TY - CONF A1 - Ramin, Steffen A1 - Weller, Michael G. T1 - Antibody-based SAW sensor for the detection of explosives N2 - One of the important analytical challenges is the fast and reliable trace detection of explosives in the context of security issues, ammunition disposal, and environmental pollution. Antibodies (Ab) are a promising tool for this purpose and the combination with a surface acoustic wave (SAW) sensor opens the opportunity of highly selective and fast, label-free detection. A robust and sensitive method for the detection of the explosive trinitrotoluene (TNT) was developed. The detection limit was determined to be around 0.5 μg/L. The fast signal response of less than 1 minute shows that this approach is suitable for security and other time-critical applications. In addition, the very low crossreactivity highly reduces the number of false-positives in relation to competing techniques, including sniffer dogs. Due to the multianalyte ability of the SAW system, several explosives might be detected in parallel. Terminal amino groups were functionalized with trinitrophenyl (TNP) groups by reaction with trinitrobenzene sulfonic acid (TNBS). In less than 1 minute, a good signal response was obtained. 50 μL of sample was used. No non-specific interaction with the SAM surface was observed. A complete measuring cycle needed 19 minutes including a surface regeneration step with 50 μL of acetonitrile/water/propionic acid (50:50:1) and 150 μL of SDS solution (0.1 %, pH 1 with 100 mM glycine and 100 mM NaCl). A good long-term stability could be shown for at least 6 hours. Two polyclonal antibodies (R1, R2, affinity purified with Protein A) and a monoclonal antibody (A1.1.1) were tested successfully. A commercially available SAW sensor (sam5 blue, SAW Instruments) was used for liquid handling and detection. Self-assembled monolayers (SAM) of alkanethiol derivatives were prepared on gold surfaces leading to non-fouling and hydrophilic properties, due to attached polyethylene glycol (PEG) residues. A continuous flow of buffer (phosphate-buffered saline, PBS plus Tween 20) of 100 μL/min was applied to the sensor system. TNT antibodies were pre-incubated with the samples containing traces of explosives. Polyclonal and monoclonal antibodies were tested. The limit of detection (LOD) was determined to 0.5 μg/L for all three antibodies (3s from 12 replicates). T2 - ANAKON 2013 CY - Essen, Germany DA - 04.03.2013 KW - Surface acoustic wave sensor KW - SAW KW - Explosives KW - TNT KW - Trinitrotoluene KW - SAM KW - Self-assembled monolayer KW - Gold surface KW - Hexadecanethiol KW - PEG KW - Polyethylene glycol KW - TNBS KW - Hapten KW - Amino-PEG PY - 2013 UR - https://f1000research.com/posters/5-1352 U6 - https://doi.org/https://doi.org/10.7490/f1000research.1112309.1 AN - OPUS4-54677 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Spranger, Holger T1 - THz-ToFD Tomography with Synthetic-Aperture-Aperture N2 - Terahertz-radiation (T-ray) are electromagnetic waves within a frequency range between 0,3 to 6 THz. T-rays are nonionising! They penetrate plastic, paper, ceramic and fabric. It is promising to use T-Rays for CT-applications to reveal uncertainties in dielectric materials. T2 - 8. THz-PhD-meeting CY - Marburg, Germany DA - 06.08.2013 KW - Terahertz Tomography synthetic aperture PY - 2013 AN - OPUS4-36223 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Conradi, Bianca A1 - Schwibbert, Karin A1 - Luebke-Becker, A. A1 - Finger, U. T1 - Monitoring of initial colonization of bacteria on implant materials under standardized conditions T2 - DGBM Jahrestagung 2013 CY - Erlangen DA - 2013-09-26 PY - 2013 AN - OPUS4-35163 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Balcazar Pust, Emilio A1 - Askar, Enis A1 - Schröder, Volkmar T1 - Bestimmung von Explosionskenngrößen von Methan/Sauerstoffgemischen T2 - 11. Fachtagung "Anlagen-, Arbeits- und Umweltsicherheit" CY - Köthen DA - 2013-11-07 PY - 2013 AN - OPUS4-34910 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Maack, Stefan A1 - Krause, Martin A1 - Mayer, K. T1 - Räumliche Kenngrößen des Schallfeldes punktförmiger Ultraschall-Prüfköpfe im Niederfrequenzbereich in Polyamid - Experimentelle Untersuchungen und Simulation T2 - Jahrestagung 2013 "Deutscher Gesellschaft für Zerstörungsfreie Prüfung" CY - Dresden DA - 2013-05-06 PY - 2013 AN - OPUS4-33434 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Winkler, Rajko A1 - Linscheid, M. A1 - Jakubowski, Norbert A1 - Panne, Ulrich T1 - Characterisation of in IgG antibody via ESI-MS T2 - Tag der Chemie CY - Darmstadt, Germany DA - 2013-06-01 PY - 2013 AN - OPUS4-33415 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Winkler, Rajko A1 - Linscheid, M. A1 - Jakubowski, Norbert A1 - Panne, Ulrich T1 - Characterisation of in IgG antibody via ESI-MS T2 - Tag der Chemie CY - Potsdam, Germany DA - 2013-06-27 PY - 2013 AN - OPUS4-33413 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Wolf, Julia A1 - Mielentz, Frank A1 - Milmann, Boris A1 - Wiggenhauser, Herbert T1 - Grundlagenuntersuchungen von Ultraschall-Sensoren in Beton T2 - DGZfP Jahrestagung CY - Dresden DA - 2013-05-06 PY - 2013 AN - OPUS4-32993 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Holzweber, Markus A1 - Hutter, H. A1 - Linert, W. A1 - Dörfler, D. A1 - Lungwitz, R. A1 - Spange, S. A1 - Koel, M. T1 - Mutual Lewis-Acid Base Interaction of Cations and Anions in Ionic LIquids T2 - JCF Berlin 2013 CY - Berlin, Germany DA - 2013-03-06 PY - 2013 AN - OPUS4-32944 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Rohwetter, Philipp A1 - Habel, Wolfgang T1 - Fibre-optic sensors for partial discharge-generated ultrasound in elastomeric high-voltage insulation materials T2 - 5th European Workshop on Optical Fibre Sensors - 5th EWOFS' 2013 CY - Cracow, Poland DA - 2013-05-19 PY - 2013 AN - OPUS4-32611 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -