64726 2025 eng lecture 0 -- -- -- Engineered nanoparticles (NPs) with various chemical compositions and surface functionalities are routinely fabricated for industrial applications such as medical diagnostics, drug delivery, sensing, catalysis, energy conversion and storage, opto-electronics, and information storage. NP function, interaction with biological species, and environmental fate are largely determined by surface functionalities. This calls for reliable, reproducible, and standardized surface characterization methods, which are vital for quality control of NPs, and mandatory to meet increasing concerns regarding their safety. Validated and standardized workflows for surface analysis are also increasingly requested by industry, international standardization organizations, regulatory agencies, and policymakers. To establish comparable measurements of surface functionalities across different labs and ease instrument performance validation, reference test materials and reference materials of known surface chemistry as well as reference data are needed. In the following, different methods for determining surface functionalities on ligand-stabilized core and core/shell NPs include advanced techniques are presented and discussed regarding method-inherent advantages and limitations. Special emphasis is dedicated to traceable quantitative nuclear magnetic resonance (qNMR), X-ray electron spectroscopy (XPS) and time of flight secondary ion mass spectrometry (ToF-SIMS), and simpler optical and electrochemical methods. LNE Workshop Paris, France 04.11.2025 1 publish Ute Resch-Genger eng uncontrolled Quality assurance eng uncontrolled Fluorescence eng uncontrolled Nano eng uncontrolled Particle eng uncontrolled Synthesis eng uncontrolled Characterization eng uncontrolled Advanced material eng uncontrolled Surface eng uncontrolled Standardization eng uncontrolled Reference material eng uncontrolled Functional group eng uncontrolled Quantification eng uncontrolled Coating eng uncontrolled Automation eng uncontrolled Potentiometry eng uncontrolled Method eng uncontrolled Validation eng uncontrolled Optical assay eng uncontrolled Fluram eng uncontrolled Fluorescamine eng uncontrolled qNMR eng uncontrolled Comparison eng uncontrolled ILC Analytische Chemie Ingenieurwissenschaften und zugeordnete Tätigkeiten 1 Analytische Chemie; Referenzmaterialien 1.2 Biophotonik 6 Materialchemie 6.1 Oberflächen- und Dünnschichtanalyse Umwelt Material Chemie und Prozesstechnik Chemische Charakterisierung und Spurenanalytik Datei im Netzwerk der BAM verfügbar ("Closed Access") Präsentation Advanced Materials