TY - CONF A1 - Jakubowski, Norbert T1 - Einführung in die anorganische Massenspektroskopie T2 - 46. Jahrestagung der Deutschen Gesellschaft für Massenspektrometrie T2 - 46. Jahrestagung der Deutschen Gesellschaft für Massenspektrometrie CY - Berlin, Germany DA - 2013-03-10 PY - 2013 AN - OPUS4-28032 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert T1 - Novel challenges for elemental analysis T2 - Bruker European Sales meeting T2 - Bruker European Sales meeting CY - Berlin, Germany DA - 2012-04-25 PY - 2012 AN - OPUS4-25950 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert T1 - Development of ICP-MS based methods for label free detection of single cells T2 - European Winter Conference on Plasma Spectrochemistry T2 - European Winter Conference on Plasma Spectrochemistry CY - Kraków, Poland DA - 2013-02-09 PY - 2013 AN - OPUS4-27891 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert T1 - Short Course: ICP-MS with Sector Field Devices T2 - 2012 Winter Conference on Plasma Spectrochemistry T2 - 2012 Winter Conference on Plasma Spectrochemistry CY - Tucson, AZ, USA DA - 2012-01-09 PY - 2012 AN - OPUS4-25367 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert T1 - Thermo Fisher Scientific Award in Plasma Spectrochemistry T2 - 2012 Winter Conference on Plasma Spectrochemistry T2 - 2012 Winter Conference on Plasma Spectrochemistry CY - Tucson, AZ, USA DA - 2012-01-09 PY - 2012 AN - OPUS4-25368 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Polatajko, A. A1 - Feldmann, I. A1 - Hayen, H. A1 - Jakubowski, Norbert T1 - Combined application of a laser ablation-ICP-MS assay for screening and ESI-FTICR-MS for identification of a Cd-binding protein in Spinacia oleracea L. after exposure to Cd JF - Metallomics N2 - We have studied the binding of the toxic element Cd to plant proteins and have used for this purpose spinach (Spinacia oleracea L.) plants treated with 50 µM Cd(II) as a model system. Laser ablation ICP-MS has been applied for the screening of Cd-binding proteins after separation by native anodal polyacrylamide gel electrophoresis (AN-PAGE) and electroblotting onto membranes. The main Cd-carrying protein band was isolated and investigated by nano-electrospray ionization–Fourier transform ion cyclotron resonance (FTICR) mass spectrometry after tryptic digestion. By this procedure, the main Cd-binding protein was identified as ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). The latter enzyme has been discussed in the literature to be affected in its activity by oxidative stress induced by Cd. However, in this paper it is demonstrated for the first time that RuBisCO directly binds Cd and thus may be directly altered by this toxic element. A commercially available protein standard was used to verify direct binding of Cd(II) to the protein, even without metabolisation. The resulting metal–protein complex was shown to be stable enough to survive AN-PAGE separation and electroblotting. By the use of size exclusion chromatography coupled with ICP-MS it was demonstrated that the RuBisCO protein standard shows similar metal binding properties to Cd. Furthermore, essential elements such as Mn(II), Fe(II) and Cu(II), which are known to possibly replace the RuBisCO activator Mg(II), were investigated in addition to Zn(II). Again, similar binding properties in comparison to the plant protein were observed. PY - 2011 DO - https://doi.org/10.1039/c1mt00051a SN - 1756-5901 SN - 1756-591X VL - 3 IS - 10 SP - 1001 EP - 1008 PB - RSC Publ. CY - Cambridge AN - OPUS4-24963 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Giesen, Charlotte A1 - Moreno-Gordaliza, E. A1 - Esteban-Fernández, D. A1 - Lehmann, K. A1 - Canas, B. A1 - Tejedor, A. A1 - Scheler, Ch. A1 - Linscheid, M. A1 - Gómez-Gómez, M. M. A1 - Lázaro-Fernández, A. T1 - Interaction of metals with biomolecules: do we have the proper tools? T2 - "Speciation Seminar" Congress Centre T2 - "Speciation Seminar" Congress Centre CY - Montpellier, France DA - 2012-05-29 PY - 2012 AN - OPUS4-27353 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Müller, Larissa A1 - Giesen, Charlotte A1 - Hösl, Simone A1 - Techritz, Sandra A1 - Roos, Albrecht A1 - Scheler, Ch. T1 - LA-ICP-MS an new tool for development of methods for imaging of biological and clinical samples T2 - Projektbesprechung Metallproteins T2 - Projektbesprechung Metallproteins CY - Aberdeen, Scotland DA - 2012-11-14 PY - 2012 N1 - Geburtsname von Müller, Larissa: Wäntig, L. - Birth name of Müller, Larissa: Wäntig, L. N1 - Geburtsname von Hösl, Simone: Hardt, S. - Birth name of Hösl, Simone: Hardt, S. AN - OPUS4-27354 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Müller, Larissa A1 - Jakubowski, Norbert A1 - Hösl, Simone A1 - Scheler, C. A1 - Roos, P.H. A1 - Linscheid, M.W. T1 - Comparison of different chelates for lanthanide labeling of antibodies and application in a Western blot immunoassay combined with detection by laser ablation (LA-)ICP-MS JF - Journal of analytical atomic spectrometry N2 - We have developed lanthanide labeling strategies for antibodies to adapt conventional biochemical workflows like Western blot immunoassays for detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) analysis with a special interest to apply the multi-element capabilities of ICP-MS for the design of multiplexed immunoassays. In this paper the lanthanide labeling of antibodies with MeCAT was investigated and the reaction conditions were optimized for application in a Western blot immunoassay analyzed by LA-ICP-MS. Furthermore, the MeCAT labeling strategy was compared with two other commercially available labeling reagents, MAXPAR™ and SCN-DOTA. As a proof-of-principle experiment chemically induced alterations of cytochrome P450 protein expression were investigated and the suitability of the differentially labeled antibodies for Western blot immunoassays of a complex liver microsomal protein fraction was tested. Limits of detection (LODs) in the lower fmol range were reached in the Western blot application using MeCAT and MAXPAR™ as element labeling reagents, whereas even sub-fmol LODs can be achieved in a dot blot experiment for the pure antibodies. PY - 2012 DO - https://doi.org/10.1039/c2ja30068k SN - 0267-9477 SN - 1364-5544 N1 - Geburtsname von Müller, Larissa: Wäntig, L. - Birth name of Müller, Larissa: Wäntig, L. N1 - Geburtsname von Hösl, Simone: Hardt, S. - Birth name of Hösl, Simone: Hardt, S. VL - 27 IS - 8 SP - 1311 EP - 1320 PB - Royal Society of Chemistry CY - London AN - OPUS4-26256 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Richter, Janine A1 - Fettig, Ina A1 - Piechotta, Christian A1 - Philipp, Rosemarie A1 - Jakubowski, Norbert T1 - Tributylzinn in Gesamtwasserproben - Entwicklung eines Referenzverfahrens für die EU-Wasserrahmenrichtlinie JF - GIT : Labor-Fachzeitschrift PY - 2013 SN - 0016-3538 IS - 9 SP - 2 EP - 4 PB - GIT-Verlag CY - Darmstadt AN - OPUS4-29040 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Müller, Larissa A1 - Techritz, Sandra A1 - Jakubowski, Norbert A1 - Roos, P.H. T1 - A multi-parametric microarray for protein profiling: simultaneous analysis of 8 different cytochromes via differentially element tagged antibodies and laser ablation ICP-MS JF - Analyst N2 - The paper presents a new multi-parametric protein microarray embracing the multi-analyte capabilities of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The combination of high throughput reverse phase protein microarrays with element tagged antibodies and LA-ICP-MS makes it possible to detect and quantify many proteins or biomarkers in multiple samples simultaneously. A proof of concept experiment is performed for the analysis of cytochromes particularly of cytochrome P450 enzymes, which play an important role in the metabolism of xenobiotics such as toxicants and drugs. With the aid of the LA-ICP-MS based multi-parametric reverse phase protein microarray it was possible to analyse 8 cytochromes in 14 different proteomes in one run. The methodology shows excellent detection limits in the lower amol range and a very good linearity of R² ≥ 0.9996 which is a prerequisite for the development of further quantification strategies. KW - Multi-parametric KW - Multiplexing KW - Microarray KW - Immunoassay KW - LA-ICP-MS KW - Cytochrome P450 PY - 2013 DO - https://doi.org/10.1039/c3an00468f SN - 0003-2654 SN - 1364-5528 VL - 138 IS - 21 SP - 6309 EP - 6315 PB - Royal Society of Chemistry CY - Cambridge AN - OPUS4-29275 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert T1 - The Role of Modern Plasma-based Spectroscopies for Development and Characterization of Reference Materials T2 - Winter Conference on Plasma Spectrochemistry T2 - Winter Conference on Plasma Spectrochemistry CY - Fort Myers, FL, USA DA - 2010-01-04 PY - 2010 AN - OPUS4-20838 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Roos, P.H. A1 - Jakubowski, Norbert T1 - Methods for the discovery of low-abundance biomarkers for urinary bladder cancer in biological fluids JF - Bioanalysis N2 - For the study of bladder cancer and the identification of respective tumor markers, blood and, in particular, urine constitute suitable sources of biological material, while both harboring their specific challenges for analytics concerning low-abundance biomarkers. Dissolved proteins and nucleic acids as well as cells and cell-bound molecules can be the analytes. In urine, exfoliated bladder tumor cells have to be identified and in blood, circulating tumor cells have to be detected among huge amounts of other cells. For the detection of both low-abundance cells and molecules, their specific enrichment prior to analysis is advantageous or even necessary. Adapted methods for the analysis of proteomes and subproteomes by 2D-gel electrophoresis, multidimensional chromatography and antibody arrays are discussed. Analysis of nucleic acid-based markers exploits the high amplification power of PCR and modified PCR combined with previous (subtransciptomes) or subsequent (microarray) enrichment to sensitively and specifically detect markers. DNA mutations, DNA-methylation status and apoptotic DNA fragments, as well as levels of ribonucleic acids including microRNAs, can be analyzed by means of these methods. Finally, the challenge of identifying circulating tumor cells and assigning them to their original tissue is critically discussed. PY - 2010 DO - https://doi.org/10.4155/BIO.09.174 SN - 1757-6180 VL - 2 IS - 2 SP - 295 EP - 309 PB - Future Science Group CY - London AN - OPUS4-20981 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Nießner, R. A1 - Broekaert, J.A. A1 - Bron, M. A1 - Einax, J. A1 - Emons, H. A1 - Haisch, C. A1 - Huber, C. A1 - Jakubowski, Norbert A1 - Knopp, D. A1 - Popp, J. A1 - Schäferling, M. T1 - Analytische Chemie 2008/2009 JF - Nachrichten aus der Chemie PY - 2010 DO - https://doi.org/10.1002/nadc.201063123 SN - 1439-9598 SN - 1521-3854 VL - 58 IS - 3 SP - 223 EP - 235 PB - Wiley-VCH Verl. CY - Weinheim AN - OPUS4-21177 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert T1 - Heritage of Prof. Dr. Ing. Klaus Gustav Heumann: Selected Highlights T2 - 2010 Winter Conference T2 - 2010 Winter Conference CY - Fort Myers, FL, USA DA - 2010-01-04 PY - 2010 AN - OPUS4-21161 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Jakubowski, Norbert A1 - Prohaska, T. A1 - Roos, P.H. ED - Beauchemin, D. ED - Matthews, D. T1 - Polyatomic ions with double-focusing magnet sector mass spectrometers T2 - The encyclopedia of mass spectrometry - elemental and isotope ratio mass spectrometry KW - ICP-MS KW - Sektorfeldgeräte PY - 2010 SN - 978-0-08-043804-7 VL - 5 SP - 132 EP - 150 PB - Elsevier CY - Oxford, UK AN - OPUS4-22237 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Roos, P.H. A1 - Jakubowski, Norbert T1 - Cytochromes - fascinating molecular machines JF - Metallomics N2 - A huge number of functions and reactions can be performed by proteins merely based on their amino acids and their spatial arrangement which results in a specific geometric and electronic environment. The diversity and efficiency of functions, however, can be increased by protein-bound cofactors which fulfil their task in close interaction with the surrounding amino acid side chains. Among these cofactors, metals play an important role. They can be bound to proteins directly via specific amino acids such as cysteine and histidine, for example in zinc finger proteins or in iron sulfur cluster proteins. Another possibility is their integration into the 3D-structure of a protein via a prosthetic group. Porphyrine or porphyrine-like molecules with copper or iron as central bound metal ions constitute important structures in this respect. PY - 2011 DO - https://doi.org/10.1039/c1mt90012a SN - 1756-5901 SN - 1756-591X VL - 3 SP - 316 EP - 318 PB - RSC Publ. CY - Cambridge AN - OPUS4-23830 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert T1 - Neue Anwendungen der anorganischen Massenspektrometrie in den Lebens- und Materialwissenschaften T2 - Eingeladener Vortrag, Fakultätskolloquium, Technische Universität Bergakademie Freiberg T2 - Eingeladener Vortrag, Fakultätskolloquium, Technische Universität Bergakademie Freiberg CY - Freiberg, Germany DA - 2011-02-15 PY - 2011 AN - OPUS4-23809 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Tempez, A. A1 - Chapon, P. A1 - Bordel, N. A1 - Pisonero, J. A1 - Lobo, L. T1 - A new Glow Discharge Time of Fligh Mass Spectrometer for Bulk Analysis and Depth Profiling T2 - PITTCON 2010, eingeladener Vortrag T2 - PITTCON 2010, eingeladener Vortrag CY - Atlanta, GA, USA DA - 2011-03-13 PY - 2011 AN - OPUS4-23810 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Moreno-Gordaliza, E. A1 - Esteban-Fernández, D. A1 - Giesen, Charlotte A1 - Lehmann, K. A1 - Lázaro, A. A1 - Tejedor, A. A1 - Scheler, C. A1 - Canas, B. A1 - Jakubowski, Norbert A1 - Linscheid, M.W. A1 - Gómez-Gómez, M.M. T1 - LA-ICP-MS and nHPLC-ESI-LTQ-FT-MS/MS for the analysis of cisplatin-protein complexes separated by two dimensional gle elctrophoresis in biological samples JF - Journal of analytical atomic spectrometry N2 - A method for the analysis of Pt–protein complexes in biological samples, previously subjected to cisplatin treatment, has been developed. Proteins were separated by gel electrophoresis, and those bound to Pt were detected with high sensitivity by LA-ICP-(SF)-MS. Pt-containing spots were in-gel digested with trypsin, and the peptides produced identified using nHPLC-ESI-LTQ-FT-MS/MS. The influence of protein separation conditions, staining and gel processing prior to laser ablation on Pt–protein bonds preservation have been evaluated using standard proteins incubated with cisplatin. 2-DE separation under non-reducing conditions followed by either Coomassie blue brilliant or silver staining is appropriate for Pt–protein complexes, achieving a good separating resolution of the proteins in biological samples. Direct LA-ICP-MS analysis of glycerol-treated dried gels for Pt–protein monitoring resulted in better sensitivity, more reliable relative Pt signals and a simpler and less time-consuming approach compared to the analysis of blotted membranes. Ablation of gels allowed tackling protein identification of Pt-spots in the remaining non-ablated material in the gel, making it unnecessary to run several gels in parallel for separate Pt detection and protein identification. By using this approach, Pt coordinated to proteins, such as α-2-macroglobulin, transferrin, albumin or hemoglobin, was detected in the serum from a rat treated in vivo with cisplatin after nrSDS-PAGE separation. Furthermore, the first complete LA-ICP-MS metalloprotein contour map in a 2-DE gel has been produced, in this case for the detection of Pt–protein complexes in renal proximal tubule epithelial cells (RPTECs) incubated with cisplatin. Several proteins were identified in those spots containing Pt, which may have a connection with the drug-induced nephrotoxicity mainly affecting this cell type in the kidney. PY - 2012 DO - https://doi.org/10.1039/c2ja30016h SN - 0267-9477 SN - 1364-5544 VL - 27 IS - 9 SP - 1474 EP - 1483 PB - Royal Society of Chemistry CY - London AN - OPUS4-26562 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Moreno-Gordaliza, E. A1 - Esteban-Fernández, D. A1 - Giesen, Ch. A1 - Lehmann, K. A1 - Lázaro-Fernández, A. A1 - Canas, B. A1 - Tejedor, A. A1 - Scheler, Ch. A1 - Linscheid, M. A1 - Gómez-Gómez, M. T1 - LA-ICP-MS for the detection of (semi)metallo-proteins in biological samples after PAGE / 2-DE separations T2 - Vortrag bei Prof. Ogra, University of Pharmaceutical T2 - Vortrag bei Prof. Ogra, University of Pharmaceutical CY - Tokyo, Japan DA - 2013-10-01 PY - 2013 AN - OPUS4-29198 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Moreno-Gordaliza, E. A1 - Esteban-Fernández, D. A1 - Giesen, Ch. A1 - Lehmann, K. A1 - Lázaro-Fernández, A. A1 - Canas, B. A1 - Tejedor, A. A1 - Scheler, Ch. A1 - Linscheid, M. A1 - Gómez-Gómez, M. T1 - LA-ICP-MS for the detection of (semi)metallo-proteins in biological samples after PAGE / 2-DE separations T2 - Vortrag bei Prof. Zuruta, Chuo University T2 - Vortrag bei Prof. Zuruta, Chuo University CY - Tokyo, Japan DA - 2013-10-03 PY - 2013 AN - OPUS4-29201 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert T1 - Development of ICP-MS based methods for label free detection of (semi)metals T2 - Vortrag bei der Discussion Group Plasma Meeting im Institut of Technology T2 - Vortrag bei der Discussion Group Plasma Meeting im Institut of Technology CY - Tokyo, Japan DA - 2013-10-04 PY - 2013 AN - OPUS4-29204 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert T1 - ICP-MS with Sector Field Devices N2 - This lecture is an introduction to ICP-MS with a double focusing magnetic sector mass analyzer. It offers fundamental background, a thorough discussion of analytical features, and state of the art information on applications. Different types of double focusing instruments also are considered. Specific topics include fundamental aspects of ICP-MS (physical properties of a double focusing instrument, operational characteristics in comparison with quadrupole instruments); analytical characteristics (spectral and non-spectral interferences, figures of merit in low and high resolution modes, blanks and memory effects, HPLC and GC interfaces), and applications (industrial including ultra-pure reagents and alloys, environmental, geological, and biomedical materials). T2 - Winter Conference on Plasma Spectrochemistry CY - Tucson, Arizona, USA DA - 10.01.2016 KW - High-resolution ICP-MS KW - Figures of merit KW - Interfaces KW - Applications KW - Ultra-trace analysis PY - 2016 AN - OPUS4-37148 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Büchner, T. A1 - Drescher, D. A1 - Merk, V. A1 - Traub, Heike A1 - Guttmann, P. A1 - Werner, St. A1 - Jakubowski, Norbert A1 - Schneider, G. A1 - Kneipp, J. T1 - Biomolecular environment, quantification, and intracellular interaction of multifunctional magnetic SERS nanoprobes JF - Analyst N2 - Multifunctional composite nanoprobes consisting of iron oxide nanoparticles linked to silver and gold nanoparticles, Ag–Magnetite and Au–Magnetite, respectively, were introduced by endocytic uptake into cultured fibroblast cells. The cells containing the non-toxic nanoprobes were shown to be displaceable in an external magnetic field and can be manipulated in microfluidic channels. The distribution of the composite nanostructures that are contained in the endosomal system is discussed on the basis of surfaceenhanced Raman scattering (SERS) mapping, quantitative laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) micromapping, and cryo soft X-ray tomography (cryo soft-XRT). Cryo soft-XRT of intact, vitrified cells reveals that the composite nanoprobes form intra-endosomal aggregates. The nanoprobes provide SERS signals from the biomolecular composition of their surface in the endosomal environment. The SERS data indicate the high stability of the nanoprobes and of their plasmonic properties in the harsh environment of endosomes and lysosomes. The spectra point at the molecular composition at the surface of the Ag–Magnetite and Au–Magnetite nanostructures that is very similar to that of other Composite structures, but different from the composition of pure silver and gold SERS nanoprobes used for intracellular investigations. As shown by the LA-ICP-MS data, the uptake efficiency of the magnetite composites is approximately two to three times higher than that of the pure gold and silver nanoparticles. KW - Nanoparticles KW - SERS KW - Cell KW - LA-ICP-MS KW - X-ray tomography PY - 2016 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-371811 DO - https://doi.org/10.1039/c6an00890a SN - 0003-2654 VL - 141 IS - 17 SP - 5096 EP - 5106 PB - Royal Society of Chemistry CY - Cambridge, UK AN - OPUS4-37181 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Hsiao, I.-L. A1 - Bierkandt, Frank A1 - Reichardt, Ph. A1 - Luch, A. A1 - Huang, Y.-J. A1 - Jakubowski, Norbert A1 - Tentschert, J. A1 - Haase, A. T1 - Quantification and visualization of cellular uptake of TiO2 and Ag nanoparticles: comparison of different ICP-MS techniques JF - Journal of Nanobiotechnology N2 - Safety assessment of nanoparticles (NPs) requires techniques that are suitable to quantify tissue and cellular uptake of NPs. The most commonly applied techniques for this purpose are based on inductively coupled plasma mass spectrometry (ICP-MS). Here we apply and compare three different ICP-MS methods to investigate the cellular uptake of TiO2 (diameter 7 or 20 nm, respectively) and Ag (diameter 50 or 75 nm, respectively) NPs into differentiated mouse neuroblastoma cells (Neuro-2a cells). Cells were incubated with different amounts of the NPs. Thereafter they were either directly analyzed by laser ablation ICP-MS (LA-ICP-MS) or were lysed and lysates were analyzed by ICP-MS and by single particle ICP-MS (SP-ICP-MS). KW - Nanoparticles KW - Single particle ICP-MS KW - Laser ablation ICP-MS KW - Cellular internalization KW - Neurons PY - 2016 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-371620 DO - https://doi.org/10.1186/s12951-016-0203-z SN - 1477-3155 VL - 14 SP - Article 50 AN - OPUS4-37162 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Moraleja, I. A1 - Esteban-Fernández, Diego A1 - Lázaro, A. A1 - Humanes, B. A1 - Neumann, B. A1 - Tejedor, A. A1 - Mena, M. A1 - Jakubowski, Norbert A1 - Gómez-Gómez, M. T1 - Printing metal-spiked inks for LA-ICP-MS bioimaging internal standardization: comparison of the different nephrotoxic behavior of cisplatin, carboplatin, and oxaliplatin JF - Analytical and Bioanalytical Chemistry - Springer N2 - The study of the distribution of the cytostatic drugs cisplatin, carboplatin, and oxaliplatin along the kidney may help to understand their different nephrotoxic behavior. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) allows the acquisition of trace element images in biological tissues. However, results obtained are affected by several variations concerning the sample matrix and instrumental drifts. In this work, an internal standardization method based on printing an Ir-spiked ink onto the surface of the sample has been developed to evaluate the different distributions and accumulation levels of the aforementioned drugs along the kidney of a rat model. A conventional ink-jet printer was used to print fresh sagittal kidney tissue slices of 4 μm. A reproducible and homogenous deposition of the ink along the tissue was observed. The ink was partially absorbed on top of the tissue. Thus, this approach provides a pseudo-internal standardization, due to the fact that the ablation sample and internal standard take place subsequently and not simultaneously. A satisfactory normalization of LA-ICP-MS bioimages and therefore a reliable comparison of the kidney treated with different Pt-based drugs were achieved even for tissues analyzed on different days. Due to the complete ablation of the sample, the transport of the ablated internal standard and tissue to the inductively coupled plasma-mass spectrometry (ICP-MS) is practically taking place at the same time. Pt accumulation in the kidney was observed in accordance to the dosages administered for each drug. Although the accumulation rate of cisplatin and oxaliplatin is high in both cases, their Pt distributions differ. The strong nephrotoxicity observed for cisplatin and the absence of such side effect in the case of oxaliplatin could explain these distribution differences. The homogeneous distribution of oxaliplatin in the cortical and medullar areas could be related with its higher affinity for cellular transporters such as MATE2-k. KW - Bioimaging KW - LA-ICP-MS KW - Cisplatin KW - Standardization KW - Printed internal standard KW - Pt-based drugs KW - Nephrotoxicity PY - 2016 DO - https://doi.org/10.1007/s00216-016-9327-0 SN - 1618-2642 SN - 1618-2650 VL - 408 IS - 9 SP - 2309 EP - 2318 AN - OPUS4-36110 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Jakubowski, Norbert T1 - Freddy Adams and Carlo Barbante: Chemical imaging analysis (Book review) T2 - Analytical and Bioanalytical Chemistry N2 - In this book many practical hints are given and analytical problems are discussed by the authors from their own experiences. A critical assessment of the methods is given, which is most important for decision makers to select a good combination of complementary methods to solve practical analytical problems. KW - Chemical imaging analysis KW - Surface analysis KW - Interface analysis PY - 2016 DO - https://doi.org/10.1007/s00216-016-9517-9 SN - 1618-2642 SN - 1618-2650 VL - 408 IS - 16 SP - 4199 EP - 4201 PB - Springer AN - OPUS4-36216 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Scharlach, C. A1 - Müller, Larissa A1 - Wagner, S. A1 - Kobayashi, Y. A1 - Kratz, H. A1 - Ebert, M. A1 - Jakubowski, Norbert A1 - Schellenberger, E. T1 - LA-ICP-MS allows quantitative microscopy of europium-doped iron oxide nanoparticles and is a possible alternative to ambiguous Prussian blue iron staining JF - Journal of Biomedical Nanotechnology N2 - The development of iron oxide nanoparticles for biomedical applications requires accurate histological evaluation. Prussian blue iron staining is widely used but may be unspecific when tissues contain substantial endogenous iron. Here we tested whether microscopy by laser ablation coupled to inductively coupled plasma mass spectrometry (LA-ICP-MS) is sensitive enough to analyze accumulation of very small iron oxide particles (VSOP) doped with europium in tissue sections. KW - Atherosclerotic plaques KW - LA-ICP-MS microscopy KW - Quantification KW - Element microscopy KW - Histology PY - 2016 DO - https://doi.org/10.1166/jbn.2016.2230 SN - 1550-7033 SN - 1550-7041 VL - 12 IS - 5 SP - 1001 EP - 1010 AN - OPUS4-36189 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Traub, Heike A1 - Kneipp, J. A1 - Drescher, D. T1 - Entwicklung von Methoden zur quantitativen Analyse von Nanopartikeln in biologischen Proben T2 - BfR-Seminar T2 - BfR-Seminar CY - Berlin, Germany DA - 2014-06-04 PY - 2014 AN - OPUS4-30780 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Munter, L.M. A1 - Sieg, H. A1 - Bethge, T. A1 - Liebsch, F. A1 - Bierkandt, Frank A1 - Schleeger, M. A1 - Bittner, H.J. A1 - Heberle, J. A1 - Jakubowski, Norbert A1 - Hildebrand, P.W. A1 - Multhaup, G. T1 - Model peptides uncover the role of the beta-secretase transmembrane sequence in metal ion mediated oligomerization JF - Journal of the American chemical society N2 - The β-secretase or β-site amyloid precursor protein cleaving enzyme 1 (BACE1) is the enzyme responsible for the formation of amyloid-β peptides, which have a major role in Alzheimer pathogenesis. BACE1 has a transmembrane sequence (TMS), which makes it unique among related proteases. We noticed that the BACE1 TMS contains an uncommon sulfur-rich motif. The sequence MxxxCxxxMxxxCxMxC spans the entire TMS, resembles metal ion binding motifs, and is highly conserved among homologues. We used a synthetic 31-mer model peptide comprising the TMS to study metal ion binding and oligomerization. Applying diverse biochemical and biophysical techniques, we detected dimer and trimer formation of the TMS peptide with copper ions. Replacement of the central Cys466 by Ala essentially abolished these effects. We show that the peptide undergoes a redox reaction with copper ions resulting in a disulfide bridge involving Cys466. Further, we find peptide trimerization that depends on the presence of monovalent copper ions and the sulfhydryl group of Cys466. We identified Cys466 as a key residue for metal ion chelation and to be the core of an oligomerization motif of the BACE1-TMS peptide. Our results demonstrate a novel metal ion controlled oligomerization of the BACE1 TMS, which could have an enormous therapeutic importance against Alzheimer disease. KW - Enzymes KW - Amyloid formation KW - Alzheimer disease KW - Copper PY - 2013 DO - https://doi.org/10.1021/ja410812r SN - 0002-7863 SN - 1520-5126 VL - 135 IS - 51 SP - 19354 EP - 19361 PB - American Chemical Society CY - Washington, DC AN - OPUS4-30543 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Hösl, Simone A1 - Müller, Larissa T1 - Development of Immuno-assays for LA-ICP-MS detection T2 - Winter Conference on Plasma Spectrochemistry 2014 T2 - Winter Conference on Plasma Spectrochemistry 2014 CY - Amelia Island, FL, USA DA - 2014-01-05 PY - 2014 AN - OPUS4-30246 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert T1 - Short Course: ICP-MS with Sector Field Devices T2 - Winter Conference on Plasma Spectrochemistry 2014 T2 - Winter Conference on Plasma Spectrochemistry 2014 CY - Amelia Island, FL, USA DA - 2014-01-05 PY - 2014 AN - OPUS4-30247 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Hösl, Simone A1 - Neumann, B. A1 - Techritz, Sandra A1 - Linscheid, M. A1 - Theuring, F. A1 - Scheler, C. A1 - Jakubowski, Norbert A1 - Müller, Larissa T1 - Development of a calibration and standardization procedure for LA-ICP-MS using a conventional ink-jet printer for quantification of proteins in electro- and western-blot assays JF - Journal of analytical atomic spectrometry N2 - We developed new procedures for internal standardization and calibration to be used for laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) for elemental micro mapping imaging of biological samples like Western blot membranes and tissue sections. These procedures are based on printing of metal spiked inks onto the top of thin layer samples for simultaneous internal standardization and calibration of LA-ICP-MS. In the case of internal standardization the ink is spiked with indium as an internal standard and homogenously printed over the entire membrane (size 56 cm2) prior to LA-ICP-MS detection, a standard deviation (RSD) value of 2% was achieved. In the second approach the metal content of lanthanide tagged proteins and antibodies after biological work flows was quantified by LA-ICP-MS on nitro-cellulose membranes. In this case the inks spiked with varying metals were printed with different densities on the same nitrocellulose membranes in well-defined squares to produce matrix-matched calibration standards. For validation and calibration the ink squares were excised and the specific metal content was measured by liquid ICP-MS after solubilization of the membrane slice. For the printed calibration standard limits of detection (LOD) of <4 fmol for different metals and relative process standard deviations of 1–2% only were determined via LA-ICP-MS. PY - 2014 DO - https://doi.org/10.1039/c4ja00060a SN - 0267-9477 SN - 1364-5544 VL - 29 IS - 7 SP - 1282 EP - 1291 PB - Royal Society of Chemistry CY - London AN - OPUS4-31171 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Bierkandt, Frank A1 - Haase, A. A1 - Jungnickel, H. A1 - Tentschert, J. A1 - Luch, A. A1 - Drescher, Daniela A1 - Traub, Heike A1 - Kneipp, Janina T1 - Quantitative analysis of cell cultures treated with metallic nanoparticles using laser ablation ICP-MS T2 - SCIX 2014 T2 - SCIX 2014 CY - Reno, NV, USA DA - 2014-09-28 PY - 2014 AN - OPUS4-31896 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Drescher, Daniela A1 - Zeise, Ingrid A1 - Traub, Heike A1 - Guttmann, P. A1 - Seifert, Stephan A1 - Büchner, Tina A1 - Jakubowski, Norbert A1 - Schneider, G. A1 - Kneipp, Janina T1 - In situ characterization of SiO2 nanoparticle biointeractions using BrightSilica JF - Advanced functional materials N2 - By adding a gold core to silica nanoparticles (BrightSilica), silica-like nanoparticles are generated that, unlike unmodified silica nanoparticles, provide three types of complementary information to investigate the silica nano-biointeraction inside eukaryotic cells in situ. Firstly, organic molecules in proximity of and penetrating into the silica shell in live cells are monitored by surface-enhanced Raman scattering (SERS). The SERS data show interaction of the hybrid silica particles with tyrosine, cysteine and phenylalanine side chains of adsorbed proteins. Composition of the biomolecular corona of BrightSilica nanoparticles differs in fibroblast and macrophage cells. Secondly, quantification of the BrightSilica nanoparticles using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) micromapping indicates a different interaction of silica nanoparticles compared to gold nanoparticles under the same experimental conditions. Thirdly, the metal cores allow the investigation of particle distribution and interaction in the cellular ultrastructure by cryo nanoscale X-ray tomography (cryo-XT). In 3D reconstructions the assumption is confirmed that BrightSilica nanoparticles enter cells by an endocytotic mechanism. The high SERS intensities are explained by the beneficial plasmonic properties due to agglomeration of BrightSilica. The results have implications for the development of multi-modal qualitative and quantitative characterization in comparative nanotoxicology and bionanotechnology. KW - Silica nanoparticles KW - Surface-enhanced Raman scattering KW - X-ray tomography KW - LA-ICP-MS KW - Core–shell structures PY - 2014 DO - https://doi.org/10.1002/adfm.201304126 SN - 1616-301X SN - 1616-3028 VL - 24 IS - 24 SP - 3765 EP - 3775 PB - Wiley-VCH CY - Weinheim AN - OPUS4-30924 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Müller, Larissa A1 - Traub, Heike A1 - Jakubowski, Norbert A1 - Drescher, Daniela A1 - Baranov, Vladimir A1 - Kneipp, Janina T1 - Trends in single-cell analysis by use of ICP-MS JF - Analytical and bioanalytical chemistry N2 - The analysis of single cells is a growing research field in many disciplines such as toxicology, medical diagnosis, drug and cancer research or metallomics, and different methods based on microscopic, mass spectrometric, and spectroscopic techniques are under investigation. This review focuses on the most recent trends in which inductively coupled plasma mass spectrometry (ICP-MS) and ICP optical emission spectrometry (ICP-OES) are applied for single-cell analysis using metal atoms being intrinsically present in cells, taken up by cells (e.g., nanoparticles), or which are artificially bound to a cell. For the latter, especially element tagged antibodies are of high interest and are discussed in the review. The application of different sample introduction systems for liquid analysis (pneumatic nebulization, droplet generation) and elemental imaging by laser ablation ICP-MS (LA-ICP-MS) of single cells are highlighted. Because of the high complexity of biological systems and for a better understanding of processes and dynamics of biologically or medically relevant cells, the authors discuss the idea of 'multimodal spectroscopies.' KW - Bioanalytical methods KW - Cell systems/single cell analysis KW - Mass spectrometry/ICP-MS PY - 2014 DO - https://doi.org/10.1007/s00216-014-8143-7 SN - 1618-2642 SN - 1618-2650 VL - 406 IS - 27 SP - 6963 EP - 6977 PB - Springer CY - Berlin AN - OPUS4-31717 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Büchner, Tina A1 - Drescher, Daniela A1 - Traub, Heike A1 - Schrade, P. A1 - Bachmann, S. A1 - Jakubowski, Norbert A1 - Kneipp, Janina T1 - Relating surface-enhanced Raman scattering signals of cells to gold nanoparticle aggregation as determined by LA-ICP-MS micromapping JF - Analytical and bioanalytical chemistry N2 - The cellular response to nanoparticle exposure is essential in various contexts, especially in nanotoxicity and nanomedicine. Here, 14-nm gold nanoparticles in 3T3 fibroblast cells are investigated in a series of pulse-chase experiments with a 30-min incubation pulse and chase times ranging from 15 min to 48 h. The gold nanoparticles and their aggregates are quantified inside the cellular ultrastructure by laser ablation inductively coupled plasma mass spectrometry micromapping and evaluated regarding the surface-enhanced Raman scattering (SERS) signals. In this way, both information about their localization at the micrometre scale and their molecular nanoenvironment, respectively, is obtained and can be related. Thus, the nanoparticle pathway from endocytotic uptake, intracellular processing, to cell division can be followed. It is shown that the ability of the intracellular nanoparticles and their accumulations and aggregates to support high SERS signals is neither directly related to nanoparticle amount nor to high local nanoparticle densities. The SERS data indicate that aggregate geometry and interparticle distances in the cell must change in the course of endosomal maturation and play a critical role for a specific gold nanoparticle type in order to act as efficient SERS nanoprobe. This finding is supported by TEM images, showing only a minor portion of aggregates that present small interparticle spacing. The SERS spectra obtained after different chase times show a changing composition and/or structure of the biomolecule corona of the gold nanoparticles as a consequence of endosomal processing. KW - Gold nanoparticles KW - Surface-enhanced Raman scattering KW - LA-ICP-MS KW - Fibroblast KW - Cell KW - Particle aggregation KW - Endosome PY - 2014 DO - https://doi.org/10.1007/s00216-014-8069-0 SN - 1618-2642 SN - 1618-2650 VL - 406 IS - 27 SP - 7003 EP - 7014 PB - Springer CY - Berlin AN - OPUS4-31718 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Roos, P. H. A1 - Wäntig, L. T1 - Bio-Elemental Imaging by Use of Laser Ablation Inductively Coupled Plasma Mass Sepctrometry, Workshop: Elemental Mass Spectrometry and Proteonomics on Clinical and Biological Samples T2 - 18th International Mass Spectrometry Conference T2 - 18th International Mass Spectrometry Conference CY - Bremen, Germany DA - 2009-08-30 PY - 2009 AN - OPUS4-19852 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Roos, P. H. A1 - Wäntig, L. T1 - Elemental Mass Spectrometry: Metallomics and Beyond, Workshop 3: Elemental Mass Spectrometry and Proteomics on Clinical and Biological Samples T2 - 18th International Mass Spectrometry Conference T2 - 18th International Mass Spectrometry Conference CY - Bremen, Germany DA - 2009-08-30 PY - 2009 AN - OPUS4-19853 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Wäntig, L. A1 - Roos, P. H. T1 - Imaging by laser ablation ICP-SFMS: strength and weakness T2 - 8th International Sector Field ICP-MS Conference T2 - 8th International Sector Field ICP-MS Conference CY - Ghent, Belgium DA - 2009-09-14 PY - 2009 AN - OPUS4-19854 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Müller, Larissa A1 - Roos, P. H. T1 - ICP-MS eine neue Methode für die Bioanalytik? T2 - Johannes Gutenberg-Universität Mainz, GRK-Seminar "Elementspeziation" T2 - Johannes Gutenberg-Universität Mainz, GRK-Seminar "Elementspeziation" CY - Mainz, Germany DA - 2010-06-15 PY - 2010 N1 - Geburtsname von Müller, Larissa: Wäntig, L. - Birth name of Müller, Larissa: Wäntig, L. AN - OPUS4-21990 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert T1 - Elementmassenspektrometrie und Metallomics als neue Herausforderung für die Lebenswissenschaften T2 - Kolloquium, Forschungszentrum Jülich T2 - Kolloquium, Forschungszentrum Jülich CY - Jülich, Germany DA - 2009-12-09 PY - 2009 AN - OPUS4-20378 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Müller, Larissa A1 - Jakubowski, Norbert A1 - Hayen, H. A1 - Roos, P.H. T1 - Iodination of proteins, proteomes and antibodies with potassium triodide for LA-ICP-MS based proteomic analyses JF - Journal of analytical atomic spectrometry N2 - A fast and mild method for iodine labelling of proteins is presented which is specifically designed and optimized for laser ablation (LA-)ICP-MS based proteomics. Single proteins (lysozyme, bovine serum albumin, cytochrome c and β-casein), whole proteomes (microsomal proteome of rats) and antibodies (anti-bovine casein, anti-bovine serum albumin) can be efficiently iodinated by means of potassium triiodide with minimal losses of antigen properties and antibody binding to iodinated proteins. A comparison with iodination by use of IODO-Beads is presented and it is shown that triiodide labelling is a fast, cheap and less laborious alternative without compromising the analytical figures of merit. PY - 2011 DO - https://doi.org/10.1039/c1ja10090d SN - 0267-9477 SN - 1364-5544 N1 - Geburtsname von Müller, Larissa: Wäntig, L. - Birth name of Müller, Larissa: Wäntig, L. VL - 26 SP - 1610 EP - 1618 PB - Royal Society of Chemistry CY - London AN - OPUS4-24209 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Müller, Larissa A1 - Jakubowski, Norbert A1 - Roos, P.H. T1 - Multi-parametric analysis of cytochrome P450 expression in rat liver microsomes by LA-ICP-MS JF - Journal of analytical atomic spectrometry N2 - Quantitative analysis of cytochrome P450 (CYP) patterns of cells and tissues is an important aspect in toxicological and pharmacological research as this group of enzymes is largely involved in the metabolism of toxic compounds and drugs. Here we present a method for the multi-parametric and simultaneous quantitative determination of several cytochromes P450 in liver microsomes of untreated and inducer treated rats. The method is based on the binding of specifically lanthanide labelled antibodies to electrophoretically separated and blotted CYP proteins and their subsequent identification and quantification by LA-ICP-MS. CYP1A1, CYP2B1, CYP2C11, CYP2E1 and CYP3A1 were simultaneously quantified and the patterns between microsomal samples were compared. Microsomes of rats treated with 3-methylcholanthrene, phenobarbital and dexamethasone showed increased levels of CYP1A1, CYP2B1 and CYP3A1, respectively. These results coincide with data obtained by independent methods for CYP quantification, i.e. ethoxyresorufin O-deethylase activity for CYP1A1 and pentoxyresorufin O-depentylase for CYP2B1. The presented method is useful for multi-parametric CYP profiling and has further large potential with respect to the number of analysed parameters/proteins and sensitivity. PY - 2011 DO - https://doi.org/10.1039/c0ja00077a SN - 0267-9477 SN - 1364-5544 N1 - Geburtsname von Müller, Larissa: Wäntig, L. - Birth name of Müller, Larissa: Wäntig, L. VL - 26 IS - 2 SP - 310 EP - 319 PB - Royal Society of Chemistry CY - London AN - OPUS4-23465 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Jakubowski, Norbert A1 - Prohaska, T. A1 - Rottmann, L. A1 - Vanhaecke, F. T1 - Inductively coupled plasma- and glow discharge plasma-sector field mass spectrometry - Part I. Tutorial: Fundamentals and instrumentation JF - Journal of analytical atomic spectrometry N2 - The aim of this series of two reviews is to introduce the basic concepts of ICP and GD sector field instruments, to discuss their peculiarities and performance, to present selected analytical applications for demonstration of the 'state of the art' and, finally, to identify possible future trends and developments. Part I focuses on fundamentals, instrumentation and operation of instruments to give an overview of the capabilities of the actual commercially available instrumentation, whereas selected applications will be discussed in detail in part II. PY - 2011 DO - https://doi.org/10.1039/c0ja00161a SN - 0267-9477 SN - 1364-5544 VL - 26 IS - 4 SP - 693 EP - 726 PB - Royal Society of Chemistry CY - London AN - OPUS4-23466 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Jakubowski, Norbert T1 - Inductively coupled plasma- and glow discharge plasma-sector field mass spectrometry - Part II. Applications JF - Journal of analytical atomic spectrometry N2 - Part I of this series of two reviews focused on fundamentals, instrumentation and operation of sector field instruments to give a proper overview of the capabilities of the actual commercially available instrumentation. In part II, selected applications of the last decade are discussed in detail concluding with pinpointing possible future trends and current developments. KW - ICP KW - SF KW - MS PY - 2011 DO - https://doi.org/10.1039/c0ja00007h SN - 0267-9477 SN - 1364-5544 VL - 26 IS - 4 SP - 727 EP - 757 PB - Royal Society of Chemistry CY - London AN - OPUS4-23467 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert T1 - Combination of imaging by laser ablation ICP-MS and detection of element-tagged antibodies for application in Western Blot assays T2 - BCEIA Conference T2 - BCEIA Conference CY - Beijing, China DA - 2009-11-25 PY - 2009 AN - OPUS4-20585 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Pedrero, Z. A1 - Murillo, S. A1 - Cámara, C. A1 - Schram, E. A1 - Luten, J.B. A1 - Feldmann, I. A1 - Jakubowski, Norbert A1 - Madrid, Y. T1 - Selenium speciation in different organs of African catfish (Clarias gariepinus) enriched through a selenium-enriched garlic based diet JF - Journal of analytical atomic spectrometry N2 - Speciation of Se in fish is needed to elucidate the metabolism of this element in living organisms in the marine environment. In this paper, selenium concentration and its species distribution in several organs and tissues (liver, gills, kidney, muscle and gastrointestinal tract) of African catfish fed with a selenium-enriched garlic based diet was studied. The intention of this paper is focused on both the investigation of selenium distribution in the soluble protein fraction and the detection of selenoaminoacids. Thus, two different procedures have been developed. In the first procedure, screening of selenium in proteins in the Tris-buffer soluble fraction of different tissues was carried out by size exclusion chromatography-inductively coupled plasma-mass spectrometry (SEC-ICP-MS) and by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separation and electroblotting onto membranes. For the amino acid analysis, several sample treatments for Se-species extraction, based on enzymatic hydrolysis, were compared. The best results were obtained for incubation at controlled temperature. Application of several sample treatments in conjunction with different chromatographic techniques (reverse phase, anion exchange and ion exchange/size exclusion) was crucial to unambiguous Se-species identification. In Se-enriched African catfish a noticeable increase in the content of selenium in different organs was observed, except for the liver, where the Se level remained unaltered. The kidney was the Se-target organ in animals fed with enriched Se food. Selenomethionine (SeMet) was the main Se species identified in fillet extracts, whereas the presence of selenocysteine (SeCys) was detected in the liver and both SeMet and SeCys were present in the kidney. PY - 2011 DO - https://doi.org/10.1039/c003889j SN - 0267-9477 SN - 1364-5544 VL - 26 IS - 1 SP - 116 EP - 125 PB - Royal Society of Chemistry CY - London AN - OPUS4-22981 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert A1 - Traub, Heike A1 - Kneipp, Janina A1 - Drescher, Daniela T1 - ICP-MS based methods for the quantitative analysis of nanoparticles in biological samples T2 - 5th International Symposium on Metallomics 2015 T2 - 5th International Symposium on Metallomics 2015 CY - Beijing, China DA - 2015-09-09 PY - 2015 AN - OPUS4-34524 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -