45161 2018 eng lecture 0 -- -- -- An overview about different analytical approaches will be presented of how to detect metals in individual biological cells by use of ICP-MS. For this purpose, we are using different sample introduction systems for ICP-MS for detection, imaging and quantification of metals at cellular levels. By use of laser ablation, we have studied the up-take by and distribution of nanoparticles in single cells. Recently we have developed staining techniques to measure protein and DNA content of cells and identifying the cell status by immunoassays using metal-tagging of antibodies. New research based on cell arrays will be shortly discussed. Using pneumatic nebulization and microdroplet generation, we have also studied the up-take of nanoparticles and toxic metals as well as essential elements in single cells using different ICP-MS mass spectrometric concepts (sector field instrument, triple-quad instrument, time of flight (CyTOF) instrument). The different ICP-MS based methods will be compared concerning their analytical figures of merit and their strengths and weaknesses will be evaluated. 16th Czech-Slovak Spectroscopic Conference Luhacovice, Czech Republic 27.05.2018 31.05.2018 0 Norbert Jakubowski eng uncontrolled Single cell analysis eng uncontrolled ICP-MS eng uncontrolled Nanoparticles Analytische Chemie Datei im Netzwerk der BAM verfügbar ("Closed Access") Präsentation