TY - CONF A1 - Böhmert, L. A1 - Sieg, H. A1 - Braeuning, A. A1 - Lampen, A. A1 - Thünemann, Andreas A1 - Kästner, Claudia T1 - Fluorescence labeling study of silver nanoparticles N2 - During the last years, there has been a rapid rise in the use of nanomaterials in consumer products. Especially silver nanoparticles are frequently used because of their well-known optical and antimicrobial properties. However, the toxicological studies focusing on silver nanoparticles are controversial, either claiming or denying a specific nano-efffect. To contribute to localizing nanoparticles in toxicological studies and to investigate the interaction of particles with cells, a fluorescent marker is often used to monitor their transport and possible degradation. A major problem, in this context is the issue of binding stability of a fluorescent marker which is attached to the particle. In order to overcome this problem we provide an investigation of the binding properties of fluorescence-labeled BSA to small silver nanoparticles. Therefore, we synthesized small silver nanoparticles which are stabilized by poly(acrylic acid). The particles are available as reference candidate material and were thoroughly characterized in an earlier study. The ligand was exchanged by fluorescence marked albumin (BSA-FITC). The adsorption of the ligands was monitored by dynamic light scattering (DLS). To verify that the observed effects on the hydrodynamic radius originate from the successful ligand exchange and not from agglomeration or aggregation we used small angle X-ray scattering (SAXS). The fluorescent particles were characterized by UV/Vis and fluorescence spectroscopy. Afterwards, desorption of the ligand BSA-FITC was monitored by fluorescence spectroscopy and the uptake of particles in different in vitro models was studied. The particles are spherical and show no sign of aggregation after successful ligand exchange. The fluorescence intensity is quenched significantly by the presence of the silver cores as expected, but the remaining fluorescence intensity was high enough to use these particles in biological investigations. Half-life of fluorescence labeling on the particle was 21 d in a highly concentrated solution of non-labeled BSA. Thus, a very high dilution and long incubation times are needed to remove BSA-FITC from the particles. Finally, the fluorescence-labeled silver nanoparticles were used for uptake studies in human liver and intestinal cells, showing a high uptake for HepG2 liver cells and almost no uptake in differentiated intestinal Caco-2 cells. In conclusion, we showed production of fluorescence-marked silver nanoparticles. The fluorescence marker is strongly adsorbed to the silver surface which is crucial for future investigations in biological matrices. This is necessary for a successful investigation of the toxicological potential of silver nanoparticles. T2 - NanoTox 2018 - 9th International Conference on Nanotoxicology CY - Neuss, Germany DA - 18.09.2018 KW - Silver nanoparticles KW - Fluorescence KW - Cell imaging KW - Dynamic light scattering PY - 2018 AN - OPUS4-45639 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Böhmert, L. A1 - Hansen, Ulf A1 - Girod, Matthias A1 - Niemann, B. A1 - Maul, Ronald A1 - Lampen, A. T1 - Der Einfluss eines vitro Verdaus auf die physikochemischen und toxikologischen Eigenschaften von Silbernanopartikeln T2 - 40. Deutscher Lebensmittelchemikertag T2 - 40. Deutscher Lebensmittelchemikertag CY - Halle (Saale), Germany DA - 2011-09-12 PY - 2011 AN - OPUS4-24221 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Böhmert, L. A1 - Niemann, B. A1 - Mantion, Alexandre A1 - Graf, P. A1 - Thünemann, Andreas A1 - Taubert, A. A1 - Lampen, A. T1 - Cytotoxicity of peptide-coated silver nanoparticles in the human intestinal cell line Caco-2 T2 - Post-satellite Meeting Eurotox 2009 T2 - Post-satellite Meeting Eurotox 2009 CY - Dresden, Germany DA - 2010-04-23 PY - 2010 AN - OPUS4-21074 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Böhmert, L. A1 - Hansen, Ulf A1 - Girod, Matthias A1 - Lampen, A. A1 - Knappe, P. T1 - Cytotoxicity of AgPure silver nanoparticles in the human intestinal cell line Caco-2 T2 - Eurotox T2 - Eurotox CY - Paris, France DA - 2011-08-28 PY - 2011 AN - OPUS4-24222 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Voss, L. A1 - Saloga, Patrick E. J. A1 - Stock, V. A1 - Böhmert, L. A1 - Braeuning, A. A1 - Thünemann, Andreas A1 - Lampen, A. A1 - Sieg, H. T1 - Correction to "Environmental impact of ZnO nanoparticles evaluated by in vitro simulated digestion" T2 - ACS Applied Nano Materials N2 - This is a corrigendum to the original article "Environmental impact of ZnO nanoparticles evaluated by in vitro simulated digestion" that was published in the journal "ACS Applied Nano Materials", vol. 3 (2020), no. 1, pp. 724-733. PY - 2020 DO - https://doi.org/10.1021/acsanm.0c01054 VL - 3 IS - 5 SP - 4914 EP - 4914 PB - American Chemical Society CY - Washington, D.C., USA AN - OPUS4-50812 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Paul, M. B. A1 - Fahrenson, C. A1 - Givelet, L. A1 - Herrmann, T. A1 - Loescher, K. A1 - Böhmert, L. A1 - Thünemann, Andreas A1 - Braeuning, A. A1 - Sieg, H. T1 - Beyond microplastics ‑ investigation on health impacts of submicron and nanoplastic particles after oral uptake in vitro JF - Microplastics and Nanoplastics N2 - The continuously increasing use of plastics is supposed to result in a rising exposure of MNPs to humans. Available data on human health risks of microplastics after oral uptake increased immensely in the past years and indicates very likely only low risks after oral consumption. Concerning nanoplastics, uptake, transport and potential adverse effects after oral uptake are less well understood. This study aims to investigate differences between microplastic particles and particles in the submicron- and nanoscaled size derived from food-relevant polymers with a particle size range consistent with higher potential for cellular uptake, fate, and effects when applied to human intestinal and liver cells. This work includes the development of cellular and subcellular detection methods for synthetic polymeric particles in the micro- and nanometer-range, using Scanning Electron Microscopy, Small-Angle X-ray and Dynamic Light Scattering methods, Asymmetric Flow Field Flow Fractionation, octanol-water fractionation, fluorescence microscopy and flow cytometry. Polylactic acid (250 nm and 2 μm (polydisperse)), melamine formaldehyde (366 nm) and polymethylmethacrylate (25 nm) were thoroughly characterized. The submicro- and nanoplastic test particles showed an increased uptake and transport quantity through intestinal cells. Both types of particles resulted in observed differences of uptake behavior, most likely influenced by different lipophilicity, which varied between the polymeric test materials. Toxic effects were detected after 24 h only in overload situations for the particles in the submicrometer range. This study provides further evidence for gastrointestinal uptake of submicro- and nanoplastics and points towards differences regarding bioavailability between microplastics and smaller plastic particles that may result following the ingestion of contaminated food and beverages. Furthermore, the results reinforce the importance for studying nanoplastics of different materials of varying size, surface properties, polymer composition and hydrophobicity. KW - Small-angle X-ray scattering KW - SAXS KW - nanoparticle PY - 2022 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-550741 DO - https://doi.org/10.1186/s43591-022-00036-0 VL - 2 SP - 1 EP - 19 PB - Springer Nature AN - OPUS4-55074 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -