46463 2019 eng 527 534 281 article Elsevier B.V. 1 -- -- -- We report polyethylene glycol (PEG)-grafting antifouling surfaces using a plasma copolymerized (PcP) technique to monitor protease activity in complex media. By varying the mixing ratio of the PEG and ethylenediamine (EDA) precursors, the PcP-PEG-EDA (PcP-PE) film was able to easily control surface amine density with good preservation of the internal PEG structure. We found that nonspecific protein adsorption was dramatically reduced in serum-containing media on the PcP-PE films, as opposed to that on plasma polymerized-EDA (PP-E) films without PEG. When SPR sensor chips coated with PcP-PE film were employed to detect protease activity, biotinylated luciferase probes (luciferase-peptide-biotin) on streptavidin-conjugated SPR chips enabled real-time and label-free measurement of matrix metalloproteinase activity in cell culture media. Owing to its excellent antifouling ability, this newly developed method boasts minimal nonspecific binding and can serve as a biochip platform to promote a wide range of applications in the biological field. Sensors & Actuators: B. Chemical 10.1016/j.snb.2018.10.123 0925-4005 21.01.2019 J. Park G. B. Kim Andreas Lippitz Y. M. Kim D. Jung Wolfgang Unger Y.-P. Kim T. G. Lee eng uncontrolled Polyethylene glycol (PEG)-grafting antifouling surface eng uncontrolled Biochip eng uncontrolled ATR-FTIR spectroscopy eng uncontrolled XPS eng uncontrolled NEXAFS eng uncontrolled Plasma Analytische Chemie Verlagsliteratur Datei im Netzwerk der BAM verfügbar ("Closed Access")