TY - JOUR A1 - Handrea-Haller, Marlene A1 - Sahre, Mario A1 - Kautek, Wolfgang A1 - Neubauer, A. A1 - Sleytr, U.B. T1 - Electrochemistry of nano-scale bacterial surface protein layers on gold JF - Bioelectrochemistry N2 - The mechanism of the recrystallization of nano-scale bacterial surface protein layers (S-layers) on solid substrates is of fundamental interest in the understanding and engineering of biomembranes and e.g. biosensors. In this context, the influence of the charging state of the substrate had to be clarified. Therefore, the electrochemical behaviour of the S-layers on gold electrodes has been investigated by in-situ electrochemical quartz microbalance (EQMB) measurements, scanning force microscopy (SFM) and small-spot X-ray photoelectron spectroscopy (SS-XPS) of potentiostatically emersed substrates. It was shown that the negatively charged bonding sites of the S-layer units (e.g. carboxylates) can bond with positively charged Au surface atoms in the positively charged electrochemical double layer region positive of the point of zero charge (not, vert, similar-0.8 V vs. saturated mercury-mercurous sulphate electrode). Surface conditions in other potential regions decelerated the recrystallization and fixation of S-layers. Time-resolved in-situ and ex-situ measurements demonstrated that two-dimensional S-layer crystal formation on gold electrodes can occur within few minutes in contrast to hours common in self-assembled monolayer (SAM) generation. These results proved that the recrystallization and fixation of 2D-crystalline S-layers on an electronic conductor can be influenced and controlled by direct electrochemical manipulation. KW - S-layers crystallization KW - Gold KW - Electrochemical quartz microbalance KW - Scanning force microscopy KW - XPS PY - 2003 DO - https://doi.org/10.1016/S1567-5394(03)00047-1 SN - 0302-4598 SN - 1567-5394 VL - 61 SP - 1 EP - 8 PB - Elsevier Science CY - Oxford AN - OPUS4-2844 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER - TY - JOUR A1 - Handrea-Haller, Marlene A1 - Sahre, Mario A1 - Neubauer, A. A1 - Sleytr, U.B. A1 - Kautek, Wolfgang T1 - Electrochemistry of Nano-Scale Bacterial Surface Protein Layers on Gold JF - Electrochimica acta KW - S-layers crystallization KW - Gold KW - Electrochemical quartz microbalance KW - Scanning force microscopy KW - XPS PY - 2002 SN - 0013-4686 SN - 1873-3859 N1 - Geburtsname von Handrea-Haller, Marlene: Handrea, M. - Birth name of Handrea-Haller, Marlene: Handrea, M. PB - Elsevier Science CY - Kidlington AN - OPUS4-1736 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -