (5.9 fg), Cu (0.54 fg), Zn (1.2 fg) and Se (72 fg). Signal intensities from single cells have been measured for the elements Cu, Zn and Se, and histograms were calculated for about 1000 cell events. The mean elemental sensitivities measured here range from 0.7 counts per ag (Se) to 10 counts per ag (Zn) with RSD's from 49% (Zn) to 69% (Se) for about 1000 cell events. PY - 2013 DO - https://doi.org/10.1039/c3ja30370e SN - 0267-9477 SN - 1364-5544 VL - 28 IS - 5 SP - 637 EP - 645 PB - Royal Society of Chemistry CY - London AN - OPUS4-29448 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Munter, L.M. A1 - Sieg, H. A1 - Bethge, T. A1 - Liebsch, F. A1 - Bierkandt, Frank A1 - Schleeger, M. A1 - Bittner, H.J. A1 - Heberle, J. A1 - Jakubowski, Norbert A1 - Hildebrand, P.W. A1 - Multhaup, G. T1 - Model peptides uncover the role of the beta-secretase transmembrane sequence in metal ion mediated oligomerization JF - Journal of the American chemical society N2 - The β-secretase or β-site amyloid precursor protein cleaving enzyme 1 (BACE1) is the enzyme responsible for the formation of amyloid-β peptides, which have a major role in Alzheimer pathogenesis. BACE1 has a transmembrane sequence (TMS), which makes it unique among related proteases. We noticed that the BACE1 TMS contains an uncommon sulfur-rich motif. The sequence MxxxCxxxMxxxCxMxC spans the entire TMS, resembles metal ion binding motifs, and is highly conserved among homologues. We used a synthetic 31-mer model peptide comprising the TMS to study metal ion binding and oligomerization. Applying diverse biochemical and biophysical techniques, we detected dimer and trimer formation of the TMS peptide with copper ions. Replacement of the central Cys466 by Ala essentially abolished these effects. We show that the peptide undergoes a redox reaction with copper ions resulting in a disulfide bridge involving Cys466. Further, we find peptide trimerization that depends on the presence of monovalent copper ions and the sulfhydryl group of Cys466. We identified Cys466 as a key residue for metal ion chelation and to be the core of an oligomerization motif of the BACE1-TMS peptide. Our results demonstrate a novel metal ion controlled oligomerization of the BACE1 TMS, which could have an enormous therapeutic importance against Alzheimer disease. KW - Enzymes KW - Amyloid formation KW - Alzheimer disease KW - Copper PY - 2013 DO - https://doi.org/10.1021/ja410812r SN - 0002-7863 SN - 1520-5126 VL - 135 IS - 51 SP - 19354 EP - 19361 PB - American Chemical Society CY - Washington, DC AN - OPUS4-30543 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Moreno-Gordaliza, E. A1 - Esteban-Fernández, D. A1 - Giesen, Ch. A1 - Lehmann, K. A1 - Lázaro-Fernández, A. A1 - Canas, B. A1 - Tejedor, A. A1 - Scheler, Ch. A1 - Linscheid, M. A1 - Gómez-Gómez, M. T1 - LA-ICP-MS for the detection of (semi)metallo-proteins in biological samples after PAGE / 2-DE separations T2 - Vortrag bei Prof. Ogra, University of Pharmaceutical T2 - Vortrag bei Prof. Ogra, University of Pharmaceutical CY - Tokyo, Japan DA - 2013-10-01 PY - 2013 AN - OPUS4-29198 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Moreno-Gordaliza, E. A1 - Esteban-Fernández, D. A1 - Giesen, Ch. A1 - Lehmann, K. A1 - Lázaro-Fernández, A. A1 - Canas, B. A1 - Tejedor, A. A1 - Scheler, Ch. A1 - Linscheid, M. A1 - Gómez-Gómez, M. T1 - LA-ICP-MS for the detection of (semi)metallo-proteins in biological samples after PAGE / 2-DE separations T2 - Vortrag bei Prof. Zuruta, Chuo University T2 - Vortrag bei Prof. Zuruta, Chuo University CY - Tokyo, Japan DA - 2013-10-03 PY - 2013 AN - OPUS4-29201 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Müller, Larissa A1 - Panne, Ulrich A1 - Roos, P.H. A1 - Drescher, Daniela A1 - Traub, Heike A1 - Kneipp, Janina T1 - Imaging of metals in biological and clinical samples by use of LA-ICP-MS: Challenges and limitations! T2 - 9. ASAC JunganalytikerInnen Forum T2 - 9. ASAC JunganalytikerInnen Forum CY - Vienna, Austria DA - 2013-06-21 PY - 2013 AN - OPUS4-28816 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Müller, Larissa A1 - Panne, Ulrich A1 - Roos, P.H. A1 - Drescher, Daniela A1 - Traub, Heike A1 - Kneipp, Janina T1 - Imaging mittels Laser-ablation-ICP-MS T2 - Ringvorlesung Analytik T2 - Ringvorlesung Analytik CY - Berlin, Germany DA - 2013-06-28 PY - 2013 AN - OPUS4-28818 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -