TY - GEN A1 - Lisec, Jan T1 - IsoCor N2 - Despite numerous advantages offered by hyphenation of chromatography and electrokinetic separation methods with multicollector (MC) ICP-MS for isotope analysis, the main limitation of such systems is the decrease in precision and increase in uncertainty due to generation of short transient signals. To minimize this limitation, most authors compare several isotope ratio calculation methods and establish a multi-step data processing routine based on the precision and accuracy of the methods. However, to the best of our knowledge, there is no universal data processing tool available that incorporates all important steps of the treatment of the transient signals. Thus, we introduce a data processing application (App) IsoCor that facilitates automatic calculation of isotope ratios from transient signals and eases selection of the most suitable method. The IsoCor App performs baseline subtraction, peak detection, mass bias correction, isotope ratio calculation and delta calculation. The feasibility and reliability of the App was proven by reproducing the results from isotope analysis of three elements (neodymium, mercury and sulfur) measured on-line via hyphenated systems. The IsoCor App provides trackability of the results to ensure quality control of the analysis. KW - Shiny-App KW - ICP-MS KW - Software PY - 2022 UR - https://github.com/cran/IsoCor/ PB - GitHub CY - San Francisco, CA, USA AN - OPUS4-56307 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Ecke, Alexander A1 - Westphalen, Tanja A1 - Retzmann, Anika A1 - Schneider, Rudolf T1 - The Fate of the Antibiotic Amoxicillin in the Aquatic Environment N2 - Contamination of the environment with antibiotics is of great concern as it promotes the evolution of antimicrobial resistances. In case of amoxicillin (AMX) in the aquatic environment, further risk arises from hydrolysis products (HPs) which can cause allergy. To assess these risks, a comprehensive investigation and understanding of the degradation of AMX is necessary. We investigated the hydrolysis rate of AMX in different types of water as well as the influence of temperature and irradiation. The content of the heavy metal ions copper and zinc was found to be crucial for the hydrolysis rate of AMX and stability of HPs. Eventually, a new degradation pathway for AMX could be elaborated and confirmed by tandem mass spectrometry (LC-MS/MS). T2 - Berliner Chemie in Praxis Symposium CY - Berlin, Germany DA - 07.10.2022 KW - Hydrolysis KW - Amoxicillin KW - LC-MS/MS KW - ICP-MS PY - 2022 AN - OPUS4-56026 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike A1 - Saatz, Jessica A1 - Ascher, Lena A1 - Boyraz, B. A1 - Hahndorf, J. A1 - Schnorr, J. A1 - Schellenberger, E. A1 - Tauber, R. T1 - Unraveling the interaction of MRI contrast agents with tissue using LA ICP MS N2 - Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is increasingly used to study the distribution of metal-containing drugs, imaging probes and nanomaterials in connection with disease related changes and therapy progress. Additionally, biomolecules can be detected indirectly by using metal-tagged antibodies. The extracellular matrix (ECM) is, besides the cells, an important component of all body tissues. The macromolecular network of the ECM consists of structural proteins (e.g., collagen, elastin) and proteoglycans composed of highly negatively charged carbohydrates, the glycosaminoglycans (GAGs), which are covalently linked to a protein core. Many diseases, including inflammatory processes and tumors, are associated with characteristic ECM changes at an early stage. Recent studies have shown that contrast agents for magnetic resonance imaging (MRI), which are based on gadolinium containing chelate complexes or iron oxide nanoparticles, can bind themselves to ECM components. To elucidate the role of GAGs like keratan sulfate (KS) and its modification state in disease, highly specific tools are necessary. As a complement to conventional immunohistochemistry LA-ICP-MS was applied to investigate the distribution of KS in tissue thin sections using a well characterized anti-KS antibody labelled with metal ions. Furthermore, LA-ICP-MS was used for the detection of MRI contrast agents and the identification of their target cells and molecules in tissue samples from animal models, e.g. for cardiovascular diseases. The results show the possibilities of LA-ICP-MS for the elucidation of pathological tissue changes. T2 - European Workshop on Laser Ablation (EWLA 2022) CY - Berne, Switzerland DA - 12.07.2022 KW - Laser ablation KW - Imaging KW - ICP-MS KW - Antibody PY - 2022 AN - OPUS4-55315 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CHAP A1 - Theiner, S. A1 - Corte Rodriguez, M. A1 - Traub, Heike ED - Golloch, A. T1 - Novel applications of lanthanoids as analytical or diagnostic tools in the life sciences by ICP-MS based techniques N2 - Inductively coupled plasma-mass spectrometry (ICP-MS) is a well-established analytical method offering high sensitivity and multi-element analysis. ICP-MS has found acceptance in various application areas ranging from material analysis to applications in the life sciences. Within the last 15 years new strategies for the sensitive detection and accurate quantification of biomolecules in complex biomedical samples have been developed. Recent instrumental improvements have contributed to this progress. As most of the biomolecules do not contain endogenous metals etectable with ICP-MS, bioconjugation with artificial metal-containing tags based on metal-loaded chelate complexes or nanoparticles is increasingly applied to determine biomolecules indirectly. Especially, the combination of immunohistochemical workflows using lanthanoid-tagged antibodies and ICP-MS detection provides new insights in the complexity and interdependency of cellular processes. Single-cell ICP-MS, also termed as mass cytometry, allows high-dimensional analysis of biomarkers in cell populations at single-cell resolution. For that purpose, lanthanoid isotope labelled antibodies are used to detect their corresponding target molecules. The visualisation of the elemental distribution is possible with laser ablation ICP-MS (LA-ICPMS) at high spatial resolution. Especially, the combination of LA with ICP time-of-flight mass spectrometry, also referred to as imaging mass cytometry (IMC), opens new possibilities for multiparametric tissue imaging at the single-cell level and even below. The lanthanoid localisation and concentration can be linked to their conjugated antibody target providing valuable information about surface markers, intracellular signalling molecules to measure biological function, and the network state of an individual cell in a tissue. This book chapter focuses on new applications, where the multi-element capabilities of ICP-MS are used for the detection of lanthanoids applied as artificial elemental stains or tags for biomolecules and in particular antibodies. KW - ICP-MS KW - Laser ablation KW - Cell KW - Antibody KW - Immunohistochemistry KW - Lanthanoid KW - Mass cytometry KW - Imaging PY - 2022 SN - 978-3-11069-645-5 SN - 978-3-11069-636-3 DO - https://doi.org/10.1515/9783110696455-013 SP - 399 EP - 444 PB - De Gruyter CY - Berlin, Boston ET - 2. rev. and exten. edition AN - OPUS4-55118 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Brauckmann, C. A1 - Pramann, A. A1 - Rienitz, O. A1 - Schulze, A. A1 - Phukphatthanachai, P. A1 - Vogl, Jochen T1 - Combining Isotope Dilution and Standard Addition - Elemental Analysis in Complex Samples N2 - A new method combining isotope dilution mass spectrometry (IDMS) and standard addition has been developed to determine the mass fractions w of different elements in complex matrices: (a) silicon in aqueous tetramethylammonium hydroxide (TMAH), (b) sulfur in biodiesel fuel, and (c) iron bound to transferrin in human serum. All measurements were carried out using inductively coupled plasma mass spectrometry (ICP–MS). The method requires the gravimetric preparation of several blends (bi)—each consisting of roughly the same masses (mx,i) of the sample solution (x) and my,i of a spike solution (y) plus different masses (mz,i) of a reference solution (z). Only these masses and the isotope ratios (Rb,i) in the blends and reference and spike solutions have to be measured. The derivation of the underlying equations based on linear regression is presented and compared to a related concept reported by Pagliano and Meija. The uncertainties achievable, e.g., in the case of the Si blank in extremely pure TMAH of urel (w(Si)) = 90% (linear regression method, this work) and urel (w(Si)) = 150% (the method reported by Pagliano and Meija) seem to suggest better applicability of the new method in practical use due to the higher robustness of regression analysis. T2 - CITAC Best Paper Award Ceremony CY - Online meeting DA - 21.06.2022 KW - Isotope dilution mass spectrometry KW - Standard addition KW - ICP-MS KW - Blank characterization KW - Silicon KW - Sulfur KW - Transferrin KW - Tetramethylammonium hydroxide KW - Biodiesel fuel KW - Human serum PY - 2022 AN - OPUS4-55032 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - GEN A1 - Völzke, Jule Lexa T1 - Herstellung und Charakterisierung Antigen-beschichteter Nanopartikel als Analoga von Virus-like Particles (VLP) N2 - In dieser Arbeit wurden drei verschiedene Nanomaterialien auf ihre Bindungsfähigkeit zu Proteinen untersucht. Zu Beginn standen dabei die Herstellung stabiler Dispersionen der einzelnen Nanopartikel und die Stabilität der gebildeten Konjugate im Vordergrund. Der Nachweis einer erfolgreichen Konjugatbildung, sprich der Beschichtung von Nanopartikel mit Proteinen, wurde sowohl qualitativ mittels DLS-Messungen als auch über quantitative Protein-Bestimmungen erbracht. Für die Quantifizierung konnten verschiedene Methoden eingesetzt werden. Neben der klassischen Vorgehensweise, welche indirekt über die Quantifizierung von ungebundenem Protein im Überstand erfolgt, konnten ihm Rahmen dieser Arbeit verschiedene direkte Bestimmungsmethoden entwickelt werden. So wurden mittels kolorimetrischer Tests, wie dem BCA-Assay und dem Bradford-Assay, Nanodiamantdispersionen mit Hilfe einer Korrekturwellenlänge vermessen und quantifiziert. Ebenso zum Einsatz kam die Methode der Aminosäureanalytik, welche aufgrund ihrer guten Rückführbarkeit auf Aminosäurestandards Ergebnisse mit hoher Richtigkeit generieren kann und ebenso die Detektion kleiner Proteinmengen möglich macht. Nach den erfolgten quantitativen Betrachtungen wurden die Protein-beschichteten Nanopartikel auf ihre Anwendbarkeit als Analoga von Virus-like Particles (VLP) bei einer Immunisierung zur Gewinnung von polyklonalen Antikörpern gegen humanes Ceruloplasmin in Kaninchen überprüft. Es konnte mittels ELISA gezeigt werden, dass die Konjugate erfolgreich für die Herstellung von Antikörpern eingesetzt werden können und im zeitlichen Verlauf einer Immunisierung eine Steigerung des Antikörper-Titers zu erreichen ist. KW - DLS KW - Dynamische Lichtstreuung KW - Aluminiumoxid KW - Nanodiamant KW - Gold-Nanopartikel KW - BSA KW - Albumin KW - Protein G KW - Ceruloplasmin KW - Immunpräzipitation KW - Ultraschall KW - BCA KW - Bradford-Assay KW - AAAA KW - Aromatische Aminosäureanalytik KW - ICP-MS KW - NaCl-Methode PY - 2018 SP - 1 EP - 107 PB - Humboldt-Universität zu Berlin CY - Berlin AN - OPUS4-54625 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Seeger, Stefan T1 - Analysis of air pollutants in ambient and indoor aerosolsby TXRF - application examples N2 - Quantitative chemical analysis of airborne particulate matter (PM) is vital for the understanding of health effects in indoor and outdoor environments, as well as for enforcing air quality regulations. Typically, airborne particles are sampled over long time periods on filters, followed by lab-based analysis, e.g., with inductively coupled plasma mass spectrometry (ICP-MS). Within the EURAMET EMPIR AEROMET project, cascade impactor aerosol sampling was combined for the first time with on-site total reflection X-ray fluorescence (TXRF) spectroscopy to develop a tool for quantifying particle element compositions within short time intervals and even on-site. This makes variations of aerosol chemistry observable with time resolution of only a few hours and with good size resolution in the PM10 range. A proof of principles of this methodological approach and the comparison to standard methods within the scope of a field campaign will be presented. Secondly, aerosol sampling and TXRF analysis seems suitable for the quantification of elements in indoor aerosols as well and may provide an important enhancement of existing methods for the analysis of organic species in aerosols (such as sampling and TD-GC/MS). As an example, the TXRF analysis of particles emitted from laser printers under controlled conditions in an environmental test chamber will be presented. T2 - TXRF Journal ClubB CY - Online meeeting DA - 24.02.2022 KW - Aerosol KW - TXRF KW - Cascade impactor KW - ICP-MS KW - Particles KW - Air quality monitoring KW - Element mass concentration PY - 2022 AN - OPUS4-54418 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Wetzel, Annica A1 - Özcan Sandikcioglu, Özlem A1 - Witt, Julia A1 - Rhode, Michael T1 - Corrosion Properties and Protective Oxide Film Characteristics of CrMnFeCoNi High Entropy Alloy and CrCoNi Medium Entropy Alloy N2 - High and medium entropy alloys gained increasing academic and industrial interest as novel materials for engineering applications. This project is aiming to clarify and compare the general and local corrosion properties of high entropy alloy CrMnFeCoNi and medium entropy alloy CrCoNi in different aqueous environments. The focus lies on the local corrosion processes that result either from microstructural imperfections (inclusions, defects at grain boundaries etc.) in the base material or processing related changes in the microstructure and/or local composition. The corrosion behavior of the alloys was monitored via potentiodynamic polarization experiments and the local corrosion characteristics were further investigated by means of scanning electrochemical microscopy (SECM). Their passivation behavior was analyzed in three different electrolyte systems (NaCl, H2SO4 and NaClO4; c = 0.1M). The characterization of the surface morphology and composition of the passive film was performed by means of atomic force microscopy (AFM), scanning electron microscopy coupled with energy dispersive X-Ray spectroscopy (SEM/EDX) and X-Ray photoelectron spectroscopy (XPS), respectively. Considering long term corrosion effects, electrochemical work was supported with immersion tests and the analysis of corrosion products by SEM/EDX and XPS depth-profiling. Our results indicate that the medium entropy alloy CrCoNi has a significantly higher corrosion resistance due to the higher concentration of Chromium in comparison to the high entropy alloy CrMnFeCoNi. The presentation will summarize our results on the mechanistical aspects of the observed high corrosion resistance. T2 - EUROCORR 2021 CY - Online meeting DA - 20.09.2021 KW - High Entropy Alloys KW - Aqueous Corrosion KW - Medium Entropy Alloys KW - Atomic Forc Microscopy KW - Scanning Kelvin Probe Force Microscopy KW - Potentiodynamic Polarization KW - Electrochemical Impedance Spectroscopy KW - ICP-MS PY - 2021 AN - OPUS4-53791 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Wetzel, Annica A1 - Özcan Sandikcioglu, Özlem A1 - Witt, Julia A1 - Rhode, Michael T1 - Corrosion Properties and Protective Oxide Film Characteristics of CrMnFeCoNi High Entropy Alloy and CrCoNi Medium Entropy Alloy N2 - High and medium entropy alloys gained increasing academic and industrial interest as novel materials for engineering applications. This project is aiming to clarify and compare the general and local corrosion properties of high entropy alloy CrMnFeCoNi and medium entropy alloy CrCoNi in different aqueous environments. The focus lies on the local corrosion processes that result either from microstructural imperfections (inclusions, defects at grain boundaries etc.) in the base material or processing related changes in the microstructure and/or local composition. The corrosion behavior of the alloys was monitored via potentiodynamic polarization experiments and the local corrosion characteristics were further investigated by means of scanning electrochemical microscopy (SECM). Their passivation behavior was analyzed in three different electrolyte systems (NaCl, H2SO4 and NaClO4; c = 0.1M). The characterization of the surface morphology and composition of the passive film was performed by means of atomic force microscopy (AFM), scanning electron microscopy coupled with energy dispersive X-Ray spectroscopy (SEM/EDX) and X-Ray photoelectron spectroscopy (XPS), respectively. Considering long term corrosion effects, electrochemical work was supported with immersion tests and the analysis of corrosion products by SEM/EDX and XPS depth-profiling. Our results indicate that the medium entropy alloy CrCoNi has a significantly higher corrosion resistance due to the higher concentration of Chromium in comparison to the high entropy alloy CrMnFeCoNi. The presentation will summarize our results on the mechanistical aspects of the observed high corrosion resistance. T2 - EuroMat 2021 CY - Online meeting DA - 12.09.21 KW - High Entropy Alloys KW - Aqueous Corrosion KW - Medium Entropy Alloys KW - Atomic Forc Microscopy KW - Scanning Kelvin Probe Force Microscopy KW - Potentiodynamic Polarization KW - Electrochemical Impedance Spectroscopy KW - ICP-MS PY - 2021 AN - OPUS4-53790 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Wetzel, Annica A1 - Özcan Sandikcioglu, Özlem A1 - Witt, Julia A1 - Rhode, Michael T1 - Corrosion Properties and Protective Oxide Film Characteristics of CrMnFeCoNi High Entropy Alloy and CrCoNi Medium Entropy Alloy N2 - High and medium entropy alloys gained increasing academic and industrial interest as novel materials for engineering applications. This project is aiming to clarify and compare the general and local corrosion properties of high entropy alloy CrMnFeCoNi and medium entropy alloy CrCoNi in different aqueous environments. The focus lies on the local corrosion processes that result either from microstructural imperfections (inclusions, defects at grain boundaries etc.) in the base material or processing related changes in the microstructure and/or local composition. The corrosion behavior of the alloys was monitored via potentiodynamic polarization experiments and the local corrosion characteristics were further investigated by means of scanning electrochemical microscopy (SECM). Their passivation behavior was analyzed in two different electrolyte systems (NaCl and H2SO4 c = 0.1M). The characterization of the surface morphology and composition of the passive film was performed by means of atomic force microscopy (AFM), scanning electron microscopy coupled with energy dispersive X-Ray spectroscopy (SEM/EDX) and X-Ray photoelectron spectroscopy (XPS), respectively. To analyze the semiconducting properties of the passive film Mott-Schottky analysis was conducted. Considering long term corrosion effects, electrochemical work was supported with immersion tests and the analysis of corrosion products by SEM/EDX, ICP-MS and XPS depth-profiling. Our results indicate that the medium entropy alloy CrCoNi has a significantly higher corrosion resistance due to the higher concentration of chromium in comparison to the high entropy alloy CrMnFeCoNi. The presentation will summarize our results on the mechanistical aspects of the observed high corrosion resistance. T2 - ISE Annual 72nd meeting CY - Online meeting DA - 29.08.2021 KW - High Entropy Alloys KW - Aqueous Corrosion KW - Medium Entropy Alloys KW - Atomic Forc Microscopy KW - Scanning Kelvin Probe Force Microscopy KW - Potentiodynamic Polarization KW - Electrochemical Impedance Spectroscopy KW - ICP-MS PY - 2021 AN - OPUS4-53789 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Seeger, Stefan A1 - Osan, J. A1 - Czömpöly, O. A1 - Gross, A. A1 - Stoßnach, H. A1 - Stabile, L. A1 - Ochsenkuehn-Petropoulou, M. A1 - Tsakanika, L. A. A1 - Lymperopoulou, T. A1 - Goddart, S. A1 - Fiebig, M. A1 - Gaie-Levrel, F. A1 - Rissler, J. A1 - Kayser, Y. A1 - Beckhoff, B. T1 - Element mass concentrations in ambient aerosols, a comparison of results from filter sampling & ICP-MS ans cascade impactor sampling & mobile total reflection X-RAY fluorescence spectroscopy N2 - Quantitative chemical analysis of airborne particulate matter (PM) is vital for the understanding of health effects in indoor and outdoor environments and required by EU air quality regulations. Typically, airborne particles are sampled on filters, followed by lab-based analysis, e.g., with inductively coupled plasma mass spectrometry (ICP-MS). Within the EURAMET EMPIR AEROMET project, cascade impactor aerosol sampling was combined with on-site total reflection X-ray fluorescence (TXRF) spectroscopy. The study aimed at a proof of principles for this new mobile and on-size tool for the quantification of aerosol element compositions and element mass concentrations within short time intervals of less than 12 h. In a field campaign the method’s technical feasibility could be demonstrated. The TXRF results were traced back to a stationary, reference-free XRS setup in the laboratory of the German national metrology institute PTB at the BESSY II electron storage ring in Berlin, Germany. Simultaneous PM10-filter sampling, followed by standardized lab-based analysis, allowed for a comparison of the field campaign data of both methods. As Fig. 1 shows, the correspondence between PM10 filter sampling and ICP-MS, and on the other hand, cascade impactor sampling and TXRF is quite encouraging. However, for some of the analysed elements, e.g. V and Pb, the observed deviations are higher than expected and this highlights the fact, that spectral deconvolution strategies for TXRF on cascade impactor samples still need some improvement. This work was supported by the EMPIR programme, co-financed by the Participating States and from the European Union’s Horizon 2020 research and innovation programme, through grant agreements 16ENV07 AEROMET and 19ENV08 AEROMET II T2 - 12th International Conference on Instrumental Methods of Analysis (IMA-2021) CY - Athens, Greece DA - 20.09.2021 KW - Aerosol KW - TXRF KW - Reference method KW - Cascade impactor KW - Ambient aerosols KW - Air quality monitoring KW - Element mass concentration KW - Size resolved chemical composition KW - Time resolved chemical composition KW - ICP-MS PY - 2021 AN - OPUS4-53597 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CHAP A1 - Traub, Heike ED - Milacic, R. ED - Scancar, J. ED - Goenaga-Infante, H. ED - Vidmar, J. T1 - Imaging of metal-based nanoparticles in tissue and cell samples by laser ablation inductively coupled plasma mass spectrometry N2 - Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is nowadays a versatile and powerful analytical method for direct solid sample analysis. The applicability has been demonstrated for a wide variety of samples covering hard and soft materials. In an imaging mode the technique provides quantitative information on the elemental distribution within a sample. LA-ICP-MS imaging is of particular interest in biomedical research as the distribution of an element gives valuable insight on uptake and distribution of essential and toxic trace elements, administered contrast agents as well es nanoparticles. LA-ICP-MS is therefore a powerful complement to other imaging techniques. Recent instrumental improvements, especially in sample chamber design, have contributed to better sensitivity and spatial resolution enabling subcellular imaging. The book chapter provides a comprehensive overview about spatially resolved localisation and quantification of various nanoparticles in cells and tissue thin sections by LA-ICP-MS. Furthermore, different sample preparation strategies and internal standardisation and calibration approaches for bioimaging by LA-ICP-MS are summarized and discussed. Metal-containing nanomaterials are used in numerous fields ranging from industrial applications to nanomedicine. Several studies have demonstrated that the physicochemical properties of nanoparticles have an impact on their pharmacokinetics, transfer and clearance. The high sensitivity and multielement capability of LA-ICP-MS enables the elucidation of interactions between tissue components and nanomaterials used as imaging probes or drug carriers. Potential toxic effects are investigated as well. Thus, LA imaging significantly supports the clinical translation of safe and efficient nanoparticles for diagnostic and therapeutic purposes. KW - Laser ablation KW - ICP-MS KW - Imaging KW - Nanoparticle KW - Nanomaterial KW - Tissue KW - Cell PY - 2021 SN - 978-0-323-85305-7 SN - 0166-526X VL - 93 SP - 173 EP - 240 PB - Elsevier CY - Amsterdam ET - 1 AN - OPUS4-52775 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Blanchard, V. A1 - Traub, Heike A1 - Biskup, K. A1 - Wieczorek, M. A1 - Saatz, Jessica A1 - Pagel, K. T1 - Central project for biochemical analysis of proteoglycans and glycosaminoglycans and for element-specific microscopy N2 - Nearly all disease processes are associated with variations of components of the extracellular matrix (ECM) that are typically observed during the development of inflammation. This concerns for example proteoglycans and their associated glycosaminoglycans (GAG), which have been shown to bind to cationic metal imaging probes due to their strong complexing activity. The complexing activity largely depends on the degree of GAG sulfation and/or carboxylation as well as on the GAG isomericity. In this central project, we investigate GAG structures from inflammatory disorders (namely cardiovascular diseases, inflammatory intestinal diseases and neuroinflammation) provided by researchers of the Collaborative Research Center at the molecular disaccharidic level using chromatographic and mass spectrometric methods. In parallel, the spatial localization and quantification of metal-based imaging probes are evaluated by LA-ICP-MS imaging. T2 - 1st International Symposium In vivo Visualization of Extracellular Matrix Pathology CY - Online Meeting DA - 27.05.2021 KW - Laser ablation KW - ICP-MS KW - MALDI PY - 2021 AN - OPUS4-52716 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Jakubowski, N. A1 - Borrmann, S. A1 - Recknagel, Sebastian A1 - Roik, Janina A1 - Rickert, F. T1 - Comparison of peristaltic pumps used for sample introduction in Inductively Coupled Plasma-Atomic Emission Spectroscopy (ICP-AES) N2 - In this investigation, two conventional peristaltic pumps are compared with a new pump based on the “easy click” principle using a simultaneous ICP-AES instrument with standard operating conditions. It is found that the figures of merit achieved are quite comparable for all three pumps. Relative standard deviations (RSDs) range between 0.2% and 1.8%, and limits of detection as low as 0.1 μg/L have been achieved , demonstrating that the easy click principle of the new pump does not compromise the analytical figures of merit. KW - ICP-AES KW - Peristaltic pumps KW - ICP-MS PY - 2020 IS - 35 / S4 SP - 6 AN - OPUS4-52020 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lemke, Nora A1 - El-Khatib, Ahmed A1 - Theuring, F. A1 - Jakubowski, Norbert A1 - Vogl, Jochen T1 - Sulfur isotope dilution ICP MS for traceable protein quantification N2 - Inductively coupled plasma mass spectrometry (ICP-MS) is a powerful method for the matrix-independent quantitative analysis of target elements. Developed for the use in inorganic trace analysis, ICP-MS is nowadays a valuable tool for bioanalytical questions. Especially the use of ICP-MS for quantitative proteomics by measuring heteroatoms has gained recognition in the last decade, considering that established quantification methods like organic mass spectrometry depend on labelling of the target protein or the existence of matched protein and peptide standards. The need for reliable quantification of proteins is continuously growing, but only a limited number of well-characterized and quantified protein standards are available so far. Accurately quantified, traceable protein standards are necessary to ensure comparability of measurements between laboratories, not only in basic research but also in a clinical context. One example of this is the Alzheimer’s disease biomarker tau protein. However, existing tau standards lack comparability, emphasizing the need for a well-quantified protein standard. Therefore, we developed a method for the quantification of pure proteins via sulfur isotope dilution ICP-MS (IDMS). As sulfur is present in two amino acids, cysteine and methionine, it exists in nearly all proteins and can be used for the quantification of proteins of known stoichiometry. We employed simple offline strategies for the separation of non-protein bound sulfur species. Quantification of these contaminations by IDMS allows for correction of the protein content and enables reliable protein quantification. We report the protein mass fractions of a standard reference material and commercially available proteins determined by sulfur IDMS, including the expanded uncertainties. The developed method can be applied for the reliable and traceable quantification of pure proteins for use as in-house standards. Here, we successfully used this method for the quantification of the tau protein. T2 - 53rd annual conference of the DGMS including 27th ICP-MS User's Meeting CY - Münster, Germany DA - 01.03.2020 KW - ICP-MS KW - Isotope dilution KW - Protein KW - Quantification KW - Tau protein PY - 2020 AN - OPUS4-50510 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Meermann, Björn A1 - Wichmann, K. A1 - Lauer, F. A1 - Tremel, W. A1 - Vanhaecke, F. A1 - von der Au, M. A1 - Schwinn, M. A1 - Borovinskaya, O. A1 - Büchel, C. A1 - Kuhlmeier, K. T1 - Neue ICP-MS basierte Methoden zur Analyse von Nano- und Mikropartikeln in der Umwelt N2 - In den vergangen Jahren hat das Umweltbewusstsein in der Bevölkerung stark zugenommen und somit auch das Interesse an der Vermeidung von anthropogenen (Schad-)Stoffen in der Umwelt. Eine (neue) Substanzklasse, deren Umweltauswirkungen noch nicht vollständig untersucht sind und die in den vergangenen Jahren immer mehr an Bedeutung gewonnen hat, sind (metallbasierte) Nanomaterialien. Im Gegensatz zu bspw. Elementspezies weisen Nanomaterialien eine Vielzahl von Eigenschaften auf und lassen sich nicht über nur ein Merkmal beschreiben - dies stellt eine große analytische Herausforderung dar. Hier haben sich vor allem die Feld-Fluss-Fraktionierung (AF4) und die single-particle-ICP-MS als leistungsstarke analytische Methoden herausgestellt. In (aquatischen) Umweltmatrizes (z.B. Oberflächengewässern) liegen neben artifiziellen auch natürliche Partikel vor, was eine weitere große Herausforderung für den Nachweis von Nanomaterialien darstellt. Neben dem Nachweis von anthropogenen Stoffen in der Umwelt ist zudem deren ökotoxikologische Bewertung wichtig. In der aquatischen Ökotoxikologie werden hierzu Testorganismen mit den jeweiligen Substanzen über die Wasserphase exponiert. Effektkonzentrationen (EC50) werden dabei auf Basis der Konzentrationen in der Wasserphase abgeleitet - tatsächlich bioakkumulierte Mengen werden hierbei jedoch meist nicht ermittelt; eine weitere große Herausforderung besteht zudem in der Bewertung von Mischungstoxizitäten. Gängige Testorganismen sind u.a. Kieselalgen (Diatomeen). Diatomeen stehen am Anfang der Nahrungskette - toxikologisch relevante Metalle/Nanomaterialien können sich hierüber im Nahrungsnetz der Oberflächengewässer anreichern und ggf. nachhaltig auswirken. Im ersten Teil des Vortrages werden zunächst neue elementanalytische Methoden zum Nachweis von metallbasierten Nanopartikeln in Umweltmatrizes auf Basis der AF4/ICP-SFMS sowie stabilen Isotopenlabeln am Beispiel von Eisennanopartikeln vorgestellt. Im zweiten Teil wird eine neue elementanalytische Methode als komplementäre Technik zur ökotoxikologischen Bewertung von (Schad-)Stoffen vorgestellt. Die neue Methode basiert auf der on-line Kopplung von HPLC mit der single-cell-ICP-(ToF)-MS (sc-ICP-(ToF)-MS) [1, 3-5]. Hierüber konnten wir erfolgreich die automatisierte Multielementanalytik einzelner Diatomeen realisieren und zur Analyse von mit Metallen inkubierten Diatomeen (cyclotella meneghiniana) einsetzen. Wir konnten zeigen, dass die sc-ICP-ToF-MS zukünftig eine leistungsstarke, komplementäre Technik in der aquatischen Ökotoxikologie zum z.B. Test von Metallen und Nanomaterialien darstellt. T2 - GDCh Wissenschaftsforum Chemie CY - Aachen, Germany DA - 17.09.2019 KW - ICP-MS KW - Nanomaterialien KW - Single particle-ICP-ToF-MS KW - Single cell-ICP-ToF-MS PY - 2019 AN - OPUS4-49743 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Völzke, Jule L. A1 - El-Khatib, Ahmed H. A1 - Vogl, Jochen A1 - Weller, Michael G. T1 - Immunoprecipitation with Nanodiamonds for the Enrichment of Ceruloplasmin from Human Serum N2 - In this study, we developed a new immunoprecipitation method based on protein-coated nanodiamonds. Performing SDS-PAGE and using the SDS-PAGE buffer as an eluent showed that CER could be successfully enriched from human serum. Based on the copper determination with ICP-MS, the amount of bound CER on the nanodiamonds can be calculated. We could show the fulfilled mass balance of bound CER and CER in the supernatant after incubation with a known amount of CER. For isotope ratio analysis this method can be applied to compare ratios of the total copper content in human serum to copper ratios from CER enrichment. T2 - ReMiND 2019 CY - Braunschweig, Germany DA - 26.06.2019 KW - Metalloprotein KW - Copper KW - Affinity enrichment KW - Immunoaffinity KW - Protein g KW - Blood KW - Serum KW - Plasma KW - SDS-PAGE KW - ICP-MS PY - 2019 UR - https://www.ptb.de/empir/remind-conference.html AN - OPUS4-49728 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Traub, Heike A1 - Drescher, D. A1 - Büchner, T. A1 - Müller, L. A1 - Wanka, Antje A1 - Hösl, S. A1 - Ascher, Lena A1 - Cruz-Alonso, M. A1 - Pisonero, J. A1 - Kneipp, J. A1 - Jakubowski, Norbert T1 - Imaging of biological samples by LA-ICP-MS N2 - In recent years, elemental imaging of biological samples like tissue thin sections using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is gaining more and more importance. Improvements concerning spatial resolution as well as signal-to-background ratio due to low-dispersion sample chambers make LA-ICP-MS also interesting for single cell analysis. To evaluate the interaction of nanoparticles (NPs) with cells LA-ICP-MS was applied for the imaging of individual cells. Our findings show, that NP aggregates can be localized within cellular compartments. The uptake efficiency depends strongly on the physico-chemical properties of the nanostructures (size, chemical composition, surface modification), as well as on the incubation conditions (concentration, time). Moreover, LA-ICP-MS is increasingly becoming an important complementary technique in bioanalysis by using element-tagging strategies to determine biomolecules indirectly. Based on the specific binding between antibodies and their corresponding antigens, proteins and peptides can be detected in tissue or cells using tagged antibodies. As artificial tags metal chelates loaded with lanthanides, polymer-based elemental tags or metal-containing nanoparticles can be used. Thereby LA-ICP-MS is a sensitive detection tool for multiplexed immuno-histochemistry of tissue and cell samples. Our results demonstrate the potential of LA-ICP-MS to investigate the distribution of naturally occurring elements, administered agents as well as biomolecules by using metal-tagged antibodies. T2 - Workshop on tandem LIBS/LA-ICP-MS 2019 CY - Berlin, Germany DA - 18.11.2019 KW - Laser ablation KW - ICP-MS KW - Nanoparticles KW - Imaging PY - 2019 AN - OPUS4-49704 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Lemke, Nora A1 - El-Khatib, Ahmed A1 - Theuring, F. A1 - Jakubowski, Norbert A1 - Vogl, Jochen T1 - Development of a method for protein quantification via isotope dilution ICP-MS for application on an Alzheimer’s biomarker N2 - Inductively coupled plasma mass spectrometry (ICP-MS) is a powerful method for the matrix-independent quantitative analysis of target elements. Developed for the use in inorganic trace analysis, ICP-MS is nowadays emerging as a valuable tool for bioanalytical questions. Especially the use of ICP-MS for quantitative proteomics by measuring heteroatoms has gained popularity in the last decade, considering that established quantification methods like organic mass spectrometry depend on the existence of matched protein and peptide standards or labelling of the target protein. The need for reliable quantification of proteins is constantly growing, but only a limited number of well characterized and quantified protein standards are available so far. Not only in basic research, but also in a clinical context, accurately quantified, traceable protein standards are needed to ensure comparability of measurements between laboratories. One disease with a major impact on our ageing society is Alzheimer’s disease (AD), which is still challenging to diagnose. As this is also due to a lack in comparability and accuracy of existing biomarker assays, the community would greatly benefit from well quantified protein biomarker standards. In this work, we applied isotope dilution analysis (IDA) using ICP-MS to quantify proteins of known stoichiometry via their sulfur content. Sulfur is present in two amino acids, cysteine and methionine, and hence exists in nearly all proteins. Simple strategies were employed for the detection of low molecular sulfur species to correct for sulfur contaminants and allow for reliable quantification of various proteins. We report the protein mass fractions with expanded uncertainties of a standard reference material and commercially available proteins determined by sulfur IDA. The herein developed method can be applied for the reliable and traceable quantification of pure proteins and will be used for the quantification of an AD biomarker. Our target is the tau protein, as brain load and distribution of tau is highly correlated with the clinical progression of AD. T2 - ReMiND 2019 Biomolecules in Neurodegenerative Diseases CY - Braunschweig, Germany DA - 26.06.2019 KW - ICP-MS KW - Isotope dilution KW - Proteins KW - Quantification PY - 2019 AN - OPUS4-48464 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Arakawa, Akihiro A1 - Jakubowski, Norbert A1 - Flemig, Sabine A1 - Koellensperger, G. A1 - Theiner, S. A1 - Schweikert, A. A1 - Iwahata, D. A1 - Traub, Heike A1 - Hirata, T. T1 - Localization of cells and exposed nanoparticles in three-dimensional in vitro tissue analog measured by means of an elemental microscopy based on LA-ICP-TOF-MS N2 - Metallic nanoparticles (NPs) are currently applied in a variety of consumer products and are also attractive for medical applications. With their widespread use, the potential for human exposure to NPs — either intended or unintended — is increasing. Therefore, many studies have evaluated the toxicity and transport mechanism of NPs. In comparison with two-dimensional cultured cells, multicellular spheroids (MCS) look promising to be used as a three-dimensional cellular model, having unique advantages in nanoparticle studies due to the fact that interactions with excreted extracellular matrix can be investigated. Fibroblast cells are one of the most important cell systems to express a microenvironment by excreting an abundant extracellular matrix. For bioimaging laser ablation inductively coupled-plasma mass spectrometry (LA-ICP-MS) is used in this investigation to study the interaction of metallic NPs with MCS for multi-element detection offering a wide dynamic range. As a mass spectrometer we have applied a time-of-flight (TOF) instrument for (quasi-) simultaneous detection of all isotopes of elements of interest. The aim of this study is to investigate the localization of silver (Ag) NPs exposed to fibroblast MCSs by means of LA-ICP-TOF-MS. In addition, for demonstrating elemental microscopy we chose phosphorous (31P) and iron (56Fe) to visualize regions of enriched extracellular matrix and single cells, respectively. In this presentation, we show that exposed Ag NPs are highly accumulated at the same position of single cells in an outer rim of fibroblast MCSs. T2 - 7th International Symposium on Metallomics CY - Warsaw, Poland DA - 30.06.2019 KW - Nanoparticle KW - Laser ablation KW - ICP-MS KW - Multicellular spheroid PY - 2019 AN - OPUS4-48425 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -