TY - JOUR A1 - Brenneis, Rudolf A1 - Baeck, Burkhard T1 - Esterification of fatty acids using Candida antarctica lipase A in water-abundant systems JF - Biotechnology letters N2 - The feasibility of using native lipase A from Candida antarctica (CAL-A) to esterify fatty acids with water-insoluble alcohols in the presence of excess water was investigated in stirred-tank reactors. For high reaction rates, a ratio of water:substrates of 0.6–1.4:1 (v/v) was required. CAL-A showed higher substrate selectivity for the esterification of saturated palmitic acid with branched-chain 2-ethyl-1-hexanol than for unsaturated oleic acid with linear alcohol (1-decanol). After 18 h at 70 °C in a 1.5 l bulk stirredtank reactor, an 2-ethyl-1-hexyl palmitic acid ester was obtained near 100 % yield [molar ratio palmitic acid:2-ethyl-1-hexanol ~1:1.25, with 1.11 % (w/w) Novocor ADL (based on palmitic acid weight)]. KW - Candida antarctica lipase A KW - Enzymatic esterification KW - 2-Ethyl-1-hexyl palmitic acid ester KW - Fatty acids KW - Water-abundant system PY - 2012 DO - https://doi.org/10.1007/s10529-012-0928-1 SN - 0141-5492 SN - 1573-6776 VL - 34 IS - 8 SP - 1459 EP - 1463 PB - Kluwer Academic Publ. CY - Dordrecht [u.a.] AN - OPUS4-26231 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -