TY - CONF A1 - Sieg, H. A1 - Lehmann, C. A1 - Kästner, Claudia A1 - Krause, B. A1 - Burel, A. A1 - Chevance, S. A1 - Böhmert, L. A1 - Lichtenstein, D. A1 - Tentschert, J. A1 - Bräuning, A. A1 - Laux, A. A1 - Thünemann, Andreas A1 - Loipis, I. E. A1 - Fessard, V. A1 - Luch, A. A1 - Lampen, A. T1 - Effects of Al-, Ti- and Zn-containing nanomaterials on cell lines in vitro T2 - TOXICOLOGY LETTERS N2 - Among the different tested endpoints, Al- and Ticontaining nanomaterials did notshowany toxicity in intestinal cell lines in vitro. Nevertheless, this absence of effect was not due to an absence of exposure, since particle-specific uptake was reported. Metal particle uptake over a long time period might therefore be relevant for risk assessment of aluminum- and titanium-containing food products. T2 - 52nd Congress of the European-Societies-of-Toxicology (EUROTOX) CY - Seville, Spain DA - 04.09.2017 KW - Nanoparticles PY - 2017 DO - https://doi.org/10.1016/j.toxlet.2016.06.1954 SN - 0378-4274 VL - 258 SP - S272 PB - Elsevier Ltd. AN - OPUS4-40939 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Sieg, H. A1 - Kästner, Claudia A1 - Krause, B. A1 - Meyer, T. A1 - Burel, A. A1 - Böhmert, L. A1 - Lichtenstein, D. A1 - Jungnickel, H. A1 - Tentschert, J. A1 - Laux, P. A1 - Braeuning, A. A1 - Estreal-Lopis, I. A1 - Gauffre, F. A1 - Fessard, V. A1 - Meijer, J. A1 - Luch, A. A1 - Thünemann, Andreas A1 - Lampen, A. T1 - Impact of an artificial digestion procedure on aluminum-containing nanomaterials JF - Langmuir N2 - Aluminum has gathered toxicological Attention based on relevant human exposure and its suspected hazardous potential. Nanoparticles from food supplements or Food contact materials may reach the human gastrointestinal tract. Here, we monitored the physicochemical fate of aluminum containing nanoparticles and aluminum ions when passaging an in vitro model of the human gastrointestinal tract. Smallangle X-ray scattering (SAXS), transmission electron microscopy (TEM), ion beam microscopy (IBM), secondary ion beam mass spectrometry (TOF-SIMS), and inductively coupled plasma mass spectrometry (ICP-MS) in the singleparticle mode were employed to characterize two aluminumcontaining nanomaterials with different particle core materials (Al0, γAl2O3) and soluble AlCl3. Particle size and shape remained unchanged in saliva, whereas strong Agglomeration of both aluminum nanoparticle species was observed at low pH in gastric fluid together with an increased ion release. The levels of free aluminum ions decreased in intestinal fluid and the particles deagglomerated, thus liberating primary particles again. Dissolution of nanoparticles was limited and substantial changes of their shape and size were not detected. The amounts of particle-associated phosphorus, chlorine, potassium, and calcium increased in intestinal fluid, as compared to nanoparticles in standard dispersion. Interestingly, nanoparticles were found in the intestinal fluid after addition of ionic aluminum. We provide a comprehensive characterization of the fate of aluminum nanoparticles in simulated gastrointestinal fluids, demonstrating that orally ingested nanoparticles probably reach the intestinal epithelium. The balance between dissolution and de novo complex formation should be considered when evaluating nanotoxicological experiments. KW - Small-angle X-ray scattering KW - SAXS KW - Nanoparticle PY - 2017 DO - https://doi.org/10.1021/acs.langmuir.7b02729 SN - 1520-5827 SN - 0743-7463 VL - 33 IS - 40 SP - 10726 EP - 10735 PB - Americal Chemical Society AN - OPUS4-42438 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lichtenstein, D. A1 - Meyer, Th. A1 - Böhmert, L. A1 - Sieg, H. A1 - Juling, S. A1 - Thünemann, Andreas A1 - Lopies, I. E. A1 - Bräuning, A. A1 - Lampen, A. T1 - Evidence for particle-specific and coating-related uptake of silver nanoparticles T2 - Toxicology Letters N2 - Present data indicate that the quantification of silver nanoparticle internalization revealed a clear particle-specific and coatingrelated uptake. Furthermore, a high amount of silver nanoparticles is taken up in cell models of higher complexity. Thus, an underestimation of particle effects in vitro might be prevented by considering cell models with greater proximity to the in vivo situation. T2 - 52nd Congress of the European-Societies-of-Toxicology (EUROTOX) CY - Seville, Spain DA - 04.09.2016 KW - Small-angle X-ray scattering KW - SAXS KW - Nanoparticle KW - Silver PY - 2017 DO - https://doi.org/10.1016/j.toxlet.2016.06.1938 SN - 0378-4274 VL - 258 SP - S267 EP - S267 PB - Elsevier Ltd. AN - OPUS4-40936 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lichtenstein, D. A1 - Böhmert, L. A1 - Meyer, Th. A1 - Sieg, H. A1 - Thünemann, Andreas A1 - Loipis, I. E. A1 - Bäuning, A. A1 - Lampen, A. T1 - Core or coating material? What dictates the uptake and translocation of nanoparticles in vitro? T2 - Toxicology Letters N2 - A core-dependent effect on nanoparticle translocation was revealed. Both the uptake and transport of nanoparticles in and through cells should be considered when discussing nanoparticle fate and safety. T2 - 52nd Congress of the European-Societies-of-Toxicology (EUROTOX) CY - Seville, Spain DA - 04.09.2016 KW - Small-angle X-ray scattering KW - SAXS KW - Silver KW - Nanoparticle PY - 2017 DO - https://doi.org/10.1016/j.toxlet.2016.06.1940 SN - 0378-4274 SN - 1879-3169 VL - 258 SP - S267 EP - S268 PB - Elsevier Ltd. AN - OPUS4-40935 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -