TY - CONF A1 - Gabriel, Stefan A1 - Weidner, Steffen A1 - Panne, Ulrich A1 - Schwarzinger, C. T1 - Seperation effects caused by dried-droplet sample preparation in MALDI mass spectrometry of polymers T2 - MACRO BeGE 2012 CY - Houffalize, Belgium DA - 2012-12-03 PY - 2012 AN - OPUS4-27603 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gabriel, Stefan A1 - Weidner, Steffen A1 - Panne, Ulrich A1 - Schwarzinger, C. T1 - Separationseffekte in "dried-droplet" Probenpräparationen für MALDI MS von synthetischen Polymeren T2 - Bruker Anwendertreffen CY - Berlin, Germany DA - 2013-02-26 PY - 2013 AN - OPUS4-29253 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gabriel, Stefan A1 - Weidner, Steffen A1 - Panne, Ulrich A1 - Schwarzinger, C. T1 - Separation effects in dried-droplet sample preparations for MALDI mass spectrometry of polymers T2 - Jahrestagung der DGMS CY - Berlin, Germany DA - 2013-03-10 PY - 2013 AN - OPUS4-29257 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gabriel, Stefan A1 - Weidner, Steffen A1 - Panne, Ulrich A1 - Schwarzinger, C. T1 - Separation effects in dried-droplet sample preparations for MALDI mass spectrometry of polymers T2 - Anakon 2013 CY - Essen, Germany DA - 2013-03-04 PY - 2013 AN - OPUS4-29254 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gabriel, Stefan A1 - Weidner, Steffen A1 - Panne, Ulrich A1 - Schwarzinger, C. T1 - Separation effects caused by the dried-droplet sample preparation for MALDI-MS of synthetic polymers T2 - ASMS Conference on Mass Spectrometry and Allied Topics CY - Minneapolis, MN, USA DA - 2013-06-08 PY - 2013 AN - OPUS4-29252 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gabriel, Stefan A1 - Weidner, Steffen A1 - Panne, Ulrich A1 - Schwarzinger, C. T1 - Separation effects caused by the dried dropled sample preparation for MALDI-MS of synthetic polymers T2 - Adlershofer Forschungsforum 2013 CY - Berlin, Germany DA - 2013-11-12 PY - 2013 AN - OPUS4-30163 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gabriel, Stefan A1 - Mishra, Kirti Bhushan A1 - Weidner, Steffen T1 - Molmassenabhängige Verteilung einer Dried Droplet Probe in Abhängigkeit von Lösungsmitteln und Polymeren T2 - 16. Kolloquium MALDI und Synthetische Polymere CY - Berlin, Germany DA - 2012-05-08 PY - 2012 AN - OPUS4-26136 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Gabriel, Stefan A1 - Pfeifer, Dietmar A1 - Schwarzinger, C. A1 - Panne, Ulrich A1 - Weidner, Steffen T1 - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric imaging of synthetic polymer sample spots prepared using ionic liquid matrices N2 - RATIONALE Polymer sample spots for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) prepared by the dried-droplet method often reveal ring formation accompanied by possible segregation of matrix and sample molecules as well as of the polymer homologs itself. Since the majority of sample spots are prepared by this simple and fast method, a matrix or sample preparation method that excludes such segregation has to be found. METHODS Three different ionic liquid matrices based on conventionally used aromatic compounds for MALDI-TOF MS were prepared. The formation of ionic liquids was proven by 1H NMR spectroscopy. MALDI-Imaging mass spectrometry was applied to monitor the homogeneity. RESULTS Our results show a superior sample spot homogeneity using ionic liquid matrices. Spots could be sampled several times without visible differences in the mass spectra. A frequently observed loss of matrix in the mass spectrometer vacuum was not observed. The necessary laser irradiance was reduced, which resulted in less polymer fragmentation. CONCLUSIONS Ionic liquid matrices can be used to overcome segregation, a typical drawback of conventional MALDI dried-droplet preparations. Homogeneous sample spots are easy to prepare, stable in the MS vacuum and, thereby, improve the reproducibility of MALDI. PY - 2014 U6 - https://doi.org/10.1002/rcm.6810 SN - 0951-4198 SN - 1097-0231 VL - 28 IS - 5 SP - 489 EP - 498 PB - Wiley CY - Chichester AN - OPUS4-30267 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Gabriel, Stefan A1 - Schwarzinger, C. A1 - Schwarzinger, B. A1 - Panne, Ulrich A1 - Weidner, Steffen T1 - Matrix segregation as the major cause for sample inhomogeneity in MALDI dried droplet spots N2 - The segregation in dried droplet MALDI sample spots was analyzed with regard to the matrix-to-sample ratio using optical microscopy, MALDI imaging mass spectrometry (MALDI MSI) and IR imaging spectroscopy. In this context, different polymer/matrix/solvent systems usually applied in the analysis of synthetic polymers were investigated. The use of typical matrix concentrations (10 mg mL-1) in almost every case resulted in ring patterns, whereas higher concentrated matrix solutions always led to homogeneous sample spot layers. The data revealed that segregation is predominantly caused by matrix transport in the drying droplet, whereas polymer segregation seems to be only secondary. KW - MALDI KW - Imaging MS KW - Matrix segregation KW - Dried droplet KW - Sample preparation KW - Polymers PY - 2014 U6 - https://doi.org/10.1007/s13361-014-0913-0 SN - 1044-0305 VL - 25 IS - 8 SP - 1356 EP - 1363 PB - American Society for Mass Spectrometry CY - New York, NY AN - OPUS4-32582 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gabriel, Stefan A1 - Weidner, Steffen A1 - Panne, Ulrich A1 - Schwarzinger, C. T1 - MALDI-ToF-MS Imaging: Classical Dried Droplet vs. Ionic Matrix T2 - Advances in Polymer Science and Technology (APST) 3 CY - Linz, Austria DA - 2013-09-09 PY - 2013 AN - OPUS4-30165 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -