TY - JOUR A1 - Bahlmann, Arnold A1 - Falkenhagen, Jana A1 - Weller, Michael G. A1 - Panne, Ulrich A1 - Schneider, Rudolf T1 - Cetirizine as pH-dependent cross-reactant in a carbamazepine-specific immunoassay JF - Analyst N2 - High performance liquid chromatography (HPLC) was hyphenated with a previously reported carbamazepine-specific enzyme-linked immunosorbent assay (ELISA) as a screening approach to water analysis in order to identify possible interferences from transformation products. Treated wastewater was analysed and three substances were recognized by the antibody besides carbamazepine: the metabolites 10,11-dihydro-10,11-epoxycarbamazepine and 2-hydroxycarbamazepine plus the structurally not obviously related antihistamine cetirizine. The molar cross-reactivity against cetirizine was found to be pH-dependent and assessed to be 400% at pH 4.5 and 22% at pH 10.5. Performing the ELISA at pH 10.5 greatly improved the accuracy when carbamazepine was determined in surface and wastewater samples. KW - Immunoassay KW - ELISA KW - Cetirizin KW - Carbamazepin KW - pH-Abhängigkeit KW - Kreuzreaktivität PY - 2011 DO - https://doi.org/10.1039/c0an00928h SN - 0003-2654 SN - 1364-5528 VL - 136 IS - 7 SP - 1357 EP - 1364 PB - Royal Society of Chemistry CY - Cambridge AN - OPUS4-23546 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -