TY - CONF A1 - Herrmann, Antje Jutta A1 - Techritz, Sandra A1 - Panne, Ulrich A1 - Jakubowski, Norbert A1 - Haase, A. A1 - Müller, Larissa T1 - Simple and fast metal staining procedures for identification and characterization of single cells via laser ablation ICP-MS N2 - Biological systems exhibit a very high complexity, because they consist of various cell populations showing heterogenic characteristics. Therefore the individual analysis of single cells is important to understand cellular processes as well as their function in a cell system. Especially investigation in the question about what is biological variability and what is a substantial difference between two cells of the same type (which might lead to dysfunction or disease) is of high interest. Today improved spatial and temporal resolution enable the use of laser ablation inductively coupled mass spectrometry (LA-ICP-MS) for element microscopy of single cells. Next to the characterization of natural trace elements the introduction of artificial metal labels into cells is of high interest. Element labeling of cell compartments and/or proteins allows its simultaneous analysis and localization within a cell via element microscopy. Two fast and simple metal staining procedures of adherent cells for identification of single cells via LA-ICP-MS at sub micrometer resolution are presented. Two labeling reagents were selected which were already described in literature for applications around protein and cell analysis. An Iridium-intercalator is utilized to stain the cell nuclei whereas the whole cell is stained by maleimido-mono-amide-DOTA-complexes (mDOTA) loaded with lanthanide(III) isotope ions. The metal staining procedures allow the visualization of single cells by element microscopy independent of a superposition of analyte’s 2D element intensity profile with a prior taken bright field image of the sample. The applicability was shown on an actual question in nano-toxicology (the cellular uptake of nanoparticles). T2 - 25. ICP-MS Anwendertreffen CY - Siegen, Germany DA - 12.09.2016 KW - Metal staining procedures KW - Single cell analysis KW - Bioimaging PY - 2016 AN - OPUS4-37979 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Herrmann, Antje A1 - Müller, Larissa A1 - Techritz, Sandra A1 - Jakubowski, Norbert A1 - Haase, A. A1 - Panne, Ulrich T1 - Simple and fast metal staining procedures for identification of single cells via Laser Ablation ICP-MS N2 - Biological systems exhibit a very high complexity, because they consist of various cell populations showing heterogenic characteristics. Therefore the individual analysis of single cells is important to understand cellular processes as well as their function in a cell system. Especially investigation in the question about what is biological variability and what is a substantial difference between two cells of the same type (which might lead to dysfunction or disease) is of high interest. Today improved spatial and temporal resolution enable the use of laser ablation inductively coupled mass spectrometry (LA-ICP-MS) for element microscopy of single cells. Next to the characterization of natural trace elements the introduction of artificial metal labels into cells is of high interest. Element labeling of cell compartments and/or proteins allows its simultaneous analysis and localization within a cell via element microscopy. Two fast and simple metal staining procedures of adherent cells for identification of single cells via LA-ICP-MS at sub micrometer resolution are presented. An Iridium-intercalator is utilized to stain the cell nuclei whereas the whole cell is stained by maleimido-mono-amide-DOTA-complexes (mDOTA) loaded with lanthanide(III) isotope ions. The metal staining procedures allow the visualization of single cells by element microscopy independent of a superposition of analyte’s 2D element intensity profile with a prior taken bright field image of the sample. T2 - EWLA 2016 CY - Ljubljana, Slovenia DA - 12.07.2016 KW - bioimaging KW - single cell analysis PY - 2016 AN - OPUS4-37077 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -