TY - JOUR A1 - Kamenz, C. A1 - Weidemann, Gerd T1 - Heavy metal - A contrasting substance for micro-tomographical visualization of scorpion book lungs JF - Micron N2 - Remarkable results obtained while searching for suitable contrasting agents for tomographical imaging are presented. Book lungs, the respiratory organs of scorpions (Scorpiones, Arachnida), served as challenging objects in terms of fragility and preparatory requirements, as well in terms of size differences between the entire organ and its functional subunits (the lamellae). The problem of tissue contrast was investigated using various metals, but most successfully addressed using osmium. Gaseous osmium tetroxide exposure with subsequent ethanol treatment yielded the best results. A constant and thorough staining of the book lungs was achieved, easing book-lung visualization using 3D-reconstructions from µCT scans. KW - Osmium tetroxide KW - muCT KW - X-ray KW - Contrast KW - Respiratory organ PY - 2009 DO - https://doi.org/10.1016/j.micron.2009.05.007 SN - 0047-7206 SN - 0968-4328 VL - 40 IS - 8 SP - 911 EP - 917 PB - Pergamon Press CY - Oxford AN - OPUS4-20095 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -