TY - JOUR A1 - Giesen, Charlotte A1 - Jakubowski, Norbert A1 - Panne, Ulrich A1 - Weller, Michael G. T1 - Ochratoxin-A-Bestimmung in Wein mittels eines ICP-MS-basierten Immunoassays PY - 2010 UR - http://www.analytik-news.de/Fachartikel/Volltext/BAM2.pdf SP - 1 EP - 6 PB - Dr. Beyer Internet-Beratung CY - Ober-Ramstadt AN - OPUS4-22702 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Giesen, Charlotte A1 - Jakubowski, Norbert A1 - Panne, Ulrich A1 - Weller, Michael G. T1 - Comparison of ICP-MS and photometric detection of an immunoassay for the determination of ochratoxin A in wine N2 - An immunoassay was developed for ochratoxin A (OTA) detection in wine and two completely different detection methods were compared: ICP-MS and photometry. For labelling, we applied secondary antibodies conjugated with gold nanoparticles for ICP-MS and with horseradish peroxidase for photometric detection, respectively. Detection limits of the assay were determined as 0.003 µg L-1 for both detection methods. OTA in wine was determined below the EU limit value of 2 µg kg-1. The measurement range was between 0.01 and 1 µg L-1 for ICP-MS and photometric detection. Assay precision detected by ICP-MS and photometry were similar showing that precision of the detection method has only a minor effect on total assay precision. By using BSA as a buffer additive to minimize nonspecific binding, we could also confirm that OTA strongly binds to BSA. In a comparison study of several additives, casein was found to be preferable. PY - 2010 U6 - https://doi.org/10.1039/c0ja00009d SN - 0267-9477 SN - 1364-5544 VL - 25 IS - 10 SP - 1567 EP - 1572 PB - Royal Society of Chemistry CY - London AN - OPUS4-22704 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Giesen, Charlotte A1 - Weller, Michael G. A1 - Jakubowski, Norbert A1 - Panne, Ulrich T1 - Determination of Ochratoxins in wine by an ICP-MS-based Enzyme-Linked Immunosorbent Assay T2 - GDMS-Jahrestagung CY - Halle (Saale), Germany DA - 2010-03-07 PY - 2010 AN - OPUS4-20818 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Giesen, Charlotte A1 - Jakubowski, Norbert A1 - Panne, Ulrich A1 - Weller, Michael G. T1 - Comparison of ICP-MS and Photometric Detection of an Immunoassay for the Determination of Ochratoxin A in Wine T2 - Laser Ablation Workshop CY - Kiel, Germany DA - 2010-06-29 PY - 2010 AN - OPUS4-22585 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Giesen, Charlotte A1 - Panne, Ulrich A1 - Weller, Michael G. T1 - Application of labelled Antibodies for ICP-MS Detection T2 - FACSS 2010 CY - Raleigh, NC, USA DA - 2010-10-17 PY - 2010 AN - OPUS4-22586 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Giesen, Charlotte A1 - Panne, Ulrich A1 - Jakubowski, Norbert A1 - Weller, Michael G. T1 - ICP-MS-Detektion eines Immunoassys zur Bestimmung von Ochratoxin A in Wine T2 - ICP-MS Anwendertreffen CY - Berlin, Germany DA - 2010-09-06 PY - 2010 AN - OPUS4-22588 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -