63082 2025 eng 1 8 article Wiley VHC-Verlag 1 -- -- -- AbstractDetection and monitoring of faecal contaminants in water is an important component of water quality testing protocol worldwide. However, a systematic overview of the faecal indicator paradigm, including its fundamentals and challenges in analytical applications, is missing. In particular, with respect to the advantages of using faecal indication pigments (FIP) over faecal indication bacteria (FIB). This discussion is based on two FIPs, Urobilin (UB) and Stercobilin (SB), which can enable rapid and real‐time indication of faecal contaminants in ground/surface water. Novel strategies for enhancing sensitive fluorescence‐based techniques for trace concentration detection have been discussed in detail, with specific reference to understanding their physicochemical properties, photophysics, metal‐ligand complexation, molecular aggregations, thermodynamics, fluorescence response and matrix interferences in aqueous media or environmental samples. The insights provided in this perspective article could inspire procedures by avoiding ambiguities and misinterpretations. 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In particular, with respect to the advantages of using faecal indication pigments (FIP) over faecal indication bacteria (FIB). This discussion is based on two FIPs, Urobilin (UB) and Stercobilin (SB), which can enable rapid and real\u2010time indication of faecal contaminants in ground\/surface water. Novel strategies for enhancing sensitive fluorescence\u2010based techniques for trace concentration detection have been discussed in detail, with specific reference to understanding their physicochemical properties, photophysics, metal\u2010ligand complexation, molecular aggregations, thermodynamics, fluorescence response and matrix interferences in aqueous media or environmental samples. 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