TY - CHAP A1 - Ch’ng, A. C. W. A1 - Konthur, Zoltán A1 - Lim, T. S. T1 - Magnetic Nanoparticle-Based Semi-automated Panning for High-Throughput Antibody Selection N2 - Bio-panning is a common process involved in recombinant antibody selection against defined targets. The biopanning process aims to isolate specific antibodies against an antigen via affinity selection from a phage display library. In general, antigens are immobilized on solid surfaces such as polystyrene plastic, magnetic beads, and nitrocellulose. For high-throughput selection, semi-automated panning selection allows simul-taneous panning against multiple target antigens adapting automated particle processing systems such as the KingFisher Flex. The system setup allows for minimal human intervention for pre-and post-panning steps such as antigen immobilization, phage rescue, and amplification. In addition, the platform is also adaptable to perform polyclonal and monoclonal ELISA for the evaluation process. This chapter will detail the protocols involved from the selection stage until the monoclonal ELISA evaluation with important notes attached at the end of this chapter for optimization and troubleshooting purposes. KW - Phage display KW - Monoclonal antibody KW - Antibody library PY - 2023 U6 - https://doi.org/10.1007/978-1-0716-3381-6_15 VL - 2702 SP - 291 EP - 313 PB - Springer US CY - New York, NY AN - OPUS4-58359 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -