TY - CONF A1 - Hodoroaba, Vasile-Dan T1 - Available particle-sizing techniques at work for the classification as a nanomaterial - How reliable it really is? N2 - The capability of currently available particle sizing techniques for reliable classification of materials that potentially fall under the EU Definition of a nanomaterial is discussed. A systematic quantitative evaluation of the sizing techniques is presented together with representative case studies of analysis of industrially relevant materials. Recommendations on the most appropriate and efficient use of techniques for different types of material are given. T2 - Frontiers of Nanomaterial Characterization CY - Tokyo, Japan DA - 28.05.2017 KW - Nanomaterial classification KW - Nanoparticles KW - Number-weighted median size KW - Particle size analysis KW - Characterization techniques PY - 2017 AN - OPUS4-40474 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Rades, Steffi T1 - Surface and in-depth analysis of functionalization of TiO2 nanoparticles for self-assembly in multiple layers N2 - Parameters of TiO2 coatings can greatly influence their final performance in largescale applications such as photocatalytic measurements, orthopedic and/or dental prostheses, cell cultures, and dye-sensitized solar cells. From different film deposition procedures, self-assembly of TiO2 NPs in multiple layers was selected for systematic characterization. EDX, AES and ToF-SIMS analysis have been carried out in order to evaluate the functionalization of several types of TiO2 NPs differing in size, shape and surface area. T2 - EMAS 2017 - 15th European Workshop on MODERN DEVELOPMENTS AND APPLICATIONS IN MICROBEAM ANALYSIS and IUMAS-7 Meeting CY - Constance, Germany DA - 07.05.2017 KW - Functionalization KW - Surface analysis KW - Nanoparticles KW - Thin films PY - 2017 AN - OPUS4-40266 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Rades, Steffi T1 - Tracing fluorine at the surface and in the bulk of TiO2 nanoplatelets by means of SEM-EDX, AES and ToF-SIMS N2 - The synthesis of TiO2 nanoplatelets with fluorine-containing reactants is carried out using titanium (IV) butoxide as precursor and concentrated HF as shape controller, the final product requires a working up in order to eliminate or at least to reduce the amount of residual fluorides, which is realized here by well-defined thermal treatment of the samples. Bulk and surface sensitive methods namely scanning electron microscopy with energydispersive X-ray spectroscopy (SEM-EDX), Auger electron spectroscopy (AES) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) have been applied to trace the presence of any fluorides in dependence on different information depths and measurement sensitivities of these methods. T2 - EMAS 2017 - 15th European Workshop on MODERN DEVELOPMENTS AND APPLICATIONS IN MICROBEAM ANALYSIS and IUMAS-7 Meeting CY - Konstanz, Germany DA - 07.05.2017 KW - Nanoparticles KW - Surface analysis KW - ToF-SIMS KW - AES KW - SEM-EDX PY - 2017 AN - OPUS4-40265 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hodoroaba, Vasile-Dan T1 - Improved Deposition of Nanoparticles vy Electrospray Analaysis with SEM/TEM and EDS N2 - Although there are many experimental techniques for measuring particle sizes and size distributions, electron microscopy (EM) is still considered as the gold standard in this field, especially when it comes to particle sizes in the nanorange (1 nm – 100 nm). Furthermore, high-resolution X-ray spectroscopy (EDS) can be applied to individual nanoparticles. To be able to extract accurate information from the EM micrographs and EDS elemental maps that are representative for the material under investigation, one needs to assure the representativity of the particles as sampled on the substrate and their homogeneous spatial distribution, to avoid operator bias when selecting the imaged area. Furthermore, agglomeration should be avoided as far as possible. Several sample preparation techniques exist since a long time, the most common way being suspending the particles in a liquid and depositing them on the grid. However, this procedure includes the drying of larger solvent amounts on the substrate itself, which can affect the spatial distribution of the deposited particles. One possibility to overcome this problem is the use of an electrospray system, where the suspension of particles is sprayed onto the substrate in charged droplets that are so small that they either dry off on the substrate immediately without affecting the position of particles, or even already during their flight time to the substrate. No dedicated commercial instruments are available for the preparation of TEM grids yet, only electrostatic deposition of aerosols on TEM grids has been reported so far. To test the prototype and assess its performance, several materials have been sprayed onto TEM grids and the resulting particle distributions were compared to more traditional sample preparation strategies like the “drop on grid” method. Operation parameters such as the sample flow-rate, capillary – substrate distance, electric field strength and sampling period length have been optimised. It was found that the particles deposited by electrospray generally show a much more homogeneous spatial distribution on the substrate and a substantial increase of the number of single particles, which are much better suited to an automatic image evaluation procedure than the agglomerated particles observed otherwise. The applicability of the technique to a broad range of materials is demonstrated by various examples, but also the influence of the substrate, the choice of the particular TEM grid, on the obtained spatial particle distribution is assessed. T2 - EMAS 2017 - 15th European Workshop on MODERN DEVELOPMENTS AND APPLICATIONS IN MICROBEAM ANALYSIS and IUMAS-7 Meeting CY - Konstanz, Germany DA - 07.05.2017 KW - Electrospray deposition KW - TEM grids KW - Nanoparticles KW - Agglomeration PY - 2017 AN - OPUS4-40257 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Behnke, Thomas T1 - Spectroscopic quantification of surface groups on micro- and nanoparticles N2 - Nanometer- and micrometer-sized particles are of increasing importance for a wide range of applications in the material and life sciences. This includes carriers for, e.g., drugs as well as dye molecules for use as multichromophoric reporters for signal enhancement in optical assays, platforms for DNA sequencing, and the fabrication of nanosensors and targeted probes for bioimaging studies. Application-relevant properties of such particles include their size (and size distribution), shape, colloidal stability, biocompatibility, and ease of subsequent functionalization, e.g., with linkers, sensor molecules, and targeting ligands. The latter requires knowledge of the number of groups effectively accessible for subsequent coupling reactions and hence, selective and sensitive methods of analysis, which can be ideally employed for the characterization of a broad variety of particle systems independent of their optical properties, i.e., scattering or the presence of encoding dyes. For product control, robust, reliable and fast methods performable with inexpensive equipment are prefered. In this respect, we studied a variety of conventional labels for optical readout, utilizing changes in intensity and/or color of absorption and/or emission. In addition, we developed a platform of cleavable and multimodal labels for optical assays which consist of a cleavable linker and an optically active reporter moiety. In contrast to conventional reporters measured directly at the particle surface, which are prone to signal distortions by scattering and encoding dyes, these cleavable labels can be detected colorimetrically or fluorometrically both bound at the particle surface and after quantitative cleavage of the linker in the transparent supernatant. Moreover, they enable straightforward validation by method comparison with elemental analysis, ICP-OES or ICP-MS. Here, we present representative examples of newly synthesized cleavable labels and their application for the quantification of amino, thiol and carboxy surface groups on different nanomaterials and compare these results with measurements using conventional optical labels. T2 - 253rd ACS National Meeting CY - San Francisco, CA, USA DA - 02.04.2017 KW - Nanoparticles KW - Microparticles KW - Surface groups KW - Quantification KW - Cleavable linker PY - 2017 N1 - Geburtsname von Nirmalananthan-Budau, Nithiya: Nirmalananthan, N. - Birth name of Nirmalananthan-Budau, Nithiya: Nirmalananthan, N. AN - OPUS4-40004 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hodoroaba, Vasile-Dan T1 - SETNanoMetro advances: Fabrication and measurement of TiO2 engineered nanoparticles N2 - The press conference on the results of the European Project FP7 constitutes a dissemination event dedicated to the production and characterization of "Shape-engineered TiO2 nanoparticles for metrology of functional properties: setting design rules from material synthesis to nanostructured devices" with the main large-scale applications in the three reference sectors: energy, health and environment. T2 - Press conference on the results of the European Project FP7 CY - Istituto Nazionale di Ricerca Metrologica (INRiM), Torino, Italy DA - 31.03.2017 KW - TiO2 KW - Nanoparticles KW - Measurement KW - Metrology KW - Shape-enegineered nanoparticles PY - 2017 AN - OPUS4-39622 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schneider, Ralf T1 - Investigation and control of protein adsorption for fluorescent nanosilver reference material N2 - Upon interaction of nanomaterials like noble metal nanoparticles (NPs) with biological systems like body fluids such as serum, a protein corona is formed.[1] This reversibly bound layer of proteins controls the transport of the NPs and their subsequent interaction with biological components.[2] The plasmonic properties of nobel metal NPs like Au and Ag can considerably affect the fluorescence properties of fluorophores in their vicinity, i.e., within a near field distance. Depending on the chemical composition, size and shape of these noble metal NPs, the spectral properties of the dye, and the particle-fluorophore distance, the fluorescence is quenched or in some cases enhanced.[3,4,5] This can be monitored by fluorescence intensity and lifetime measurements, with the latter effect being accompanied by an increase in fluorescence intensity and reduction in fluorescence lifetime due to an increase in radaiative rate constant. We utilized these effects to study and manipulate noble metal NP-protein interaction exemplarily for fluorophore-labeled bovine serum albumin (BSA) modified e.g. by succinylation, amination and the introduction of thiol groups, resulting in different binding affinities of the proteins.[6] Our results show that the fluorescent corona allows monitoring of the interaction of our accordingly protein-functionalized particles with biological model systems like solutions containing different amounts of various proteins. This can be eventually used for further in vitro and in vivo studies to assess the uptake, digestion, and excretion of surface functionalized noble metal NPs. T2 - EBS 2017 CY - Potsdam, Germany DA - 20.03.2017 KW - Nanoparticles KW - Reference material KW - Fluorescence probes KW - Plasmonics PY - 2017 AN - OPUS4-39552 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hodoroaba, Vasile-Dan T1 - Analysis of TiO2 nanoparticles – Various activities at BAM N2 - The presentation goes into the characterization of TiO2 engineered nanoparticles within EU/FP7 SETNanoMetro Project and the nanomaterial classification according to the EC definition tested within EU/FP7 NanoDefine Project. Further, ISO/TC 229/JWG 2 activities related on ISO standards in development and inter-laboratory comparisons on measurement of nanoparticle size and shape distribution by SEM and TEM are discussed. T2 - Seminar CY - PTB Braunschweig, Germany DA - 23.03.2017 KW - Nanoparticles KW - Electron microscopy KW - Nanomaterial KW - Standardization KW - Inter-laboratory comparison PY - 2017 AN - OPUS4-39527 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert T1 - Metal detection at cellular levels by use of laser ablation ICP-MS N2 - We are using laser ablation (LA)-ICP-MS to image the local distribution of elements (metals and hetero-elements) directly or (metallo-)proteins by metal-tagged antibodies in cells and tissue indirectly. Different applications will be discussed to demonstrate the state of the art and to visualize the elemental distribution pattern in soft bio-materials (tissue, single cells). In the first application Pt-containing drugs for cancer treatment are investigated and elemental distribution pattern are shown for tissue samples from animal experiments. Different standardization and quantification schemes including isotope dilution analysis will be discussed. In the second application, which is dedicated to toxicological research, the up-take of nano-particles by single cells are discussed and metal containing stains are used to visualize the distribution of nano-particles, proteins and DNA in a single cell simultaneously. This information is correlated with the distribution of the nanoparticles to identify the cell compartments where nano-particles are enriched. Quantification schemes have been developed to transform the measured intensities into number of particles up taken by the cells. In the third and last application LA-ICP-MS is applied to visualize the local distribution of proteins, which are used as bio-markers for prostate cancer. For this purpose, biopsy samples from patients have been simultaneously stained by eight differently metal-tagged antibodies in a multiplex approach. Detection of house-keeping proteins serves as internal standards to overcome differences in protein expression. Additionally ink-jet printing of metal doped inks onto the surface of these tissue samples has been applied for internal standardization and drift corrections. Finally future trends to develop an “elemental microscope” will be discussed. T2 - PITTCON 2017 CY - Chicago, IL, USA DA - 05.03.2017 KW - Element-microscopy KW - LA-ICP-MS KW - Nanoparticles KW - Immuno-assays PY - 2017 AN - OPUS4-39364 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Jakubowski, Norbert T1 - The quantitative elemental microscope: for what is it good for? N2 - Elemental imaging of biological samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) provides spatially resolved information on element distribution in thin sections. By rastering with a laser across the sample, a two-dimensional image of the elemental distribution can be reconstructed that shows the intensities of the respective elements. Using overlapping laser shots the area ablated from single cells or thin sections (thickness 5 to 10 µm) can be reduced significantly so that the pixel size of the intensity measurement is significantly reduced. Having in mind that a laser shot ablates thin biological samples completely, we can make use of a new concept for calibration in the laser ablation method: the concept of total consumption. This calibration strategy allows production of simple matrix matched standards and provides an internal standardisation by ink jet technology, where a metal containing inks is printed on as thin layer on top of a biological sample. Different applications will be presented where our concepts have been applied. In the first example we used nanoparticle suspension of given particle numbers to quantify the uptake of metallic nanoparticles by biological cells. In the second example antibodies have been tagged by metals to measure protein expression in prostata cancer. In this approach application of house keeping proteins are investigated additionally to compensate variations in thickness and density of the biopsy samples. In the third application different nephrotoxic behaviour of Pt containing drugs have been investigated to study the local enrichement in kidney samples of mice treated with these three different compounds. Here the internal standard is required to allow intercomparisons between different individual mouse tissues. At the end of the lecture future trends will be discussed for elemental microscopy. T2 - European Winter Conference on Plasma Spectrochemistry 2017 CY - Sankt Anton am Arlberg, Austria DA - 19.02.2017 KW - LA-ICP-MS KW - Bioimaging KW - Nanoparticles KW - Biomarker PY - 2017 AN - OPUS4-39267 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hübert, Thomas A1 - Kemnitz, Erhard T1 - Wood protection with nanoparticles: MgF2 and CaF2 N2 - The potential of alkaline earth metal Fluoride nanoparticles was tested for protection of wood against fungi and termites. These nanoparticles are ideal for wood protection. T2 - School of Analytical Sciences Poster Session CY - Berlin, Germany DA - 06.12.2016 KW - Wood preservation KW - Fluorides KW - Nanoparticles PY - 2016 AN - OPUS4-39141 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - Standardization of Fluorescence Measurements in the UV/vis/NIR/IR - Needs for and requirements on calibration tools N2 - Photoluminescence techniques are amongst the most widely used tools in the life sciences, with new and exciting applications in medical diagnostics and molecular imaging continuously emerging. Advantages include their comparative ease of use, unique sensitivity, non-invasive character, and potential for multiplexing, remote sensing, and miniaturization. General drawbacks are, however, signals, that contain unwanted wavelength- and polarization contributions from instrument-dependent effects, which are also time-dependent due to aging of instrument-components, and difficulties to measure absolute fluorescence intensities. Moreover, scattering systems require special measurement geometries and the interest in new optical reporters with emission > 1000 nm strategies for reliable measurements in the second diagnostic for the comparison of material performance and the rational design of new fluorophores with improved properties. Here, we present strategies to versatile method-adaptable liquid and solid fluorescence standards for different fluorescence parameters including traceable instrument calibration procedures and the design of integrating sphere setups for the absolute measurement of emission spectra and quantum yields in the wavelength region of 350 to 1600 nm. Examples are multi-emitter glasses, spectral fluorescence standards, and quantum yield standards for the UV/vis/NIR T2 - Spie Photonics west 2017 CY - San Francisco, USA DA - 28.01.2017 KW - Fluorescence standard KW - Instrument calibration KW - Integrating sphere spectroscopy KW - Absolute fluorescence quantum yield KW - Fluorescent glasses KW - Nanoparticles PY - 2017 AN - OPUS4-39074 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - RPRT A1 - Babick, F. A1 - Mielke, Johannes A1 - Hodoroaba, Vasile-Dan A1 - Weigel, St. A1 - Wohlleben, W. T1 - Critical review manuscript with real-world performance data for counting, ensemble and separating methods including in-build mathematical conversion to number distributions submitted for publication N2 - The content of the paper is the assessment of the performance of (conventional) measurement techniques (MTs)with respect to the classification of disperse materials according to the EC recommendation for a definition of nanomaterial. This performance essentially refers to the accurate assessment of the number weighted median of (the constituent) particles. All data and conclusions are based on the analytical study conducted as real-world performance testing. It comprised different types of MTs (imaging, counting, fractionating, spectroscopic and integral) as well as different types of materials. Beside reference materials with well-defined size distribution the study also included several commercial powders (variation of particle composition, morphology, coating, size range and polydispersity). In order to ensure comparability of measurement results, the participants were guided to use uniform protocols in sample preparation, conducting measurements, data analysis and in reporting results. Corresponding documents have been made public, in order to support the reviewing process of the paper, respectively to ensure the reproducibility of data by other users under the same conditions. The scientific paper relies on a comprehensive set of revised measurement data reported in uniform templates, completely describes the experimental procedures and discusses the MTs’ performance for selected materials in detail. Even more, the study is summarised and evaluated, which leads to recommendations for the use of MTs within a tiered approach of NM characterisation. In addition, the paper critically examines the factors that may affect the outcome of such a comparison among different MTs. KW - Nanomaterial KW - Measurement techniques KW - EC definition of nanomaterial KW - Nanoparticles PY - 2016 UR - https://nbn-resolving.org/urn:nbn:de:kobv:b43-389646 UR - http://www.nanodefine.eu/index.php/downloads/nanodefine-technical-reports UR - http://www.nanodefine.eu/publications/reports/NanoDefine_TechnicalReport_D3.3.pdf SP - D3.3, 1 EP - 72 PB - The NanoDefine Consortium CY - Wageningen, The Netherlands AN - OPUS4-38964 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Schmidt, R. T1 - SEM or TEM for the characterization of nanoparticles? N2 - Scanning Electron Microscopy (SEM) or Transmission Electron Microscopy (TEM) ? This is a question, nowadays discussed in EM labs of research and industry involved in the characterization and metrology of nanoparticles. The Scanning principle is adapted to TEM, the Transmission mode is adapted to Ultra High Resolution SEM. Can modern SEM replace TEM, reach atomic resolution even without Cs corrector or nm lateral resolution for X-ray Spectroscopy (EDX)? Due to the development of a New Cold Field Emission (NCFE) electron source Hitachi SEMs SU9000 and SU8200 can deliver routinely sub-nm image resolution and EDX mappings at very high count rates and a lateral EDX resolution of a few nm. A TiO2 sample provided by BAM was analysed at 30 kV using low kV STEM – simultaneously with the Through-the-lens (TTL) SE detector, the Bright Field transmission and Dark Field transmission signals. By this method a pixel-precise information of the particle’s surface using SE, its chemical nature using DF-STEM and its crystalline structure using Bright Field signal is given in one 40sec scan. The advantage of this observation mode compared to Ultra Low Voltage imaging is outlined. T2 - 7. NRW Nano-Konferenz CY - Münster, Germany DA - 07.12.2016 KW - SEM KW - TEM KW - Nanoparticles PY - 2016 AN - OPUS4-38936 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hodoroaba, Vasile-Dan T1 - Proposal of an inter-laboratory comparison on shape of bipyramidal TiO2 nanoparticles by TEM N2 - A proposal of an inter-laboratory study (ILC) on determination of size and shape distribution of TiO2 nanoparticles (NPs) by transmission electron microscopy is presented. The anatase NPs synthesized in a controllable fashion within the EU/FP7 project SETNanoMetro can be considered as shape-defined (bipyramidal) and are offered to complete the list of case studies already in progress within ISO/TC229/JWG2. The main points of the measurement procedure are presented as well as a proposed procedure to evaluate the size and shape according to the standard operation procedure already developed within SETNanoMetro is discussed. Potential ILC participants and a plan with next step to be carried out are proposed. T2 - Joint Meeting IEC TC 113 'Nanotechnology for electrotechnical products and systems' and ISO/TC229/JWG2 'Nanotechnologies'/'Measurement and characterization' CY - Lowell, MA, USA DA - 09.05.2016 KW - Nanoparticles KW - TiO2 KW - TEM KW - Shape KW - Inter-laboratory comparison KW - ISO PY - 2016 AN - OPUS4-38900 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Hodoroaba, Vasile-Dan T1 - Inter-laboratory comparison on shape of bipyramidal TiO2 nanoparticles by TEM N2 - The TiO2 material selected for the present inter-laboratory comparison (ILC) was the bipyramidal anatase UT001 batch synthesized hydrothermally by University of Turin in the frame of the EU/FP7 Project SETNanoMetro and carefully prepared on TEM grids by BAM to be distributed to the ILC participants. A clear measurement protocol including data analysis and reporting (including an xls reporting template) has been also prepared by BAM. 14 of 18 participants have measured and reported results which have been already checked by BAM as being conform to the agreed protocol. After receiving the last results (of 4 participants) the ILC data evaluation will be carried out by BAM in line with agreed ISO/TC229 procedures as for the other case sudies. T2 - Annual Meeting of ISO/TC 229 Nanotechnologies CY - Singapore, Republic of Singapore DA - 07.11.2016 KW - TiO2 KW - Nanoparticles KW - Inter-laboratory comparison KW - Shape KW - TEM KW - Particle size distribution PY - 2016 AN - OPUS4-38868 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Rades, Steffi T1 - Analysis of functionalization of TiO2 nanoparticles and various substrates by means of SEM-EDX, AES and ToF-SIMS N2 - The physico-chemical characteristics of TiO2 coatings can greatly influence their final performance. In SETNanoMetro, different deposition procedures are being set for applying films of TiO2 NPs with defined and homogenous thickness on supports of interest for the applications studied in the project. The selected substrates are the following: (i) Silica glasses for photocatalytic measurements, (ii) Ti-alloys for orthopedic and/or dental prostheses, and for cell cultures, and (iii) Conductive glasses (e.g. Fluorine doped Tin Oxide, FTO) for dye-sensitized solar cells. From the different film deposition procedures studied within the project Self-assembly of TiO2 NPs in multiple layers was selected. For this, surface modification of the substrate and of TiO2 nanoparticles (NPs) with e.g. silane coupling agents is a prerequisite. First attempts to prepare the self-assembled coating on the functionalized glass substrates seem to indicate that the functionalized NPs adhere to the substrates, even if the final coatings were not homogenous and presented agglomerates. ToF-SIMS results support this outcome. In order to use the layer-by-layer deposition technique for the formation of TiO2 films by controlled self-assembly of the TiO2 NPs, the proper complementary moieties for the functionalization of the NPs were chosen. A first set of NPs has been produced by reaction with (3-aminopropyl)phosphonic acid (APPA) in order to functionalize the surface with free amino-groups. Then, the complementary NP set can be obtained from an aliquot of the first one, through the conversion of free surface amino-groups to aldehydes by reaction with glutaraldehyde. A proper approach for the functionalization of two types of TiO2, commercial P25 (Evonik) and SETNanoMetro-sample labelled UT001, with APPA was developed. A second set of NPs consisting of three types of TiO2 NPs, P25 and SETNanoMetro NPs (TiO2 NPs with high specific surface area > 150 m2/g and TiO2 NPs with low size < 20 nm) was functionalized with (3-aminopropyl)triethoxysilane (APTS). As for the previous set of NPs, the complementary NP set was obtained through the conversion of free surface amino-groups to aldehydes by reaction with glutaraldehyde. EDX, AES and ToF-SIMS spectra were collected and analyzed to demonstrate the presence of the surface functionalization of the different types of TiO2 NPs. T2 - Nanoscience meets Metrology - Synthesis, Characterization, Testing and Applications of Validated Nanoparticles - International Summer School CY - Turin, Italy DA - 04.09.2016 KW - Time-of-flight secondary-ion mass spectrometry KW - X-ray spectroscopy KW - Auger electron spectroscopy KW - Electron microscopy KW - Nanoparticles KW - Thin films PY - 2016 UR - http://www.setnanometro.eu/events/ AN - OPUS4-38064 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Natte, Kishore A1 - Friedrich, Jörg Florian A1 - Wohlrab, Sebastian A1 - Lutzki, J. A1 - von Klitzing, R. A1 - Österle, Werner A1 - Orts-Gil, Guillermo T1 - Impact of polymer shell on the formation and time evolution of nanoparticle-protein corona N2 - The study of protein corona formation on nanoparticles (NPs) represents an actual main issue in colloidal, biomedical and toxicological sciences. However, little is known about the influence of polymer shells on the formation and time evolution of protein corona onto functionalized NPs. Therefore, silicapoly(ethylene glycol) core–shell nanohybrids (SNPs@PEG) with different polymer molecular weights (MW) were synthesized and exhaustively characterized. Bovine serum albumin (BSA) at different concentrations (0.1–6 wt%) was used as model protein to study protein corona formation and time evolution. For pristine SNPs and SNPs@PEG (MW = 350 g/mol), zeta potential at different incubation times show a dynamical evolution of the nanoparticle–protein corona. Oppositely, for SNPs@PEG with MW ≥2000 g/mol a significant suppression of corona formation and time evolution was observed. Furthermore, AFM investigations suggest a different orientation (side-chain or perpendicular) and Penetration depth of BSA toward PEGylated surfaces depending on the polymer length which may explain differences in protein corona evolution. KW - Nanoparticles KW - Silica KW - PEGylation KW - Protein corona KW - BSA KW - Biointerface PY - 2013 DO - https://doi.org/10.1016/j.colsurfb.2012.11.019 SN - 0927-7765 VL - 104 SP - 213 EP - 220 PB - Elsevier AN - OPUS4-38547 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Mielke, Johannes A1 - Dohányosová, P. A1 - Lopez, S. A1 - Hodoroaba, Vasile-Dan T1 - Electrospray deposition of nanoparticles on TEM grids N2 - The authors have tested the prototype of an electrospray deposition system developed by the company RAMEM under its trademark IONER. To test the prototype and assess its performance, several materials have been sprayed onto TEM grids and the resulting particle distributions were compared to more traditional sample preparation strategies like the “drop on grid” method. Operation parameters such as the sample flow-rate, capillary – substrate distance, electric field strength and sampling period length have been optimized. It was found that the particles deposited by electrospray generally show a much more homogeneous spatial distribution on the substrate and a substantial increase of the number of single particles, which are much better suited to an automatic image evaluation procedure than the agglomerated particles observed otherwise. The applicability of the technique to a broad range of materials is demonstrated by various examples, but also the influence of the substrate, the choice of the particular TEM grid, on the obtained spatial particle distribution is assessed. KW - Electrspray deposition KW - Electron microscopy KW - Nanoparticles KW - Sample preparation PY - 2016 UR - https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/electrospray-deposition-of-nanoparticles-on-tem-grids/459E634B7F74D474A19E15E69DA82E5D DO - https://doi.org/10.1017/S1431927616010072 SN - 1431-9276 SN - 1435-8115 VL - 22 IS - Suppl 3 SP - 1846 EP - 1847 PB - Cambridge AN - OPUS4-38436 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Iannarelli, L. T1 - Shape engineered TiO2 nanoparticles in Caenorhabditis elegans: a Raman imaging based approach to assist tissue-specific toxicological studies N2 - In vivo detailed spatial distribution of TiO2 NPs was here investigated for the first time using a 2D chemical imaging analysis based on Confocal Raman Spectroscopy. The invertebrate nematode C. elegans was employed as prototypical model of living organism. Rod, bipyramidal and quasi-spherical engineered TiO2 NPs with different primary particle sizes and agglomeration states were prepared, characterized and then administered to nematodes. Exploiting the typical fingerprint of TiO2 in the Raman spectrum, we monitored the biodistribution of NPs inside the worm using a non-invasive, label-free method. The high spatial resolution chemical imaging and the specificity of the Raman technique in the localization of TiO2 NPs helped in the design of behavioral C. elegans studies aimed at elucidating the relationship among the size, shape, and agglomeration state of NPs and their ability to induce specific toxic effects. T2 - 16th European Conference on Applications of Surface and Interface Analysis ECASIA'15 CY - Fortaleza, Brazil DA - 14.08.2016 KW - TiO2 KW - Nanoparticles KW - Raman imaging KW - Toxicological studies PY - 2016 AN - OPUS4-38240 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -