TY - JOUR A1 - Symietz, Christian A1 - Lehmann, Erhard A1 - Gildenhaar, Renate A1 - Krüger, Jörg A1 - Berger, Georg T1 - Femtosecond laser induced fixation of calcium alkali phosphate ceramics on titanium alloy bone implant material N2 - Femtosecond lasers provide a novel method of attaching bioceramic material to a titanium alloy, thereby improving the quality of bone implants. The ultrashort 30 fs laser pulses (790 nm wavelength) penetrate a thin dip-coated layer of fine ceramic powder, while simultaneously melting a surface layer of the underlying metal. The specific adjustment of the laser parameters (pulse energy and number of pulses per spot) avoids unnecessary melting of the bioactive calcium phosphate, and permits a defined thin surface melting of the metal, which in turn is not heated throughout, and therefore maintains its mechanical stability. It is essential to choose laser energy densities that correspond to the interval between the ablation fluences of both materials involved: about 0.1-0.4 J cm-2. In this work, we present the first results of this unusual technique, including laser ablation studies, scanning electron microscopy and optical microscope images, combined with EDX data. KW - Bone implant KW - Bioceramic coating KW - Titanium KW - Calcium phosphate KW - Femtosecond laser PY - 2010 DO - https://doi.org/10.1016/j.actbio.2010.02.016 SN - 1742-7061 VL - 6 IS - 8 SP - 3318 EP - 3324 PB - Elsevier CY - Amsterdam AN - OPUS4-21446 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Winkler, T. A1 - Hoenig, E. A1 - Huber, G. A1 - Janssen, R. A1 - Fritsch, D. A1 - Gildenhaar, Renate A1 - Berger, Georg A1 - Morlock, M.M. A1 - Schilling, A.F. T1 - Osteoclastic bioresorption of biomaterials: Two- and three-dimensional imaging and quantification N2 - Purpose: Bioresorbable materials have been developed in the hope that the body will replace them with newly formed tissue. The first step of this remodeling process in bone is the bioresorption of the material by osteoclasts. The aim of this study was to analyze osteoclastic resorption of biomaterials in vitro using the commonly used two-dimensional methods of light-microscopy (LM) and scanning electron microscopy (SEM) in comparison with infinite focus microscopy (IFM), a recently developed imaging method allowing for three-dimensional surface analysis. Methods: Human hematopoietic stem cells were cultivated in the presence of the cytokines M-CSF and RANK-L for 4 weeks directly on dentin and a calcium phosphate cement. Osteoclast development was surveyed with standard techniques. After removal of the cells, resorption was characterized and quantified by LM, SEM and IFM. Results: Osteoclast cultures on the biomaterials presented the typical osteoclast-specific markers. On dentin samples LM, SEM as well as IFM allowed for discrimination of resorption. Quantification of the resorbed area showed a linear correlation between the results (LM vs. SEM: r=0.996, p=0.004; SEM vs. IFM: r=0.989, p=0.011; IFM vs. LM: r=0.995). It was not possible to demarcate resorption pits on GB14 using LM or SEM. With IFM, resorption on GB14 could be visualized and quantified two- and three-dimensionally. KW - Biomaterial KW - Three-dimensional KW - IFM KW - Osteoclast KW - Dentin KW - Calcium phosphate PY - 2010 SN - 0391-3988 VL - 33 IS - 4 SP - 198 EP - 203 PB - Wichtig Ed. CY - Milano [u.a.] AN - OPUS4-21621 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Müller-Mai, C.M. A1 - Berger, Georg A1 - Stiller, M. A1 - Gildenhaar, Renate A1 - Jörn, Daniela A1 - Ploska, Ute A1 - Houshmand, A. A1 - Bednarek, A. A1 - Koch, C. A1 - Knabe, C. T1 - Evaluation of degradable bone cements for percutaneous augmentation of bone defects KW - Bone defect KW - Bioactive bone cement KW - Calcium alkali orthophosphates KW - Histology KW - Histomorphometry KW - Knochendefekt KW - Bioaktiver Knochenzement KW - Calciumalkaliorthophosphat KW - Histologie KW - Histomorphometrie PY - 2010 DO - https://doi.org/10.1002/mawe.201000706 SN - 0933-5137 SN - 1521-4052 VL - 41 IS - 12 SP - 1040 EP - 1047 PB - Wiley-VCH Verl. CY - Weinheim AN - OPUS4-22891 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Berger, Georg T1 - Offen porige Knochen-ersatzwerkstoffe auf Basis von Calcium-Alkali-Phosphaten T2 - 4. Workshop "Biomaterialien" des AK 15 (Mineralogische und Technische Kristallo-graphie) der Dt. Ges. f. Kristallographie (DGK) CY - Halle (Saale), Germany DA - 2008-10-06 PY - 2008 AN - OPUS4-18102 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Berger, Georg A1 - Acil, Y. A1 - Jepsen, S. A1 - Spitzer, Andrea ED - Ohgushi, H. T1 - Rapidly resorbable and spongiosa-like dicalcium potassium sodium phosphate glass-ceramics for three-dimensional cultivation of human osteoblast-like cells PY - 1999 SN - 981-024099-6 N1 - Serientitel: Bioceramics – Series title: Bioceramics IS - 12 SP - 233 EP - 236 PB - World Scientific CY - Singapore AN - OPUS4-19147 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Symietz, Christian A1 - Lehmann, Erhard A1 - Gildenhaar, Renate A1 - Krüger, Jörg A1 - Berger, Georg T1 - Fixierung resorbierbarer Knochenersatzkeramik auf Ti6Al4V-Implantatmaterial mittels Femtosekundenlaser T2 - 5. Thüringer Grenz- und Oberflächentage und 7. Thüringer Biomaterial-Kolloquium CY - Friedrichroda, Deutschland DA - 2009-09-15 KW - Metallprothese KW - Implantat KW - Knochenersatz KW - Bioaktives Material KW - Lasersintern KW - Femtosekundenlaser PY - 2009 SN - 978-3-00-028446-5 SP - 327 AN - OPUS4-20051 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Dombrowski, Felix A1 - Ploska, Ute A1 - Berger, Georg A1 - Gildenhaar, Renate A1 - Stiller, M. A1 - Knabe, C. ED - Kim, Sukyoung T1 - Rheological investigations on degradable and figuline calcium alkaline phosphate cements T2 - 22nd International symposium on ceramics in medicine (Bioceramics 22) CY - Daegu, Korea DA - 2009-10-26 KW - Rheology KW - Calcium alkaline phosphate cement KW - Gelatine KW - Composite PY - 2009 SP - 863 EP - 866 AN - OPUS4-20287 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Gildenhaar, Renate A1 - Berger, Georg A1 - Lehmann, Erhard A1 - Spitzer, Andrea ED - Kim, Sukyoung T1 - Preparation of calcium alkali phosphate implants by 3D-printing T2 - 22nd International symposium on ceramics in medicine (Bioceramics 22) CY - Daegu, Korea DA - 2009-10-26 KW - Bone substitute material KW - Calcium alkali phosphate KW - Resorbable bioceramics KW - 3D-printing process KW - Bending strength PY - 2009 SP - 71 EP - 74 AN - OPUS4-20288 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Winkler, T. A1 - Hoenig, E. A1 - Gildenhaar, Renate A1 - Berger, Georg A1 - Fritsch, D. A1 - Janssen, R. A1 - Morlock, M.M. A1 - Schilling, A.F. T1 - Volumetric analysis of osteoclastic bioresorption of calcium phosphate ceramics with different solubilities N2 - Commonly, to determine osteoclastic resorption of biomaterials only the resorbed area is measured. The depth of the resorption pit, however, may also be important for the performance of a material. To generate such data we used two calcium phosphate ceramics (Ca10 and Ca2). The solubility of the materials was determined according to DIN EN ISO 10993-14. They were scanned three-dimensionally using infinite focus microscopy and subsequently cultivated for 4 weeks in simulated body fluid without (control) or with human osteoclasts. After this cultivation period osteoclasts number was determined and surface changes were evaluated two- and three-dimensionally. Ca10 and Ca2 showed solubilities of 11.0 ± 0.5 and 23.0 ± 2.2 mg g-1, respectively. Both materials induced a significant increase in osteoclast number. While Ca10 did not show osteoclastic resorption, Ca2 showed an increased pit area and pit volume due to osteoclastic action. This was caused by an increased average pit depth and an increased number of pits, while the average area of single pits did not change significantly. The deduced volumetric osteoclastic resorption rate (vORR) of Ca2 (0.01–0.02 µm3 µm-2 day-1) was lower than the remodelling speed observed in vivo (0.08 µm3 µm-2 day-1), which is in line with the observation that implanted resorbable materials remain in the body longer than originally expected. Determination of volumetric indices of osteoclastic resorption might be valuable in obtaining additional information about cellular resorption of bone substitute materials. This may help facilitate the development of novel materials for bone substitution. KW - Biodegradation KW - Surface analysis KW - Simulated body fluid KW - Osteoclasts KW - Calcium phosphate ceramics PY - 2010 DO - https://doi.org/10.1016/j.actbio.2010.04.015 SN - 1742-7061 VL - 6 IS - 10 SP - 4127 EP - 4135 PB - Elsevier CY - Amsterdam AN - OPUS4-22554 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Quillard, S. A1 - Paris, M. A1 - Deniard, P. A1 - Gildenhaar, Renate A1 - Berger, Georg A1 - Obadia, L. A1 - Bouler, J.-M. T1 - Structural and spectroscopic characterization of a series of potassium- and/or sodium-substituted beta-tricalcium phosphate N2 - In this paper, we report X-ray diffraction investigations as well as Raman and solid-state 31P and 23Na magic angle spinning nuclear magnetic resonance (NMR) characterization of three series of calcium orthophosphates. The general formulae of the studied compounds are Ca10.5-x/2Mx(PO4)7, where M = K or Na and x = 0, 0.25, 0.50, 0.75, 1.0; and Ca10KxNa1-x(PO4)7, where x = 0, 0.25, 0.5, 0.75, 1.0. These calcium orthophosphates are found to be isostructural with β-tricalcium phosphate (β-TCP, Ca3(PO4)2) with the substitution of some calcium sites by potassium and/or sodium cations. The unit cell parameters vary continuously with the level of substitution, a characteristic of these solid solutions. The Raman spectra show the different vibrational bands of the phosphate groups PO4, while the NMR chemical shifts are sensitive to the non-equivalent phosphorus and sodium ions present in these substituted samples. As both Raman and NMR spectroscopies are local probes, they offer tools to distinguish between these different phosphorus and phosphate groups, according to their structural site and local environment, especially the type of cation substituent. A convenient decomposition of the Raman and NMR spectra into Gaussian–Lorentzian components leads us to propose an assignment of the main observed bands of these substituted β-TCPs. KW - Bioceramics KW - Tricalcium phosphate KW - X-ray diffraction KW - Raman spectroscopy KW - Solid-state NMR spectroscopy PY - 2011 DO - https://doi.org/10.1016/j.actbio.2010.12.016 SN - 1742-7061 VL - 7 IS - 4 SP - 1844 EP - 1852 PB - Elsevier CY - Amsterdam AN - OPUS4-23405 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -