TY - CONF A1 - Linck, Lena A1 - Resch-Genger, Ute T1 - Optimized Fluorescence Detection for RCA-Assays on Microarrays T2 - Kolloquium T2 - Kolloquium CY - Potsdam, Germany DA - 2010-08-25 PY - 2010 AN - OPUS4-22081 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Pauli, Jutta A1 - Würth, Christian A1 - Güttler, Arne A1 - Resch-Genger, Ute T1 - Reliable Determination of the Signal-Relevant Spectroscopic Key Characteristics of Luminescent Reporters and Optical Probes for Imaging in the vis/NIR/SWIR N2 - Introduction. Comparing different emitter classes and rationally designing the next generation of molecular and nanoscale probes for bioimaging applications require accurate and quantitative methods for the measurement of the key parameter photoluminescence quantum yield f.1 f equals the number of emitted per number of absorbed photons. This is particularly relevant for increasingly used fluorescence imaging in the short wave-infrared region (SWIR) ≥ 900 nm providing deeper penetration depths, a better image resolution, and an improved signal-to-noise or tumor-to-background ratio.2, 3 However, spectroscopic measurements in the SWIR are more challenging and require specific calibrations and standards. T2 - EMIM 2021 CY - Göttingen, Germany DA - 24.08.2021 KW - Fluorescence KW - Optical probe KW - Dye KW - Photophysics KW - Quantum yield KW - Mechanism KW - NIR KW - SWIR KW - Imaging KW - Reference material KW - Reliability KW - Nano KW - Particle KW - Method KW - Quality assurance PY - 2021 AN - OPUS4-53233 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Petrov, Eugene A1 - Würth, Christian A1 - Richter, Maria A1 - Resch-Genger, Ute T1 - Fluorescence Standards from BAM - Dye Solutions and Polymer systems N2 - For fluorescence microscopy, there is an increasing need for suitable calibration tools and reference materials for microscope calibration, the determination of performance parameters, and the regular control and validation of instrument performance. This is addressed in the BmWk-financed project FluMikal (WIPANO program) by two research groups from academia and two companies, that is coordinated by BAM. Here we present different approaches to liquid and solid fluorescence standards for the determination of the wavelength-dependent spectral sensitivity of fluorescence microscopes and ideas concerning the choice of suitable fluorescence lifetime standard for increasingly utilized fluorescence lifetime imaging (FLIM). T2 - FluMiKal Projectmeeting CY - Munich, Germany DA - 27.11.2023 KW - Reference material KW - Reference product KW - Fluorescence microscopy KW - Quality assurance KW - Instrument performance validation KW - Lifetime standard PY - 2023 AN - OPUS4-59120 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Pfeifer, Dietmar A1 - Monte, Christian A1 - Hoffmann, Angelika A1 - Spieles, Monika T1 - EN ISO/IEC 17025 Imposed Requirements on Fluorometry and Need for Reference Materials: KIT Spectral Fluorescence Standards T2 - Fluorescence Workshop, BERM-9 T2 - Fluorescence Workshop, BERM-9 CY - Berlin, Germany DA - 2003-06-06 PY - 2003 AN - OPUS4-3761 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Glanz, Mario T1 - Lanthanoidocene als Katalysatoren für neue Polymermaterialien T2 - DFG-Antragskolloquium SPP 1166 T2 - DFG-Antragskolloquium SPP 1166 CY - Bonn, Germany DA - 2003-11-27 PY - 2003 AN - OPUS4-3762 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Pfeifer, Dietmar A1 - Monte, Christian A1 - Hoffmann, Katrin A1 - Hoffmann, Angelika A1 - Spieles, Monika T1 - Traceable Standards for Fluorescence-Based Analysis T2 - Workshop: Fluorescence Standards for the Life Sciences T2 - Workshop: Fluorescence Standards for the Life Sciences CY - London, England DA - 2004-03-10 PY - 2004 AN - OPUS4-4011 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Nitschke, R. T1 - Standardization, quantification and reproducibility in fluorescence widefield and confocal microscopy T2 - Konferenz Focus on Microscopy FOM T2 - Konferenz Focus on Microscopy FOM CY - Jena, Germany DA - 2005-03-20 PY - 2005 AN - OPUS4-7433 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - Nitschke, R. A1 - Engel, A. T1 - Determination of the Spectral Characteristics of Confocal Spectral Imaging Systems and Comparability of Data T2 - Konferenz Focus on Microscopy FOM T2 - Konferenz Focus on Microscopy FOM CY - Jena, Germany DA - 2005-03-20 PY - 2005 AN - OPUS4-7434 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - DeRose, P. T1 - Exploratory Study Spectral Correction T2 - PITTCON 2005 T2 - PITTCON 2005 CY - Orlando, FL, USA DA - 2005-02-27 PY - 2005 AN - OPUS4-7431 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Bremser, Wolfram A1 - Pfeifer, Dietmar A1 - Monte, Christian A1 - Hoffmann, Angelika T1 - Exploratory study "spectral correction of emission spectra": State-of-the-art in fluorescence measurements T2 - Fachbereichskolloquium an der Physikalisch-Technischen Bundesanstalt T2 - Fachbereichskolloquium an der Physikalisch-Technischen Bundesanstalt CY - Berlin, Germany DA - 2006-01-13 PY - 2006 AN - OPUS4-12066 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Angelika A1 - Pfeifer, Dietmar A1 - Hoffmann, Katrin A1 - Monte, Christian T1 - Standards for fluorescence analysis: From steady state fluorometry to fluorescence microscopy T2 - Fraunhofer Institut für Angewandte Polymerforschung T2 - Fraunhofer Institut für Angewandte Polymerforschung CY - Potsdam, Germany DA - 2006-02-08 PY - 2006 AN - OPUS4-12068 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Pfeifer, Dietmar A1 - Hoffmann, Katrin A1 - Monte, Christian T1 - Standardization of fluorescence techniques: Where do we stand and what do we need? T2 - 9th International Conference on Methods and Applications of Fluorescence T2 - 9th International Conference on Methods and Applications of Fluorescence CY - Lisbon, Portugal DA - 2005-09-04 PY - 2005 AN - OPUS4-10828 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Bremser, Wolfram A1 - Pfeifer, Dietmar A1 - Monte, Christian A1 - Hoffmann, Angelika T1 - State-of-the-Art in Fluorescence Measurements T2 - IUPAC Task Force Group Fluorescence T2 - IUPAC Task Force Group Fluorescence CY - Lisbon, Portugal DA - 2005-09-04 PY - 2005 AN - OPUS4-10829 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Pfeifer, Dietmar A1 - Monte, Christian A1 - Hoffmann, Katrin A1 - Hoffmann, Angelika A1 - Spieles, Monika T1 - Rückführbare spektrale Fluoreszenzstandards zur Ermittlung der spektralen Korrekturfunktionen von Fluoreszenzmesssystemen T2 - Einladung zur Fachgruppe "Standardisierung und Quantifizierung in der Mikroskopie" T2 - Einladung zur Fachgruppe "Standardisierung und Quantifizierung in der Mikroskopie" CY - Wetzlar, Germany DA - 2004-09-09 PY - 2004 AN - OPUS4-4073 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Pfeifer, Dietmar A1 - Monte, Christian A1 - Hoffmann, Katrin A1 - Hoffmann, Angelika A1 - Spieles, Monika T1 - Spektrale Fluoreszenzstandards zur rückführbaren Charakterisierung von Fluoreszenzmesssystemen T2 - Einladung zur 3. Arbeitssitzung der Arbeitsgruppe "Fluoreszenz" T2 - Einladung zur 3. Arbeitssitzung der Arbeitsgruppe "Fluoreszenz" CY - Dortmund, Germany DA - 2004-09-20 PY - 2004 AN - OPUS4-4074 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - Nitschke, R. T1 - Simple Tools for the Determination of the Spectral Characteristics of Confocal Spectral Imaging Systems, Instrument Performance and Instrument Long-Term Stability T2 - Workshop T2 - Workshop CY - Darmstadt, Germany DA - 2005-06-03 PY - 2005 AN - OPUS4-7572 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - Pfeifer, Dietmar T1 - Fluorescence Standards: Classification and Relevance for Fluorescence Measurements T2 - Seminar T2 - Seminar CY - Mainz, Germany DA - 2005-06-02 PY - 2005 AN - OPUS4-7573 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - Analytische Applikationen der Fluorometrie: Entwicklung von Fluoreszenzstandards und Fluoreszenzmarkern T2 - Kolloquiumsvortrag am Institut für Radiochemie, Forschungszentrum Rossendorf T2 - Kolloquiumsvortrag am Institut für Radiochemie, Forschungszentrum Rossendorf CY - Dresden, Germany DA - 2003-10-15 PY - 2003 AN - OPUS4-7231 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - Nitschke, R. A1 - Pfeifer, Dietmar A1 - Engel, A. A1 - Hoffmann, Angelika T1 - Characterization of Confocal Spectral Imaging Systems with Liquid and Solid Fluorescence Standards T2 - Arbeitskreis Mikroskopie T2 - Arbeitskreis Mikroskopie CY - Göttingen, Germany DA - 2004-12-15 PY - 2004 AN - OPUS4-5356 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hollandt, J. A1 - Taubert, D. A1 - Pfeifer, Dietmar A1 - Monte, Christian A1 - Hoffmann, Angelika A1 - Spieles, Monika T1 - Traceability in fluorometry: Physical and chemical transfer standards T2 - Arbeitskreis Mikroskopie T2 - Arbeitskreis Mikroskopie CY - Göttingen, Germany DA - 2004-12-15 PY - 2004 AN - OPUS4-5357 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Würth, Christian A1 - Hatami, Soheil A1 - Hennig, Andreas A1 - Hoffmann, Angelika T1 - Surface-functionalized flourescent particles as tools for bioanalytics - Characterization strategies and different applications T2 - 7th Senftenberger Innovationsforum Multiarameteranalytik T2 - 7th Senftenberger Innovationsforum Multiarameteranalytik CY - Senftenberg, Germany DA - 2013-04-18 PY - 2013 AN - OPUS4-28463 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Behnke, Thomas A1 - Würth, Christian A1 - Pauli, Jutta A1 - Hennig, Andreas A1 - Hoffmann, Angelika A1 - Hoffmann, Katrin T1 - Optical Spectroscopy and Functional Dye Systems for Applications in the Life and Material Sciences T2 - TU Dresden AK Eychmüller T2 - TU Dresden AK Eychmüller CY - Dresden, Germany DA - 2013-01-30 PY - 2013 AN - OPUS4-28464 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Behnke, Thomas A1 - Würth, Christian A1 - Hennig, Andreas A1 - Hatami, Soheil A1 - Grabolle, Markus A1 - Hoffmann, Angelika A1 - Spieles, Monika T1 - Design, Spectroscopic and Analytical Characterization of Functional Dye Systems for Applications in the Life Sciences and for Biosensing T2 - FDH Krefeld T2 - FDH Krefeld CY - Krefeld, Germany DA - 2013-05-23 PY - 2013 AN - OPUS4-28465 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Würth, Christian A1 - Behnke, Thomas A1 - Hennig, Andreas A1 - Hatami, Soheil A1 - Hoffmann, Katrin A1 - Spieles, Monika A1 - Hoffmann, Angelika T1 - Characterization of fluorescent particles, IR dyes, and fluorescence standards with integrating sphere spectroscopy T2 - 7th Senftenberger Innovationsforum Multiparameteranalytik T2 - 7th Senftenberger Innovationsforum Multiparameteranalytik CY - Senftenberg, Germany DA - 2013-04-18 PY - 2013 AN - OPUS4-28467 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hennig, Andreas A1 - Würth, Christian A1 - Hatami, Soheil A1 - Hoffmann, Angelika A1 - Jäger, Christian A1 - Borcherding, H. A1 - Schedler, U. A1 - Huber, A. T1 - Characterization of the application-relevant properties of fluorescent particles using simple spectroscopic procedures T2 - Characterization and Bioanalytic Applications of Luminescent (Nano)Probes Chebana – UpCore Joint Seminar T2 - Characterization and Bioanalytic Applications of Luminescent (Nano)Probes Chebana – UpCore Joint Seminar CY - Regensburg, Germany DA - 2012-08-30 PY - 2012 AN - OPUS4-26374 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Behnke, Thomas A1 - Würth, Christian A1 - Hennig, Andreas A1 - Pauli, Jutta A1 - Hoffmann, Katrin A1 - Hatami, Soheil A1 - Grabolle, Markus A1 - Spieles, Monika A1 - Hoffmann, Angelika T1 - Functional Dye Systems for Applications in the Life Sciences and for Biosensing T2 - Heiligenstädter Kolloquium T2 - Heiligenstädter Kolloquium CY - Heilbad Heiligenstadt, Germany DA - 2012-09-24 PY - 2012 AN - OPUS4-26375 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Behnke, Thomas A1 - Würth, Christian A1 - Hennig, Andreas A1 - Hatami, Soheil A1 - Hoffmann, Angelika A1 - Hoffmann, Katrin A1 - Spieles, Monika A1 - Pauli, Jutta T1 - Absolute fluorometry in the life and material sciences: Characteriza¬tion of fluorescent particles, IR dyes, and fluorescence standards T2 - ANAKON 2013 T2 - ANAKON 2013 CY - Essen, Germany DA - 2013-03-04 PY - 2013 AN - OPUS4-27854 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Würth, Christian A1 - Hoffmann, Katrin A1 - Grabolle, Markus A1 - Pauli, Jutta T1 - Fast and reliable measurement of photoluminescence quantum yields for the development of fluorescent probes T2 - SPIE BIOS 2013 - Part of Photonics West T2 - SPIE BIOS 2013 - Part of Photonics West CY - San Francisco, CA, USA DA - 2013-02-02 PY - 2013 AN - OPUS4-27856 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hennig, Andreas A1 - Behnke, Thomas A1 - Würth, Christian A1 - Hoffmann, Katrin A1 - Borcherding, H. A1 - Hoffmann, Angelika A1 - Jäger, Christian A1 - Schedler, U. T1 - Simple Spectroscopic Procedures for the Charcterization of the Application-Relevant Properties of Fluorescent Particles T2 - ANALYTICA 2012 T2 - ANALYTICA 2012 CY - Munich, Germany DA - 2012-04-17 PY - 2012 AN - OPUS4-24694 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - Kalibrierung von fluoreszenzbasierten Zellassays T2 - Senftenberger Innovationsforum T2 - Senftenberger Innovationsforum CY - Senftenberg, Germany DA - 2012-03-16 PY - 2012 AN - OPUS4-27521 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Kaiser, Martin A1 - Würth, Christian T1 - Luminescence Properties of Cer-dotierten Yttrium-Aluminium-Garnet (YAG:Ce) Nanoparticles T2 - Upcore Joint Seminar T2 - Upcore Joint Seminar CY - Regensburg, Germany DA - 2012-08-30 PY - 2012 AN - OPUS4-27522 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - Pauli, Jutta A1 - Rurack, Knut A1 - Grabolle, Markus T1 - From functional chromophores to fluorescent standards T2 - Seminar-Vortrag AK Prof. Eychmüller, Universität Dresden T2 - Seminar-Vortrag AK Prof. Eychmüller, Universität Dresden CY - Dresden, Germany DA - 2007-02-14 PY - 2007 AN - OPUS4-16681 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Pauli, Jutta T1 - Labels for fluorometric analysis T2 - Seminar-Vortrag, AK PD Alves, Universitätsklinikum Göttingen T2 - Seminar-Vortrag, AK PD Alves, Universitätsklinikum Göttingen CY - Göttingen, Germany DA - 2007-12-03 PY - 2007 AN - OPUS4-16682 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Grabolle, Markus A1 - Hoffmann, Katrin T1 - Labels and standards for fluorometric analysis T2 - Seminar-Vortrag, AK Prof. Weller, Universität Hamburg T2 - Seminar-Vortrag, AK Prof. Weller, Universität Hamburg CY - Hamburg, Germany DA - 2007-11-05 PY - 2007 AN - OPUS4-16683 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - Pauli, Jutta A1 - Rurack, Knut A1 - Grabolle, Markus A1 - Flachenecker, Günter T1 - Fluorescence spectroscopy at BAM: From functional chromophores to fluorescent standards T2 - Seminar-Vortrag, Novaled AG T2 - Seminar-Vortrag, Novaled AG CY - Dresden, Germany DA - 2007-10-02 PY - 2007 AN - OPUS4-16684 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - Traceability of and Requirements on Luminescence Standards: Development of a KIT Spectral Fluorescence Standards T2 - Fluka Productions GmbH T2 - Fluka Productions GmbH CY - Buchs, Switzerland DA - 2002-01-17 PY - 2002 AN - OPUS4-10900 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Pfeifer, Dietmar A1 - Pilz, Walter A1 - Monte, Christian A1 - Rurack, Knut A1 - Hoffmann, Angelika A1 - Spieles, Monika A1 - Taubert, D. A1 - Hollandt, J. T1 - EN ISO/IEC 17025 Imposed Requirements on Luminescence Measurements: Traceable Instrument Characterization, Recognized Recommendations, and Certified Fluorescence Standards T2 - Workshop on "Aqueous solutions of U(VI) as studied by time resolved laser-induced fluorescence spectroscopy (TRLFS): a round robin test” T2 - Workshop on "Aqueous solutions of U(VI) as studied by time resolved laser-induced fluorescence spectroscopy (TRLFS): a round robin test” CY - Strasbourg, France DA - 2002-01-24 PY - 2002 AN - OPUS4-10901 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - Proposal of a Pilot Study on Fluorometry: Spectral Correction of Emission and Excitation Spectra T2 - Annual CCQM Meeting T2 - Annual CCQM Meeting CY - Paris, France DA - 2002-04-25 PY - 2002 AN - OPUS4-10902 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - Pilot Study Spectral Correction of Emission Spectra T2 - Annual Meeting of the CCQM Bioanalysis Working Group T2 - Annual Meeting of the CCQM Bioanalysis Working Group CY - Paris, France DA - 2003-04-08 PY - 2003 AN - OPUS4-10904 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Pfeifer, Dietmar A1 - Pilz, Walter A1 - Monte, Christian A1 - Hoffmann, Angelika A1 - Spieles, Monika A1 - Taubert, D. A1 - Hollandt, J. T1 - Rückführbare spektrale Fluoreszenzstandards für die statische Fluorometrie T2 - Seminar Abteilung I, BAM T2 - Seminar Abteilung I, BAM CY - Berlin, Germany DA - 2002-10-21 PY - 2002 AN - OPUS4-10910 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - Pfeifer, Dietmar A1 - Monte, Christian A1 - Hoffmann, Angelika T1 - Fluorescence Standards Developed at BAM: Current and Future Activities T2 - IUPAC Task Force Group Fluorescence T2 - IUPAC Task Force Group Fluorescence CY - Lisbon, Portugal DA - 2005-09-04 PY - 2005 AN - OPUS4-10914 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Pfeifer, Dietmar A1 - Monte, Christian A1 - Hoffmann, Angelika A1 - Bremser, Wolfram A1 - Taubert, D. A1 - Hollandt, J. T1 - Linking fluormetry to radiometry T2 - National Physics Laboratory T2 - National Physics Laboratory CY - London, England DA - 2006-06-26 PY - 2006 AN - OPUS4-12591 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - Pauli, Jutta A1 - Grabolle, Markus T1 - From Functional Chromophores to Fluorescence Standards: Fluorescence Spectroscopy at BAM T2 - Institutionskolloquium Technische Universität München (TUM), Institut für Wasserchemie (Einladung Prof. Nießner) T2 - Institutionskolloquium Technische Universität München (TUM), Institut für Wasserchemie (Einladung Prof. Nießner) CY - Munich, Germany DA - 2008-12-08 PY - 2008 AN - OPUS4-18061 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Grabolle, Markus A1 - Kapusta, P. A1 - Koberling, F. T1 - Approaches to Lifetime Multiplexing with Quantum Dots and Organic Dyes: First Examples T2 - Kolloquium im Rahmen der FluoPlex-Treffen am Max-Planck-Institut für Molekulare Genetik T2 - Kolloquium im Rahmen der FluoPlex-Treffen am Max-Planck-Institut für Molekulare Genetik CY - Berlin, Germany DA - 2008-06-17 PY - 2008 AN - OPUS4-18062 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - DeRose, P. A1 - Smith, M. V. A1 - Duewer, D. L. A1 - Bremser, Wolfram A1 - Kramer, G. W. T1 - New Certified Reference Materials for Comparing Flourescence Spectra Between Laboratories T2 - Eastern Analytical Symposium T2 - Eastern Analytical Symposium CY - Somerset, NJ, USA DA - 2006-11-13 PY - 2006 AN - OPUS4-14328 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Angelika A1 - Pfeifer, Dietmar A1 - Hoffmann, Katrin A1 - Monte, Christian T1 - Standards for fluorescence analysis: From steady state fluorometry to fluorescence microscopy T2 - Fraunhofer Institut für Angewandte Polymerforschung T2 - Fraunhofer Institut für Angewandte Polymerforschung CY - Potsdam, Germany DA - 2006-02-02 PY - 2006 AN - OPUS4-14329 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - DeRose, P. A1 - Hoffmann, Angelika A1 - Bremser, Wolfram T1 - Standardization of fluorescence techniques: Where do we stand and what do we need? T2 - PittCon 2007 T2 - PittCon 2007 CY - Chicago, IL, USA DA - 2007-02-25 PY - 2007 AN - OPUS4-14695 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - DeRose, P. A1 - Smith, M. V. A1 - Duewer, D. L. A1 - Bremser, Wolfram A1 - Kramer, G. W. T1 - Comparability of Fluorescence Measurement: An International Study T2 - Intercomparison of Fluorescence PITTCON 2007 T2 - Intercomparison of Fluorescence PITTCON 2007 CY - Chicago, IL, USA DA - 2007-02-25 PY - 2007 AN - OPUS4-16413 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - Hoffmann, Angelika T1 - Standardization of fluorescence measurements: BAM approaches to fluorescence standards T2 - Osram GmbH T2 - Osram GmbH CY - Munich, Germany DA - 2008-04-24 PY - 2008 AN - OPUS4-17657 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Würth, Christian A1 - Hennig, Andreas A1 - Behnke, Thomas A1 - Hatami, Soheil A1 - Hoffmann, Angelika A1 - Huber, Alexandra A1 - Jäger, Christian T1 - Methods for the Determination of the Optical Properties and the Surface Chemistry of Fluorescent Particles T2 - SPIE BIOS 20123 - Part of Photonics West T2 - SPIE BIOS 20123 - Part of Photonics West CY - San Francisco, CA, USA DA - 2013-02-02 PY - 2013 AN - OPUS4-29229 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Würth, Christian A1 - Grabolle, Markus A1 - Pauli, Jutta A1 - Spieles, Monika T1 - Relative and Absolute Measurement of Photoluminescence Quantum Yields and Achievable Uncertainties T2 - PTB-Braunschweig T2 - PTB-Braunschweig CY - Braunschweig, Germany DA - 2013-11-06 PY - 2013 AN - OPUS4-29572 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Bremser, Wolfram A1 - Pfeifer, Dietmar A1 - Hoffmann, Angelika A1 - Spieles, Monika T1 - Comparability of Emission Spectra – Wavelength-dependent Uncertainties and Results from a First Interlaboratory Comparison T2 - PTB-Braunschweig T2 - PTB-Braunschweig CY - Braunschweig, Germany DA - 2013-11-06 PY - 2013 AN - OPUS4-29573 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Behnke, Thomas A1 - Würth, Christian A1 - Hennig, Andreas A1 - Pauli, Jutta A1 - Hoffmann, Katrin A1 - Hatami, Soheil A1 - Spieles, Monika A1 - Hoffmann, Angelika T1 - Design, Spectroscopic and Analytical Characterization of Functional Dye Systems for Applications in the Life Sciences and for Biosensing T2 - Projektmeeting T2 - Projektmeeting CY - Potsdam, Germany DA - 2013-11-20 PY - 2013 AN - OPUS4-29637 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - Fluorescence applications in molecular imaging: Labels and probes T2 - eingeladener Vortrag T2 - eingeladener Vortrag CY - Göttingen, Germany DA - 2009-07-15 PY - 2009 AN - OPUS4-19676 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - Pauli, Jutta A1 - Grabolle, Markus T1 - From Functional Chromophores to Fluorescence Standards T2 - Instituts-Seminar Universität Regensburg T2 - Instituts-Seminar Universität Regensburg CY - Regensburg, Germany DA - 2009-05-08 PY - 2009 AN - OPUS4-19675 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - Grabolle, Markus A1 - Brehm, Robert A1 - Behnke, Thomas A1 - Würth, Christian A1 - Spieles, Monika A1 - Linck, Lena A1 - Hoffmann, Angelika A1 - Pauli, Jutta A1 - Lochmann, Cornelia T1 - Functional Chromophores and Standardized Fluorescence Measurements: Fluorescence Applications at BAM T2 - Besuch Sigma-Aldrich GmbH T2 - Besuch Sigma-Aldrich GmbH CY - Buchs, Switzerland DA - 2009-08-21 PY - 2009 AN - OPUS4-20431 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Grabolle, Markus A1 - Behnke, Thomas A1 - Würth, Christian A1 - Hoffmann, Katrin A1 - Pauli, Jutta A1 - Brehm, Robert T1 - Functional Chromophores, Method development and fluorescence standards for fluorescence-based Bioanalysis T2 - TU Berlin, Fachbereich Pysik AK Prof. Hoffmann T2 - TU Berlin, Fachbereich Pysik AK Prof. Hoffmann CY - Berlin, Germany DA - 2011-01-03 PY - 2011 AN - OPUS4-23527 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Würth, Christian A1 - Grabolle, Markus A1 - Pauli, Jutta A1 - Lochmann, Cornelia A1 - Spieles, Monika T1 - Methods for the Determination of the Fluorometric Key Parameter Photoluminescence Quantum Yield - Relative and Absolute Measurement and Achievable Uncertainties T2 - TU Berlin, Fachbereich Pysik AK Prof. Hoffmann T2 - TU Berlin, Fachbereich Pysik AK Prof. Hoffmann CY - Berlin, Germany DA - 2011-03-01 PY - 2011 AN - OPUS4-23528 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Grabolle, Markus A1 - Behnke, Thomas A1 - Würth, Christian A1 - Hoffmann, Katrin A1 - Pauli, Jutta A1 - Brehm, Robert T1 - Functional Chromophores, Method development and fluorescence standards for fluorescence-based Bioanalysis T2 - eingeladener Vortrag FU Berlin T2 - eingeladener Vortrag FU Berlin CY - Berlin, Germany DA - 2011-02-08 PY - 2011 AN - OPUS4-23531 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Würth, Christian A1 - Grabolle, Markus A1 - Pauli, Jutta A1 - Lochmann, Cornelia A1 - Spieles, Monika T1 - Fluorometric methods for the measurement of photoluminescence quantum yields T2 - eingeladener Vortrag TU Berlin T2 - eingeladener Vortrag TU Berlin CY - Berlin, Germany DA - 2011-02-22 PY - 2011 AN - OPUS4-23533 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hennig, Andreas A1 - Hoffmann, Katrin A1 - Hoffmann, Angelika T1 - Simple Procedure for the Quantification of Surface Functionalities an Beads and Planar substrates T2 - Anakon 2011 T2 - Anakon 2011 CY - Zurich, Switzerland DA - 2011-03-22 PY - 2011 AN - OPUS4-23535 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - Würth, Christian A1 - Behnke, Thomas A1 - Hoffmann, Angelika T1 - Problem-adaptable multifunctional calibration tools for the traceable characterization of fluorescence measuring systems T2 - Analytica T2 - Analytica CY - Munich, Germany DA - 2010-03-23 PY - 2010 AN - OPUS4-21029 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Pauli, Jutta A1 - Behnke, Thomas A1 - Brehm, Robert A1 - Grabolle, Markus A1 - Hoffmann, Katrin A1 - Würth, Christian A1 - Mathejczyk, J. A1 - Alves, F. A1 - Hamann, J. A1 - Hilger, I. T1 - Dye-Biomolecule Conjugates and NIR-Fluorescent Particles for Targeting of Disease-related Biomarkers T2 - Eingeladener Vortrag SPIE Photonics West T2 - Eingeladener Vortrag SPIE Photonics West CY - San Francisco, CA, USA DA - 2011-01-22 PY - 2011 AN - OPUS4-22077 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hennig, Andreas A1 - Würth, Christian A1 - Hatami, Soheil A1 - Hoffmann, Angelika A1 - Hoffmann, Katrin A1 - Schedler, U. A1 - Borcherding, H. A1 - Huber, A. T1 - Dye-Biomolecule Conjugates and Fluorescent Particles - Spectroscopic Characterization and Application for Targeting of Disease-related Biomarkers T2 - Physikalisch-Chemisches Seminar T2 - Physikalisch-Chemisches Seminar CY - Hamburg, Germany DA - 2012-01-17 PY - 2012 AN - OPUS4-27024 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Würth, Christian A1 - Behnke, Thomas A1 - Hennig, Andreas A1 - Hatami, Soheil A1 - Hoffmann, Katrin A1 - Pauli, Jutta A1 - Spieles, Monika A1 - Hoffmann, Angelika T1 - Absolute fluorometry in the life and material sciences Characterization of NIR fluorophores, fluorescent particles and scattering and fluorescent systems T2 - Biosensor Symposium T2 - Biosensor Symposium CY - Wildau, Germany DA - 2013-03-10 PY - 2013 AN - OPUS4-29636 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hatami, Soheil A1 - Würth, Christian A1 - Kaiser, Martin A1 - Leubner, S. A1 - Graponik, N. A1 - Eychmüller, A. T1 - Measurement of Absolute Fluorescence Quantum Yields in the Near-Infrared and Infrared Spectral Region T2 - SPIE Photonics West 2014 (international society for optics and photonics) T2 - SPIE Photonics West 2014 (international society for optics and photonics) CY - San Francisco, CA, USA DA - 2014-02-01 PY - 2014 AN - OPUS4-30199 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hatami, Soheil A1 - Kaiser, Martin A1 - Würth, Christian A1 - Leubner, S. A1 - Moser, Marko A1 - Schneider, Ralf A1 - Gaponik, N. A1 - Eychmüller, A. T1 - Optical Properties and Surface Chemistry of Quantum Dots, Polymeric and Upconversion Nanoparticles T2 - SPIE Photonics West 2014 (international society for optics and photonics) T2 - SPIE Photonics West 2014 (international society for optics and photonics) CY - San Francisco, CA, USA DA - 2014-02-01 PY - 2014 AN - OPUS4-30200 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Nirmalananthan-Budau, Nithiya A1 - Tavernaro, Isabella T1 - Engineered Nanomaterials- Novel Approaches for Risk Assessment and Safe-by-Design N2 - The overall interest in nanotoxicity, triggered by the increasing use of nanomaterials in the material and life sciences, and the synthesis of an ever increasing number of new functional nanoparticles calls not only for standardized test procedures1,2 and for efficient approaches to screen the potential genotoxicity of these materials, but also for standardized and validated methods for surface analysis. 4,5 The analysis and quantification of surface chemistry is hence in the focus of an increasing number of standardization organizations and interlaboratory comparisons with different analytical methods are being done.5 For the monitoring of nanomaterial synthesis and the fast assessment of the number of functional groups such as carboxyl and amino functionalities, which are very commonly used functionalities in the life sciences, simple and validated methods are needed that can be performed with common laboratory instrumentation. 5,6 Here we provide a brief overview of the ongoing research in division Biophotonics employing quantitative NMR (qNMR), conductometry, and colorimetric and fluorometric optical assays for the determination of the total and the accessible number of carboxyl and amino groups on differently sized polymer and silica nano- and microparticles.5-7 T2 - Workshop NanoRiskSD project CY - Berlin, Germany DA - 09.06.2022 KW - Nanoparticle KW - Surface analysis KW - Standardization KW - Quality assurance KW - Validation KW - qNMR KW - Silica KW - Polymer KW - Fluorescence KW - Assay KW - Conductometry KW - Method comparison KW - Analytical sciences PY - 2022 AN - OPUS4-54999 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Tavernaro, Isabella A1 - Nirmalananthan-Budau, Nithiya T1 - Functional molecular chromophores and nanomaterials reporters, optical probes, and sensors N2 - •Overview of expertise of division Biophotonics •Functional molecular and nanoscale luminescent reporters, probes, and sensors including design principles available from division Biophotonics •Examples for pH and O2 responsive molecular probes and particle sensors •Surface group quantification Analytical methods and cleavable probes •Concepts & standards for the validation and traceability of optical measurements T2 - FU-Seminar CY - Berlin, Germany DA - 09.12.2022 KW - Particle sensors KW - Surface group quantification KW - Nanoscale luminescent reporters KW - Cleavable probes PY - 2022 AN - OPUS4-56526 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Frenzel, Florian A1 - Würth, Christian A1 - Grauel, Bettina A1 - Hirsch, T. A1 - Haase, M. T1 - Measuring the Upconversion Luminescence of Ensemble and Single Particle Lanthanide-Based Upconversion Nanocrystals N2 - Lanthanide-based upconversion nanoparticles (UCNPs) like hexagonal 𝛽-NaYF4 UCNPs doped with Yb3+ and Er3+, which efficiently convert 976 nm light to ultraviolet, visible, and near infrared photons, offer new strategies for luminescence-based sensing, barcoding, and Imaging. Their upconversion (UC) luminescence (UCL) features like UCL intensity, quantum yield, relative spectral distribution / UCL luminescence color, and luminescence decay kinetics are, however, strongly influenced by particle size, dopant ion concentration, particle architecture, surface chemistry including presence and thickness of surface passivation and shielding shells, microenvironment/presence of quenchers with high energy vibrations, and excitation power density (P). We present here a comprehensive study of the influence of excitation power density on the UCL features of different types of UCNPs, focusing on Yb3+ and Er3+ co-doped NaYF4 core-only and core-shell nanostructures with different sizes and doping ion concentration, which underlines the importance of P-dependent optimum dopant concentrations for UCNP performance and the potential of P-tuning of UCL. T2 - Materials Challenges in Alternative & Renewable Energy 2021 (MCARE 2021) CY - Online meeting DA - 19.07.2021 KW - Fluorescence KW - Lifetime KW - Method KW - Stability KW - Coating KW - Surface chemistry KW - Lanthanide KW - Upconversion KW - Nano KW - Particle KW - Single particle spectroscopy KW - Quantum yield KW - Microscopy KW - Photophysics PY - 2021 AN - OPUS4-53111 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - Standardization of fluorescence measurements: Design, development, validation, and fabrication of format-adaptable fluorescence standards for intensity, spectral, and temporal quantities N2 - Photoluminescence techniques are amongst the most widely used tools in the material and life sciences, with new and exciting applications continuously emerging, due to their many advantages like comparative ease of use, unique sensitivity, non-invasive character, and potential for multiplexing, remote sensing, and miniaturization. Drawbacks are , however, signals, that contain unwanted wavelength- and polarization contributions from instrument-dependent effects, which are time-dependent due to the aging of instrument components, and difficulties to measure absolute fluorescence intensities. Thus, there is a considerable need for standards for intensity, spectral, and temporal fluorescence quantities to meet the increasing need for instrument performance validation and global trends to harmonize physicochemical measurements. In this respect, instrument calibration strategies together with different types of fluorescence standards are presented as well as design concepts for robust, easy-to-use, and format-adaptable fluorescence standards useable for the determination of different fluorescence parameters and a broad variety of fluorescence techniques. T2 - SALSA-Kolloquien CY - Berlin, Germany DA - 07.06.2016 KW - Fluorescence KW - Standard KW - Quality assurance KW - Spectral correction KW - Quantum yield PY - 2016 AN - OPUS4-37070 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Behnke, Thomas A1 - Moser, Marko A1 - Quevedo, Pablo A1 - Nirmalananthan-Budau, Nithiya A1 - Weigert, Florian A1 - Würth, Christian A1 - Hoffmann, Katrin A1 - Ermilov, Eugeny A1 - Pauli, Jutta T1 - Simple methods, validation concepts, and reference materials for the characterization of functional nanomaterials and microparticles N2 - The surface chemistry / functionalization of nanomaterials and microparticles largely controls the stability of these materials as well as their solubility and subsequent biofunctionalization and their interactions with biological systems. Moreover, in the case of some nanomaterials like semiconductor quantum dots or lanthanide-based upconversion nanocrystals, the ligand shell strongly affects their optical properties, e.g., via passivation of surface states and traps that favor luminescence quenching or the protection of surface atoms from quenching water molecules. This renders analytical methods for the quantification of surface groups like functionalities very important. Targets of broad interest are here amino, carboxyl, alkine and maleimide groups used for common bioconjugation reactions and typical ligands like thiols and polyethylene glycol (PEG) molecules of varying length, used for the tuning of material hydrophilicity and biocompatibility, minimization of unspecific interactions, prevention of biofouling, and enhancement of blood circulation times as well as surface-bound biomolecules like streptavidin or other biomolecules relevant e.g., for diagnostic assays. Here, we focus on simple optical methods relying on standard laboratory instrumentation, validated by method comparison and/or mass balances and present examples for their use for the characterization of different types of nanomaterials and microparticles. T2 - Innovationsforum Senftenberg CY - Senftenberg, Germany DA - 01.06.2016 KW - Surface chemistry KW - Functional group analysis KW - Thiol assay KW - Fluorescence KW - Nanomaterial KW - Nanoparticle KW - PEG KW - Ligand KW - Semiconductor quantum dot KW - Quantum yield KW - Quantification KW - Method validation KW - Integrating sphere spectroscopy KW - Fluorescence standard PY - 2016 N1 - Geburtsname von Nirmalananthan-Budau, Nithiya: Nirmalananthan, N. - Birth name of Nirmalananthan-Budau, Nithiya: Nirmalananthan, N. AN - OPUS4-37111 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - Applications and challenges of luminescence-based detection methods in the life and material sciences N2 - Luminescence-based detection methods, ranging from fluorescence spectroscopy for photophysical and mechanistic studies over sensing applications, chromatographic separation techniques and the microarray technology with fluorescence detection to fluorescence microscopy, flow cytometry, single molecule spectroscopy, and molecular imaging to integrating sphere spectroscopy, are among the most widely used methods in the life and material sciences. This is due to e.g., their unique sensitivity enabling the detection of single molecules, potential for multiplexing, ease of combination with spatial resolution, and suitability for remote sensing. Many of these advantages are closely linked to the choice of suitable molecular and nanoscale fluorescent reporters, typically required for signal generation. This includes organic dyes without and with sensor function, fluorophore-encoded polymeric and silica nanoparticles as well as nanocrystalline systems like semiconductor quantum dots and upconversion phosphors, emitting in the visible (vis), near-infrared (NIR), and IR (infrared). Current challenges present the environment sensitivity of most fluorophores, rendering fluorescence spectra, measured intensities/fluorescence quantum yields, and fluorescence decay kinetics matrix-dependent, and instrument-specific distortions of measured fluorescence signals that need to be considered for quantification and comparability of data, particularly fluorescence spectra. Here, current applications of luminescence-based methods and different types of reporters will be presented. In this context, suitable spectroscopic tools for the characteri-zation of the optical properties of fluorescent reporters and fluorophore-encoded microparticles, analytical tools for the determination of the surface chemistry of different types of particles, and different multiplexing strategies will be discussed. T2 - 9th Meeting of Engineering of Functional Interfaces CY - Wildau,Germany DA - 03.07.2016 KW - Fluorescence KW - Multiplexing KW - Lifetime KW - Nanomaterial KW - Nanoparticle KW - PEG KW - Ligand KW - Semiconductor quantum dot KW - Quantum yield KW - Quantification KW - Upconversion nanoparticle KW - Integrating sphere spectroscopy KW - NIR KW - IR KW - Fluorescence standard KW - Calibration PY - 2016 AN - OPUS4-37112 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Moser, Marko A1 - Kraft, Marco A1 - Würth, Christian A1 - Behnke, Thomas A1 - Falkenhagen, Jana A1 - Kaiser, Martin A1 - Muhr, V. A1 - Hirsch, T. T1 - Simple method for the quantification of PEG ligands and guidelines to the spectroscopic characterization of upconversion nanoparticles N2 - The surface modification of nanometer- and micrometer-sized particles with polyethylene glycol (PEG) ligands of varying length is a very common strategy to tune their hydrophilicity and biocompatibility, minimize unspecific interactions, improve biofunctionalization efficiencies, and enhance blood circulation times. Nevertheless, simple methods for the quantification of PEG ligands are rare. This is similarly true for the spectroscopic characterization of lanthanide-doped upconverting nanoparticles (UCNPs), novel near infrared (NIR)-excitable nonlinear fluorescence reporters for bioanalysis and theranostics, providing background-free multiple narrow emission bands in the visible and NIR, excellent photostability, and long luminescence lifetimes. We present here a simple method for the determination of the number of PEG ligands on nanomaterials and screening methods for the fast identification of nonradiative deaction pathways in UCNPs. T2 - SPIE Photonics West CY - San Francisco, CA, USA DA - 15.02.2016 KW - Optical assay KW - Fluorescence KW - Integrating sphere spectroscopy KW - Quantum yield KW - NIR KW - IR KW - Optical spectroscopy KW - Nanomaterials KW - Semiconducor nanocrystals KW - Upconversion nanocrystals KW - Surface analysis; KW - Ligand analysis PY - 2016 AN - OPUS4-35951 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hatami, Soheil A1 - Würth, Christian A1 - Kaiser, Martin A1 - Leubner, S. A1 - Gaponik, N. A1 - Eychmüller, A. T1 - Absolute fluorescence measurements > 1000 nm - Setup design, calibration and standards N2 - There is an increasing interest in optical reporters like semiconductor quantum dots and upconversion nanophosphors with emission > 1000 nm for bioanalysis, medical diagnostics, and safety barcodes and hence, in reliable fluorescence measurements in this wavelength region, e.g., for the comparison of material performance and the rational design of new nanomaterials with improved properties. Here, we present the design of an integrating sphere setup for the absolute measurement of emission spectra and quantum yields in the wavelength region of 650 to 1600 nm and its calibration as well as examples for potential fluorescence standards from different reporter classes for the control of the reliability of such measurements. T2 - SPIE Photonics West 2016 CY - San Francisco, CA, USA DA - 15.02.2016 KW - IR fluorescence KW - Quantum dot KW - Upconversion nanocrystal KW - Integrating sphere spectroscopy KW - Absolute fluorescence quantum yield KW - Nanophosphor KW - Calibration PY - 2016 AN - OPUS4-35953 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Würth, Christian A1 - Behnke, Thomas A1 - Moser, Marko A1 - Schneider, Ralf A1 - Kraft, Marco A1 - Pauli, Jutta A1 - Kaiser, Martin A1 - Güttler, Arne T1 - Methods for the determination of the optical properties and the surface chemistry of fluorescent particles N2 - Fluorescent particles like nm- and m-sized polymeric beads doped or labeled with different types of fluorophores and nanocrystalline systems like quantum dots and upconversion phosphors emitting in the visible (vis), near-infrared (NIR), and IR (infrared) region are of increasing importance as fluorescent reporters for bioanalysis and medical diagnostics. The assessment and comparison of material performance and the development of rational design strategies for improved systems requires suitable spectroscopic tools for the determination of signal-relevant optical properties and analytical tools for the determination of the number of surface groups, ligands, biomolecules and /or fluorophores per bead. In this respect, suitable spectroscopic tools for the characterization of the optical properties of such materials like photoluminescence quantum yields and brightness values and the determination of their surface chemistry are introduced. This includes integrating sphere setups for absolute measurements of fluorescence quantum yields of liquid and solid, transparent and scattering materials in the wavelength region of 350 nm to 1600 nm at varying excitation power densities for the study of multi-photon processes and simple optical assays, validated by comparison with established analytical techniques relying on different detection principles. Here, different examples for the optical and analytical characterization of different types of nanoscale reporters are presented. T2 - MoLife Research Seminar CY - Bremen, Germany DA - 26.04.2016 KW - Optical assay KW - Fluorescence KW - Integrating sphere spectroscopy KW - Quantum yield KW - NIR KW - IR KW - Optical spectroscopy KW - Nanomaterials KW - Semiconducor nanocrystals KW - Upconversion nanocrystals KW - Surface analysis KW - Ligand analysis PY - 2016 AN - OPUS4-35954 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Behnke, Thomas A1 - Moser, Marko A1 - Quevedo, Pablo A1 - Würth, Christian A1 - Krüger, Harald A1 - Nirmalanathan, Nithiya A1 - Pauli, Jutta A1 - Hoffmann, Katrin T1 - Design, characterization and application of molecular and nanoscale optical reporters and probes and method standardization N2 - Here, we present an overview of the research activities of division Biophotonics concerning the design and (bio)analytical application of molecular and nanoscale functional chromophores for e.g., biomarker analysis including methods for the reliable determination of their application relevant properties. This includes the absolute determination of their brightness and photoluminescemce quantum yield, determining the signal size from the material side, as well as the development of fluorescence standards for such measurements and instrument calibration strategies, thereby providing important prerequisites for the comparison of material performance, the mechanistic understanding of nonradiative decay channels, and the rational design of new optical reporters. Moreover, for particle-based systems, ranging from nm-sized semiconductor quantum dots to m-sized polymeric and silica beads, simple optical methods and assays for the assessment of their surface chemistry are presented, which enable the quantification of the number of total and derivatizable surface functionalities, ligands per particle and particle-bound biomolecules. In addition, validation concepts for such methods are introduced utilizing method comparisons, multimodal and cleavable probes. T2 - Seminar Microbiology of Interfaces CY - Magdeburg, Germany DA - 16.06.2016 KW - Biomarker PY - 2016 AN - OPUS4-36997 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Würth, Christian A1 - Kaiser, Michael A1 - Schneider, Rudolf A1 - Kraft, Marco A1 - Leubner, S. A1 - Gaponik, N. A1 - Eychmüller, A. A1 - Wilhelm, Stefan A1 - Hirsch, T. T1 - Characterization of nanocrystalline particles with NIR emission - Spectroscopic properties and surface group analysis N2 - There is an increasing interest in optical reporters like semiconductor quantum dots and upconversion nanocrystals with emission > 800 nm for bioanalysis, medical diagnostics, and safety barcodes. Prerequisites for the comparison of material performance, the mechanistic understanding of nonradiative decay channels, and the rational design of new nanomaterials with improved properties are reliable fluorescence measurements and validated methods for the assessment of their surface chemistry. The latter is of special relevance for nanocrystalline emitters, where surface states and the accessibility of emissive states by quenchers largely control accomplishable photoluminescence quantum yields and hence, signal sizes and detection sensitivities from the reporter side. Here, we present the design of integrating sphere setups for the excitation power density-dependent absolute measurement of emission spectra and photoluminescence quantum yields in the wavelength region of 350 to 1600 nm and results from spectroscopic studies of semiconductor quantum dots and upconversion nanocrystals of different size and surface chemistries in various environments. Subsequently, examples for simple approaches to surface group and ligand analysis are presented. T2 - 1st International Biophotonics Conference CY - Singapure DA - 25.07.2016 KW - Fluorescence KW - Integrating sphere spectroscopy KW - Quantum yield KW - NIR KW - IR KW - Optical spectroscopy KW - Nanomaterials KW - Semiconducor nanocrystals KW - Surface analysis KW - Ligand analysis KW - Optical assay PY - 2016 AN - OPUS4-36998 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - Würth, Christian A1 - Hoffmann, Angelika T1 - Method-adaptable Fluorescence Standarts as Flexible Calibration Tools for the Standardization of Fluorescence-Based Measurements T2 - SPIE Biodefense T2 - SPIE Biodefense CY - Orlando, FL, USA DA - 2010-04-05 PY - 2010 AN - OPUS4-21546 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Angelika A1 - Hoffmann, Katrin T1 - Fluorescence Standards at BAM - Strategies for the Design, Characterization and Validation of Calibration Tools for the Standartization of Fluorescence Measurements T2 - Workshop zur Kalibrierung von Microarray-Scannern T2 - Workshop zur Kalibrierung von Microarray-Scannern CY - Berlin, Germany DA - 2010-02-26 PY - 2010 AN - OPUS4-21547 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute T1 - Labels and Probes For Molecular Imaging: Design Principles, Spectroscopic Characterization and Current Research Activities at BAM T2 - Eingeladener Vortrag T2 - Eingeladener Vortrag CY - Göttingen, Germany DA - 2010-05-31 PY - 2010 AN - OPUS4-21542 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Pauli, Jutta T1 - Characterization and evaluation of a tumor specific RGD optical probe for timedomain near-infrared fluorescence imaging T2 - 5th European Molecular Imaging Meeting T2 - 5th European Molecular Imaging Meeting CY - Warsaw, Poland DA - 2010-05-26 PY - 2010 AN - OPUS4-21543 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Pauli, Jutta A1 - Grabolle, Markus A1 - Brehm, Robert A1 - Hennig, Andreas A1 - Würth, Christian A1 - Hoffmann, Katrin A1 - Behnke, Thomas T1 - Molecular and Particulate Labels and Probes for Optical Imaging T2 - Exzellenzakademie Medizintechnik: Krankheitsspezifische & molekulare Bildgebung T2 - Exzellenzakademie Medizintechnik: Krankheitsspezifische & molekulare Bildgebung CY - Aachen, Germany DA - 2010-04-26 PY - 2010 AN - OPUS4-21544 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - Pauli, Jutta A1 - Grabolle, Markus T1 - Applications of Fluorescence Spectroscopy: From Functional Chromophores to Fluorescence Standards T2 - Fluoreszenz-Kolloquium von ISS T2 - Fluoreszenz-Kolloquium von ISS CY - Regensburg, Germany DA - 2009-12-08 PY - 2009 AN - OPUS4-19422 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Behnke, Thomas A1 - Würth, Christian A1 - Pauli, Jutta A1 - Hoffmann, Katrin A1 - Grabolle, Markus A1 - Hennig, Andreas A1 - Brehm, Robert A1 - Spieles, Monika T1 - Functional Chromophores, Signal Enhancemant and Multiplexing Strategies and Absolute Fluorescence Measurement T2 - eingeladender Vortrag, Seminar AK Enderlein und Laserlabor Göttingen T2 - eingeladender Vortrag, Seminar AK Enderlein und Laserlabor Göttingen CY - Göttingen, Germany DA - 2010-11-24 PY - 2010 AN - OPUS4-22658 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Behnke, Thomas A1 - Würth, Christian A1 - Hoffmann, Katrin A1 - Grabolle, Markus A1 - Lochmann, Cornelia T1 - Design of Fluorescent (Nano)Particles - Specroscopic Characterization and Application for Biomarker Analysis T2 - Optical Imaging Workshop T2 - Optical Imaging Workshop CY - Göttingen, Germany DA - 2010-11-25 PY - 2010 AN - OPUS4-22660 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Behnke, Thomas A1 - Würth, Christian A1 - Hoffmann, Katrin A1 - Hennig, Andreas A1 - Pauli, Jutta A1 - Grabolle, Markus A1 - Lochmann, Cornelia A1 - Brehm, Robert T1 - From Funcional Chromophores to Fluorescence Standards: Fluorescence Spectroscopy at BAM T2 - Institutskolloquium T2 - Institutskolloquium CY - Münster, Germany DA - 2010-10-20 PY - 2010 AN - OPUS4-22662 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Würth, Christian A1 - Behnke, Thomas A1 - Moser, Marko A1 - Schneider, Ralf A1 - Kraft, Marco A1 - Kaiser, Martin A1 - Hatami, Soheil T1 - Methods for the determination of the optical properties and the surface chemistry of fluorescent particles T2 - 11th Internatinal Biosensor Conference BBMEC T2 - 11th Internatinal Biosensor Conference BBMEC CY - Regensburg DA - 2015-09-28 PY - 2015 AN - OPUS4-34490 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Quevedo, Pablo A1 - Geißler, Daniel A1 - Würth, Christian A1 - Schneider, Ralf A1 - Moser, Marko A1 - Gaponik, N. A1 - Eychmüller, A. A1 - Leubner, S. A1 - Kaiser, Martin A1 - Hatami, Soheil T1 - Measuring Optical Properties and Surface Chemistry of Polymeric and Silica Nanoparticles and Semiconductor and Upconversion Nanocrystals T2 - NPL-Workshop T2 - NPL-Workshop CY - Peckforton Castle, Cheshire, UK DA - 2015-04-20 PY - 2015 AN - OPUS4-34049 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Würth, Christian A1 - Hatami, Soheil A1 - Leubner, S. A1 - Gaponik, N. A1 - Eychmüller, A. T1 - Absolute spectroscopic characterization of the optical properties of semiconductor and upconversion nanocrystals in the vis and IR N2 - Nanocrystalline fluorophores like semiconductor quantum dots and rods and recently also lanthanide-based upconversion phosphors with emission in the visible (vis), near-infrared (NIR), and IR (infrared) region are increasingly being used in bioimaging studies and fluorescence assays as well as in photovoltaics and solid state lighting. The assessment and comparison of material performance as well as the development of rational design strategies for improved systems require spectroscopic tools, which enable the determination of the signal-relevant optical properties like photoluminescence quantum yields and brightness values. In the case of nonlinear fluorescence as shown by upconversion materials, such measurements must be also performed as function of excitation power density. In this work, we report on methods for the absolute determination of the photoluminescence quantum yield and brightness of fluorescent particles in dispersion and as powders based on integrating sphere spectroscopy and underline the importance of such measurements for the understanding of the photophysics of such nanocrystals. T2 - International Conference on Fundamental Processes in Semiconductor Nanocrystals (FQDots16) CY - Berlin, Germany DA - 05.09.2016 KW - Fluorescence KW - Nanoparticle KW - Semiconductor quantum dot KW - Upconversion nanocrystal KW - NIR KW - Integrating sphere spectroscopy KW - Fluorescence quantum yield KW - Method PY - 2016 AN - OPUS4-38695 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Ermilov, Eugeny A1 - Hoffmann, Katrin T1 - Suitable geometries for the measurement of photoluminescence quantum yields of luminescent and scattering samples – The DIN TNS project N2 - The characterization of the optical properties of photoluminescent systems, that scatter, like dispersions of nanoparticles with sizes exceeding about 25 nm or solid nanophosphors is of increasing importance for many applications in the life and material sciences. Examples present nanoscale optical reporters and dye-doped microparticles for bioimaging, fluorescence assays or DNA sequencing as well as nanocrystalline emitters like semiconductor quantum dots and rods or lanthanide-based nanophosphors embedded into solid matrices for solid state lighting, display technologies, or barcoding/security applications. The assessment and comparison of material performance as well as the development of rational design strategies for improved systems require spectroscopic tools, which enable the determination of the signal-relevant optical properties like photoluminescence quantum yields and brightness values. This encouraged us to built up an integrating sphere setup enabling absolute measurements of photoluminescence spectra and quantum yields of transparent and scattering photoluminescent dispersions and solid samples in different measurement geometries, i.e., direct and indirect illumination and the combination of both geometries and perform first measurements with selected emitters. Here, the design of this setup is presented and first recommendations concerning suitable measurement geometries are given. T2 - DKE-Sitzung CY - Frankfurt am Main, Germany DA - 31.08.2016 KW - Nanoparticle KW - Integrating sphere KW - Fluorescence KW - Quantum yield KW - Method KW - Standardization KW - Calibration KW - Reference material PY - 2016 AN - OPUS4-38643 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Pauli, Jutta T1 - Characterization of dye-protein conjugates with optical methods N2 - Fluorophore labeled proteins and antibodies, referred to also as targeted optical probes, present a promising strategy for a variety of applications from fundamental cell-based biological studies to in vivo diagnostics and image guided surgeries in humans. In this respect, design strategies for the preparation of such conjugates from different dyes including analyte-responsive fluorophores are presented as well as their analytical and spectroscopic characterization employing Absorption spectroscopy and steady state and time-resolved fluorometry. Special emphasis is dedicated to the influence of dye hydrophilicity and labeling density on the optical properties and binding behavior of these dye-bioconjugates including their performance in in vitro and in vivo bioimaging studies. T2 - International Workshop “Affinity - Mass spectrometry – New Methods and Application to Protein Therapeutics Development” CY - Rüsselsheim am Main, Germany DA - 14.11.2016 KW - Fluorescence KW - Dye bioconjugates KW - Protein KW - Immunoassay KW - Quality assurance KW - Optical characterization KW - Dye KW - NIR KW - Imaging PY - 2016 AN - OPUS4-38504 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - Pauli, Jutta A1 - Behnke, Thomas A1 - Würth, Christian T1 - Standardization of Fluorescence Measurements in the UV/vis/NIR/IR - Needs for and requirements on calibration tools N2 - Photoluminescence techniques are amongst the most widely used tools in the life sciences, with new and exciting applications in medical diagnostics and molecular imaging continuously emerging. Advantages include their comparative ease of use, unique sensitivity, non-invasive character, and potential for multiplexing, remote sensing, and miniaturization. General drawbacks are, however, signals, that contain unwanted wavelength- and polarization contributions from instrument-dependent effects, which are also time-dependent due to aging of instrument-components, and difficulties to measure absolute fluorescence intensities. Moreover, scattering systems require special measurement geometries and the interest in new optical reporters with emission > 1000 nm strategies for reliable measurements in the second diagnostic for the comparison of material performance and the rational design of new fluorophores with improved properties. Here, we present strategies to versatile method-adaptable liquid and solid fluorescence standards for different fluorescence parameters including traceable instrument calibration procedures and the design of integrating sphere setups for the absolute measurement of emission spectra and quantum yields in the wavelength region of 350 to 1600 nm. Examples are multi-emitter glasses, spectral fluorescence standards, and quantum yield standards for the UV/vis/NIR T2 - Spie Photonics west 2017 CY - San Francisco, USA DA - 28.01.2017 KW - Fluorescence standard KW - Instrument calibration KW - Integrating sphere spectroscopy KW - Absolute fluorescence quantum yield KW - Fluorescent glasses KW - Nanoparticles PY - 2017 AN - OPUS4-39074 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Kraft, Marko A1 - Würth, Christian A1 - Kaiser, Martin A1 - Muhr, V. A1 - Hirsch, T. T1 - Recent insights in the spectroscopic properties of upconversion nanoparticles N2 - Lanthanide-doped up-converting nanoparticles (UCNPs) are promising reporters for medical diagnostics and bioimaging, which are excited in the near infrared (NIR) by multiphoton absorption processes, and show multiple narrow emission bands in the visible (vis) and NIR, long luminescence lifetimes in the μs range, and excellent photostability. Current limitations present their relative low absorption cross sections and low fluorescence efficiencies, with the latter being affected by particle size, surface chemistry, and microenvironment, particularly water. Here, we present results from systematic studies of the excitation power density dependent upconversion luminescence spectra, intensities/intensity ratios of the individual emission bands, slope factors, and quantum yields of UCNPs of varying size, dopant concentration, and surface chemistry in different microenvironments as well as the up- and downconversion luminescence decay kinetics of the different emission bands. Moreover, first studies of the energy transfer from UCNPs to surface-bound organic dyes acting as fluorescence acceptors are shown. Based upon these measurements, fluorescence deactivation channels are identified and spectroscopic parameters for the screening of material performance are derived. T2 - Spie Photonics west 2017 CY - San Francisco, USA DA - 28.01.2017 KW - Upconverting nanoparticles KW - Size KW - FRET KW - Fluorescence KW - Absolute fluorescence quantum yield KW - Fluorescence decay kinetics KW - Power density dependence PY - 2017 AN - OPUS4-39075 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Würth, Christian A1 - Kraft, Marko A1 - Kaiser, Martin A1 - Muhr, V. A1 - Hirsch, T. T1 - Spectroscopic properties of upconversion nanoparticles N2 - Lanthanide-doped up-converting nanoparticles (UCNPs) are promising reporters for medical diagnostics and bioimaging. Current limitations present their relative low absorption cross sections and low fluorescence efficiencies, with the latter being affected by particle size, surface chemistry, and microenvironment, particularly water. Here, we present results from systematic studies of the excitation power density dependent upconversion luminescence spectra, intensities/intensity ratios of the individual emission bands, slope factors, and quantum yields of UCNPs of varying size, dopant concentration, and surface chemistry in different microenvironments as well as the up- and downconversion luminescence decay kinetics of the different emission bands. Moreover, first studies of the energy transfer from UCNPs to surface-bound organic dyes acting as fluorescence acceptors are shown. Based upon these measurements, fluorescence deactivation channels are identified and spectroscopic parameters for the screening of material performance are derived. T2 - Projekttreffen COST CY - Straßbourg, France DA - 09.01.2017 KW - Upconverting nanoparticles KW - Size KW - FRET KW - Fluorescence KW - Absolute fluorescence quantum yield KW - Fluorescence decay kinetics KW - Power density dependence PY - 2017 AN - OPUS4-39076 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Hoffmann, Katrin A1 - Wegmann, Marc A1 - Hannemann, M. A1 - Somma, V. A1 - Jochum, T. A1 - Niehaus, J. A1 - Roggenbuck, D. T1 - Automated determination of genotoxicity of nanoparticles with DNA-based optical assays - The NANOGENOTOX project N2 - The overall interest in nanotoxicity, triggered by the increasing use of nanomaterials in the material and life sciences, and the synthesis of an ever increasing number of new functional nanoparticles calls for standardized test procedures1,2 and for efficient approaches to screen the potential genotoxicity of these materials. Aiming at the development of fast and easy to use, automated microscopic methods for the determination of the genotoxicity of different types of nanoparticles, we assess the potential of the fluorometric γH2AX assay for this purpose. This assay, which can be run on an automated microscopic detection system, relies on the detection of DNA double strand breaks as a sign for genotoxicity3. Here, we provide first results obtained with broadly used nanomaterials like CdSe/CdS and InP/ZnS quantum dots as well as iron oxide, gold, and polymer particles of different surface chemistry with previously tested colloidal stability and different cell lines like Hep-2 and 8E11 cells, which reveal a dependence of the genotoxicity on the chemical composition as well as the surface chemistry of these nanomaterials. These studies will be also used to establish nanomaterials as positive and negative genotoxicity controls or standards for assay performance validation for users of this fluorometric genotoxicity assay. In the future, after proper validation, this microscopic platform technology will be expanded to other typical toxicity assays. T2 - SPIE 2018 CY - San Francisco, USA DA - 27.01.2018 KW - Nanoparticle KW - Fluorescence KW - Surface chemistry KW - Size KW - Assay KW - Microscopy KW - Nanotoxicity KW - Toxicity KW - Automation KW - Calibration KW - Standard PY - 2018 AN - OPUS4-44186 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Würth, Christian A1 - Kraft, Marco A1 - Kaiser, Martin A1 - Radunz, Sebastian A1 - Muhr, V. A1 - Hirsch, T. T1 - Effect of particle architecture, dopant concentration, size, and excitation power density on the luminescence efficiency of upconversion nanocrystals N2 - Lanthanide-based upconversion nanoparticles (UCNPs) offer new strategies for luminescence-based sensing and imaging. One of the best studied materials are hexagonal ß-NaYF4 UCNPs doped with 20% Yb3+ and 2% Er3+, which efficiently convert 976 nm light to photons emitted at 540 nm, 655 nm, and 845 nm, respectively, reveal Long luminescence lifetimes (> 100 µs), and are very photostable and chemically inters.[1,2] The properties of their upconversion (UC) luminescence (UCL) are, however, strongly influenced by particle size, concentration and spatial arrangement of dopant Ions, surface chemistry, and microenvironment.[3,4] In addition, the multiphotonic absorption processes responsible for UCL render UCL dependent on excitation power density (P). The rational design of brighter UCNPs particle architectures encouraged us to assess systematically the influence of these parameters on UCL for differently doped UCNPs relying on the commonly used ß-NaYf4 matrix using steady state and time resolved fluorometry as well as integrating sphere spectroscopy for P varied over almost three orders of magnitude. This includes comprehensive studies of the influence of size and shell, Yb3+ and Er3+ dopand concentrations, and energy Transfer processes from UCNPs to surface-bound organic dyes or vice versa [5]. Our results underline the need for really quantitative luminescence studies for mechanistic insights, the potential of high p to compensate for UCL surface quenching, and the matrix- and P-dependence of the optimum dopand concentration. T2 - BIOSPIE 2018 CY - San Francisco, USA DA - 27.01.2018 KW - Upconversion KW - Nanoparticle KW - Absolute fluorometry KW - Integrating sphere spectroscopy KW - NIR KW - IR KW - Fluorescence KW - Surface chemistry KW - Deactivation pathways KW - Modeling KW - Size PY - 2018 AN - OPUS4-43939 LA - deu AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Wuerth, Christian A1 - Kraft, Marco A1 - Kaiser, Martin A1 - Radunz, Sebastian A1 - Muhr, V. A1 - Hirsch, Th. A1 - Soukka, T. A1 - Homann, C. A1 - Haase, M. T1 - Luminescence efficiency of upconversion – Quantification of size, particle architecture and dopant concentration effects for different excitation power densities N2 - Lanthanide-based upconversion nanoparticles (UCNPs)like hexagonal beta-NaYF4 UCNPs doped with Yb3+ and Er3+, which efficiently convert 976 nm light to ultraviolet, visible, and near infrared photons, offer new strategies for luminescence-based sensing and imaging. The properties of their upconversion (UC) luminescence (UCL) are, however, strongly influenced by particle size, concentration and spatial arrangement of dopant ions, surface chemistry, microenvironment, and excitation power density (P). We present here comprehensive studies of the influence of UCNP size and particle architecture for Yb3+ and Er3+ co-doped NaYF4 nanoparticles and energy transfer processes in these materials, which underline the importance of quantitative luminescence measurements for mechanistic insights and the determination of application-relevant matrix- and P-dependent optimum dopand concentrations. T2 - 2nd Conference and Spring School CY - Valencia, Spain DA - 04.04.2018 KW - Upconverting nanoparticles KW - Size KW - Energy transfer KW - Fluorescence KW - Absolute fluorescence quantum yield KW - Fluorescence decay kinetics KW - Power density dependence PY - 2018 AN - OPUS4-44960 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Nirmalananthan-Budau, Nithiya A1 - Moser, Marko A1 - Geißsler, Daniel A1 - Behnke, Thomas A1 - Schneider, Ralf T1 - Simple and versatile methods for quantifying functional groups, ligands, and biomolecules on nanomaterials N2 - Many applications of nanomaterials in the life sciences require the controlled functionalization of these materials with ligands like polyethylene glycol (PEG) and/or biomolecules such as peptides, proteins, and DNA. This enables to tune their hydrophilicity and biocompatibility, minimize unspecific interactions, improve biofunction-nalization efficiencies, and enhance blood circulation times and is the ultimate prerequisite for their use as reporters in assays or the design of targeted optial probes for bioimaging. At the core of these functionalization strategies are reliable and validated methods for surface group and ligand quantification that can be preferably performed with routine laboratory instrumentation, require only small amounts of substances, and are suitable for many different types of nanomaterials. We present here versatile and simple concepts for the quantification of common functional groups, ligands, and biomolecules on different types of organic and inorganic nano-materials, using different types of optical reporters and method validation with the aid of multimodal reporters and mass balances. T2 - RSC Symposium on Nanoparticle concentration – critical needs and state-of-the-art measurement CY - London, UK DA - 24.04.2018 KW - PEG ligands KW - Surface group analysis KW - Upconverting nanoparticles KW - Integrating sphere spectroscopy KW - Absolute fluorescence quantum yield KW - Fluorescence decay kinetics PY - 2018 N1 - Geburtsname von Nirmalananthan-Budau, Nithiya: Nirmalananthan, N. - Birth name of Nirmalananthan-Budau, Nithiya: Nirmalananthan, N. AN - OPUS4-44986 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - CONF A1 - Resch-Genger, Ute A1 - Nirmalananthan-Budau, Nithiya A1 - Moser, Marko A1 - Geißler, Daniel A1 - Behnke, Thomas A1 - Schneider, Ralf T1 - Simple and Versatile Methods for Quantifying Functional Groups, Ligands, and Biomolecules on Nanomaterials N2 - Many applications of nanomaterials in the life sciences require the controlled functionalization of these materials with ligands like polyethylene glycol (PEG) and/or biomolecules such as peptides, proteins, and DNA. This enables to tune their hydrophilicity and biocompatibility, minimize unspecific interactions, improve biofunctionalization efficiencies, and enhance blood circulation times. Moreover, it is the ultimate prerequisite for their use as reporters in assays or the design of targeted optial probes for bioimaging. At the core of these functionalization strategies are reliable and validated methods for surface group and ligand quantification that can be preferably performed with routine laboratory instrumentation, require only small amounts of substances, and are suitable for many different types of nanomaterials. We present here versatile and simple concepts for the quantification of common functional groups, ligands, and biomolecules on different types of organic and inorganic nano-materials, using different types of optical reporters and method validation with the aid of multimodal reporters, method comparisons, and mass balances. T2 - INNANOPART open day CY - London, UK DA - 24.04.2018 KW - Surface functionalization KW - Nanoparticles KW - Surface chemistry KW - Multimodal reporters PY - 2018 N1 - Geburtsname von Nirmalananthan-Budau, Nithiya: Nirmalananthan, N. - Birth name of Nirmalananthan-Budau, Nithiya: Nirmalananthan, N. AN - OPUS4-44849 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -