TY - JOUR A1 - Gärtner, Stefanie A1 - Carvalho, J.J. A1 - Emmerling, Franziska A1 - Garbe, L.-A. A1 - Schneider, Rudolf T1 - Polyclonal murine and rabbit antibodies for the bile acid isolithocholic acid N2 - Bile acids are relevant markers for clinical research. This study reports the production of antibodies for isolithocholic acid, the isomer of the extensively studied lithocholic acid. The IgG titer and affinity maturation were monitored during the immunizations of three mice and two rabbits. In both animal models, polyclonal antibodies with a high selectivity and affinity were produced. The development of a direct competitive ELISA with a test midpoint of 0.69 ± 0.05 µg/L and a measurement range from 0.09–15 µg/L is reported. Additionally, the crystal structure of isolithocholic acid is described for the first time. KW - ELISA KW - Crystal structure KW - Competitive assay KW - Immunization monitoring KW - 3ß-hydroxy-5ß-cholanic acid PY - 2015 U6 - https://doi.org/10.1080/15321819.2014.924419 SN - 1532-1819 SN - 1532-4230 VL - 36 IS - 3 SP - 233 EP - 252 PB - Taylor & Francis CY - Philadelphia, Pa. AN - OPUS4-31836 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -