62935 2025 eng 1 12 15 article Springer Nature London 1 -- -- -- Nanopesticides have been recently introduced as novel pesticides to overcome the drawbacks of using traditional synthetic pesticides. The present study evaluated the acaricidal activity of Copper/Graphene oxide core-shell nanoparticles against two tick species, Rhipicephalus rutilus and Rhipicephalus turanicus. The Copper/Graphene oxide core-shell nanoparticles were synthetized through the solution plasma (SP) method under different conditions. The nanoparticles synthesized at 180 W and 45 min were highly toxic to Rh. rutilus and Rh. turanicus, with 50% lethal concentration (LC50) values of 248.1 and 195.7 mg ml−1, respectively, followed by those which were synthesized at 120 W/30 mins (LC50 = 581.5 and 526.5 mg ml−1), 120 W/15 mins (LC50 = 606.9 and 686.7 mg ml−1), and 100/45 mins (LC50 = 792.9 and 710.7 mg ml−1), after 24 h of application. The enzyme assays revealed that 180 W/45 min treatment significantly inhibited the activity of acetylcholinesterase (115 ± 0.81 and 123 ± 0.33 U/ mg protein/min) and superoxide dismutase (290 ± 0.18 and 310 ± 0.92 U/ mg protein/min) in Rh. rutilus and Rh. turanicus, respectively, as compared with the negative control. The results also revealed a significantly increased catalase activity (895 ± 0.37 and 870 ± 0.31 U/ mg protein/min) in Rh. rutilus and Rh. turanicus, respectively. The above results indicated that Copper/Graphene oxide core-shell nanoparticles could be a promising alternatives for the management of ticks. Scientific reports 10.1038/s41598-025-86560-4 2045-2322 urn:nbn:de:kobv:b43-629359 true ok journal-article publish 10.04.2025 Haytham Senbill Amr Gangan Ahmed M. Saeed Mohammed E. Gad Jehan Zeb Alaa Fahmy eng uncontrolled Copper/graphene oxide eng uncontrolled Nanopesticides eng uncontrolled Rhipicephalus rutilus eng uncontrolled Rhipicephalus turanicus eng uncontrolled Acetylcholinesterase eng uncontrolled Antioxidants Ingenieurwissenschaften und zugeordnete Tätigkeiten 6 Materialchemie 6.1 Oberflächen- und Dünnschichtanalyse Material Verlagsliteratur Datei für die Öffentlichkeit verfügbar ("Open Access") Wissenschaftliche Artikel der BAM Advanced Materials Bundesanstalt für Materialforschung und -prüfung (BAM) https://opus4.kobv.de/opus4-bam/files/62935/s41598-025-86560-4.pdf